FastQCFastQC Report
Fri 27 May 2016
SRR2139713_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139713_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10225189
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157150.053.8832551
GTATCAA290850.038.961571
TCAACGC353200.031.5093544
ATCAACG355300.031.3485893
CAACGCA360650.030.8704015
TATCAAC367300.030.4885752
AACGCAG373700.029.7883056
TGGTATC65100.027.1922722
GTGGTAT71900.026.4775681
ACGCAGA425800.026.1131447
CGCAGAG433150.025.7296628
GCAGAGT499350.022.3272429
GAGTACT289650.020.8806112-13
GTACATG357700.020.8431241
TACATGG358050.020.3295482
CAGAGTA489850.019.99211110-11
ACATGGG354600.019.8714353
AGAGTAC455400.018.99955410-11
GTACTTT320250.018.73120314-15
CATGGGG234450.017.6906474
Produced by FastQC (version 0.11.5)