FastQCFastQC Report
Fri 27 May 2016
SRR2139719_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139719_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14882237
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131550.042.781571
GTATCAA225650.032.254271
TCAACGC266500.026.275374
ATCAACG275050.025.4585933
CAACGCA275550.025.4565435
AACGCAG284750.024.71956
TATCAAC295100.024.14132
GTACATG378550.022.2577041
TACATGG386150.021.4311312
ACATGGG385850.020.9536823
ACGCAGA343550.020.4178457
CGCAGAG355650.019.7573858
CATGGGG275900.018.7816124
AGAGTAC370500.016.56389810-11
GCAGAGT421150.016.5305469
GAGTACT228800.016.00294912-13
CAGAGTA410300.015.18446110-11
ATGGGGG170200.014.8654755
GAGTACA270850.014.4762371
GTACTTT259650.014.07126814-15
Produced by FastQC (version 0.11.5)