FastQCFastQC Report
Fri 27 May 2016
SRR2139716_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139716_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10794328
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95900.044.5438161
GTATCAA166900.031.7209361
TCAACGC196150.025.9805134
ATCAACG201350.025.3095513
CAACGCA203300.025.0467935
AACGCAG210500.024.2284836
TATCAAC216750.023.8288332
GTACATG272600.021.422641
TACATGG277050.020.8013942
ACGCAGA250300.020.3920767
ACATGGG276200.020.2211253
CGCAGAG256500.019.9306818
CTTATAC80200.018.1913051
CATGGGG198200.018.1063234
AGAGTAC272150.016.28341110-11
GAGTACT168500.016.09413712-13
TATACAC115650.016.0393963
GCAGAGT313850.015.9539479
CAGAGTA302500.015.12398110-11
TATGCCG48800.014.8954142-43
Produced by FastQC (version 0.11.5)