##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139714_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11013013
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.241185495740357	32.0	28.0	33.0	18.0	33.0
2	30.805278264903528	33.0	31.0	33.0	25.0	34.0
3	31.65534554440279	33.0	32.0	33.0	27.0	34.0
4	30.98623746289957	33.0	31.0	33.0	27.0	34.0
5	31.854599372578605	33.0	32.0	33.0	30.0	34.0
6	35.28989596216766	37.0	35.0	38.0	29.0	38.0
7	35.87323223898855	38.0	36.0	38.0	31.0	38.0
8	36.0295780092151	38.0	37.0	38.0	31.0	38.0
9	36.31897674142399	38.0	37.0	38.0	33.0	38.0
10-11	36.45097486037653	38.0	37.5	38.0	33.5	38.0
12-13	36.48757796799114	38.0	38.0	38.0	34.0	38.0
14-15	36.49817279794367	38.0	38.0	38.0	34.0	38.0
16-17	36.48895256911074	38.0	38.0	38.0	34.0	38.0
18-19	36.504627798042186	38.0	38.0	38.0	34.0	38.0
20-21	36.50227327892804	38.0	38.0	38.0	34.0	38.0
22-23	36.49105593794395	38.0	38.0	38.0	34.0	38.0
24-25	36.477721603527385	38.0	38.0	38.0	34.0	38.0
26-27	36.41689652194475	38.0	38.0	38.0	34.0	38.0
28-29	36.39318542714307	38.0	38.0	38.0	33.5	38.0
30-31	36.397436808329964	38.0	38.0	38.0	34.0	38.0
32-33	36.37195686657975	38.0	38.0	38.0	33.0	38.0
34-35	36.32298856787945	38.0	37.5	38.0	33.0	38.0
36-37	36.29897059012357	38.0	37.0	38.0	33.0	38.0
38-39	36.26205201886249	38.0	37.0	38.0	33.0	38.0
40-41	36.29196019377047	38.0	37.0	38.0	33.0	38.0
42-43	36.245972442237246	38.0	37.0	38.0	33.0	38.0
44-45	36.19080395554984	38.0	37.0	38.0	33.0	38.0
46-47	36.14541206049392	38.0	37.0	38.0	33.0	38.0
48-49	36.09384359973646	38.0	37.0	38.0	33.0	38.0
50-51	36.062484446662765	38.0	37.0	38.0	32.5	38.0
52-53	36.03670395355773	38.0	37.0	38.0	32.0	38.0
54-55	35.98860391532786	38.0	37.0	38.0	31.0	38.0
56-57	35.98484401901469	38.0	37.0	38.0	31.0	38.0
58-59	35.95315578941126	38.0	37.0	38.0	31.0	38.0
60-61	35.93437001018199	38.0	37.0	38.0	31.0	38.0
62-63	35.9288532897791	38.0	37.0	38.0	31.0	38.0
64-65	35.91714538051953	38.0	37.0	38.0	31.0	38.0
66-67	35.91780980070315	38.0	37.0	38.0	31.0	38.0
68-69	35.89810275763905	38.0	37.0	38.0	31.0	38.0
70-71	35.867320988620904	38.0	37.0	38.0	31.0	38.0
72-73	35.83827341394223	38.0	37.0	38.0	31.0	38.0
74-75	35.85440665600447	38.0	37.0	38.0	31.0	38.0
76-77	35.834423602758676	38.0	37.0	38.0	31.0	38.0
78-79	35.81717259394638	38.0	37.0	38.0	31.0	38.0
80-81	35.77308538600167	38.0	37.0	38.0	31.0	38.0
82-83	35.772305602134054	38.0	37.0	38.0	31.0	38.0
84-85	35.768805814477666	38.0	37.0	38.0	31.0	38.0
86-87	35.765062469773554	38.0	37.0	38.0	31.0	38.0
88-89	35.73979159089584	38.0	37.0	38.0	31.0	38.0
90-91	35.72067976590822	38.0	37.0	38.0	31.0	38.0
92-93	35.67078004001148	38.0	36.0	38.0	29.5	38.0
94-95	35.66030867705908	38.0	36.0	38.0	29.5	38.0
96-97	35.686786950831305	38.0	36.0	38.0	31.0	38.0
98-99	35.72335821292866	38.0	36.0	38.0	31.0	38.0
100-101	35.201477339752856	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	7.0
19	30.0
20	131.0
21	817.0
22	3600.0
23	10884.0
24	24741.0
25	45433.0
26	73454.0
27	107870.0
28	148974.0
29	197280.0
30	249426.0
31	311492.0
32	386649.0
33	491272.0
34	658486.0
35	970643.0
36	1872397.0
37	5459426.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.76093989900857	18.541401885206167	14.355217777369372	23.3424404384159
2	14.35229396351389	20.40005764090172	43.15747198337094	22.09017641221344
3	18.134105651320947	26.763814801681875	30.297133273432987	24.804946273564195
4	12.250907176809834	19.426917956057984	38.05926679647069	30.2629080706615
5	14.029827559422092	37.344509583211824	34.95877724449387	13.666885612872221
6	29.268711477957936	38.18035082679009	18.770939433195984	13.779998262055987
7	26.0744811615132	33.2994885232588	22.67213341162859	17.953896903599407
8	23.997510944552594	37.08381166897742	21.02160416954016	17.897073216929826
9	25.39448559626689	17.384370653153685	21.07217162097239	36.14897212960704
10-11	24.026631040933122	27.038799463870607	28.601845834559537	20.332723660636738
12-13	25.031428728904615	24.50626817565729	28.7575843232002	21.704718772237896
14-15	22.168878761879242	25.7662957448611	26.574367069211668	25.49045842404799
16-17	21.554464704618074	29.095075979661516	27.19749354695214	22.152965768768272
18-19	21.6152337239591	27.863378532287214	29.220700093607444	21.30068765014624
20-21	22.424864351844537	26.91679011525076	29.209574016574706	21.44877151632999
22-23	22.118531502078277	27.057089055664836	29.055482302306018	21.76889713995087
24-25	21.96917626800337	27.256379406988458	29.126869523954717	21.647574801053455
26-27	21.833774381419836	27.44122614093371	29.11722703847338	21.607772439173065
28-29	21.645246818512643	27.279483362650414	29.184888263918328	21.89038155491862
30-31	22.273575366817184	27.197278966643406	29.08732567163298	21.44181999490643
32-33	21.989148657862835	27.309586330698327	29.12522819675213	21.57603681468671
34-35	22.002149935857535	27.373666585884855	29.199064332703077	21.425119145554532
36-37	21.977921117056333	27.233392628520047	29.124045096160174	21.664641158263446
38-39	21.670720906217053	27.227437922635257	29.29780743321514	21.804033737932546
40-41	21.906140329893717	27.065493585296707	29.256360764557478	21.7720053202521
42-43	22.03191748196503	27.072351283559787	29.081469294856365	21.814261939618813
44-45	21.80490529749503	27.34729279996983	28.933736529994626	21.914065372540517
46-47	22.09189125410233	27.18338190949514	28.507817384080912	22.216909452321616
48-49	21.963815804002877	27.31545574185168	28.49995131835091	22.22077713579453
50-51	21.810460950524263	27.465211185283227	28.56679445648374	22.15753340770877
52-53	22.005063171615728	27.420079661113007	28.59419000423053	21.98066716304074
54-55	21.919023866025125	27.359015726861536	28.449170663127255	22.272789743986085
56-57	22.096754298301153	27.565995255307413	28.201730250882324	22.135520195509113
58-59	21.799712038897177	27.772677186034933	28.547231750876268	21.880379024191623
60-61	22.05780848180904	27.98464163848264	28.143306095909672	21.81424378379865
62-63	21.781022710772223	28.241757088072745	28.125542836545726	21.851677364609305
64-65	21.74354719096943	28.16688110481509	28.123877475018055	21.96569422919742
66-67	21.835848931430764	28.261163515418964	27.90960941599091	21.993378137159358
68-69	21.815778449643297	28.064472940328617	28.05481857800552	22.064930032022566
70-71	21.884662096992425	27.625841512608307	28.188267980934672	22.301228409464596
72-73	21.955441782988384	27.558268309940832	28.126373153862737	22.359916753208044
74-75	21.88326444527316	27.592265795642767	28.149956874456066	22.374512884628007
76-77	22.0741981631205	27.564321198028903	28.021373215790696	22.34010742305991
78-79	22.096517094224634	27.62134586059194	27.878323239297952	22.403813805885477
80-81	22.119753177704517	27.64241180483572	27.835918698755986	22.40191631870378
82-83	22.10851667421769	27.64869929386078	27.933442862679954	22.309341169241577
84-85	22.216066048949738	27.701604404876058	27.851349468555075	22.230980077619133
86-87	22.101954140938307	27.716412018138982	27.92311004258286	22.258523798339848
88-89	22.143522194526582	27.773284849005584	27.84173042750352	22.241462528964313
90-91	22.21255303526395	27.727097765466453	27.894814530654827	22.165534668614765
92-93	22.26465514161366	27.728453573536655	27.842005669705117	22.164885615144573
94-95	22.216888561567295	27.802168935328247	27.858028892826418	22.12291361027804
96-97	22.184220356078196	27.81716707983134	27.904209455938695	22.094403108151766
98-99	22.383181171545107	28.288982255262297	28.025705968730787	21.302130604461812
100-101	22.924349680267838	30.840518242937137	25.413147151491376	20.821984925303642
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2745.0
1	2641.5
2	3516.5
3	4707.0
4	5807.0
5	8689.0
6	11488.0
7	14376.5
8	17823.0
9	19277.5
10	18053.5
11	16410.0
12	16956.0
13	20355.0
14	28421.5
15	39146.0
16	48335.0
17	55000.5
18	56948.0
19	53448.0
20	47842.5
21	42592.0
22	40037.0
23	42971.0
24	51351.5
25	65147.5
26	84017.5
27	105566.0
28	129989.0
29	155833.5
30	179702.5
31	204594.0
32	230824.0
33	257443.0
34	284680.5
35	309771.5
36	337279.0
37	361780.5
38	379267.5
39	394428.5
40	411242.5
41	426828.5
42	435331.5
43	444136.5
44	455388.0
45	475410.0
46	486724.0
47	479898.5
48	473245.5
49	461717.5
50	445695.0
51	428565.5
52	409067.5
53	386373.5
54	360456.0
55	332819.0
56	303662.5
57	273334.0
58	242618.0
59	210791.0
60	176634.0
61	142972.5
62	111639.5
63	85920.0
64	66049.0
65	49579.5
66	36423.5
67	27544.0
68	21177.5
69	15362.5
70	10838.0
71	7701.5
72	5396.0
73	3618.5
74	2339.0
75	1484.5
76	940.0
77	558.5
78	353.0
79	222.5
80	133.0
81	85.5
82	64.0
83	46.0
84	34.0
85	23.5
86	15.0
87	10.5
88	7.0
89	5.0
90	3.5
91	2.0
92	1.0
93	1.0
94	1.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0025242864963475483
4	0.0
5	0.052147400534258884
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.001044696190992266
28-29	2.6809480923009534E-4
30-31	0.0
32-33	0.0
34-35	1.0969981286126092E-4
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	4.7139124195612094E-6
46-47	9.039630166844454E-5
48-49	0.0
50-51	2.4204444681540424E-5
52-53	9.754500677840252E-6
54-55	0.0010507738335514343
56-57	0.0021563621734904154
58-59	0.008945031906854063
60-61	0.008073593773258397
62-63	0.004107954821220031
64-65	0.004979227461345229
66-67	0.00682502411637783
68-69	0.003822897650779712
70-71	0.005818395744936779
72-73	9.584876842096864E-4
74-75	0.0016056262875082004
76-77	0.001283223965337882
78-79	0.001103724008955175
80-81	5.022381966211199E-5
82-83	0.0
84-85	0.0
86-87	3.0576537342144296E-4
88-89	8.930649826768741E-4
90-91	0.0
92-93	0.0
94-95	1.3337501908929962E-4
96-97	3.38397857190936E-4
98-99	6.364249414425412E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	184.0
20-21	796.0
22-23	1630.0
24-25	1793.0
26-27	3436.0
28-29	7784.0
30-31	21497.0
32-33	28442.0
34-35	38356.0
36-37	59230.0
38-39	67942.0
40-41	70892.0
42-43	80781.0
44-45	96105.0
46-47	97014.0
48-49	87999.0
50-51	80998.0
52-53	67461.0
54-55	72111.0
56-57	73784.0
58-59	86177.0
60-61	87967.0
62-63	86932.0
64-65	98553.0
66-67	108368.0
68-69	107522.0
70-71	119246.0
72-73	89136.0
74-75	90709.0
76-77	95241.0
78-79	99424.0
80-81	101144.0
82-83	97065.0
84-85	95921.0
86-87	99733.0
88-89	103430.0
90-91	107156.0
92-93	106445.0
94-95	113562.0
96-97	177595.0
98-99	915700.0
100-101	7067752.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.79049024929628
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.0213466408861	74.5914227769239
2	7.596966641523359	12.731071186015841
3	1.8483340187882584	4.646184407361606
4	0.7020694650632738	2.3530697866685157
5	0.320153782972864	1.341292121523154
6	0.17310410715297933	0.8702686801508911
7	0.10098901538825422	0.5923343376422884
8	0.059838152717371605	0.40110945214406624
9	0.03904757584430286	0.29446339707366437
>10	0.13360498942652982	1.7382713112493084
>50	0.003442007690734719	0.19389767398029412
>100	9.92006078925479E-4	0.14988497325108024
>500	5.580228493337402E-5	0.02693181955891411
>1k	5.579418206721333E-5	0.06979807645648292
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.816033450609747E-5	0.0	9.080167253048734E-6	9.080167253048734E-6
5	0.0	1.816033450609747E-5	0.0	9.080167253048734E-6	9.080167253048734E-6
6	2.7240501759146202E-5	1.816033450609747E-5	0.0	9.080167253048734E-6	9.080167253048734E-6
7	2.7240501759146202E-5	1.816033450609747E-5	0.0	9.080167253048734E-6	9.080167253048734E-6
8	2.7240501759146202E-5	1.816033450609747E-5	0.0	9.080167253048734E-6	9.080167253048734E-6
9	2.7240501759146202E-5	3.632066901219494E-5	0.0	9.080167253048734E-6	9.080167253048734E-6
10-11	2.7240501759146202E-5	3.632066901219494E-5	0.0	9.080167253048734E-6	9.080167253048734E-6
12-13	2.7240501759146202E-5	3.632066901219494E-5	0.0	1.816033450609747E-5	1.816033450609747E-5
14-15	2.7240501759146202E-5	3.632066901219494E-5	0.0	1.816033450609747E-5	1.816033450609747E-5
16-17	3.632066901219494E-5	3.632066901219494E-5	0.0	2.2700418132621835E-5	1.816033450609747E-5
18-19	3.632066901219494E-5	3.632066901219494E-5	0.0	3.632066901219494E-5	1.816033450609747E-5
20-21	3.632066901219494E-5	3.632066901219494E-5	0.0	4.540083626524367E-5	1.816033450609747E-5
22-23	3.632066901219494E-5	4.994091989176804E-5	0.0	4.540083626524367E-5	2.7240501759146205E-5
24-25	3.632066901219494E-5	5.902108714481677E-5	0.0	6.356117077134114E-5	3.632066901219494E-5
26-27	3.632066901219494E-5	6.356117077134114E-5	0.0	1.4528267604877975E-4	3.632066901219494E-5
28-29	3.632066901219494E-5	6.356117077134114E-5	0.0	5.765906205685946E-4	4.0860752638719304E-5
30-31	3.632066901219494E-5	6.356117077134114E-5	0.0	0.0021974004752377935	4.540083626524367E-5
32-33	3.632066901219494E-5	6.356117077134114E-5	0.0	0.005756826038432897	6.356117077134114E-5
34-35	4.540083626524367E-5	8.626158890396297E-5	0.0	0.010732757693103605	6.356117077134114E-5
36-37	4.540083626524367E-5	9.988183978353607E-5	0.0	0.0179424104920243	6.356117077134114E-5
38-39	6.356117077134114E-5	9.988183978353607E-5	0.0	0.03294284679406081	6.356117077134114E-5
40-41	6.356117077134114E-5	9.988183978353607E-5	0.0	0.08341949655375872	6.356117077134114E-5
42-43	7.264133802438988E-5	1.0442192341006043E-4	0.0	0.09798408482764889	6.356117077134114E-5
44-45	7.718142165091425E-5	1.2712234154268229E-4	0.0	0.11903191252021586	7.264133802438988E-5
46-47	8.172150527743861E-5	1.36202508795731E-4	0.0	0.14302625448639714	9.080167253048734E-5
48-49	8.172150527743861E-5	1.36202508795731E-4	0.0	0.16503657990778728	9.988183978353607E-5
50-51	8.172150527743861E-5	1.36202508795731E-4	0.0	0.18707868591456306	9.988183978353607E-5
52-53	8.172150527743861E-5	1.36202508795731E-4	0.0	0.22860683084638145	9.988183978353607E-5
54-55	8.172150527743861E-5	1.36202508795731E-4	0.0	0.32894267899256996	9.988183978353607E-5
56-57	8.172150527743861E-5	1.36202508795731E-4	0.0	0.44022920884593525	9.988183978353607E-5
58-59	8.172150527743861E-5	1.36202508795731E-4	0.0	0.5567640753715627	9.988183978353607E-5
60-61	8.172150527743861E-5	1.4074259242225536E-4	0.0	0.585520965061968	9.988183978353607E-5
62-63	8.172150527743861E-5	1.4528267604877975E-4	0.0	0.6024509369052774	9.988183978353607E-5
64-65	8.172150527743861E-5	1.5436284330182847E-4	0.0	0.6150042681326173	9.988183978353607E-5
66-67	8.172150527743861E-5	1.5436284330182847E-4	0.0	0.6226588491269374	9.988183978353607E-5
68-69	8.172150527743861E-5	1.5890292692835286E-4	0.0	0.630041025103666	9.988183978353607E-5
70-71	9.534175615701171E-5	1.6344301055487722E-4	0.0	0.6369828129686217	9.988183978353607E-5
72-73	1.1804217428963354E-4	1.6344301055487722E-4	0.0	0.6413367531664587	1.1804217428963354E-4
74-75	1.1804217428963354E-4	1.6798309418140158E-4	0.0	0.6450006006530637	1.1804217428963354E-4
76-77	1.2258225791615793E-4	1.816033450609747E-4	0.0	0.6486417477215363	1.1804217428963354E-4
78-79	1.2712234154268229E-4	1.9068351231402343E-4	0.0	0.6518152661764769	1.1804217428963354E-4
80-81	1.2712234154268229E-4	1.9068351231402343E-4	0.0	0.6531364305117955	1.3166242516920665E-4
82-83	1.2712234154268229E-4	1.9068351231402343E-4	0.0	0.6536903207142315	1.4528267604877975E-4
84-85	1.2712234154268229E-4	1.9068351231402343E-4	0.0	0.6542169704149083	1.4528267604877975E-4
86-87	1.2712234154268229E-4	1.9068351231402343E-4	0.0	0.6543894935927161	1.4528267604877975E-4
88-89	1.2712234154268229E-4	1.9976367956707215E-4	0.0	0.6544439745962345	1.498227596753041E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16225	0.0	53.96767	1
GTATCAA	30865	0.0	39.314045	1
TCAACGC	36605	0.0	32.31523	4
ATCAACG	36955	0.0	31.975784	3
TATCAAC	37800	0.0	31.544865	2
CAACGCA	37645	0.0	31.456678	5
AACGCAG	38890	0.0	30.452765	6
ACGCAGA	43685	0.0	27.110174	7
CGCAGAG	44595	0.0	26.61468	8
GTGGTAT	7330	0.0	24.594248	1
CTTATAC	16525	0.0	24.104311	1
TGGTATC	6845	0.0	23.82859	2
GCAGAGT	51385	0.0	23.097824	9
GTACATG	37350	0.0	21.639536	1
GAGTACT	30650	0.0	21.48207	12-13
TACATGG	37120	0.0	21.368904	2
TATACAC	21325	0.0	20.993433	3
ACATGGG	36800	0.0	20.854889	3
CAGAGTA	50805	0.0	20.195425	10-11
TTATACA	21780	0.0	19.35271	2
>>END_MODULE