FastQCFastQC Report
Fri 27 May 2016
SRR2139712_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139712_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9164945
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155100.053.8239561
GTATCAA293250.039.184341
CTTATAC92600.039.0613861
ATCAACG361150.031.3481643
TCAACGC361600.031.2848744
TATACAC127600.031.0357113
TATCAAC368750.030.8858472
CAACGCA370500.030.5790845
AACGCAG379200.029.864636
TGGTATC63900.027.7724342
GTGGTAT66950.027.7092191
TTATACA142800.026.7534072
ACGCAGA428550.026.3959127
CGCAGAG432100.026.2084438
TACACAT167000.023.7128665
ACACATC159400.023.4251296
GCAGAGT489450.023.111599
ACATCTC165750.022.7575688
GTACATG364300.021.4272751
GAGTACT278700.021.33469612-13
Produced by FastQC (version 0.11.5)