FastQCFastQC Report
Fri 27 May 2016
SRR2139717_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139717_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9654798
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87700.042.5461351
GTATCAA147300.031.8497411
TCAACGC168800.026.736874
ATCAACG174400.025.878353
CAACGCA174500.025.8633825
AACGCAG180100.025.1491746
TATCAAC191550.023.9422172
GTACATG227250.022.3916381
TACATGG231250.021.5838572
ACGCAGA217500.020.8060447
ACATGGG235500.020.6609333
CGCAGAG223000.020.365568
CATGGGG169550.018.2554864
AGAGTAC237350.016.21615610-11
GCAGAGT279750.016.0749079
GAGTACT155300.016.04416812-13
CAGAGTA265750.015.27594410-11
GAGTACA163050.014.7841471
ATGGGGG99750.014.1338975
GTACTTT179150.013.88647914-15
Produced by FastQC (version 0.11.5)