FastQCFastQC Report
Fri 27 May 2016
SRR2139720_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139720_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9064048
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79500.042.6098631
GTATCAA135750.030.8482151
TCAACGC156750.025.8079454
ATCAACG159650.025.3900343
CAACGCA161050.025.1693155
AACGCAG166950.024.3284936
TATCAAC173950.023.6301962
GTACATG207150.021.1370811
TACATGG210350.020.3521042
ACGCAGA199250.020.3438847
CGCAGAG204550.019.7770488
ACATGGG212200.019.5808663
CATGGGG155250.017.083754
AGAGTAC219100.015.92479910-11
GCAGAGT252750.015.7644749
GAGTACT144500.015.50246912-13
CAGAGTA246800.014.73817310-11
GAGTACA150050.014.1301161
ATGGGGG91400.014.0868775
TATACTG46850.013.8711365
Produced by FastQC (version 0.11.5)