##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139716_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10794328
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13605274918457	33.0	31.0	34.0	30.0	34.0
2	32.261676873261585	34.0	31.0	34.0	30.0	34.0
3	32.31774103955337	34.0	31.0	34.0	30.0	34.0
4	35.951503882409355	37.0	35.0	37.0	35.0	37.0
5	35.814842109670934	37.0	35.0	37.0	35.0	37.0
6	35.832810064693234	37.0	35.0	37.0	35.0	37.0
7	35.743650924819036	37.0	35.0	37.0	33.0	37.0
8	35.70539379570456	37.0	35.0	37.0	33.0	37.0
9	37.335263482821716	39.0	37.0	39.0	34.0	39.0
10-11	37.2296794205253	39.0	37.0	39.0	33.0	39.0
12-13	37.1896980988534	39.0	37.0	39.0	33.0	39.0
14-15	38.38136361985665	40.0	38.0	41.0	33.0	41.0
16-17	38.393685276193196	40.0	38.0	41.0	33.0	41.0
18-19	38.386099301410894	40.0	38.0	41.0	33.5	41.0
20-21	38.35311645676461	40.0	38.0	41.0	34.0	41.0
22-23	38.249687414784404	40.0	38.0	41.0	33.0	41.0
24-25	38.16065579310079	40.0	37.5	41.0	33.0	41.0
26-27	37.99153392577313	40.0	37.0	41.0	33.0	41.0
28-29	37.85561541987358	40.0	37.0	41.0	33.0	41.0
30-31	37.70335237600631	40.0	37.0	41.0	32.0	41.0
32-33	37.908134399667716	40.0	37.0	41.0	33.0	41.0
34-35	37.89675205390208	40.0	37.0	41.0	33.0	41.0
36-37	37.8125612004324	40.0	37.0	41.0	33.0	41.0
38-39	37.704766161901006	40.0	37.0	41.0	33.0	41.0
40-41	37.660395757763226	40.0	37.0	41.0	33.0	41.0
42-43	37.55307565623343	40.0	37.0	41.0	32.0	41.0
44-45	37.595698390405964	39.5	36.5	41.0	32.5	41.0
46-47	37.730420242160726	40.0	37.0	41.0	32.5	41.0
48-49	37.65812631261153	40.0	37.0	41.0	32.0	41.0
50-51	37.53439937231917	40.0	36.5	41.0	32.0	41.0
52-53	37.386523255856545	39.0	36.0	41.0	31.0	41.0
54-55	37.27379069582353	39.0	36.0	41.0	31.0	41.0
56-57	37.10644511041604	39.0	35.5	41.0	31.0	41.0
58-59	36.956201924318975	39.0	35.0	40.0	31.0	41.0
60-61	36.72425106133886	38.5	35.0	40.0	31.0	41.0
62-63	36.45898985916921	38.0	35.0	40.0	30.5	41.0
64-65	36.23179872255996	38.0	34.5	40.0	30.0	41.0
66-67	35.93615408508335	37.0	34.0	40.0	30.0	41.0
68-69	35.61027538485021	37.0	34.0	39.0	29.0	41.0
70-71	35.305052447721835	36.0	34.0	39.0	29.0	40.5
72-73	34.993668098130485	36.0	34.0	39.0	29.0	40.0
74-75	34.60505245753059	35.0	33.5	38.0	29.0	40.0
76-77	33.548645868137854	34.5	32.0	37.0	27.5	39.0
78-79	33.8864399937417	35.0	33.0	37.0	28.5	39.0
80-81	33.7609011740968	35.0	33.0	36.0	29.0	39.0
82-83	33.53409033669753	35.0	33.0	36.0	29.0	37.5
84-85	33.311604915318526	35.0	33.0	35.5	29.0	37.0
86-87	33.100081169856	35.0	32.0	35.0	28.5	37.0
88-89	32.87171455943614	34.0	32.0	35.0	28.0	36.0
90-91	32.727981486894784	34.0	32.0	35.0	27.0	36.0
92-93	32.63618096457175	34.0	32.0	35.0	27.0	36.0
94-95	32.582910207682644	34.0	32.0	35.0	27.0	35.0
96-97	32.51879237620075	34.0	32.0	35.0	27.0	35.0
98-99	32.64631407998249	34.0	32.0	35.0	29.0	35.0
100-101	31.721854035481662	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	16.0
20	93.0
21	516.0
22	2069.0
23	6951.0
24	17846.0
25	37703.0
26	67112.0
27	106719.0
28	152180.0
29	201843.0
30	257355.0
31	321276.0
32	399557.0
33	505241.0
34	668095.0
35	915831.0
36	1371482.0
37	2129222.0
38	2340972.0
39	1243260.0
40	48987.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.36262637192422	21.34766518119516	13.97016099566365	25.319547451216973
2	14.690192012341441	22.63828303379907	39.29237196042838	23.379152993431106
3	17.334464915277728	26.83001665319045	31.27324832078477	24.56227011074705
4	12.375853390689562	18.631315767385992	38.44281988243048	30.550010959493967
5	12.762767631296734	38.206046731209206	36.208071498290586	12.82311413920348
6	30.204325827416028	38.49291961481993	18.409362769039443	12.893391788724598
7	26.134067817839146	32.62089126808079	23.78167496855756	17.4633659455225
8	24.663851237427657	36.70971458343678	21.467709708283834	17.15872447085173
9	25.72821578147338	15.861478361598797	20.99130209865774	37.41900375827009
10-11	24.096252216905025	26.720889896990347	29.441332522043055	19.74152536406157
12-13	24.757316990923382	24.288895056737207	29.305321276136876	21.64846667620254
14-15	21.93206005969061	25.71097524551783	26.476636618787204	25.88032807600436
16-17	21.85121948519267	28.6960425693609	27.29302187088789	22.159716074558546
18-19	21.879324030175848	28.09792791176996	28.587967680804216	21.43478037724998
20-21	22.560353601528533	27.4192338741637	28.339254347372012	21.681158176935753
22-23	22.251778549059026	27.430815749215746	28.20191065608019	22.115495045645037
24-25	22.12205100463743	27.541468080593788	28.288937772010573	22.0475431427582
26-27	22.13550042533662	27.66518243116385	28.218347190576303	21.980969952923232
28-29	22.02719038880717	27.634675175088642	28.283267769564326	22.05486666653987
30-31	22.325115419713097	27.525202199111508	28.206979924062914	21.94270245711248
32-33	22.044075622398854	27.748303501970916	28.156455828127353	22.051165047502877
34-35	22.168779404141603	27.74272582434587	28.217920300073747	21.87057447143878
36-37	22.26212774894339	27.623281982552566	28.091870543207737	22.02271972529631
38-39	22.047874801350236	27.712346815787892	28.215095740801573	22.0246826420603
40-41	22.244463845803082	27.57133803226338	28.147592018577893	22.036606103355638
42-43	22.189796112490466	27.625340443416025	28.00905390216583	22.175809541927684
44-45	22.207893689863976	27.741671327750577	27.871187989269547	22.179246993115903
46-47	22.362073958890903	27.636773453380364	27.624640164849417	22.376512422879312
48-49	22.19504758978534	27.753113406217985	27.66085143551622	22.39098756848045
50-51	22.175142853330158	27.858711651394774	27.639903619346423	22.326241875928645
52-53	22.33043641539607	27.78852978662993	27.58797034633108	22.293063451642926
54-55	22.28347538553663	27.721879453683734	27.550907274366914	22.44373788641272
56-57	22.364849393040885	27.868144924597498	27.430365803391382	22.33663987897023
58-59	22.258517065637797	27.900072961339124	27.56931381166134	22.272096161361738
60-61	22.402281363199933	27.936038162895926	27.40861528908684	22.253065184817306
62-63	22.331948677621014	28.029305230856146	27.38510863396897	22.253637457553875
64-65	22.324886606723172	27.936863038374955	27.387482491676675	22.350767863225197
66-67	22.34552161329022	27.928932729351096	27.30577181912167	22.41977383823701
68-69	22.32057169952774	27.88767296028693	27.36507515973747	22.426680180447857
70-71	22.299570364483337	27.684594981248623	27.47442055635061	22.541414097917432
72-73	22.355138850030187	27.722596250792243	27.427607365466166	22.494657533711408
74-75	22.20461889339244	27.863827904983303	27.37128206938128	22.560271132242978
76-77	22.361804804044823	27.77787017593202	27.32746227186844	22.532862748154724
78-79	22.315846718059575	27.796483741653955	27.28363400051201	22.604035539774465
80-81	22.350058842918102	27.786138108406313	27.322727234461254	22.541075814214338
82-83	22.308415327362695	27.779560790087103	27.4030736514947	22.508950231055493
84-85	22.350605410733554	27.780313827874203	27.344038469223293	22.525042292168955
86-87	22.25100529525484	27.814540111380726	27.420012727171223	22.514441866193216
88-89	22.283229219284856	27.79527610851773	27.351214660052026	22.57028001214539
90-91	22.31527587039831	27.806273695166883	27.415416326260257	22.463034108174554
92-93	22.28014873227958	27.779476438760227	27.43793223003494	22.502442598925256
94-95	22.11396956465895	27.847208559373566	27.527969346383347	22.51085252958414
96-97	22.033294203986646	27.889322788842406	27.584422679289457	22.492960327881494
98-99	22.188294178821078	28.483176904279144	27.643926261514185	21.684602655385596
100-101	25.108234915911183	36.260718131593855	28.74968043037774	9.881366522117224
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2566.0
1	2356.5
2	2965.5
3	3972.0
4	5105.5
5	7482.0
6	9381.5
7	10644.0
8	11427.0
9	11303.5
10	10759.0
11	10381.0
12	11485.5
13	14979.0
14	23020.0
15	33489.0
16	41500.5
17	45700.0
18	46745.5
19	46044.5
20	44736.5
21	44414.5
22	46400.5
23	52657.0
24	64012.0
25	80001.0
26	100411.0
27	123720.5
28	149057.0
29	175439.5
30	200497.5
31	225281.0
32	250906.0
33	275381.5
34	298961.0
35	322363.5
36	348162.0
37	371272.5
38	390453.0
39	407546.0
40	423213.0
41	439237.0
42	450983.0
43	461981.5
44	472470.5
45	478695.5
46	485046.0
47	489351.5
48	486470.0
49	478957.0
50	470262.0
51	457014.5
52	435940.5
53	412541.0
54	389079.5
55	363404.0
56	335256.0
57	304017.5
58	271479.0
59	237067.5
60	200385.0
61	164970.0
62	133052.0
63	104831.5
64	80071.5
65	60461.5
66	45495.0
67	34865.0
68	26736.0
69	19638.0
70	14214.5
71	10216.0
72	7161.5
73	4950.0
74	3386.0
75	2254.0
76	1522.5
77	1050.0
78	733.0
79	495.5
80	337.0
81	237.0
82	173.0
83	124.5
84	91.5
85	66.5
86	52.0
87	43.0
88	31.0
89	22.0
90	17.0
91	12.5
92	9.5
93	6.5
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001167279704674529
3	0.0
4	3.1498023776931733E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0034045658053007094
18-19	0.0
20-21	0.0021586363310631056
22-23	0.0
24-25	2.965662677093504E-4
26-27	0.003221792109112596
28-29	0.0
30-31	1.533366757630939E-4
32-33	7.644029123005199E-4
34-35	6.132591305389939E-4
36-37	0.009788207599164746
38-39	0.011311736668859089
40-41	0.023980605685152135
42-43	0.0020042810083503782
44-45	0.0013751011129037082
46-47	0.0018470935162038893
48-49	0.0018117962572023949
50-51	6.13537427240213E-5
52-53	1.0868735435571041E-4
54-55	0.006300647284051474
56-57	0.00375864621537819
58-59	0.0038464532545067565
60-61	0.001708364915725866
62-63	5.555926567985262E-4
64-65	8.62513197720327E-4
66-67	6.127517650973158E-4
68-69	1.1640444141146206E-5
70-71	5.915074087190204E-6
72-73	1.5626933645214875E-4
74-75	3.053061662136839E-4
76-77	0.0014087072380556986
78-79	0.002303740700540407
80-81	0.01190661767625746
82-83	0.010422174619119567
84-85	0.013465078897238006
86-87	0.007325625192933566
88-89	0.08133006306452785
90-91	4.7362716691497924E-4
92-93	5.7881457617170886E-5
94-95	2.3757832846124524E-4
96-97	0.014692470013114162
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	294.0
20-21	1068.0
22-23	1908.0
24-25	3826.0
26-27	6406.0
28-29	13190.0
30-31	30827.0
32-33	42541.0
34-35	61140.0
36-37	95621.0
38-39	104106.0
40-41	102685.0
42-43	116672.0
44-45	136237.0
46-47	137562.0
48-49	130178.0
50-51	120012.0
52-53	119213.0
54-55	131435.0
56-57	136976.0
58-59	136885.0
60-61	134300.0
62-63	128951.0
64-65	134639.0
66-67	142658.0
68-69	137866.0
70-71	135605.0
72-73	130625.0
74-75	131133.0
76-77	133272.0
78-79	139621.0
80-81	140828.0
82-83	134284.0
84-85	135371.0
86-87	142732.0
88-89	150893.0
90-91	160946.0
92-93	170306.0
94-95	211422.0
96-97	273916.0
98-99	857785.0
100-101	5438393.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.21367562299143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.67249212611611	80.86767483964442
2	6.080904153130765	10.728378129175574
3	1.3162525728014873	3.483344324851149
4	0.44542069706449683	1.57168787546457
5	0.19865396323479495	0.8761998137007933
6	0.09833299265346587	0.5204588830182503
7	0.05343759963695112	0.3299748956311704
8	0.0333090467755278	0.23506507580539657
9	0.024112566739160526	0.1914352326689435
>10	0.07499372135973317	1.0440523490295883
>50	0.0017614460475671259	0.10426425564025546
>100	3.2911443989966133E-4	0.04746432536990155
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.852824928054808E-5	0.0	0.0	0.0
3	0.0	1.852824928054808E-5	0.0	9.26412464027404E-6	0.0
4	0.0	5.5584747841644244E-5	0.0	5.5584747841644244E-5	0.0
5	0.0	8.337712176246636E-5	0.0	5.5584747841644244E-5	0.0
6	4.63206232013702E-5	8.337712176246636E-5	0.0	5.5584747841644244E-5	0.0
7	4.63206232013702E-5	8.337712176246636E-5	0.0	7.411299712219232E-5	0.0
8	4.63206232013702E-5	9.26412464027404E-5	0.0	8.337712176246636E-5	0.0
9	4.63206232013702E-5	1.7601836816520677E-4	0.0	1.4822599424438464E-4	0.0
10-11	4.63206232013702E-5	1.806504304853438E-4	0.0	1.7601836816520677E-4	0.0
12-13	4.63206232013702E-5	1.852824928054808E-4	0.0	2.3623517832698803E-4	9.26412464027404E-6
14-15	4.63206232013702E-5	2.3623517832698803E-4	0.0	2.825558015283582E-4	1.852824928054808E-5
16-17	1.2969774496383657E-4	2.4086724064712505E-4	0.0	3.010840508089063E-4	1.852824928054808E-5
18-19	1.2969774496383657E-4	2.686596145679472E-4	0.0	3.5203673633041354E-4	2.7792373920822122E-5
20-21	1.2969774496383657E-4	3.1034817544918036E-4	0.0	3.8446117257137267E-4	2.7792373920822122E-5
22-23	1.343298072839736E-4	3.5203673633041354E-4	0.0	4.771024189741131E-4	3.242443624095914E-5
24-25	1.4359393192424762E-4	3.751970479310986E-4	0.0	5.234230421754832E-4	4.63206232013702E-5
26-27	1.574901188846587E-4	3.890932348915097E-4	0.0	6.299604755386347E-4	4.63206232013702E-5
28-29	1.574901188846587E-4	3.890932348915097E-4	0.0	0.001116327019153022	4.63206232013702E-5
30-31	1.574901188846587E-4	4.4004592041301687E-4	0.0	0.0028626145138446785	4.63206232013702E-5
32-33	1.621221812047957E-4	4.63206232013702E-4	0.0	0.005850294710333057	4.63206232013702E-5
34-35	1.6675424352493272E-4	5.002627305747982E-4	0.0	0.010046943172377195	5.5584747841644244E-5
36-37	1.6675424352493272E-4	5.373192291358944E-4	0.0	0.016619839604651628	5.5584747841644244E-5
38-39	1.6675424352493272E-4	5.512154160963054E-4	0.0	0.025355909140430045	5.5584747841644244E-5
40-41	1.6675424352493272E-4	6.206963508983607E-4	0.0	0.034272629106693805	5.5584747841644244E-5
42-43	1.6675424352493272E-4	6.345925378587717E-4	0.0	0.04223514423500935	5.5584747841644244E-5
44-45	1.7601836816520677E-4	6.948093480205529E-4	0.0	0.051999531605858185	5.5584747841644244E-5
46-47	1.852824928054808E-4	7.318658465816491E-4	0.0	0.06256526575809074	5.5584747841644244E-5
48-49	1.852824928054808E-4	7.503940958621972E-4	0.0	0.07256125624494641	5.5584747841644244E-5
50-51	2.084428044061659E-4	7.920826567434304E-4	0.0	0.08260356735500349	5.5584747841644244E-5
52-53	2.1307486672630292E-4	7.967147190635675E-4	0.0	0.09180747518511574	6.484887248191828E-5
54-55	2.1307486672630292E-4	8.245070929843897E-4	0.0	0.10198411610245678	7.411299712219232E-5
56-57	2.1307486672630292E-4	8.430353422649376E-4	0.0	0.11210517227195615	7.411299712219232E-5
58-59	2.1307486672630292E-4	8.476674045850747E-4	0.0	0.12205484213561048	7.411299712219232E-5
60-61	2.3160311600685103E-4	8.893559654663079E-4	0.0	0.13159689051509274	7.411299712219232E-5
62-63	2.4086724064712505E-4	9.26412464027404E-4	0.0	0.14063404410168007	7.411299712219232E-5
64-65	2.4086724064712505E-4	0.0010190537104301444	0.0	0.15094501482630507	7.411299712219232E-5
66-67	2.501313652873991E-4	0.0010283178350704184	0.0	0.1608205716928372	7.411299712219232E-5
68-69	2.501313652873991E-4	0.0010422140220308295	0.0	0.17031166738679795	7.411299712219232E-5
70-71	2.5939548992767313E-4	0.0010653743336315146	0.0	0.17985834782860036	7.411299712219232E-5
72-73	2.964519884887693E-4	0.0010839025829120627	0.0	0.1876726369626715	7.411299712219232E-5
74-75	2.964519884887693E-4	0.001107062894512748	0.0	0.19461609838055688	7.411299712219232E-5
76-77	2.964519884887693E-4	0.001148751455393981	0.0	0.20100371232002584	7.411299712219232E-5
78-79	2.964519884887693E-4	0.001167279704674529	0.0	0.20696517652604218	7.411299712219232E-5
80-81	2.964519884887693E-4	0.0011765438293148032	0.0	0.2101798277762173	7.411299712219232E-5
82-83	2.964519884887693E-4	0.0011765438293148032	0.0	0.21221793519707757	8.337712176246636E-5
84-85	2.964519884887693E-4	0.0012182323901960362	0.0	0.21396422269176923	8.337712176246636E-5
86-87	2.964519884887693E-4	0.0012321285771564473	0.0	0.21420045787009623	8.337712176246636E-5
88-89	2.964519884887693E-4	0.0012413927017967214	0.0	0.2143347876773802	8.337712176246636E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9590	0.0	44.543816	1
GTATCAA	16690	0.0	31.720936	1
TCAACGC	19615	0.0	25.980513	4
ATCAACG	20135	0.0	25.309551	3
CAACGCA	20330	0.0	25.046793	5
AACGCAG	21050	0.0	24.228483	6
TATCAAC	21675	0.0	23.828833	2
GTACATG	27260	0.0	21.42264	1
TACATGG	27705	0.0	20.801394	2
ACGCAGA	25030	0.0	20.392076	7
ACATGGG	27620	0.0	20.221125	3
CGCAGAG	25650	0.0	19.930681	8
CTTATAC	8020	0.0	18.191305	1
CATGGGG	19820	0.0	18.106323	4
AGAGTAC	27215	0.0	16.283411	10-11
GAGTACT	16850	0.0	16.094137	12-13
TATACAC	11565	0.0	16.039396	3
GCAGAGT	31385	0.0	15.953947	9
CAGAGTA	30250	0.0	15.123981	10-11
TATGCCG	4880	0.0	14.89541	42-43
>>END_MODULE