FastQCFastQC Report
Fri 27 May 2016
SRR2139714_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139714_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11013013
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162250.053.967671
GTATCAA308650.039.3140451
TCAACGC366050.032.315234
ATCAACG369550.031.9757843
TATCAAC378000.031.5448652
CAACGCA376450.031.4566785
AACGCAG388900.030.4527656
ACGCAGA436850.027.1101747
CGCAGAG445950.026.614688
GTGGTAT73300.024.5942481
CTTATAC165250.024.1043111
TGGTATC68450.023.828592
GCAGAGT513850.023.0978249
GTACATG373500.021.6395361
GAGTACT306500.021.4820712-13
TACATGG371200.021.3689042
TATACAC213250.020.9934333
ACATGGG368000.020.8548893
CAGAGTA508050.020.19542510-11
TTATACA217800.019.352712
Produced by FastQC (version 0.11.5)