##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139713_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10225189
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.14016904724206	32.0	28.0	33.0	18.0	33.0
2	30.742794485265748	33.0	31.0	33.0	25.0	34.0
3	31.621701271243005	33.0	32.0	33.0	27.0	34.0
4	30.93355095930256	33.0	31.0	33.0	27.0	34.0
5	31.82563471442924	33.0	32.0	33.0	30.0	34.0
6	35.26447745855847	37.0	35.0	38.0	29.0	38.0
7	35.86297974541106	38.0	36.0	38.0	31.0	38.0
8	36.003829953656606	38.0	37.0	38.0	31.0	38.0
9	36.30454283045526	38.0	37.0	38.0	33.0	38.0
10-11	36.43957260838895	38.0	37.5	38.0	33.5	38.0
12-13	36.48151907020985	38.0	38.0	38.0	34.0	38.0
14-15	36.49193002691686	38.0	38.0	38.0	34.0	38.0
16-17	36.48213460895442	38.0	38.0	38.0	34.0	38.0
18-19	36.49716235073993	38.0	38.0	38.0	34.0	38.0
20-21	36.4915222498077	38.0	38.0	38.0	34.0	38.0
22-23	36.47854178853357	38.0	38.0	38.0	34.0	38.0
24-25	36.46550222130573	38.0	38.0	38.0	34.0	38.0
26-27	36.405809090906914	38.0	38.0	38.0	34.0	38.0
28-29	36.38768446906698	38.0	38.0	38.0	33.5	38.0
30-31	36.39463297542041	38.0	38.0	38.0	34.0	38.0
32-33	36.37722154777188	38.0	38.0	38.0	33.5	38.0
34-35	36.33931892658002	38.0	37.5	38.0	33.0	38.0
36-37	36.317043607005445	38.0	37.0	38.0	33.0	38.0
38-39	36.30346547804291	38.0	37.0	38.0	33.0	38.0
40-41	36.29613865307418	38.0	37.0	38.0	33.0	38.0
42-43	36.24002195828392	38.0	37.0	38.0	33.0	38.0
44-45	36.18564876302018	38.0	37.0	38.0	33.0	38.0
46-47	36.142680485446036	38.0	37.0	38.0	33.0	38.0
48-49	36.090927098283466	38.0	37.0	38.0	33.0	38.0
50-51	36.05970289995033	38.0	37.0	38.0	32.5	38.0
52-53	36.03416874867575	38.0	37.0	38.0	32.0	38.0
54-55	35.984950794214384	38.0	37.0	38.0	31.0	38.0
56-57	35.9808564116679	38.0	37.0	38.0	31.0	38.0
58-59	35.95188046970451	38.0	37.0	38.0	31.0	38.0
60-61	35.93415988867673	38.0	37.0	38.0	31.0	38.0
62-63	35.925874924011254	38.0	37.0	38.0	31.0	38.0
64-65	35.91001158216619	38.0	37.0	38.0	31.0	38.0
66-67	35.91146355302212	38.0	37.0	38.0	31.0	38.0
68-69	35.89291414655694	38.0	37.0	38.0	31.0	38.0
70-71	35.863053705736924	38.0	37.0	38.0	31.0	38.0
72-73	35.832097906809636	38.0	37.0	38.0	31.0	38.0
74-75	35.84647969643055	38.0	37.0	38.0	31.0	38.0
76-77	35.82375319158344	38.0	37.0	38.0	31.0	38.0
78-79	35.80481815194179	38.0	37.0	38.0	31.0	38.0
80-81	35.7580335867529	38.0	37.0	38.0	31.0	38.0
82-83	35.755344420456524	38.0	37.0	38.0	31.0	38.0
84-85	35.75013034277788	38.0	37.0	38.0	31.0	38.0
86-87	35.74129587245828	38.0	37.0	38.0	31.0	38.0
88-89	35.71023715491245	38.0	36.5	38.0	30.5	38.0
90-91	35.69129070457375	38.0	36.0	38.0	30.5	38.0
92-93	35.63828543432038	38.0	36.0	38.0	29.0	38.0
94-95	35.63284943584863	38.0	36.0	38.0	29.0	38.0
96-97	35.66115101355594	38.0	36.0	38.0	30.5	38.0
98-99	35.69035079591106	38.0	36.0	38.0	31.0	38.0
100-101	35.15134179229291	38.0	35.0	38.0	28.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	4.0
19	28.0
20	115.0
21	804.0
22	3528.0
23	10520.0
24	23280.0
25	42480.0
26	67687.0
27	99454.0
28	137900.0
29	181047.0
30	230442.0
31	287775.0
32	359593.0
33	458376.0
34	614641.0
35	914418.0
36	1775677.0
37	5017419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.28953831562429	18.329177093939293	14.38220848533949	22.999076105096933
2	14.48384963837832	20.481733882865147	42.90010678531223	22.134309693444298
3	18.315193531265766	26.693269601563312	30.119794381129196	24.87174248604172
4	12.306188179015566	19.29681690969233	38.26276462958288	30.13423028170922
5	14.292784381534911	37.21618205991561	34.747118132215434	13.743915426334047
6	29.46452138928679	38.13364232191698	18.799007040358862	13.602829248437365
7	26.339366441050625	33.295883332816636	22.76449853396353	17.60025169216921
8	24.15934805703836	37.06948595277799	20.849052276686525	17.92211371349713
9	25.50107386768108	17.497172912891877	21.211959994089106	35.78979322533794
10-11	24.104459096061696	27.104398754878762	28.543125217538766	20.248016931520777
12-13	25.068783569672892	24.604332496934774	28.786587710016903	21.540296223375428
14-15	22.239189037933137	25.874408085484195	26.664608676896272	25.221794199686393
16-17	21.543088347804623	29.096063652221975	27.3290351894718	22.031812810501595
18-19	21.557371702371466	27.992612165897373	29.329110689298748	21.12090544243241
20-21	22.468781595669707	27.064133060300456	29.294272666583083	21.17281267744676
22-23	22.205167599568547	26.958849422320707	29.156450520597982	21.679532457512764
24-25	21.901411964032775	27.141946698700142	29.25983894571265	21.69680239155443
26-27	21.765432243112386	27.292719924180382	29.254062895785644	21.68778493692159
28-29	21.69115953908932	27.253626408950094	29.31648365835924	21.73873039360135
30-31	21.97207175809006	27.097667816021794	29.235304716935005	21.69495570895314
32-33	21.758446771688842	27.287692184287206	29.16086461906381	21.792996424960144
34-35	21.917688864061706	27.342985119458856	29.133028177545363	21.606297838934076
36-37	21.931735488546682	27.194541725177185	29.10826878129021	21.765454004985923
38-39	21.751907298228762	27.338005889285256	29.209509971070457	21.70057684141553
40-41	21.99533285298487	27.218007367232065	29.079015989003654	21.70764379077941
42-43	21.954401571330802	27.250315038216787	28.911574794486572	21.88370859596584
44-45	21.943561630828174	27.42367844086639	28.761240825648056	21.871519102657384
46-47	22.076372797558154	27.368109153396396	28.432460309738026	22.123057739307416
48-49	21.967087344290658	27.48916016558876	28.445340652626918	22.09841183749367
50-51	21.9303005132176	27.667840914307888	28.35126178615631	22.050596786318195
52-53	22.14533352725174	27.58104857918241	28.226405752036587	22.04721214152926
54-55	22.083620781220535	27.501092249180203	28.252822585157954	22.162464384441304
56-57	22.04498052324026	27.615576344334684	28.242945119672587	22.096498012752466
58-59	21.994467156702573	27.618334581149657	28.331144294735072	22.056053967412698
60-61	22.07263069446693	27.609220496635057	28.297911845237145	22.02023696366087
62-63	21.990804035149917	27.714255271547472	28.26309190541994	22.03184878788267
64-65	21.964374366452013	27.675640136940338	28.227765907158286	22.132219589449363
66-67	22.05580181152762	27.75571782189473	28.046332155097677	22.142148211479974
68-69	21.97485760997521	27.734679337244245	28.15711964121493	22.133343411565612
70-71	21.999644244753956	27.444758251867363	28.245484693899193	22.31011280947949
72-73	22.04156688876085	27.49237456081873	28.138760063526625	22.327298486893792
74-75	21.89226458431096	27.58591391779731	28.154733560837364	22.36708793705436
76-77	22.10187453981576	27.490136903337724	28.052901013661003	22.355087543185515
78-79	22.08805521405183	27.556025281213543	27.927907108164096	22.428012396570534
80-81	22.117287702123633	27.60611267802549	27.873284656745888	22.403314963104993
82-83	22.147603223915635	27.565556532077295	27.98443749583937	22.302402748167697
84-85	22.213724947026602	27.556438835069102	27.935204016865768	22.29463220103853
86-87	22.069237955909852	27.629919803628173	27.97086697961116	22.32997526085082
88-89	22.143290150985667	27.608928936996648	27.98496119198446	22.262819720033228
90-91	22.13921511086265	27.597873447693367	27.97729914141741	22.28561230002657
92-93	22.125369676040428	27.555015226320467	28.01398216324429	22.305632934394815
94-95	22.03577283663663	27.6536849878451	28.004091259204028	22.306450916314233
96-97	22.063135395292576	27.667476784578408	28.022098707280602	22.247289112848414
98-99	22.261818641276243	28.2785342261955	28.071067559024865	21.388579573503392
100-101	22.919986435178508	30.78962970907385	25.401366905007734	20.88901695073991
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2937.0
1	2667.0
2	3168.0
3	4108.5
4	4842.5
5	6959.0
6	9165.5
7	11928.0
8	15510.0
9	17186.5
10	16150.5
11	14564.0
12	15041.5
13	18096.0
14	25122.0
15	34390.0
16	42491.5
17	48559.0
18	50694.0
19	48290.0
20	43700.0
21	39243.5
22	37380.5
23	40270.0
24	48047.5
25	61016.5
26	78545.0
27	98219.5
28	119923.0
29	142716.5
30	164782.0
31	187703.0
32	213487.0
33	240721.5
34	266391.0
35	289896.5
36	316598.5
37	340294.0
38	356153.5
39	370360.5
40	386917.0
41	401240.0
42	408329.5
43	415472.5
44	425414.5
45	431385.0
46	431018.5
47	427206.0
48	422774.5
49	417130.0
50	408361.0
51	395200.5
52	376772.0
53	355812.5
54	333529.5
55	310261.0
56	284049.5
57	255923.5
58	229431.0
59	200231.5
60	167628.5
61	135809.0
62	106900.0
63	82663.0
64	62595.5
65	46525.0
66	34326.0
67	26389.0
68	20278.0
69	14964.0
70	10879.5
71	7601.0
72	5168.5
73	3420.0
74	2265.0
75	1432.5
76	885.5
77	536.5
78	337.5
79	217.0
80	143.0
81	97.5
82	67.5
83	43.0
84	24.0
85	16.5
86	14.5
87	10.0
88	6.5
89	4.0
90	2.5
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.00246450212313924
4	0.0
5	0.04969101304631142
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	4.8898851649588095E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	9.490571093158712E-4
28-29	2.349172481969734E-4
30-31	0.0
32-33	0.0
34-35	4.92255322399916E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	1.0144690679758186E-5
46-47	9.724953739930579E-5
48-49	0.0
50-51	2.0820604569976957E-5
52-53	1.5728779634921393E-5
54-55	0.0011719501027990286
56-57	0.0021540505697676504
58-59	0.008773608684940034
60-61	0.0081883836292862
62-63	0.004261801716459716
64-65	0.005253302024532481
66-67	0.006775939315973917
68-69	0.0038631731264240207
70-71	0.005706463585183871
72-73	9.594349202444023E-4
74-75	0.00159088510449089
76-77	0.0013036746572805194
78-79	0.0011525434133996225
80-81	7.727568725578282E-5
82-83	0.0
84-85	0.0
86-87	2.1475975041480844E-4
88-89	0.0010549170583873634
90-91	0.0
92-93	6.357146526124566E-6
94-95	1.480379183036657E-4
96-97	3.7870072222145326E-4
98-99	2.0248548229713298E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	160.0
20-21	693.0
22-23	1378.0
24-25	1656.0
26-27	3414.0
28-29	7236.0
30-31	19488.0
32-33	26052.0
34-35	34961.0
36-37	54030.0
38-39	60853.0
40-41	63524.0
42-43	73152.0
44-45	87398.0
46-47	87626.0
48-49	79399.0
50-51	71751.0
52-53	64011.0
54-55	69507.0
56-57	70807.0
58-59	76954.0
60-61	78318.0
62-63	74333.0
64-65	80317.0
66-67	86044.0
68-69	87980.0
70-71	88716.0
72-73	80966.0
74-75	81918.0
76-77	87090.0
78-79	90971.0
80-81	90706.0
82-83	86296.0
84-85	86646.0
86-87	90032.0
88-89	94532.0
90-91	97418.0
92-93	95943.0
94-95	103430.0
96-97	163365.0
98-99	856799.0
100-101	6669319.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.0986608901537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.63804556537701	78.03814348879695
2	6.606788246684772	11.376712416487306
3	1.5301706649541575	3.9523693555784702
4	0.5526569197670145	1.9033208289446828
5	0.2587457686495752	1.1138832095860969
6	0.13594593650759468	0.7022857852057056
7	0.07840651103383384	0.4725486923557508
8	0.04964763594958493	0.3419675977296893
9	0.033424281399251014	0.25900072827220666
>10	0.11296203092339167	1.5192986026777675
>50	0.002593614854601672	0.1510677135368911
>100	5.039063732346158E-4	0.07229720950134717
>500	5.4460432903703055E-5	0.028790263145538612
>1k	5.4457093088193225E-5	0.06831410818159055
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.779770329917619E-6	9.779770329917619E-6
2	0.0	0.0	0.0	9.779770329917619E-6	9.779770329917619E-6
3	0.0	1.9559540659835238E-5	0.0	9.779770329917619E-6	9.779770329917619E-6
4	0.0	4.889885164958809E-5	0.0	9.779770329917619E-6	9.779770329917619E-6
5	0.0	4.889885164958809E-5	0.0	9.779770329917619E-6	9.779770329917619E-6
6	2.9339310989752855E-5	4.889885164958809E-5	0.0	9.779770329917619E-6	9.779770329917619E-6
7	2.9339310989752855E-5	4.889885164958809E-5	0.0	1.9559540659835238E-5	9.779770329917619E-6
8	2.9339310989752855E-5	8.801793296925857E-5	0.0	1.9559540659835238E-5	3.9119081319670476E-5
9	2.9339310989752855E-5	1.2713701428892905E-4	0.0	4.889885164958809E-5	3.9119081319670476E-5
10-11	2.9339310989752855E-5	1.2713701428892905E-4	0.0	4.889885164958809E-5	3.9119081319670476E-5
12-13	2.9339310989752855E-5	1.2713701428892905E-4	0.0	6.356850714446451E-5	4.400896648462928E-5
14-15	2.9339310989752855E-5	1.7114598077355833E-4	0.0	8.312804780429975E-5	6.845839230942332E-5
16-17	3.9119081319670476E-5	1.8581563626843476E-4	0.0	9.779770329917618E-5	9.779770329917618E-5
18-19	3.9119081319670476E-5	2.0048529176331119E-4	0.0	1.0757747362909379E-4	1.1246735879405261E-4
20-21	3.9119081319670476E-5	2.1515494725818759E-4	0.0	1.2224712912397022E-4	1.1735724395901142E-4
22-23	3.9119081319670476E-5	2.4449425824794044E-4	0.0	1.3202689945388785E-4	1.3691678461884668E-4
24-25	3.9119081319670476E-5	2.8361333956761095E-4	0.0	1.8581563626843473E-4	1.7114598077355833E-4
26-27	3.9119081319670476E-5	2.9339310989752855E-4	0.0	2.738335692376933E-4	1.7603586593851713E-4
28-29	3.9119081319670476E-5	2.9339310989752855E-4	0.0	7.188131192489449E-4	1.8092575110347593E-4
30-31	3.9119081319670476E-5	3.4229196154711666E-4	0.0	0.0024449425824794044	1.9070552143339356E-4
32-33	3.9119081319670476E-5	3.5207173187703426E-4	0.0	0.005936320590259994	1.9559540659835236E-4
34-35	3.9119081319670476E-5	3.6674138737191066E-4	0.0	0.010576821611805905	1.9559540659835236E-4
36-37	3.9119081319670476E-5	3.911908131967047E-4	0.0	0.018239271665296358	1.9559540659835236E-4
38-39	3.9119081319670476E-5	4.1075035385653997E-4	0.0	0.03183315242388185	1.9559540659835236E-4
40-41	4.889885164958809E-5	4.3030989451637517E-4	0.0	0.067191912051699	1.9559540659835236E-4
42-43	4.889885164958809E-5	4.596492055061281E-4	0.0	0.07933349691629171	1.9559540659835236E-4
44-45	4.889885164958809E-5	5.232177126505926E-4	0.0	0.09657523200793648	1.9559540659835236E-4
46-47	4.889885164958809E-5	5.770064494651395E-4	0.0	0.11656997244745305	2.0537517692826999E-4
48-49	4.889885164958809E-5	5.818963346300983E-4	0.0	0.13480924411274942	2.0537517692826999E-4
50-51	4.889885164958809E-5	6.063457604548923E-4	0.0	0.1522710240368173	2.1515494725818759E-4
52-53	4.889885164958809E-5	6.063457604548923E-4	0.0	0.17999667292213378	2.200448324231464E-4
54-55	4.889885164958809E-5	6.307951862796863E-4	0.0	0.24253341429679198	2.2982460275306404E-4
56-57	4.889885164958809E-5	6.65024382434398E-4	0.0	0.30990136221442943	2.3471448791802284E-4
58-59	4.889885164958809E-5	6.943636934241509E-4	0.0	0.3848779714487429	2.3471448791802284E-4
60-61	4.889885164958809E-5	7.090333489190273E-4	0.0	0.4101977968328996	2.3471448791802284E-4
62-63	5.867862197950571E-5	7.237030044139037E-4	0.0	0.42240784008980176	2.542740285778581E-4
64-65	5.867862197950571E-5	8.019411670532446E-4	0.0	0.4343196003516414	2.542740285778581E-4
66-67	5.867862197950571E-5	8.019411670532446E-4	0.0	0.4412436777452231	2.542740285778581E-4
68-69	5.867862197950571E-5	8.263905928780387E-4	0.0	0.44742938247889597	2.640537989077757E-4
70-71	6.356850714446451E-5	8.312804780429975E-4	0.0	0.4531994469735474	2.738335692376933E-4
72-73	6.845839230942332E-5	8.312804780429975E-4	0.0	0.4578399479950933	3.32512191217199E-4
74-75	6.845839230942332E-5	8.459501335378739E-4	0.0	0.46163938876826627	3.32512191217199E-4
76-77	6.845839230942332E-5	8.606197890327503E-4	0.0	0.46497918033593316	3.32512191217199E-4
78-79	6.845839230942332E-5	8.70399559362668E-4	0.0	0.46798156982721784	3.32512191217199E-4
80-81	6.845839230942332E-5	8.70399559362668E-4	0.0	0.4691795916926328	3.4229196154711666E-4
82-83	6.845839230942332E-5	8.70399559362668E-4	0.0	0.46961968135747906	3.6185150220695186E-4
84-85	6.845839230942332E-5	8.801793296925857E-4	0.0	0.47019668780694424	3.6185150220695186E-4
86-87	6.845839230942332E-5	8.997388703524209E-4	0.0	0.47042651240969724	3.765211577018283E-4
88-89	6.845839230942332E-5	9.388579516720914E-4	0.0	0.4704851910316768	3.814110428667871E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15715	0.0	53.883255	1
GTATCAA	29085	0.0	38.96157	1
TCAACGC	35320	0.0	31.509354	4
ATCAACG	35530	0.0	31.348589	3
CAACGCA	36065	0.0	30.870401	5
TATCAAC	36730	0.0	30.488575	2
AACGCAG	37370	0.0	29.788305	6
TGGTATC	6510	0.0	27.192272	2
GTGGTAT	7190	0.0	26.477568	1
ACGCAGA	42580	0.0	26.113144	7
CGCAGAG	43315	0.0	25.729662	8
GCAGAGT	49935	0.0	22.327242	9
GAGTACT	28965	0.0	20.88061	12-13
GTACATG	35770	0.0	20.843124	1
TACATGG	35805	0.0	20.329548	2
CAGAGTA	48985	0.0	19.992111	10-11
ACATGGG	35460	0.0	19.871435	3
AGAGTAC	45540	0.0	18.999554	10-11
GTACTTT	32025	0.0	18.731203	14-15
CATGGGG	23445	0.0	17.690647	4
>>END_MODULE