##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139715_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6960896
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12762121428046	33.0	31.0	34.0	30.0	34.0
2	32.25024651998823	33.0	31.0	34.0	30.0	34.0
3	32.30420422888088	34.0	31.0	34.0	30.0	34.0
4	35.942420630907286	37.0	35.0	37.0	35.0	37.0
5	35.807619737459085	37.0	35.0	37.0	35.0	37.0
6	35.82521258757493	37.0	35.0	37.0	35.0	37.0
7	35.73766250781509	37.0	35.0	37.0	33.0	37.0
8	35.69947532616491	37.0	35.0	37.0	33.0	37.0
9	37.326813674561436	39.0	37.0	39.0	34.0	39.0
10-11	37.22083328065813	39.0	37.0	39.0	33.0	39.0
12-13	37.17981729650896	39.0	37.0	39.0	33.0	39.0
14-15	38.370330486190284	40.0	38.0	41.0	33.0	41.0
16-17	38.38671997110717	40.0	38.0	41.0	33.0	41.0
18-19	38.37537401794252	40.0	38.0	41.0	33.5	41.0
20-21	38.343254749288846	40.0	38.0	41.0	34.0	41.0
22-23	38.24087057591054	40.0	38.0	41.0	33.0	41.0
24-25	38.153566403238756	40.0	37.5	41.0	33.0	41.0
26-27	37.98150636589371	40.0	37.0	41.0	33.0	41.0
28-29	37.84527374072397	40.0	37.0	41.0	33.0	41.0
30-31	37.696902730464004	40.0	37.0	41.0	32.0	41.0
32-33	37.89201240203246	40.0	37.0	41.0	33.0	41.0
34-35	37.87265104152851	40.0	37.0	41.0	33.0	41.0
36-37	37.787962559217206	40.0	37.0	41.0	33.0	41.0
38-39	37.67862448592251	40.0	37.0	41.0	32.5	41.0
40-41	37.62733103059277	40.0	37.0	41.0	32.0	41.0
42-43	37.51723559084255	40.0	36.5	41.0	32.0	41.0
44-45	37.55913991879911	39.5	36.5	41.0	32.5	41.0
46-47	37.69869224939191	40.0	37.0	41.0	32.5	41.0
48-49	37.625209083713784	40.0	37.0	41.0	32.0	41.0
50-51	37.502064665837054	40.0	36.0	41.0	32.0	41.0
52-53	37.34781831324241	39.0	36.0	41.0	31.0	41.0
54-55	37.23784912112053	39.0	36.0	41.0	31.0	41.0
56-57	37.06462700294385	39.0	35.0	40.5	31.0	41.0
58-59	36.909790520344146	39.0	35.0	40.0	31.0	41.0
60-61	36.6752259941272	38.0	35.0	40.0	31.0	41.0
62-63	36.40553410429316	38.0	35.0	40.0	30.0	41.0
64-65	36.177632246907365	38.0	34.5	40.0	30.0	41.0
66-67	35.87762714292219	37.0	34.0	40.0	29.5	41.0
68-69	35.549927451607985	36.5	34.0	39.0	29.0	41.0
70-71	35.2465885117735	36.0	34.0	39.0	29.0	40.5
72-73	34.93466630451914	36.0	34.0	39.0	29.0	40.0
74-75	34.54704405730045	35.0	33.0	38.0	29.0	40.0
76-77	33.49424214352803	34.5	31.5	36.5	27.5	39.0
78-79	33.83962052853042	35.0	33.0	37.0	28.5	39.0
80-81	33.718335178926395	35.0	33.0	36.0	29.0	39.0
82-83	33.492746245635615	35.0	33.0	36.0	29.0	37.5
84-85	33.27062037610141	35.0	32.5	35.5	28.5	37.0
86-87	33.05923102285301	34.5	32.0	35.0	28.0	37.0
88-89	32.83025595023751	34.0	32.0	35.0	27.0	36.0
90-91	32.68597722833813	34.0	32.0	35.0	27.0	36.0
92-93	32.5904330495586	34.0	32.0	35.0	27.0	36.0
94-95	32.53270699938356	34.0	32.0	35.0	27.0	35.0
96-97	32.47052332821109	34.0	32.0	35.0	27.0	35.0
98-99	32.604644773633765	34.0	32.0	35.0	28.5	35.0
100-101	31.684934992884283	33.0	30.5	34.5	26.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	51.0
21	317.0
22	1377.0
23	4556.0
24	11616.0
25	24744.0
26	44582.0
27	69624.0
28	99789.0
29	132862.0
30	169898.0
31	210639.0
32	262168.0
33	330777.0
34	436490.0
35	598992.0
36	895255.0
37	1381775.0
38	1485327.0
39	770613.0
40	29437.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.32044093174213	21.47413206575705	13.982697055091759	25.222729947409068
2	14.716993709215457	22.611081429090866	39.3636120587569	23.308312802936783
3	17.50322084972969	26.78512938564231	31.079691465006803	24.6319582996212
4	12.40520044115169	18.54553833852114	38.40790901023737	30.6413522100898
5	12.800119409915045	38.12927243849067	36.20990746018904	12.860700691405244
6	30.323912323930713	38.48191382258835	18.304913620315546	12.889260233165386
7	26.224540633849436	32.473218964914864	23.77583288128425	17.526407519951455
8	24.59141754164981	36.93587722040381	21.364620301754258	17.10808493619212
9	25.610955830973488	15.924975750248244	20.997828440476628	37.466239978301644
10-11	24.180536528630796	26.725122742819313	29.40111445423118	19.69322627431871
12-13	24.792907407322275	24.266531205178186	29.31534963315067	21.625211754348868
14-15	21.90441431677761	25.745083678882718	26.424608268820567	25.925893735519107
16-17	21.841392968911567	28.760293498726085	27.271185487376275	22.127128044986073
18-19	21.8882813361958	28.098530706391823	28.624332269868706	21.388855687543675
20-21	22.52004628474948	27.454488195492438	28.365909563714226	21.659555956043857
22-23	22.275301125490667	27.446884158221156	28.20466123530473	22.073153480983443
24-25	22.10483910896336	27.539637729914784	28.32081210861966	22.034711052502193
26-27	22.150931741587453	27.636656223638113	28.231382602055334	21.9810294327191
28-29	22.115487201401713	27.628965288516564	28.293893125652197	21.961654384429526
30-31	22.27229459154284	27.61548570642571	28.14868870941326	21.963530992618193
32-33	22.151957831151154	27.71326529904291	28.13555507632321	21.99922179348272
34-35	22.23592836091523	27.64561881305065	28.15663191764976	21.96182090838436
36-37	22.18557424309476	27.662385059994456	28.094677969286213	22.057362727624565
38-39	22.03786660482767	27.69079048382207	28.236889146747927	22.03445376460233
40-41	22.25242112374793	27.54090452374997	28.13239780576369	22.07427654673841
42-43	22.14458605718428	27.687157028841003	27.975295819962508	22.192961094012205
44-45	22.218525274581964	27.730813538408032	27.832683107967306	22.217978079042698
46-47	22.304580082719788	27.675467676368648	27.651549629177865	22.368402611733703
48-49	22.17663320238615	27.826389855942494	27.633093556048866	22.36388338562249
50-51	22.187695236268617	27.870455500013787	27.632196652258596	22.309652611459
52-53	22.341601796530416	27.760939145720986	27.55353159860214	22.34392745914646
54-55	22.269047192613147	27.71318008179822	27.581037399736186	22.436735325852446
56-57	22.320215027460467	27.854706283362358	27.452538105104935	22.372540584072244
58-59	22.301586457249034	27.887831597956982	27.436752322303587	22.373829622490398
60-61	22.385449734959206	27.838621703593585	27.44831295766039	22.327615603786814
62-63	22.325211151747602	27.953239115263777	27.408665928962183	22.312883804026438
64-65	22.316511006902104	27.916833603217096	27.378820949260614	22.38783444062019
66-67	22.386644969084813	27.930783647806813	27.26352238000249	22.419049003105883
68-69	22.339888202101676	27.867817913841687	27.36462833413173	22.427665549924907
70-71	22.358253261180803	27.652067619437688	27.421954570002573	22.567724549378934
72-73	22.33829530102782	27.762580068616966	27.36279597116858	22.536328659186633
74-75	22.222219108080555	27.89227698160075	27.381293045807126	22.504210864511577
76-77	22.415655422367617	27.801844254556695	27.326491276576892	22.4560090464988
78-79	22.333286980900162	27.82555850021013	27.289040336838827	22.552114182050882
80-81	22.318222137783554	27.81358624894518	27.32045419061172	22.54773742265954
82-83	22.316863528862598	27.799020694174807	27.38395074168999	22.500165035272605
84-85	22.3608170610784	27.782269318998242	27.382775600421745	22.47413801950161
86-87	22.26307529195587	27.77853961461949	27.459871192349805	22.498513901074833
88-89	22.230216814033252	27.78783431782863	27.419679848248112	22.562269019890007
90-91	22.300583333010433	27.823324881914786	27.432236887708072	22.443854897366712
92-93	22.307348256320203	27.78856855690286	27.450464818316313	22.453618368460624
94-95	22.10980656236125	27.83408600684629	27.549397976021144	22.50670945477131
96-97	22.06184126875855	27.83233190786473	27.642192858391784	22.463633964984933
98-99	22.188011609902823	28.49423358793971	27.698448374841757	21.619306427315706
100-101	25.09577377852425	36.25362910524042	28.813606347696556	9.836990768538772
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1656.0
1	1541.0
2	1997.5
3	2689.0
4	3411.5
5	4895.5
6	6120.5
7	7063.0
8	7649.5
9	7580.0
10	7165.5
11	6765.0
12	7355.5
13	9605.0
14	14794.5
15	21663.0
16	26905.0
17	29519.5
18	30470.0
19	30466.0
20	29826.5
21	29694.5
22	31042.0
23	35208.5
24	42583.5
25	53129.5
26	66646.5
27	81000.0
28	96700.5
29	113918.5
30	129643.0
31	145210.0
32	161200.0
33	176434.0
34	191264.5
35	205531.5
36	221179.0
37	235404.5
38	247455.0
39	257748.5
40	267910.0
41	277727.0
42	283484.0
43	289406.0
44	295795.0
45	300496.0
46	303729.0
47	305678.0
48	305310.5
49	302177.5
50	297180.5
51	289437.5
52	278661.5
53	266800.0
54	253917.0
55	237694.0
56	219946.5
57	201124.0
58	179493.5
59	156439.5
60	132424.0
61	109052.0
62	88188.5
63	69294.0
64	52308.0
65	38747.5
66	28830.5
67	21996.0
68	16885.0
69	12329.0
70	8926.5
71	6371.0
72	4449.0
73	3112.0
74	2099.0
75	1427.5
76	1005.5
77	681.5
78	458.0
79	323.5
80	230.5
81	156.0
82	123.5
83	97.0
84	67.0
85	46.5
86	33.0
87	22.5
88	13.5
89	8.5
90	5.5
91	4.0
92	3.5
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00113491136773197
3	0.0
4	3.59149167003788E-4
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0036274065867382592
18-19	0.0
20-21	0.002305851439235607
22-23	0.0
24-25	4.311574183759723E-4
26-27	0.0034866393060135614
28-29	0.0
30-31	1.7292806776013407E-4
32-33	9.756394395088732E-4
34-35	7.112271782257653E-4
36-37	0.009831675291173902
38-39	0.010944952563764852
40-41	0.025074014846048896
42-43	0.0018258650693227618
44-45	0.0011703767609990336
46-47	0.001778805737549457
48-49	0.0017630945461010608
50-51	4.739580959428634E-5
52-53	1.1987937258013495E-4
54-55	0.006527834906444478
56-57	0.0037379855733430476
58-59	0.0041223116460955556
60-61	0.0017193179701597196
62-63	5.296545464905559E-4
64-65	7.966132852063458E-4
66-67	6.941163012071121E-4
68-69	0.0
70-71	9.0613517851452E-6
72-73	1.4722279833365875E-4
74-75	2.802719647036699E-4
76-77	0.0012809004179625504
78-79	0.00201542232017539
80-81	0.011382222793077764
82-83	0.009736382695162546
84-85	0.013257800107322146
86-87	0.006731978230581733
88-89	0.08181193459135302
90-91	3.3366416314498494E-4
92-93	3.3032986409899066E-5
94-95	3.388459426756247E-4
96-97	0.01394399954314132
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	218.0
20-21	650.0
22-23	1331.0
24-25	2702.0
26-27	4208.0
28-29	8288.0
30-31	18998.0
32-33	26622.0
34-35	37950.0
36-37	59607.0
38-39	64613.0
40-41	64118.0
42-43	72047.0
44-45	84738.0
46-47	85073.0
48-49	80936.0
50-51	74572.0
52-53	73348.0
54-55	80573.0
56-57	83403.0
58-59	83604.0
60-61	81284.0
62-63	77722.0
64-65	81536.0
66-67	87360.0
68-69	86029.0
70-71	84927.0
72-73	82148.0
74-75	82195.0
76-77	83263.0
78-79	88780.0
80-81	89325.0
82-83	85923.0
84-85	85524.0
86-87	90445.0
88-89	96134.0
90-91	103334.0
92-93	109942.0
94-95	137054.0
96-97	179148.0
98-99	564410.0
100-101	3576814.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.23348217377216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.85144528000187	81.04372859748253
2	5.908050905802951	10.425758085578064
3	1.2872114667639352	3.4072545001977232
4	0.45301432702643313	1.598841261926007
5	0.19302601494468166	0.8515678724347924
6	0.09960487408651965	0.5273090929280251
7	0.06175504365696769	0.3814203780553306
8	0.03822659360225798	0.269829237213509
9	0.023437485677999262	0.18611738772910094
>10	0.08229837248510634	1.16444898041708
>50	0.001566742828007895	0.09166248188100526
>100	3.387043506075333E-4	0.040622492498847716
>500	2.4188772618899593E-5	0.011439631657938565
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.436596668015152E-5
2	0.0	5.746386672060608E-5	0.0	0.0	1.436596668015152E-5
3	0.0	5.746386672060608E-5	0.0	0.0	1.436596668015152E-5
4	0.0	1.1492773344121216E-4	0.0	0.0	1.436596668015152E-5
5	0.0	1.1492773344121216E-4	0.0	1.436596668015152E-5	1.436596668015152E-5
6	2.873193336030304E-5	1.1492773344121216E-4	0.0	1.436596668015152E-5	1.436596668015152E-5
7	2.873193336030304E-5	1.1492773344121216E-4	0.0	2.873193336030304E-5	1.436596668015152E-5
8	2.873193336030304E-5	1.1492773344121216E-4	0.0	2.873193336030304E-5	1.436596668015152E-5
9	2.873193336030304E-5	1.7239160016181826E-4	0.0	4.3097900040454564E-5	4.3097900040454564E-5
10-11	2.873193336030304E-5	1.7239160016181826E-4	0.0	4.3097900040454564E-5	4.3097900040454564E-5
12-13	2.873193336030304E-5	1.7957458350189402E-4	0.0	4.3097900040454564E-5	5.028088338053032E-5
14-15	2.873193336030304E-5	2.729533669228789E-4	0.0	4.3097900040454564E-5	5.746386672060608E-5
16-17	5.746386672060608E-5	2.729533669228789E-4	0.0	4.3097900040454564E-5	7.18298334007576E-5
18-19	5.746386672060608E-5	2.729533669228789E-4	0.0	5.746386672060608E-5	8.619580008090913E-5
20-21	5.746386672060608E-5	2.729533669228789E-4	0.0	1.436596668015152E-4	8.619580008090913E-5
22-23	5.746386672060608E-5	3.0168530028318194E-4	0.0	2.2985546688242432E-4	9.337878342098489E-5
24-25	5.746386672060608E-5	3.2323425030340923E-4	0.0	3.519661836637123E-4	1.0056176676106065E-4
26-27	5.746386672060608E-5	3.30417233643485E-4	0.0	7.613962340480306E-4	1.0056176676106065E-4
28-29	5.746386672060608E-5	3.3760021698356075E-4	0.0	0.003455014986576441	1.0056176676106065E-4
30-31	5.746386672060608E-5	3.5914916700378804E-4	0.0	0.012304450461549777	1.0056176676106065E-4
32-33	5.746386672060608E-5	3.5914916700378804E-4	0.0	0.028624188610201905	1.2929370012136369E-4
34-35	5.746386672060608E-5	4.381619837446214E-4	0.0	0.0528954893163179	1.2929370012136369E-4
36-37	5.746386672060608E-5	5.171748004854547E-4	0.0	0.08227389117722776	1.2929370012136369E-4
38-39	5.746386672060608E-5	5.459067338457578E-4	0.0	0.11856950599463056	1.2929370012136369E-4
40-41	7.18298334007576E-5	6.177365672465154E-4	0.0	0.15085701610827112	1.2929370012136369E-4
42-43	7.18298334007576E-5	6.464685006068185E-4	0.0	0.17648590066566144	1.2929370012136369E-4
44-45	7.901281674083337E-5	7.398472840278033E-4	0.0	0.208205955095436	1.2929370012136369E-4
46-47	8.619580008090913E-5	8.044941340884852E-4	0.0	0.23785012733992866	1.5084265014159097E-4
48-49	8.619580008090913E-5	8.044941340884852E-4	0.0	0.2565402499908058	1.5802563348166673E-4
50-51	1.1492773344121216E-4	8.404090507888639E-4	0.0	0.271919017321908	1.5802563348166673E-4
52-53	1.1492773344121216E-4	8.475920341289397E-4	0.0	0.28333277784928834	1.652086168217425E-4
54-55	1.2211071678128792E-4	8.475920341289397E-4	0.0	0.2955438495274172	1.7239160016181826E-4
56-57	1.2929370012136369E-4	8.763239674892427E-4	0.0	0.3055856602368431	1.7239160016181826E-4
58-59	1.2929370012136369E-4	9.122388841896216E-4	0.0	0.3145428404619175	1.7239160016181826E-4
60-61	1.436596668015152E-4	9.194218675296973E-4	0.0	0.3232917141701298	1.7239160016181826E-4
62-63	1.5802563348166673E-4	9.26604850869773E-4	0.0	0.332033404895002	1.8675756684196978E-4
64-65	1.5802563348166673E-4	0.0010918134676915156	0.0	0.3408900233533154	1.8675756684196978E-4
66-67	1.652086168217425E-4	0.0011205454010518186	0.0	0.350048327111912	1.9394055018204554E-4
68-69	1.7239160016181826E-4	0.0011564603177521974	0.0	0.35901269032032657	2.0830651686219704E-4
70-71	1.7239160016181826E-4	0.0011995582177926518	0.0	0.3679698705454011	2.2267248354234856E-4
72-73	1.8675756684196978E-4	0.0012211071678128792	0.0	0.37512412195211653	2.5858740024272737E-4
74-75	1.8675756684196978E-4	0.0012498391011731822	0.0	0.38168936872494574	2.5858740024272737E-4
76-77	1.8675756684196978E-4	0.0012857540178735612	0.0	0.38737829153028575	2.5858740024272737E-4
78-79	1.8675756684196978E-4	0.001314485951233864	0.0	0.39278707798536283	2.5858740024272737E-4
80-81	1.8675756684196978E-4	0.00132166893457394	0.0	0.3956099904380126	2.729533669228789E-4
82-83	1.8675756684196978E-4	0.0013360349012540916	0.0	0.39752066400647273	2.873193336030304E-4
84-85	1.8675756684196978E-4	0.0013647668346143944	0.0	0.3990219075245486	2.873193336030304E-4
86-87	1.8675756684196978E-4	0.0013647668346143944	0.0	0.39924458000809093	2.873193336030304E-4
88-89	2.154895002022728E-4	0.001407864734654849	0.0	0.3993020438748115	2.945023169431062E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6555	0.0	40.27612	1
GTATCAA	10570	0.0	32.405052	1
TCAACGC	12465	0.0	26.499414	4
ATCAACG	12830	0.0	25.777239	3
CAACGCA	12965	0.0	25.414518	5
AACGCAG	13505	0.0	24.428438	6
TATCAAC	14020	0.0	23.937653	2
GTACATG	17460	0.0	20.852322	1
TACATGG	17200	0.0	20.62356	2
ACGCAGA	16205	0.0	20.358286	7
CGCAGAG	16555	0.0	19.903305	8
ACATGGG	17515	0.0	19.764776	3
CATGGGG	13015	0.0	17.346878	4
AGAGTAC	17685	0.0	16.22868	10-11
GCAGAGT	20630	0.0	15.794384	9
GAGTACT	11225	0.0	15.565679	12-13
CAGAGTA	19605	0.0	15.033592	10-11
TATACCG	415	4.129106E-10	14.703224	5
AGTACTT	12695	0.0	13.715205	12-13
GAGTACA	12655	0.0	13.693771	1
>>END_MODULE