##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139719_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14882237
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12585251800519	33.0	31.0	34.0	30.0	34.0
2	32.249697945275294	33.0	31.0	34.0	30.0	34.0
3	32.30331945392349	34.0	31.0	34.0	30.0	34.0
4	35.94311554103056	37.0	35.0	37.0	35.0	37.0
5	35.80599153205261	37.0	35.0	37.0	35.0	37.0
6	35.824329702584365	37.0	35.0	37.0	35.0	37.0
7	35.73420420599403	37.0	35.0	37.0	33.0	37.0
8	35.69610307912715	37.0	35.0	37.0	33.0	37.0
9	37.32196161101318	39.0	37.0	39.0	34.0	39.0
10-11	37.21538512657741	39.0	37.0	39.0	33.0	39.0
12-13	37.175715115946616	39.0	37.0	39.0	33.0	39.0
14-15	38.362757494051465	40.0	38.0	41.0	33.0	41.0
16-17	38.3791138724642	40.0	38.0	41.0	33.0	41.0
18-19	38.37093294509421	40.0	38.0	41.0	33.5	41.0
20-21	38.3357204627017	40.0	38.0	41.0	34.0	41.0
22-23	38.23191036159159	40.0	38.0	41.0	33.0	41.0
24-25	38.14239664602089	40.0	37.5	41.0	33.0	41.0
26-27	37.972029446756565	40.0	37.0	41.0	33.0	41.0
28-29	37.83729287192009	40.0	37.0	41.0	33.0	41.0
30-31	37.68913101348316	40.0	37.0	41.0	32.0	41.0
32-33	37.88839802885074	40.0	37.0	41.0	33.0	41.0
34-35	37.8715702961899	40.0	37.0	41.0	33.0	41.0
36-37	37.78778117501181	40.0	37.0	41.0	33.0	41.0
38-39	37.67869554093312	40.0	37.0	41.0	32.5	41.0
40-41	37.62690810419865	40.0	37.0	41.0	32.0	41.0
42-43	37.51563607505878	39.5	36.5	41.0	32.0	41.0
44-45	37.55933010604852	39.5	36.5	41.0	32.5	41.0
46-47	37.696014523493865	40.0	37.0	41.0	32.5	41.0
48-49	37.62066319909004	40.0	37.0	41.0	32.0	41.0
50-51	37.4968362820253	40.0	36.0	41.0	32.0	41.0
52-53	37.340127494606776	39.0	36.0	41.0	31.0	41.0
54-55	37.2263006752885	39.0	36.0	41.0	31.0	41.0
56-57	37.0492228204959	39.0	35.0	40.5	31.0	41.0
58-59	36.8930908525538	39.0	35.0	40.0	31.0	41.0
60-61	36.6550406472591	38.0	35.0	40.0	31.0	41.0
62-63	36.380373484689756	38.0	35.0	40.0	30.0	41.0
64-65	36.14940207986057	37.5	34.5	40.0	30.0	41.0
66-67	35.84769879470471	37.0	34.0	40.0	29.5	41.0
68-69	35.520118075029785	36.5	34.0	39.0	29.0	41.0
70-71	35.21123937544608	36.0	34.0	39.0	29.0	40.5
72-73	34.89824779296306	36.0	34.0	39.0	29.0	40.0
74-75	34.50722874081407	35.0	33.0	38.0	29.0	40.0
76-77	33.44789589175019	34.5	31.5	36.5	27.5	39.0
78-79	33.78825602736097	35.0	33.0	37.0	28.0	39.0
80-81	33.66978993225616	35.0	33.0	36.0	29.0	38.5
82-83	33.44783448101185	35.0	33.0	36.0	28.5	37.0
84-85	33.22950465389982	35.0	32.0	35.5	28.0	37.0
86-87	33.02161250656632	34.5	32.0	35.0	28.0	36.5
88-89	32.79186853077906	34.0	32.0	35.0	27.0	36.0
90-91	32.658067462632246	34.0	32.0	35.0	27.0	36.0
92-93	32.57071632512788	34.0	32.0	35.0	27.0	35.5
94-95	32.52132587145552	34.0	32.0	35.0	27.0	35.0
96-97	32.46156361006315	34.0	32.0	35.0	27.0	35.0
98-99	32.59538034779196	34.0	32.0	35.0	28.5	35.0
100-101	31.66648988637028	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	8.0
19	15.0
20	95.0
21	658.0
22	2996.0
23	10038.0
24	26079.0
25	54187.0
26	96136.0
27	150631.0
28	214348.0
29	283286.0
30	359771.0
31	447643.0
32	554632.0
33	703481.0
34	927963.0
35	1273018.0
36	1912930.0
37	2966203.0
38	3177229.0
39	1659699.0
40	61190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.40382752942316	21.429560623177817	13.9163218540331	25.250289993365914
2	14.744055456868448	22.685346079962628	39.09941989182675	23.47117857134218
3	17.27955951783324	26.921295501476024	31.2045427041647	24.594602276526036
4	12.388482504662955	18.52718087131508	38.598240286344875	30.486096337677093
5	12.717516862552317	38.28708681362889	36.24935552363532	12.746040800183467
6	30.246064486138746	38.56910758779073	18.389648007890212	12.79517991818031
7	26.283380650368628	32.548399813818314	23.88026074305899	17.287958792754075
8	24.816591752973697	36.58616644796074	21.4482674882815	17.148974310784055
9	25.828146669079388	15.754291508729501	21.00846129516685	37.409100527024265
10-11	24.16032616601926	26.616324548520492	29.511887225018658	19.711462060441583
12-13	24.787459707838277	24.25158932759907	29.328400024808097	21.632550939754555
14-15	21.966761448564487	25.638850530333578	26.44374968628708	25.95063833481485
16-17	21.884211874839004	28.64189634886668	27.28617446982892	22.187717306465395
18-19	21.94945894222757	28.09957266505029	28.55538451645408	21.395583876268066
20-21	22.583965535935363	27.43938097360774	28.291178298689434	21.68547519176746
22-23	22.310709299336516	27.335395409556227	28.223282195859156	22.130613095248098
24-25	22.154492633587967	27.452686843973595	28.28027109447634	22.1125494279621
26-27	22.15024878355466	27.585802341128723	28.20791338537269	22.05603548994393
28-29	22.121484508033443	27.512044888034808	28.251749625961647	22.114720977970105
30-31	22.270926093326835	27.5121631126297	28.1317965266971	22.085114267346366
32-33	22.10300722299902	27.67352663816452	28.118653470370475	22.104812668465982
34-35	22.241583817667486	27.64947198087287	28.171943055111043	21.937001146348603
36-37	22.22104341249375	27.569993954346707	28.06165445276994	22.147308180389608
38-39	22.097780291622986	27.664632703561526	28.170133152421112	22.06745385239438
40-41	22.290500160125845	27.54298324292514	28.065941401499856	22.100575195449156
42-43	22.1840974778381	27.64682351481596	27.890173995136013	22.278905012209922
44-45	22.209598878253388	27.703739246744984	27.81325878092081	22.273403094080816
46-47	22.304918324495276	27.64182892459966	27.610728740805513	22.44252401009955
48-49	22.20859664636797	27.72616877642499	27.66573849438947	22.399496082817567
50-51	22.207791313230576	27.794049223278776	27.604599874769452	22.393559588721192
52-53	22.371381617874917	27.695369572185502	27.494128275564204	22.439120534375373
54-55	22.32257290257018	27.61562034671406	27.544839384755992	22.51696736595976
56-57	22.338170937475656	27.74989479111189	27.48897367427866	22.42296059713379
58-59	22.250822798364368	27.751117001315865	27.495511389233897	22.50254881108587
60-61	22.346711964941196	27.758569858225325	27.469022126369637	22.42569605046384
62-63	22.30135957978512	27.862507087677695	27.465044108608488	22.3710892239287
64-65	22.265776093281712	27.778230043313577	27.448884093383967	22.50710977002074
66-67	22.30870503323962	27.85125703050583	27.296926304946073	22.54311163130847
68-69	22.26907885088679	27.861987516078994	27.3128914225642	22.55604221047002
70-71	22.264916715264825	27.630848172020904	27.40787415052315	22.69636096219112
72-73	22.305255829871363	27.682249283936912	27.28687816935928	22.725616716832445
74-75	22.190290221053957	27.75691255119214	27.329416944873763	22.723380282880136
76-77	22.38911981255912	27.68178686436031	27.250438285830526	22.67865503725005
78-79	22.278012170466592	27.675911424855837	27.182071897933007	22.86400450674456
80-81	22.30529238022459	27.76385821113363	27.14462946464593	22.78621994399585
82-83	22.322346819508073	27.696814333334814	27.251230262054943	22.72960858510217
84-85	22.337423148175585	27.743764960269964	27.21752750619687	22.701284385357585
86-87	22.27489241372637	27.737061480784952	27.308281864564893	22.679764240923785
88-89	22.27139351052546	27.678944309678215	27.352280301984017	22.697381877812305
90-91	22.221926458165246	27.753693404655305	27.40616553832679	22.61821459885266
92-93	22.220229626395067	27.67502596291454	27.434551841356935	22.67019256933346
94-95	22.053370902140163	27.733975373179764	27.510452334951513	22.702201389728565
96-97	22.021004636403468	27.80731607762875	27.56186334083545	22.60981594513233
98-99	22.155601826415474	28.364517299874457	27.67772339550972	21.802157478200346
100-101	25.11349401754544	36.18848709838835	28.76514385038468	9.93287503368153
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3196.0
1	2973.5
2	3901.5
3	5358.0
4	6890.0
5	10121.0
6	12863.5
7	14750.5
8	15938.5
9	15741.0
10	14672.0
11	13661.0
12	14933.5
13	19595.5
14	30295.0
15	44782.5
16	56032.0
17	61728.0
18	63462.5
19	62760.5
20	61060.0
21	60822.5
22	63639.5
23	71996.0
24	87290.5
25	109577.5
26	137625.5
27	167569.0
28	200918.5
29	237314.0
30	271177.5
31	304910.5
32	341432.5
33	377069.0
34	411029.5
35	445072.0
36	482122.0
37	513399.0
38	537329.5
39	560042.5
40	581954.5
41	601865.5
42	614381.0
43	628557.5
44	642044.0
45	649524.0
46	655322.0
47	657710.5
48	656652.5
49	652468.0
50	644849.0
51	630735.0
52	608326.0
53	578249.5
54	543582.0
55	508035.5
56	469065.5
57	426347.5
58	381373.0
59	332416.0
60	281733.0
61	231387.0
62	186583.0
63	147440.5
64	111414.5
65	83902.5
66	63650.0
67	48712.0
68	37693.5
69	27975.5
70	20138.5
71	14453.0
72	10100.5
73	6895.5
74	4695.0
75	3173.0
76	2258.5
77	1572.0
78	1089.5
79	776.0
80	521.0
81	345.0
82	252.5
83	181.0
84	135.0
85	102.0
86	77.5
87	58.0
88	36.0
89	26.5
90	22.0
91	15.5
92	11.5
93	6.0
94	2.0
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001243092688283354
3	0.0
4	2.889350572766715E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.003094292880835052
18-19	0.0
20-21	0.002096555590994662
22-23	0.0
24-25	3.159426935075894E-4
26-27	0.0032885134947244743
28-29	0.0
30-31	1.4493618257644625E-4
32-33	7.945249320740349E-4
34-35	7.571642765005035E-4
36-37	0.010177591104306912
38-39	0.011751795727506521
40-41	0.025218728324118162
42-43	0.0019070218479611703
44-45	0.0012353414455274943
46-47	0.001648787663027488
48-49	0.0016343111449819354
50-51	4.814278290245358E-5
52-53	1.0870601754530116E-4
54-55	0.00629975732090778
56-57	0.0037697389642140718
58-59	0.003976820705174832
60-61	0.001631729030257116
62-63	4.889066088592042E-4
64-65	7.762740099315928E-4
66-67	6.314415029496365E-4
68-69	2.096826587157239E-5
70-71	4.262061548942276E-6
72-73	1.6024611724740654E-4
74-75	2.816313602152227E-4
76-77	0.0017398736780146833
78-79	0.002215293906954654
80-81	0.011717495897950151
82-83	0.010164472484867317
84-85	0.01319141730950215
86-87	0.006926761675812586
88-89	0.09191222796428289
90-91	4.436441172560581E-4
92-93	3.654541229516101E-5
94-95	2.2510362001994418E-4
96-97	0.015164724531363957
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	436.0
20-21	1435.0
22-23	2743.0
24-25	5831.0
26-27	8943.0
28-29	19189.0
30-31	42067.0
32-33	57417.0
34-35	82013.0
36-37	130588.0
38-39	140258.0
40-41	142788.0
42-43	159227.0
44-45	183464.0
46-47	185542.0
48-49	176495.0
50-51	166808.0
52-53	152115.0
54-55	169288.0
56-57	173634.0
58-59	180272.0
60-61	182639.0
62-63	172022.0
64-65	183371.0
66-67	192863.0
68-69	191757.0
70-71	188846.0
72-73	182334.0
74-75	183703.0
76-77	183601.0
78-79	195125.0
80-81	197090.0
82-83	189301.0
84-85	189231.0
86-87	198820.0
88-89	210709.0
90-91	225291.0
92-93	239542.0
94-95	299571.0
96-97	384197.0
98-99	1199874.0
100-101	7511797.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.70814888631276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.55170391503091	77.6101892105878
2	6.646359476783431	11.392943351762204
3	1.5466185935227814	3.9767345005197052
4	0.5625612856983231	1.9286434572922961
5	0.26226461760301617	1.1239107446565595
6	0.13682781662122406	0.7036355327256584
7	0.07866095282574256	0.4719319259429578
8	0.054730294108224754	0.37526657568155314
9	0.033259353989148636	0.2565537897208079
>10	0.12042037814507778	1.6448293156091265
>50	0.005094184034047612	0.2943632945619661
>100	0.001456770824910689	0.19832306401266214
>500	4.236081317904585E-5	0.022675236926680872
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.719419936666779E-6
2	0.0	2.015825981000034E-5	0.0	6.719419936666779E-6	6.719419936666779E-6
3	0.0	2.6877679746667117E-5	0.0	6.719419936666779E-6	6.719419936666779E-6
4	0.0	6.0474779430001014E-5	0.0	1.3438839873333558E-5	6.719419936666779E-6
5	0.0	6.71941993666678E-5	0.0	1.3438839873333558E-5	6.719419936666779E-6
6	4.703593955666745E-5	6.71941993666678E-5	0.0	1.3438839873333558E-5	6.719419936666779E-6
7	4.703593955666745E-5	6.71941993666678E-5	0.0	1.3438839873333558E-5	2.015825981000034E-5
8	4.703593955666745E-5	8.063303924000136E-5	0.0	1.3438839873333558E-5	2.6877679746667117E-5
9	4.703593955666745E-5	1.612660784800027E-4	0.0	3.35970996833339E-5	4.703593955666745E-5
10-11	5.039564952500084E-5	1.612660784800027E-4	0.0	3.35970996833339E-5	4.703593955666745E-5
12-13	5.375535949333423E-5	1.612660784800027E-4	0.0	3.695680965166729E-5	4.703593955666745E-5
14-15	5.375535949333423E-5	1.9822288813167E-4	0.0	6.0474779430001014E-5	5.375535949333423E-5
16-17	9.40718791133349E-5	2.0158259810000337E-4	0.0	6.71941993666678E-5	6.0474779430001014E-5
18-19	9.40718791133349E-5	2.2846027784667047E-4	0.0	9.071216914500152E-5	6.71941993666678E-5
20-21	9.40718791133349E-5	2.754962174033379E-4	0.0	1.4110781867000236E-4	7.391361930333458E-5
22-23	9.40718791133349E-5	3.594889666116727E-4	0.0	1.948631781633366E-4	8.399274920833474E-5
24-25	9.40718791133349E-5	3.897263563266732E-4	0.0	3.1245302705500523E-4	8.735245917666814E-5
26-27	9.40718791133349E-5	4.1324432610500693E-4	0.0	5.711506946166762E-4	8.735245917666814E-5
28-29	9.40718791133349E-5	4.1660403607334033E-4	0.0	0.002069581340493368	9.74315890816683E-5
30-31	9.40718791133349E-5	4.837982354400081E-4	0.0	0.00635321155011844	1.0079129905000169E-4
32-33	9.40718791133349E-5	4.837982354400081E-4	0.0	0.014930551099273584	1.0079129905000169E-4
34-35	9.40718791133349E-5	5.913089544266766E-4	0.0	0.027136377414228786	1.0751071898666847E-4
36-37	9.40718791133349E-5	6.988196734133451E-4	0.0	0.04407603507456574	1.0751071898666847E-4
38-39	9.40718791133349E-5	7.122585132866786E-4	0.0	0.07258317415587455	1.0751071898666847E-4
40-41	9.74315890816683E-5	7.794527126533464E-4	0.0	0.11877582651049032	1.1423013892333525E-4
42-43	1.0079129905000169E-4	8.096901023683469E-4	0.0	0.14070129376383403	1.1423013892333525E-4
44-45	1.0079129905000169E-4	8.903231416083483E-4	0.0	0.1737306024625196	1.1423013892333525E-4
46-47	1.0079129905000169E-4	9.407187911333492E-4	0.0	0.21208505146101356	1.1423013892333525E-4
48-49	1.0079129905000169E-4	9.440785011016825E-4	0.0	0.2425475417438924	1.1758984889166863E-4
50-51	1.0415100901833507E-4	0.0010717474798983514	0.0	0.274972102648278	1.2430926882833543E-4
52-53	1.0751071898666847E-4	0.0010885460297400183	0.0	0.30999707906815355	1.3438839873333556E-4
54-55	1.0751071898666847E-4	0.001095265449676685	0.0	0.4027788295536484	1.4110781867000236E-4
56-57	1.0751071898666847E-4	0.0011154237094866853	0.0	0.47407523479165126	1.5118694857500255E-4
58-59	1.0751071898666847E-4	0.0011154237094866853	0.0	0.5494469682212426	1.5454665854333592E-4
60-61	1.0751071898666847E-4	0.001138941679265019	0.0	0.5904488686747832	1.612660784800027E-4
62-63	1.2094955886000203E-4	0.001145661099201686	0.0	0.6108255096327253	1.6798549841666948E-4
64-65	1.2094955886000203E-4	0.0012296538484100207	0.0	0.6314574885482606	1.6798549841666948E-4
66-67	1.2094955886000203E-4	0.001239732978315021	0.0	0.6424874163742991	1.6798549841666948E-4
68-69	1.2094955886000203E-4	0.0012766897879666881	0.0	0.6518240503762975	1.6798549841666948E-4
70-71	1.2094955886000203E-4	0.0012901286278400217	0.0	0.6606802458528244	1.7470491835333627E-4
72-73	1.276689787966688E-4	0.001293488337808355	0.0	0.6684714132693895	1.7470491835333627E-4
74-75	1.276689787966688E-4	0.0013338048574283558	0.0	0.6754932071032063	1.847840482583364E-4
76-77	1.276689787966688E-4	0.0013942796368583569	0.0	0.6817825841639265	1.881437582266698E-4
78-79	1.276689787966688E-4	0.0014245170265733572	0.0	0.6874571343004415	1.881437582266698E-4
80-81	1.276689787966688E-4	0.0014278767365416907	0.0	0.6906488587703583	1.881437582266698E-4
82-83	1.276689787966688E-4	0.0014345961564783574	0.0	0.6927553969205033	1.948631781633366E-4
84-85	1.310286887650022E-4	0.001441315576415024	0.0	0.6945897985632133	1.948631781633366E-4
86-87	1.343883987333356E-4	0.001474912676098358	0.0	0.6948384171008699	1.948631781633366E-4
88-89	1.343883987333356E-4	0.001518588905686692	0.0	0.6949224098500784	2.0158259810000337E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13155	0.0	42.78157	1
GTATCAA	22565	0.0	32.25427	1
TCAACGC	26650	0.0	26.27537	4
ATCAACG	27505	0.0	25.458593	3
CAACGCA	27555	0.0	25.456543	5
AACGCAG	28475	0.0	24.7195	6
TATCAAC	29510	0.0	24.1413	2
GTACATG	37855	0.0	22.257704	1
TACATGG	38615	0.0	21.431131	2
ACATGGG	38585	0.0	20.953682	3
ACGCAGA	34355	0.0	20.417845	7
CGCAGAG	35565	0.0	19.757385	8
CATGGGG	27590	0.0	18.781612	4
AGAGTAC	37050	0.0	16.563898	10-11
GCAGAGT	42115	0.0	16.530546	9
GAGTACT	22880	0.0	16.002949	12-13
CAGAGTA	41030	0.0	15.184461	10-11
ATGGGGG	17020	0.0	14.865475	5
GAGTACA	27085	0.0	14.476237	1
GTACTTT	25965	0.0	14.071268	14-15
>>END_MODULE