FastQCFastQC Report
Fri 27 May 2016
SRR2139715_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139715_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6960896
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65550.040.276121
GTATCAA105700.032.4050521
TCAACGC124650.026.4994144
ATCAACG128300.025.7772393
CAACGCA129650.025.4145185
AACGCAG135050.024.4284386
TATCAAC140200.023.9376532
GTACATG174600.020.8523221
TACATGG172000.020.623562
ACGCAGA162050.020.3582867
CGCAGAG165550.019.9033058
ACATGGG175150.019.7647763
CATGGGG130150.017.3468784
AGAGTAC176850.016.2286810-11
GCAGAGT206300.015.7943849
GAGTACT112250.015.56567912-13
CAGAGTA196050.015.03359210-11
TATACCG4154.129106E-1014.7032245
AGTACTT126950.013.71520512-13
GAGTACA126550.013.6937711
Produced by FastQC (version 0.11.5)