FastQCFastQC Report
Fri 27 May 2016
SRR2139718_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139718_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7281431
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71050.041.619461
GTATCAA118500.031.2776361
TCAACGC140400.025.5665474
CTTATAC64500.025.046621
CAACGCA144200.024.9486875
ATCAACG144950.024.8195973
AACGCAG149050.024.136876
TATCAAC155500.023.4985642
GTACATG197950.021.9191971
TATACAC86900.021.6963943
TACATGG196850.021.6528322
ACATGGG202500.020.511393
ACGCAGA178450.020.1151187
CGCAGAG181300.019.8211358
CATGGGG135900.018.1127014
TTATACA94550.018.108892
TATGCCG49150.016.8539942-43
ACATCTC95100.016.8178888
ACACATC97900.016.7072436
TACACAT113850.016.5251465
Produced by FastQC (version 0.11.5)