##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139717_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9654798
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16187899529332	33.0	31.0	34.0	30.0	34.0
2	32.291821019973696	34.0	31.0	34.0	30.0	34.0
3	32.34721006073871	34.0	31.0	34.0	30.0	34.0
4	35.97260709131356	37.0	35.0	37.0	35.0	37.0
5	35.83977417238558	37.0	35.0	37.0	35.0	37.0
6	35.85591992706632	37.0	35.0	37.0	35.0	37.0
7	35.76974681396752	37.0	35.0	37.0	33.0	37.0
8	35.7295602663049	37.0	35.0	37.0	33.0	37.0
9	37.36379590748558	39.0	37.0	39.0	34.0	39.0
10-11	37.263170653596276	39.0	37.0	39.0	33.5	39.0
12-13	37.22450899542383	39.0	37.0	39.0	33.0	39.0
14-15	38.42578746857262	40.0	38.0	41.0	33.0	41.0
16-17	38.443357748137245	40.0	38.0	41.0	33.0	41.0
18-19	38.43647754204697	40.0	38.0	41.0	33.5	41.0
20-21	38.40450344652953	40.0	38.0	41.0	34.0	41.0
22-23	38.304906026513116	40.0	38.0	41.0	33.5	41.0
24-25	38.218798619231855	40.0	38.0	41.0	33.0	41.0
26-27	38.05635894359165	40.0	37.0	41.0	33.0	41.0
28-29	37.92205788507883	40.0	37.0	41.0	33.0	41.0
30-31	37.774669564893614	40.0	37.0	41.0	33.0	41.0
32-33	37.96973299571008	40.0	37.0	41.0	33.0	41.0
34-35	37.95610809853924	40.0	37.0	41.0	33.0	41.0
36-37	37.87672201779118	40.0	37.0	41.0	33.0	41.0
38-39	37.77208294861221	40.0	37.0	41.0	33.0	41.0
40-41	37.7248345153545	40.0	37.0	41.0	33.0	41.0
42-43	37.620050722619105	40.0	37.0	41.0	32.0	41.0
44-45	37.66243163695087	40.0	37.0	41.0	32.5	41.0
46-47	37.79731588521664	40.0	37.0	41.0	33.0	41.0
48-49	37.72548080955207	40.0	37.0	41.0	32.5	41.0
50-51	37.607650611296364	40.0	36.5	41.0	32.0	41.0
52-53	37.45993389437286	39.5	36.0	41.0	32.0	41.0
54-55	37.358723643468174	39.0	36.0	41.0	31.5	41.0
56-57	37.19443255753157	39.0	36.0	41.0	31.0	41.0
58-59	37.04895361302354	39.0	35.0	40.5	31.0	41.0
60-61	36.82174274892993	38.5	35.0	40.0	31.0	41.0
62-63	36.55867633582659	38.0	35.0	40.0	31.0	41.0
64-65	36.33305425218131	38.0	35.0	40.0	30.0	41.0
66-67	36.04156237924143	37.0	34.0	40.0	30.0	41.0
68-69	35.71861149984455	37.0	34.0	39.5	29.5	41.0
70-71	35.414724273179004	36.0	34.0	39.0	29.0	41.0
72-73	35.10306140477661	36.0	34.0	39.0	29.0	40.0
74-75	34.71344637224752	35.0	33.5	38.0	29.0	40.0
76-77	33.66009936150044	35.0	32.0	37.0	27.5	39.0
78-79	33.98512196091639	35.0	33.0	37.0	29.0	39.0
80-81	33.85832698089005	35.0	33.0	36.5	29.0	39.0
82-83	33.62305097494869	35.0	33.0	36.0	29.0	37.5
84-85	33.396348555596646	35.0	33.0	36.0	29.0	37.0
86-87	33.1795360074522	35.0	32.5	35.0	29.0	37.0
88-89	32.95115177144721	34.0	32.0	35.0	28.0	36.0
90-91	32.80634586916331	34.0	32.0	35.0	28.0	36.0
92-93	32.710102637395316	34.0	32.0	35.0	27.5	36.0
94-95	32.652815408021816	34.0	32.0	35.0	28.0	35.0
96-97	32.58595944221376	34.0	32.0	35.0	27.5	35.0
98-99	32.706908224217884	34.0	32.0	35.0	29.0	35.0
100-101	31.795257095796075	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	49.0
21	371.0
22	1750.0
23	5884.0
24	15426.0
25	31903.0
26	57533.0
27	91151.0
28	130638.0
29	175659.0
30	224409.0
31	280265.0
32	348755.0
33	443443.0
34	584373.0
35	800476.0
36	1200252.0
37	1892789.0
38	2144298.0
39	1177068.0
40	48299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.37311790469361	21.446072719491386	13.932440637287286	25.248368738527727
2	14.573255102525936	22.71988535299827	39.45565668211237	23.25120286236342
3	17.21490185501551	26.99417429551607	31.298718005286077	24.492205844182344
4	12.273475984049751	18.69727412488603	38.73531542073862	30.293934470325606
5	12.639249417750637	38.263006641878995	36.36655060002291	12.731193340347463
6	29.94898495028068	38.736750370126856	18.548611788667145	12.765652890925319
7	25.984551929517323	32.69609576502791	24.022035468789717	17.297316836665043
8	24.47890675703417	36.86125799835481	21.639997025313217	17.019838219297803
9	25.629288152895587	15.967604915193462	21.18168603838216	37.221420893528794
10-11	23.919086655153222	26.81295351803321	29.694992065085152	19.572967761728417
12-13	24.60653242046079	24.42130327325336	29.49414891953203	21.478015386753817
14-15	21.761863893993432	25.858821696735657	26.673162918582033	25.706151490688878
16-17	21.698614489503953	28.8534549513325	27.495159766044658	21.95277079311889
18-19	21.73173897579214	28.27030146047592	28.783253673458525	21.214705890273418
20-21	22.375388945447757	27.58163730528515	28.510305335545095	21.532668413721996
22-23	22.095952729094055	27.596074708169592	28.391854801909606	21.916117760826744
24-25	21.952526458233603	27.675581749569478	28.474670139860287	21.897221652336636
26-27	21.97509751237897	27.811245203679423	28.405786044595644	21.807871239345967
28-29	21.92152366047203	27.825824164625	28.426293501391587	21.826358673511383
30-31	22.18953770486949	27.730346931084167	28.295541409214835	21.784573954831508
32-33	21.865239127964372	27.933819154590562	28.296134804743613	21.904806912701453
34-35	22.052970314723076	27.896906479574916	28.32809634749215	21.72202685820986
36-37	21.987651358569178	27.83852943647767	28.30076193240619	21.873057272546966
38-39	21.859613355592725	27.87340791243344	28.408577514476807	21.858401217497022
40-41	22.09008876675215	27.750704682532344	28.298661052184894	21.860545498530612
42-43	22.001908089962043	27.845762963005534	28.128718055121	22.023610891911428
44-45	22.0255535749347	27.918722605426915	28.005143213919503	22.05058060571888
46-47	22.152183282914898	27.859491521267298	27.800297289059866	22.188027906757934
48-49	22.053870507275565	27.920668576878803	27.807992399881392	22.217468515964235
50-51	22.04712543177591	28.02329619089606	27.782350519809363	22.147227857518672
52-53	22.213817186072475	27.915800258437528	27.73329695516587	22.13708560032412
54-55	22.136006915534463	27.847332741745845	27.721680869994085	22.29497947272561
56-57	22.168382747985806	27.985603502080508	27.64497642259856	22.20103732733513
58-59	22.116991955353754	28.074335680972755	27.66634222880623	22.14233013486726
60-61	22.192726977089343	28.048795162435024	27.62929471357235	22.12918314690328
62-63	22.167315201376425	28.13969190424087	27.589608978832477	22.10338391555023
64-65	22.16645211548969	28.049076436850207	27.559301385918268	22.22517006174184
66-67	22.182020556515095	28.04960538559042	27.49055341006703	22.27782064782745
68-69	22.17765302091847	28.058564683015735	27.53517317193277	22.228609124133026
70-71	22.20050780746562	27.812449424439823	27.616241796252723	22.370800971841838
72-73	22.23082954644559	27.887234809010508	27.541481609195866	22.34045403534804
74-75	22.078462851505236	28.030518623889584	27.507455176732364	22.38356334787282
76-77	22.198718954723937	27.911977262196796	27.526509995789883	22.36279378728938
78-79	22.149306412217832	27.950418750004836	27.47848035047427	22.42179448730306
80-81	22.175189227283397	27.989177946384103	27.449729860517646	22.385902965814854
82-83	22.179882801403945	27.978734444486662	27.533378029175886	22.308004724933504
84-85	22.17674318473276	27.971827692376216	27.532985540813655	22.318443582077364
86-87	22.123731418777158	27.95433977071597	27.556173680044218	22.36575513046266
88-89	22.16229920399346	27.94996287772494	27.491259672243494	22.396478246038104
90-91	22.14290131297794	27.999550983994943	27.583510084151115	22.274037618876
92-93	22.140145810746578	27.94458475733359	27.565151437952718	22.35011799396712
94-95	21.96851042987489	27.94484929322691	27.66866402603567	22.417976250862537
96-97	21.9119735176378	28.03432613679132	27.739359154206728	22.31434119136415
98-99	22.041699030992675	28.644021536544777	27.817005075821427	21.497274356641125
100-101	24.872881825398043	36.41583514624383	28.96906478359859	9.742218244759536
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2334.0
1	2164.0
2	2803.5
3	3800.5
4	4832.5
5	7074.5
6	8934.5
7	10196.0
8	10934.0
9	10768.5
10	10163.5
11	9648.0
12	10723.5
13	14064.0
14	21261.5
15	30681.5
16	37951.0
17	41830.5
18	43178.5
19	42989.5
20	41907.0
21	41612.5
22	43978.5
23	50246.0
24	60647.5
25	75580.0
26	94612.5
27	115393.0
28	138685.5
29	163562.5
30	186435.0
31	209509.5
32	233707.0
33	257400.0
34	279610.5
35	299779.0
36	321054.5
37	339801.0
38	355951.5
39	371122.0
40	385388.0
41	399300.5
42	407407.0
43	413164.5
44	419528.5
45	423731.5
46	426881.5
47	428537.5
48	425473.0
49	418654.0
50	410234.5
51	396807.0
52	378628.0
53	358997.0
54	337644.0
55	314215.0
56	288497.5
57	261973.0
58	234424.0
59	203683.5
60	171461.5
61	140993.5
62	113715.5
63	89125.5
64	67470.5
65	50852.5
66	38484.0
67	29593.5
68	22811.5
69	16704.0
70	12082.0
71	8883.0
72	6330.5
73	4397.0
74	3106.5
75	2201.0
76	1515.0
77	977.0
78	663.0
79	473.0
80	342.5
81	244.0
82	178.5
83	134.5
84	93.5
85	63.0
86	48.0
87	35.0
88	23.0
89	15.0
90	10.0
91	6.0
92	5.0
93	2.5
94	1.0
95	1.0
96	0.5
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0012325477964427636
3	0.0
4	2.796537017139043E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.003169408619424249
18-19	0.0
20-21	0.002211435389367481
22-23	0.0
24-25	3.574724089583829E-4
26-27	0.003208112263820226
28-29	0.0
30-31	1.3506219172379407E-4
32-33	8.597087640170303E-4
34-35	7.901596551596746E-4
36-37	0.009971267158954746
38-39	0.011545877053419881
40-41	0.02455088624727412
42-43	0.0020168072693974707
44-45	0.0013769614706431266
46-47	0.0018906208025785398
48-49	0.0018091435807358785
50-51	7.991747493202876E-5
52-53	1.2133745071749435E-4
54-55	0.006208474245510924
56-57	0.0037857228641995934
58-59	0.0038887686942321374
60-61	0.0015881124166141722
62-63	4.461166935937333E-4
64-65	7.919506139597134E-4
66-67	6.9581777132632E-4
68-69	1.297418118482947E-5
70-71	6.592333274617618E-6
72-73	1.406642446963049E-4
74-75	2.7219776147282946E-4
76-77	0.0016391998104338043
78-79	0.0020893622337857048
80-81	0.011473406727326755
82-83	0.010238203769861221
84-85	0.013553533972645464
86-87	0.006993104798668513
88-89	0.0843299057472959
90-91	4.49013988912663E-4
92-93	3.224758925084658E-5
94-95	2.72934116185572E-4
96-97	0.01435965803316816
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	290.0
20-21	879.0
22-23	1720.0
24-25	3329.0
26-27	5692.0
28-29	11595.0
30-31	26816.0
32-33	37415.0
34-35	54036.0
36-37	84314.0
38-39	91158.0
40-41	90486.0
42-43	103632.0
44-45	120612.0
46-47	122189.0
48-49	114556.0
50-51	106660.0
52-53	106083.0
54-55	116771.0
56-57	120482.0
58-59	120212.0
60-61	118154.0
62-63	113507.0
64-65	119576.0
66-67	126373.0
68-69	122981.0
70-71	121631.0
72-73	116893.0
74-75	118358.0
76-77	119845.0
78-79	125570.0
80-81	126071.0
82-83	120586.0
84-85	121134.0
86-87	128185.0
88-89	134443.0
90-91	143853.0
92-93	151257.0
94-95	188328.0
96-97	242251.0
98-99	762621.0
100-101	4894254.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.86262879874518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.46892571569329	80.36700266777956
2	6.18702634394975	10.872167980530289
3	1.3640019206909029	3.5953438331532075
4	0.46518776210658874	1.6349047865476074
5	0.20328929998188594	0.8930766151532601
6	0.11106409001337957	0.5855029748229594
7	0.06355885921306098	0.3909113917736217
8	0.032130456713405774	0.22584531130752972
9	0.025165206765751038	0.19899730986325687
>10	0.07768124425007641	1.0868238705874482
>50	0.001654400463503777	0.09879030174396221
>100	3.147001583413984E-4	0.05063295673729686
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.21452670475343E-5	0.0	0.0	0.0
3	0.0	6.21452670475343E-5	0.0	0.0	0.0
4	0.0	1.139329895871462E-4	0.0	0.0	0.0
5	0.0	1.242905340950686E-4	0.0	0.0	0.0
6	3.107263352376715E-5	1.242905340950686E-4	0.0	0.0	0.0
7	3.107263352376715E-5	1.242905340950686E-4	0.0	0.0	1.0357544507922383E-5
8	3.107263352376715E-5	1.3464807860299097E-4	0.0	0.0	1.0357544507922383E-5
9	3.107263352376715E-5	2.3822352368221478E-4	0.0	0.0	1.0357544507922383E-5
10-11	3.625140577772834E-5	2.3822352368221478E-4	0.0	0.0	4.143017803168953E-5
12-13	4.143017803168953E-5	2.3822352368221478E-4	0.0	0.0	6.21452670475343E-5
14-15	4.660895028565072E-5	2.900112462218267E-4	0.0	0.0	9.321790057130144E-5
16-17	9.321790057130144E-5	3.107263352376715E-4	0.0	5.178772253961191E-6	1.191117618411074E-4
18-19	1.0357544507922383E-4	3.2626265199955505E-4	0.0	1.0357544507922383E-5	1.3464807860299097E-4
20-21	1.0357544507922383E-4	3.417989687614386E-4	0.0	6.21452670475343E-5	1.4500562311091334E-4
22-23	1.0357544507922383E-4	4.2983809707877887E-4	0.0	1.3982685085695218E-4	1.6572071212675812E-4
24-25	1.1393298958714622E-4	4.660895028565072E-4	0.0	1.9161457339656406E-4	1.8643580114260287E-4
26-27	1.242905340950686E-4	4.7644704736442956E-4	0.0	4.039442358089729E-4	1.8643580114260287E-4
28-29	1.242905340950686E-4	4.7644704736442956E-4	0.0	0.0014707713201249782	1.8643580114260287E-4
30-31	1.3464807860299097E-4	5.489498589198862E-4	0.0	0.004707503978850723	1.8643580114260287E-4
32-33	1.3464807860299097E-4	5.541286311738474E-4	0.0	0.011020427356429415	1.8643580114260287E-4
34-35	1.5018439536487456E-4	6.369889872372264E-4	0.0	0.018835194687656855	1.9679334565052525E-4
36-37	1.5536316761883575E-4	7.04313026538722E-4	0.0	0.029731331509991195	1.9679334565052525E-4
38-39	1.5536316761883575E-4	7.146705710466444E-4	0.0	0.04410242451473351	1.9679334565052525E-4
40-41	1.6054193987279693E-4	7.768158380941786E-4	0.0	0.05890853438880855	2.0715089015844765E-4
42-43	1.6572071212675812E-4	8.027096993639847E-4	0.0	0.0708714982954589	2.1232966241240884E-4
44-45	1.6572071212675812E-4	8.907488276813249E-4	0.0	0.08572939589207355	2.1750843466637003E-4
46-47	1.6572071212675812E-4	9.528940947288591E-4	0.0	0.10079444437884666	2.1750843466637003E-4
48-49	1.6572071212675812E-4	9.528940947288591E-4	0.0	0.11395888344841601	2.1750843466637003E-4
50-51	1.8125702888864169E-4	0.0010409332230461995	0.0	0.12577684173195544	2.1750843466637003E-4
52-53	1.9161457339656406E-4	0.001056469539808083	0.0	0.13633117958552837	2.3304475142825362E-4
54-55	1.9679334565052525E-4	0.0010875421733318501	0.0	0.14775037240551278	2.5375984044409837E-4
56-57	1.9679334565052525E-4	0.001139329895871462	0.0	0.1583202465758476	2.6411738495202075E-4
58-59	1.9679334565052525E-4	0.0011548662126333454	0.0	0.1680977685913263	2.6929615720598193E-4
60-61	2.1750843466637003E-4	0.0011704025293952291	0.0	0.1771036535409648	2.6929615720598193E-4
62-63	2.485810681901372E-4	0.0011911176184110739	0.0	0.186285616747238	2.6929615720598193E-4
64-65	2.485810681901372E-4	0.0012532628854586083	0.0	0.19601652981243106	2.6929615720598193E-4
66-67	2.485810681901372E-4	0.0012584416577125696	0.0	0.20516224161292657	2.6929615720598193E-4
68-69	2.485810681901372E-4	0.0012843355189823755	0.0	0.21552496489310288	2.744749294599431E-4
70-71	2.485810681901372E-4	0.001325765697014065	0.0	0.22459299510978892	3.0036879072974906E-4
72-73	2.485810681901372E-4	0.0013413020137759486	0.0	0.23186399135435043	3.2108387974559387E-4
74-75	2.485810681901372E-4	0.0013516595582838708	0.0	0.23868961318507131	3.2108387974559387E-4
76-77	2.5375984044409837E-4	0.001403447280823483	0.0	0.24519415113604656	3.2108387974559387E-4
78-79	2.5893861269805956E-4	0.0014189835975853664	0.0	0.2507509737645469	3.2108387974559387E-4
80-81	2.5893861269805956E-4	0.0014293411420932887	0.0	0.25383752202790777	3.417989687614386E-4
82-83	2.5893861269805956E-4	0.0014345199143472498	0.0	0.25569152249482585	3.6251405777728337E-4
84-85	2.5893861269805956E-4	0.0014552350033630946	0.0	0.2571778301317128	3.6251405777728337E-4
86-87	2.5893861269805956E-4	0.0014604137756170559	0.0	0.2573746234773633	3.6251405777728337E-4
88-89	2.6929615720598193E-4	0.0015070227259027067	0.0	0.2574264111999029	3.7287160228520575E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8770	0.0	42.546135	1
GTATCAA	14730	0.0	31.849741	1
TCAACGC	16880	0.0	26.73687	4
ATCAACG	17440	0.0	25.87835	3
CAACGCA	17450	0.0	25.863382	5
AACGCAG	18010	0.0	25.149174	6
TATCAAC	19155	0.0	23.942217	2
GTACATG	22725	0.0	22.391638	1
TACATGG	23125	0.0	21.583857	2
ACGCAGA	21750	0.0	20.806044	7
ACATGGG	23550	0.0	20.660933	3
CGCAGAG	22300	0.0	20.36556	8
CATGGGG	16955	0.0	18.255486	4
AGAGTAC	23735	0.0	16.216156	10-11
GCAGAGT	27975	0.0	16.074907	9
GAGTACT	15530	0.0	16.044168	12-13
CAGAGTA	26575	0.0	15.275944	10-11
GAGTACA	16305	0.0	14.784147	1
ATGGGGG	9975	0.0	14.133897	5
GTACTTT	17915	0.0	13.886479	14-15
>>END_MODULE