##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139718_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7281431
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13870831159424	33.0	31.0	34.0	30.0	34.0
2	32.263836050908125	34.0	31.0	34.0	30.0	34.0
3	32.318242114771124	34.0	31.0	34.0	30.0	34.0
4	35.950818046617485	37.0	35.0	37.0	35.0	37.0
5	35.81787000384952	37.0	35.0	37.0	35.0	37.0
6	35.83510713759424	37.0	35.0	37.0	35.0	37.0
7	35.74790710781988	37.0	35.0	37.0	33.0	37.0
8	35.709238747163845	37.0	35.0	37.0	33.0	37.0
9	37.3364047534063	39.0	37.0	39.0	34.0	39.0
10-11	37.23460347286131	39.0	37.0	39.0	33.0	39.0
12-13	37.191397405262784	39.0	37.0	39.0	33.0	39.0
14-15	38.3834859521432	40.0	38.0	41.0	33.0	41.0
16-17	38.397932974988024	40.0	38.0	41.0	33.0	41.0
18-19	38.38852685687745	40.0	38.0	41.0	33.5	41.0
20-21	38.35646620578029	40.0	38.0	41.0	34.0	41.0
22-23	38.254146966273495	40.0	38.0	41.0	33.0	41.0
24-25	38.16372052602434	40.0	37.5	41.0	33.0	41.0
26-27	37.992135395021265	40.0	37.0	41.0	33.0	41.0
28-29	37.85839082262271	40.0	37.0	41.0	33.0	41.0
30-31	37.708567173025166	40.0	37.0	41.0	32.0	41.0
32-33	37.906089430735626	40.0	37.0	41.0	33.0	41.0
34-35	37.892935017130874	40.0	37.0	41.0	33.0	41.0
36-37	37.80838150381181	40.0	37.0	41.0	33.0	41.0
38-39	37.694562923472375	40.0	37.0	41.0	33.0	41.0
40-41	37.65680116411052	40.0	37.0	41.0	33.0	41.0
42-43	37.54918679450424	40.0	37.0	41.0	32.0	41.0
44-45	37.59595135585129	39.5	36.5	41.0	32.5	41.0
46-47	37.73235383558159	40.0	37.0	41.0	33.0	41.0
48-49	37.66110163279244	40.0	37.0	41.0	32.0	41.0
50-51	37.54001269902527	40.0	36.5	41.0	32.0	41.0
52-53	37.396906186696214	39.0	36.0	41.0	31.0	41.0
54-55	37.288089982630126	39.0	36.0	41.0	31.0	41.0
56-57	37.11935681507958	39.0	35.5	41.0	31.0	41.0
58-59	36.972110148628545	39.0	35.0	40.0	31.0	41.0
60-61	36.73757580457286	38.5	35.0	40.0	31.0	41.0
62-63	36.47754973263061	38.0	35.0	40.0	30.5	41.0
64-65	36.253467605736546	38.0	35.0	40.0	30.0	41.0
66-67	35.95735065566612	37.0	34.0	40.0	30.0	41.0
68-69	35.633891779394745	37.0	34.0	39.5	29.0	41.0
70-71	35.32681199779354	36.0	34.0	39.0	29.0	40.5
72-73	35.01285081816686	36.0	34.0	39.0	29.0	40.0
74-75	34.622036840213866	35.0	33.5	38.0	29.0	40.0
76-77	33.56631899529391	34.5	32.0	37.0	27.5	39.0
78-79	33.903756964801644	35.0	33.0	37.0	28.5	39.0
80-81	33.77444547491652	35.0	33.0	36.0	29.0	39.0
82-83	33.5414204022923	35.0	33.0	36.0	29.0	37.5
84-85	33.318349322098044	35.0	33.0	35.5	29.0	37.0
86-87	33.101276612736186	35.0	32.0	35.0	28.5	37.0
88-89	32.87512847329846	34.0	32.0	35.0	28.0	36.0
90-91	32.72739934311155	34.0	32.0	35.0	27.0	36.0
92-93	32.63400180004683	34.0	32.0	35.0	27.0	36.0
94-95	32.576195412825875	34.0	32.0	35.0	27.0	35.0
96-97	32.51456208933054	34.0	32.0	35.0	27.0	35.0
98-99	32.641213278428346	34.0	32.0	35.0	28.5	35.0
100-101	31.727262442649632	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	18.0
20	61.0
21	322.0
22	1470.0
23	4783.0
24	12016.0
25	25012.0
26	45226.0
27	71440.0
28	101671.0
29	135916.0
30	172462.0
31	216442.0
32	269235.0
33	341004.0
34	449854.0
35	612892.0
36	917472.0
37	1435442.0
38	1583183.0
39	851734.0
40	33775.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.263065185950396	21.46094634420075	13.952710669097874	25.323277800750976
2	14.611355029989683	22.729836567102993	39.41583805281915	23.24297035008818
3	17.30507368675196	26.920051841458086	31.316454142049828	24.45842032974013
4	12.27460234688094	18.84393528779938	38.40131765706335	30.48014470825633
5	12.674816255211372	38.31205706680459	36.23705285403378	12.776073823950265
6	29.930449110895925	38.603661835153005	18.510262611840997	12.955626442110074
7	26.058105886054538	32.64234736276427	23.69852848979823	17.60101826138296
8	24.582681618489552	36.784610058105336	21.536535881477143	17.09617244192797
9	25.683220784485904	15.895941333509855	20.951966721925952	37.46887116007829
10-11	24.064081634502887	26.75546194147826	29.46845201169935	19.7120044123195
12-13	24.751947797074504	24.296906748137832	29.307357303804704	21.64378815098296
14-15	21.88766878378714	25.753694569103242	26.457608401425485	25.901028245684127
16-17	21.806931366174794	28.783832855250303	27.274163107482018	22.135072671092885
18-19	21.86125227307654	28.11593627681152	28.630162120605142	21.392649329506796
20-21	22.526477713053524	27.42070248990937	28.36229099817845	21.690528798858654
22-23	22.199971704190663	27.523210118361646	28.240934695744503	22.035883481703188
24-25	22.090618554108975	27.638599924131434	28.336317286120245	21.934464235639346
26-27	22.13104608177839	27.69974751169778	28.305033525473334	21.864172881050496
28-29	22.036640202624515	27.644200464070444	28.411098982551312	21.90806035075373
30-31	22.279793995539478	27.64746811159064	28.20334200905953	21.869395883810352
32-33	22.059139034306195	27.693514073185266	28.23280992318188	22.014536969326663
34-35	22.068028814892994	27.82432116331594	28.349736865145548	21.757913156645518
36-37	22.182368839040812	27.701930722658215	28.178255449674538	21.937444988626435
38-39	21.93056879830308	27.71392784998164	28.34627580994432	22.009227541770958
40-41	22.135752174227186	27.5955618255256	28.27498530876975	21.99370069147746
42-43	22.146740501120803	27.677630287575628	28.098728217668672	22.07690099363489
44-45	22.12212317369939	27.812966740721436	27.945026574287525	22.119883511291654
46-47	22.289471236896784	27.68764180426031	27.687826764328545	22.335060194514366
48-49	22.176278003635176	27.761321434951082	27.69899964791604	22.363400913497706
50-51	22.08246045584637	27.83060721862554	27.7947227832333	22.292209542294785
52-53	22.26637733226973	27.779805007215653	27.76720282522906	22.186614835285557
54-55	22.19426773574509	27.719366523848166	27.69356638213457	22.39279935827217
56-57	22.33762579999158	27.91900602460159	27.475491925793317	22.267876249613515
58-59	22.148304851985547	28.024528320676083	27.658069954349106	22.16909687298926
60-61	22.32193975550417	28.076859252421578	27.440002381108673	22.161198610965577
62-63	22.230911580053107	28.223601811860817	27.418808686281693	22.12667792180439
64-65	22.202336106751567	28.089044705177457	27.443374515020263	22.265244673050717
66-67	22.29747888517519	28.008148467616216	27.375103216686124	22.31926943052247
68-69	22.285591828956115	27.92826864480182	27.45675798488764	22.329381541354426
70-71	22.285656034679153	27.73707713846636	27.529753426119306	22.447513400735183
72-73	22.28202422250318	27.807600978264812	27.45024023934525	22.460134559886765
74-75	22.15256844221934	27.890898155540462	27.47146441343899	22.485068988801203
76-77	22.29416354705815	27.840412011743528	27.403649823762706	22.461774617435612
78-79	22.23044742028779	27.856190599305787	27.42503244270185	22.488329537704583
80-81	22.260935571796836	27.832383880673994	27.40188255757228	22.50479798995689
82-83	22.22562665652506	27.86553049374834	27.481446430509294	22.427396419217306
84-85	22.29095982865993	27.881229219099453	27.40316462379721	22.424646328443405
86-87	22.183326712749178	27.859864234428294	27.507827598771918	22.448981454050614
88-89	22.222077310032283	27.865547985963808	27.443646495821405	22.468728208182508
90-91	22.240730664404936	27.909439224495454	27.50181787939468	22.348012231704935
92-93	22.231837381597877	27.830398540303424	27.521917785844174	22.415846292254525
94-95	22.048615813927878	27.916657970310414	27.603167279556253	22.431558936205455
96-97	21.985164104388698	27.95831869040462	27.651291084113616	22.405226121093065
98-99	22.119923425765077	28.567611370225453	27.73676684514249	21.575698358866976
100-101	24.97196812650756	36.32670715072864	28.899368464045992	9.801956258717816
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1863.0
1	1711.0
2	2153.0
3	2881.5
4	3752.0
5	5535.5
6	6891.0
7	7779.0
8	8271.5
9	8185.0
10	7784.5
11	7364.5
12	8155.0
13	10837.0
14	16721.0
15	24277.5
16	29886.5
17	32572.0
18	33107.0
19	32611.5
20	31578.0
21	30907.0
22	31996.5
23	36077.0
24	43655.0
25	54434.5
26	68389.5
27	83849.5
28	100325.5
29	118288.0
30	135319.5
31	151956.0
32	169522.5
33	186913.0
34	202831.0
35	217879.5
36	235230.5
37	251399.0
38	263641.0
39	275078.5
40	286720.0
41	297837.0
42	305263.5
43	311986.0
44	318774.0
45	323628.5
46	330273.0
47	336622.5
48	335926.5
49	330328.5
50	323689.5
51	313294.5
52	298718.0
53	281424.0
54	263590.0
55	244452.0
56	224249.5
57	203404.5
58	180674.5
59	156167.0
60	131726.0
61	108756.0
62	87579.0
63	68419.5
64	51571.5
65	38797.5
66	29324.5
67	22354.5
68	16934.5
69	12389.0
70	9049.0
71	6538.5
72	4629.5
73	3261.5
74	2314.0
75	1655.5
76	1199.5
77	829.5
78	555.0
79	368.0
80	240.0
81	161.5
82	121.0
83	86.5
84	61.5
85	47.5
86	39.0
87	28.5
88	18.5
89	14.5
90	10.0
91	6.0
92	4.5
93	3.5
94	2.0
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0012909550334268085
3	0.0
4	3.021384120786148E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0036943287658703352
18-19	0.0
20-21	0.0023348256760779597
22-23	0.0
24-25	3.7783005877180915E-4
26-27	0.0037524414064177883
28-29	0.0
30-31	1.1712457783479371E-4
32-33	8.777952161819544E-4
34-35	9.37316051724849E-4
36-37	0.009788164542961232
38-39	0.011145962341612099
40-41	0.025257043181771344
42-43	0.0018951137410518025
44-45	0.0012110080043981476
46-47	0.0017459925586684943
48-49	0.0017630145357922708
50-51	2.2807898648996932E-5
52-53	1.2317328248329654E-4
54-55	0.006917515914575931
56-57	0.004177203292775432
58-59	0.004448925134869085
60-61	0.0019244206617218297
62-63	5.712534816450875E-4
64-65	9.2462297027396E-4
66-67	7.519817924699449E-4
68-69	0.0
70-71	0.0
72-73	1.1669934397912626E-4
74-75	2.553901225498624E-4
76-77	0.001511072546314837
78-79	0.0019989000392519854
80-81	0.011510586379414656
82-83	0.009971152088418703
84-85	0.013165869051848446
86-87	0.006782343643408094
88-89	0.08564215169985347
90-91	4.649691699021652E-4
92-93	5.408524223590123E-5
94-95	1.7762738659046453E-4
96-97	0.015070514962482802
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	231.0
20-21	711.0
22-23	1389.0
24-25	2884.0
26-27	4527.0
28-29	9536.0
30-31	21552.0
32-33	29499.0
34-35	43377.0
36-37	66420.0
38-39	71997.0
40-41	71060.0
42-43	81712.0
44-45	94017.0
46-47	95037.0
48-49	89695.0
50-51	82904.0
52-53	81739.0
54-55	89591.0
56-57	93881.0
58-59	94351.0
60-61	93875.0
62-63	90144.0
64-65	94081.0
66-67	100207.0
68-69	93668.0
70-71	91442.0
72-73	88070.0
74-75	88336.0
76-77	89113.0
78-79	94370.0
80-81	94536.0
82-83	90398.0
84-85	91558.0
86-87	95874.0
88-89	101654.0
90-91	108064.0
92-93	114449.0
94-95	142139.0
96-97	184114.0
98-99	573879.0
100-101	3635350.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.64729079584868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.98898993091449	81.54574740422173
2	5.851038648939812	10.373574491406343
3	1.2676438935925523	3.3711959058264256
4	0.4305260477072035	1.5265987098515157
5	0.17577968364028165	0.7791196365831166
6	0.10529636487324698	0.5600542485998705
7	0.053391628030329874	0.33131162232479067
8	0.0330522899429673	0.23439967664343414
9	0.024137499517339334	0.19257515449184134
>10	0.06837385318810253	0.951651218394425
>50	0.001633927015755712	0.0941405762571032
>100	9.187454952034208E-5	0.018908537164183253
>500	4.43580885033938E-5	0.02072281823518065
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.373356418539158E-5	0.0	0.0	0.0
3	0.0	1.373356418539158E-5	0.0	1.373356418539158E-5	0.0
4	1.373356418539158E-5	5.493425674156632E-5	0.0	1.373356418539158E-5	0.0
5	2.746712837078316E-5	5.493425674156632E-5	0.0	2.746712837078316E-5	0.0
6	9.613494929774107E-5	5.493425674156632E-5	0.0	2.746712837078316E-5	0.0
7	1.0986851348313264E-4	5.493425674156632E-5	0.0	4.120069255617474E-5	0.0
8	1.2360207766852423E-4	5.493425674156632E-5	0.0	4.120069255617474E-5	1.373356418539158E-5
9	1.2360207766852423E-4	1.5106920603930737E-4	0.0	5.493425674156632E-5	1.373356418539158E-5
10-11	1.2360207766852423E-4	1.5106920603930737E-4	0.0	6.180103883426211E-5	1.373356418539158E-5
12-13	1.2360207766852423E-4	1.5106920603930737E-4	0.0	6.866782092695791E-5	1.373356418539158E-5
14-15	1.2360207766852423E-4	2.060034627808737E-4	0.0	6.866782092695791E-5	2.060034627808737E-5
16-17	1.7166955231739475E-4	2.060034627808737E-4	0.0	9.613494929774107E-5	2.746712837078316E-5
18-19	1.7853633441009055E-4	2.3347059115165687E-4	0.0	1.0986851348313264E-4	3.4333910463478953E-5
20-21	1.7853633441009055E-4	2.6093771952244004E-4	0.0	1.3733564185391581E-4	4.120069255617474E-5
22-23	1.7853633441009055E-4	3.433391046347895E-4	0.0	1.7853633441009055E-4	4.120069255617474E-5
24-25	2.060034627808737E-4	4.3947405393253056E-4	0.0	2.1973702696626528E-4	4.120069255617474E-5
26-27	2.3347059115165687E-4	4.6694118230331374E-4	0.0	3.296055404493979E-4	4.120069255617474E-5
28-29	2.3347059115165687E-4	4.6694118230331374E-4	0.0	8.720813257723653E-4	4.120069255617474E-5
30-31	2.3347059115165687E-4	5.493425674156633E-4	0.0	0.002581910066853617	4.120069255617474E-5
32-33	2.4033737324435266E-4	5.493425674156633E-4	0.0	0.006049635023664991	4.120069255617474E-5
34-35	2.4720415533704845E-4	5.905432599718379E-4	0.0	0.010856382488552044	4.120069255617474E-5
36-37	2.4720415533704845E-4	6.386107346207084E-4	0.0	0.017359225130334957	4.120069255617474E-5
38-39	2.4720415533704845E-4	6.729446450841874E-4	0.0	0.02619677368363444	4.120069255617474E-5
40-41	2.7467128370783163E-4	7.004117734549706E-4	0.0	0.035075522929490094	4.806747464887053E-5
42-43	2.7467128370783163E-4	7.072785555476665E-4	0.0	0.0428967877330706	5.493425674156632E-5
44-45	2.8153806580052737E-4	7.622128122892327E-4	0.0	0.05299782419142611	5.493425674156632E-5
46-47	2.8840484789322316E-4	8.102802869381032E-4	0.0	0.06401900945020285	5.493425674156632E-5
48-49	2.8840484789322316E-4	8.171470690307989E-4	0.0	0.07427111511459766	5.493425674156632E-5
50-51	2.8840484789322316E-4	8.789481078650611E-4	0.0	0.08411121385343073	5.493425674156632E-5
52-53	2.9527162998591895E-4	8.789481078650611E-4	0.0	0.09312043195904761	5.493425674156632E-5
54-55	3.0213841207861475E-4	8.995484541431484E-4	0.0	0.10280259470974867	5.493425674156632E-5
56-57	3.0213841207861475E-4	9.201488004212359E-4	0.0	0.11327443740110975	5.493425674156632E-5
58-59	3.0213841207861475E-4	9.201488004212359E-4	0.0	0.12340294098783604	5.493425674156632E-5
60-61	3.0213841207861475E-4	9.270155825139316E-4	0.0	0.13270743072343885	5.493425674156632E-5
62-63	3.1587197626400633E-4	9.476159287920191E-4	0.0	0.14166858135440685	5.493425674156632E-5
64-65	3.1587197626400633E-4	0.001016283749718977	0.0	0.1508769361407119	5.493425674156632E-5
66-67	3.1587197626400633E-4	0.001016283749718977	0.0	0.16077883591837921	5.493425674156632E-5
68-69	3.1587197626400633E-4	0.0010300173139043685	0.0	0.17055713361837804	5.493425674156632E-5
70-71	3.1587197626400633E-4	0.0010300173139043685	0.0	0.17988222370025891	5.493425674156632E-5
72-73	3.296055404493979E-4	0.0010368840959970643	0.0	0.18732581548874117	5.493425674156632E-5
74-75	3.296055404493979E-4	0.0010643512243678475	0.0	0.19402779481121224	5.493425674156632E-5
76-77	3.296055404493979E-4	0.0011261522632021095	0.0	0.2004276357216047	5.493425674156632E-5
78-79	3.296055404493979E-4	0.0011536193915728926	0.0	0.20596226208831753	5.493425674156632E-5
80-81	3.296055404493979E-4	0.0011536193915728926	0.0	0.2089493122986402	5.493425674156632E-5
82-83	3.296055404493979E-4	0.0011604861736655885	0.0	0.21083081059203884	5.493425674156632E-5
84-85	3.433391046347895E-4	0.0012154204304071548	0.0	0.21229343517778304	5.493425674156632E-5
86-87	3.433391046347895E-4	0.0012360207766852422	0.0	0.21262990750032515	5.493425674156632E-5
88-89	3.433391046347895E-4	0.0012909550334268085	0.0	0.21266424141078863	5.493425674156632E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7105	0.0	41.61946	1
GTATCAA	11850	0.0	31.277636	1
TCAACGC	14040	0.0	25.566547	4
CTTATAC	6450	0.0	25.04662	1
CAACGCA	14420	0.0	24.948687	5
ATCAACG	14495	0.0	24.819597	3
AACGCAG	14905	0.0	24.13687	6
TATCAAC	15550	0.0	23.498564	2
GTACATG	19795	0.0	21.919197	1
TATACAC	8690	0.0	21.696394	3
TACATGG	19685	0.0	21.652832	2
ACATGGG	20250	0.0	20.51139	3
ACGCAGA	17845	0.0	20.115118	7
CGCAGAG	18130	0.0	19.821135	8
CATGGGG	13590	0.0	18.112701	4
TTATACA	9455	0.0	18.10889	2
TATGCCG	4915	0.0	16.85399	42-43
ACATCTC	9510	0.0	16.817888	8
ACACATC	9790	0.0	16.707243	6
TACACAT	11385	0.0	16.525146	5
>>END_MODULE