##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139720_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9064048
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14461518738648	33.0	31.0	34.0	30.0	34.0
2	32.26694220948521	34.0	31.0	34.0	30.0	34.0
3	32.321598252789485	34.0	31.0	34.0	30.0	34.0
4	35.95313881832929	37.0	35.0	37.0	35.0	37.0
5	35.81655812061013	37.0	35.0	37.0	35.0	37.0
6	35.83607136678888	37.0	35.0	37.0	35.0	37.0
7	35.7497632404418	37.0	35.0	37.0	33.0	37.0
8	35.711008811956866	37.0	35.0	37.0	33.0	37.0
9	37.33917814645289	39.0	37.0	39.0	34.0	39.0
10-11	37.23628443935866	39.0	37.0	39.0	33.0	39.0
12-13	37.19945729546004	39.0	37.0	39.0	33.0	39.0
14-15	38.39440926394035	40.0	38.0	41.0	33.0	41.0
16-17	38.41019349191443	40.0	38.0	41.0	33.0	41.0
18-19	38.401364820662906	40.0	38.0	41.0	33.5	41.0
20-21	38.37186561387699	40.0	38.0	41.0	34.0	41.0
22-23	38.26972188381437	40.0	38.0	41.0	33.0	41.0
24-25	38.18221995989956	40.0	38.0	41.0	33.0	41.0
26-27	38.015169903173685	40.0	37.0	41.0	33.0	41.0
28-29	37.879304544982446	40.0	37.0	41.0	33.0	41.0
30-31	37.731127076544766	40.0	37.0	41.0	32.0	41.0
32-33	37.92952424782514	40.0	37.0	41.0	33.0	41.0
34-35	37.91594785729205	40.0	37.0	41.0	33.0	41.0
36-37	37.832980795073915	40.0	37.0	41.0	33.0	41.0
38-39	37.7236514636754	40.0	37.0	41.0	33.0	41.0
40-41	37.6778306566079	40.0	37.0	41.0	33.0	41.0
42-43	37.573224928357526	40.0	37.0	41.0	32.0	41.0
44-45	37.61584845831072	40.0	37.0	41.0	32.5	41.0
46-47	37.75005385266868	40.0	37.0	41.0	33.0	41.0
48-49	37.68100408872487	40.0	37.0	41.0	32.0	41.0
50-51	37.56150028632479	40.0	36.5	41.0	32.0	41.0
52-53	37.415489571651165	39.5	36.0	41.0	31.0	41.0
54-55	37.312581426177616	39.0	36.0	41.0	31.0	41.0
56-57	37.14682112524562	39.0	36.0	41.0	31.0	41.0
58-59	37.000724713035616	39.0	35.0	40.0	31.0	41.0
60-61	36.7737787574906	38.5	35.0	40.0	31.0	41.0
62-63	36.516799367224905	38.0	35.0	40.0	30.5	41.0
64-65	36.30057597841518	38.0	35.0	40.0	30.0	41.0
66-67	36.00937335877063	37.5	34.0	40.0	30.0	41.0
68-69	35.69025265942899	37.0	34.0	39.5	29.0	41.0
70-71	35.38642255596554	36.0	34.0	39.0	29.0	41.0
72-73	35.07640674010013	36.0	34.0	39.0	29.0	40.0
74-75	34.68122405909831	35.5	33.5	38.0	29.0	40.0
76-77	33.61084985077873	35.0	32.0	37.0	27.5	39.0
78-79	33.94610644063485	35.0	33.0	37.0	28.5	39.0
80-81	33.81643440989901	35.0	33.0	36.5	29.0	39.0
82-83	33.580370818668	35.0	33.0	36.0	29.0	37.5
84-85	33.35261501557426	35.0	33.0	36.0	29.0	37.0
86-87	33.13739645570308	35.0	32.0	35.0	28.5	37.0
88-89	32.90368772618497	34.0	32.0	35.0	28.0	36.0
90-91	32.757679190319365	34.0	32.0	35.0	27.0	36.0
92-93	32.66611644530712	34.0	32.0	35.0	27.0	36.0
94-95	32.61126547621853	34.0	32.0	35.0	27.0	35.0
96-97	32.54751250649281	34.0	32.0	35.0	27.0	35.0
98-99	32.67478607793494	34.0	32.0	35.0	29.0	35.0
100-101	31.768769778720774	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	10.0
20	48.0
21	325.0
22	1738.0
23	5735.0
24	15001.0
25	31390.0
26	56212.0
27	89194.0
28	126933.0
29	169160.0
30	215473.0
31	267850.0
32	330963.0
33	418349.0
34	550558.0
35	749170.0
36	1123311.0
37	1764799.0
38	2015090.0
39	1088237.0
40	44498.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.986002721962635	21.72128832503976	14.142544258371096	25.150164694626508
2	14.475986276169905	22.873061191256518	39.65237200326304	22.998580529310537
3	17.140619731934343	27.07304727424215	31.502392749906	24.283940243917506
4	12.212522826506197	18.86707272619755	39.02009747547005	29.9003069718262
5	12.53652893276823	38.34184240860154	36.53631357645061	12.58531508217962
6	29.70298700977753	39.04169527787143	18.63120098216603	12.624116730185012
7	25.765287209423427	32.83233936978268	24.15158216284821	17.25079125794568
8	24.2553768470776	37.08471093710007	21.754353021961048	16.905559193861286
9	25.48274236853115	16.03120371825039	21.36447203280477	37.12158188041369
10-11	23.745941107107992	26.94974144002768	29.838108756705612	19.466208696158713
12-13	24.42644831536638	24.549853442965	29.639527504708713	21.38417073695991
14-15	21.606372781785797	26.06205306944535	26.795224385396015	25.536349763372833
16-17	21.494783908812835	29.028033321596077	27.67298987177632	21.80419289781477
18-19	21.527224922021595	28.450864337876407	28.953156470486473	21.068754269615518
20-21	22.17424070947861	27.78906518377202	28.711821427273044	21.32487267947633
22-23	21.91118166446891	27.75783602678004	28.566038312547732	21.764943996203314
24-25	21.774658345990865	27.872799703150452	28.622660472147064	21.72988147871162
26-27	21.7906371373126	28.04280339313368	28.52102213597379	21.645537333579934
28-29	21.745069088394718	27.99423280927798	28.57082481677546	21.689873285551847
30-31	22.011476995132313	27.919842632448265	28.477463625131822	21.591216747287604
32-33	21.794301660270808	28.053120629710698	28.454942360672565	21.69763534934593
34-35	21.886071221774923	28.076277044748853	28.47119248064423	21.56645925283199
36-37	21.880187418960826	27.958185889491038	28.443901954590565	21.717724736957567
38-39	21.708512705648694	28.00787989863267	28.585341705994544	21.698265689724096
40-41	21.894670728963934	27.851799478421324	28.520580336126322	21.732949456488416
42-43	21.777953750566535	27.957333226872745	28.36292383852575	21.90178918403497
44-45	21.824452731281827	28.030130193852482	28.21398167495123	21.931435399914463
46-47	21.89026411575298	27.960289713478552	28.059420015028557	22.09002615573991
48-49	21.773645219455496	28.07375726323341	28.11328099034483	22.03931652696626
50-51	21.761929773891982	28.153123004692105	28.02042176792555	22.064525453490365
52-53	21.872809488362716	28.0893948910722	27.931165975576533	22.10662964498855
54-55	21.82622093900896	27.986636902535867	27.979870635928588	22.207271522526582
56-57	21.87167106582991	28.08634734275402	27.869319443447278	22.172662147968794
58-59	21.79934986194674	28.122064995340924	27.893061808957718	22.18552333375462
60-61	21.93506558083158	28.108149123016606	27.843307306785004	22.113477989366807
62-63	21.854338940054248	28.2386283601967	27.824108081354392	22.08292461839466
64-65	21.78121584538393	28.203298237899947	27.756913284512123	22.258572632204
66-67	21.891220657159824	28.302805813866662	27.53809281715886	22.26788071181465
68-69	21.876562618471073	28.321491881685024	27.534600580493695	22.267344919350208
70-71	21.90906021014172	28.059421737463474	27.62592482801652	22.40559322437829
72-73	21.932845642916245	28.07840499212314	27.52591629994979	22.462833065010823
74-75	21.930806089955496	28.154386863031426	27.48908932040778	22.425717726605303
76-77	22.142665023835566	28.138008058752558	27.400819927389986	22.318506990021884
78-79	22.050014382990554	28.13872932403221	27.34503993839627	22.466216354580965
80-81	22.04739933283647	28.1870409457868	27.368861024033436	22.396698697343293
82-83	22.06391936596282	28.138506592291307	27.476951170765833	22.32062287098004
84-85	22.137974684540787	28.190784631238042	27.453890486510574	22.2173501977106
86-87	22.007288521088743	28.196907364795187	27.526991781379344	22.268812332736722
88-89	22.025977802771777	28.180017773388798	27.522154852084324	22.271849571755098
90-91	22.07104770013114	28.202217514674118	27.608991109933527	22.11774367526122
92-93	22.063678976374053	28.1882480463161	27.626125107437826	22.121947869872024
94-95	21.984854311133088	28.18691013319127	27.711632533667036	22.11660302200861
96-97	21.80761574584374	28.252436041133215	27.826309399164078	22.113638813858962
98-99	21.942222776335534	28.77205227898621	27.999651486261868	21.28607345841639
100-101	24.68585884210577	36.532160441487584	29.161070493623725	9.62091022278292
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2071.0
1	1895.0
2	2374.5
3	3214.0
4	4180.0
5	6158.0
6	7733.5
7	8789.5
8	9398.5
9	9307.5
10	8940.0
11	8528.0
12	9527.5
13	12698.5
14	19281.5
15	27881.0
16	34736.5
17	38514.0
18	39768.0
19	39482.0
20	38673.5
21	38775.5
22	41206.0
23	47441.0
24	57512.0
25	71868.0
26	90470.0
27	110970.5
28	133854.5
29	158195.5
30	181045.0
31	203194.0
32	226161.0
33	248274.5
34	268425.0
35	287885.5
36	309218.5
37	327859.5
38	342958.5
39	357112.5
40	369881.5
41	382046.5
42	389628.0
43	395820.0
44	401691.5
45	404157.0
46	404677.0
47	402908.5
48	401175.5
49	394592.5
50	382567.0
51	369061.5
52	351624.5
53	330926.0
54	307929.0
55	283237.0
56	258206.5
57	232461.0
58	204643.0
59	175614.5
60	146996.0
61	120090.0
62	95832.0
63	74756.0
64	56329.5
65	42009.5
66	31434.5
67	23776.5
68	17993.5
69	13142.5
70	9547.5
71	6956.0
72	4952.5
73	3364.0
74	2286.5
75	1583.5
76	1082.0
77	737.0
78	524.5
79	380.5
80	268.0
81	183.0
82	127.5
83	85.0
84	57.5
85	41.5
86	34.5
87	28.0
88	22.5
89	17.5
90	12.0
91	8.5
92	5.5
93	2.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0011694554132987822
3	0.0
4	2.6478235772802616E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.003491817342538345
18-19	0.0
20-21	0.002179035756266631
22-23	0.0
24-25	3.752545660754862E-4
26-27	0.0037374969822610224
28-29	0.0
30-31	1.8259401018608985E-4
32-33	8.824094506385148E-4
34-35	8.973243571172592E-4
36-37	0.010120631873011427
38-39	0.011632689589914577
40-41	0.02583170285704232
42-43	0.002203777921491277
44-45	0.0013420061942338078
46-47	0.0016905657093927004
48-49	0.0015988888500977029
50-51	7.274949439101399E-5
52-53	1.349184699951135E-4
54-55	0.006772009869274164
56-57	0.004189503754222479
58-59	0.004520391262147358
60-61	0.0019889217060970396
62-63	5.63177036207503E-4
64-65	8.900254646912617E-4
66-67	7.487276688600116E-4
68-69	6.855468555850988E-6
70-71	6.968394914549011E-6
72-73	1.4165085429984357E-4
74-75	2.6632849186524816E-4
76-77	0.0016979373793375803
78-79	0.002077541287223732
80-81	0.011491035854307318
82-83	0.010077002518323297
84-85	0.013672162634664309
86-87	0.006762881966450329
88-89	0.09490779940667476
90-91	4.5205651811503487E-4
92-93	4.28636667072157E-5
94-95	2.7284380343557874E-4
96-97	0.015900099628475266
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	257.0
20-21	791.0
22-23	1708.0
24-25	3332.0
26-27	5327.0
28-29	10641.0
30-31	24800.0
32-33	34858.0
34-35	50350.0
36-37	77829.0
38-39	80969.0
40-41	83835.0
42-43	93379.0
44-45	109208.0
46-47	110482.0
48-49	103849.0
50-51	97513.0
52-53	87708.0
54-55	100310.0
56-57	105962.0
58-59	110728.0
60-61	109358.0
62-63	105227.0
64-65	112738.0
66-67	119965.0
68-69	118272.0
70-71	116835.0
72-73	113082.0
74-75	114503.0
76-77	115221.0
78-79	122034.0
80-81	123028.0
82-83	118165.0
84-85	118624.0
86-87	125048.0
88-89	131134.0
90-91	139137.0
92-93	146613.0
94-95	182062.0
96-97	234092.0
98-99	720258.0
100-101	4584816.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.01883140647782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.89616206137615	80.8859279538263
2	5.773956546552691	10.164338156387004
3	1.2908350614678366	3.408533809467238
4	0.48923592654454334	1.7224789814606452
5	0.21214902403464742	0.9336554589777221
6	0.11052201263045977	0.583681103785503
7	0.06395747862002811	0.3940623769487765
8	0.04414955961022229	0.31087941152019183
9	0.026689545473173173	0.21142643429868774
>10	0.09023234630028724	1.236391933415434
>50	0.0018647157150000543	0.1083703635204946
>100	2.45721674897338E-4	0.04025401639203334
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1032598238667757E-5	0.0	0.0	0.0
3	0.0	1.1032598238667757E-5	0.0	1.1032598238667757E-5	0.0
4	0.0	5.516299119333878E-5	0.0	3.309779471600327E-5	2.2065196477335513E-5
5	1.1032598238667757E-5	6.619558943200654E-5	0.0	4.4130392954671026E-5	2.2065196477335513E-5
6	2.2065196477335513E-5	6.619558943200654E-5	0.0	4.4130392954671026E-5	3.309779471600327E-5
7	2.2065196477335513E-5	7.722818767067429E-5	0.0	5.516299119333878E-5	3.309779471600327E-5
8	2.2065196477335513E-5	8.826078590934205E-5	0.0	5.516299119333878E-5	5.516299119333878E-5
9	2.2065196477335513E-5	1.765215718186841E-4	0.0	7.722818767067429E-5	5.516299119333878E-5
10-11	2.758149559666939E-5	1.8755417005735187E-4	0.0	1.268748797446792E-4	5.516299119333878E-5
12-13	3.309779471600327E-5	2.0410306741535349E-4	0.0	1.3239117886401308E-4	5.516299119333878E-5
14-15	4.4130392954671026E-5	2.9236385332469554E-4	0.0	1.3790747798334696E-4	8.274448679000817E-5
16-17	8.826078590934205E-5	2.9788015244402945E-4	0.0	1.5445637534134858E-4	1.1584228150601143E-4
18-19	8.826078590934205E-5	3.1442904980203106E-4	0.0	1.8203787093801799E-4	1.4342377710268084E-4
20-21	8.826078590934205E-5	3.309779471600327E-4	0.0	2.9788015244402945E-4	1.4342377710268084E-4
22-23	9.377708502867593E-5	3.695920409953698E-4	0.0	5.626625101720557E-4	1.599726744606825E-4
24-25	1.1032598238667757E-4	4.026898357113731E-4	0.0	9.212219529287577E-4	1.765215718186841E-4
26-27	1.1032598238667757E-4	4.302713313080425E-4	0.0	0.0015997267446068246	1.765215718186841E-4
28-29	1.1032598238667757E-4	4.357876304273764E-4	0.0	0.005698336990271896	1.765215718186841E-4
30-31	1.1032598238667757E-4	4.964669207400491E-4	0.0	0.020697154295740712	1.765215718186841E-4
32-33	1.4894007622201472E-4	5.074995189787168E-4	0.0	0.05045207174542765	1.765215718186841E-4
34-35	1.6548897358001634E-4	5.847277066493911E-4	0.0	0.0905004033517916	1.8755417005735187E-4
36-37	1.6548897358001634E-4	6.729884925587332E-4	0.0	0.1467887195654745	1.8755417005735187E-4
38-39	1.6548897358001634E-4	7.005699881554025E-4	0.0	0.232859534724441	2.0410306741535349E-4
40-41	1.765215718186841E-4	7.447003811100736E-4	0.0	0.340912801873953	2.0961936653468737E-4
42-43	1.8755417005735187E-4	7.777981758260769E-4	0.0	0.40702564681916953	2.1513566565402125E-4
44-45	1.8755417005735187E-4	8.495100643774172E-4	0.0	0.5003503953200601	2.2065196477335513E-4
46-47	1.9307046917668572E-4	8.936404573320882E-4	0.0	0.6038306504996442	2.316845630120229E-4
48-49	2.0961936653468737E-4	8.991567564514221E-4	0.0	0.6853615514833991	2.4271716125069063E-4
50-51	2.3720086213135678E-4	0.0010315479353154352	0.0	0.7596661006208263	2.4271716125069063E-4
52-53	2.482334603700245E-4	0.0010591294309121046	0.0	0.8329887485150123	2.537497594893584E-4
54-55	2.537497594893584E-4	0.001086710926508774	0.0	0.9804283913765681	2.7029865684736E-4
56-57	2.6478235772802616E-4	0.0010922272256281079	0.0	1.1022393085296989	2.758149559666939E-4
58-59	2.7029865684736E-4	0.0010922272256281079	0.0	1.1990007113819345	2.758149559666939E-4
60-61	2.758149559666939E-4	0.0011032598238667757	0.0	1.2477813444942039	2.758149559666939E-4
62-63	2.868475542053617E-4	0.0011142924221054435	0.0	1.2771335721081796	2.868475542053617E-4
64-65	2.868475542053617E-4	0.001224618404492121	0.0	1.298818143946281	2.9236385332469554E-4
66-67	2.9236385332469554E-4	0.001224618404492121	0.0	1.3105678610704623	2.9788015244402945E-4
68-69	3.0891275068269716E-4	0.0012521999000887902	0.0	1.3217107852915166	2.9788015244402945E-4
70-71	3.0891275068269716E-4	0.0012852976948047937	0.0	1.3317339007913462	3.0891275068269716E-4
72-73	3.0891275068269716E-4	0.0013073628912821291	0.0	1.3395560129425617	3.309779471600327E-4
74-75	3.0891275068269716E-4	0.0013459769851174664	0.0	1.346567229123235	3.309779471600327E-4
76-77	3.0891275068269716E-4	0.0013956236771914712	0.0	1.352778581931605	3.309779471600327E-4
78-79	3.199453489213649E-4	0.0014121725745494728	0.0	1.3578756423178695	3.309779471600327E-4
80-81	3.199453489213649E-4	0.0014121725745494728	0.0	1.3610199328158896	3.364942462793666E-4
82-83	3.199453489213649E-4	0.0014176888736688069	0.0	1.3630940612847593	3.530431436373682E-4
84-85	3.199453489213649E-4	0.0014287214719074745	0.0	1.3648813421994235	3.530431436373682E-4
86-87	3.199453489213649E-4	0.0014563029675041439	0.0	1.3652123201465836	3.530431436373682E-4
88-89	3.199453489213649E-4	0.0015114659586974825	0.0	1.3652950646333735	3.530431436373682E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7950	0.0	42.609863	1
GTATCAA	13575	0.0	30.848215	1
TCAACGC	15675	0.0	25.807945	4
ATCAACG	15965	0.0	25.390034	3
CAACGCA	16105	0.0	25.169315	5
AACGCAG	16695	0.0	24.328493	6
TATCAAC	17395	0.0	23.630196	2
GTACATG	20715	0.0	21.137081	1
TACATGG	21035	0.0	20.352104	2
ACGCAGA	19925	0.0	20.343884	7
CGCAGAG	20455	0.0	19.777048	8
ACATGGG	21220	0.0	19.580866	3
CATGGGG	15525	0.0	17.08375	4
AGAGTAC	21910	0.0	15.924799	10-11
GCAGAGT	25275	0.0	15.764474	9
GAGTACT	14450	0.0	15.502469	12-13
CAGAGTA	24680	0.0	14.738173	10-11
GAGTACA	15005	0.0	14.130116	1
ATGGGGG	9140	0.0	14.086877	5
TATACTG	4685	0.0	13.871136	5
>>END_MODULE