##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139712_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9164945
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.97513482077634	32.0	27.0	33.0	18.0	33.0
2	30.678915258083926	33.0	31.0	33.0	25.0	33.0
3	31.593750971773424	33.0	32.0	33.0	27.0	34.0
4	30.887587541441874	33.0	31.0	33.0	27.0	34.0
5	31.811958391457885	33.0	32.0	33.0	30.0	34.0
6	35.248193742570194	37.0	35.0	38.0	29.0	38.0
7	35.86847962535509	38.0	36.0	38.0	31.0	38.0
8	36.0204403845304	38.0	37.0	38.0	31.0	38.0
9	36.31499938079279	38.0	37.0	38.0	33.0	38.0
10-11	36.44886592336343	38.0	37.5	38.0	33.5	38.0
12-13	36.48842180722306	38.0	38.0	38.0	34.0	38.0
14-15	36.49621334334249	38.0	38.0	38.0	34.0	38.0
16-17	36.49168216503209	38.0	38.0	38.0	34.0	38.0
18-19	36.511803889712375	38.0	38.0	38.0	34.0	38.0
20-21	36.5073396878405	38.0	38.0	38.0	34.0	38.0
22-23	36.49469817510578	38.0	38.0	38.0	34.0	38.0
24-25	36.47840735768065	38.0	38.0	38.0	34.0	38.0
26-27	36.42380326675571	38.0	38.0	38.0	34.0	38.0
28-29	36.407863801978884	38.0	38.0	38.0	34.0	38.0
30-31	36.406676235618846	38.0	38.0	38.0	34.0	38.0
32-33	36.38388463782262	38.0	38.0	38.0	33.5	38.0
34-35	36.332567294061356	38.0	38.0	38.0	33.0	38.0
36-37	36.30514129072962	38.0	37.0	38.0	33.0	38.0
38-39	36.26069814535602	38.0	37.0	38.0	33.0	38.0
40-41	36.29949059657237	38.0	37.0	38.0	33.0	38.0
42-43	36.25540808279453	38.0	37.0	38.0	33.0	38.0
44-45	36.20238387198691	38.0	37.0	38.0	33.0	38.0
46-47	36.160773628361184	38.0	37.0	38.0	33.0	38.0
48-49	36.111306810844795	38.0	37.0	38.0	33.0	38.0
50-51	36.084649235845205	38.0	37.0	38.0	33.0	38.0
52-53	36.07745607522938	38.0	37.0	38.0	32.5	38.0
54-55	36.03320980130684	38.0	37.0	38.0	31.5	38.0
56-57	36.03029547361046	38.0	37.0	38.0	31.5	38.0
58-59	36.00127862872566	38.0	37.0	38.0	31.0	38.0
60-61	35.98736057693749	38.0	37.0	38.0	31.0	38.0
62-63	35.98443631653369	38.0	37.0	38.0	31.0	38.0
64-65	35.97382001709502	38.0	37.0	38.0	31.0	38.0
66-67	35.97407114583616	38.0	37.0	38.0	31.0	38.0
68-69	35.95246681548993	38.0	37.0	38.0	31.0	38.0
70-71	35.92231345792506	38.0	37.0	38.0	31.0	38.0
72-73	35.89003199457121	38.0	37.0	38.0	31.0	38.0
74-75	35.90905150294678	38.0	37.0	38.0	31.0	38.0
76-77	35.89118008740297	38.0	37.0	38.0	31.0	38.0
78-79	35.8767800341852	38.0	37.0	38.0	31.0	38.0
80-81	35.836598267678504	38.0	37.0	38.0	31.0	38.0
82-83	35.8386311981349	38.0	37.0	38.0	31.0	38.0
84-85	35.83284607794639	38.0	37.0	38.0	31.0	38.0
86-87	35.82612912420137	38.0	37.0	38.0	31.0	38.0
88-89	35.80000489930575	38.0	37.0	38.0	31.0	38.0
90-91	35.78070207511581	38.0	37.0	38.0	31.0	38.0
92-93	35.72949056631013	38.0	36.5	38.0	31.0	38.0
94-95	35.72279440422693	38.0	36.0	38.0	31.0	38.0
96-97	35.7550465269498	38.0	36.0	38.0	31.0	38.0
98-99	35.790205832692095	38.0	36.0	38.0	31.0	38.0
100-101	35.25780869480242	38.0	35.5	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	17.0
19	41.0
20	149.0
21	766.0
22	3213.0
23	9031.0
24	20308.0
25	37112.0
26	59911.0
27	88093.0
28	121411.0
29	160894.0
30	203755.0
31	254525.0
32	317487.0
33	405888.0
34	546424.0
35	815701.0
36	1598400.0
37	4521817.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.22556818398801	18.638093300068903	14.437533449464235	22.69880506647885
2	14.266141258894624	20.50531672585051	43.421635372607255	21.80690664264761
3	17.853748244220153	27.304947630214134	30.646780576538475	24.194523549027245
4	12.040945144788102	19.66916331740125	38.39087959611323	29.899011941697413
5	13.813065058051327	37.59105282313695	35.050066907982256	13.545815210829465
6	28.740379784057623	38.63950083715723	18.883888555796023	13.736230822989118
7	25.799532894087196	33.65404811485503	22.848385887749462	17.698033103308312
8	24.04833853340091	36.975573775947375	21.088582637429905	17.887505053221815
9	25.303217858917865	17.41661297476417	21.257683488553393	36.02248567776457
10-11	23.83265802467991	27.24133641827638	28.71719361109096	20.20881194595276
12-13	25.00813152724866	24.705822020754077	28.885263359463696	21.400783092533562
14-15	22.200100491601425	25.968300955433993	26.71931473674965	25.112283816214937
16-17	21.326767372853848	29.42162227923899	27.288292510211466	21.963317837695698
18-19	21.357536788273144	27.98155362634473	29.55766783106718	21.103241754314947
20-21	22.287177332909213	27.01353885407572	29.47514273289392	21.224141080121147
22-23	22.009294869065453	27.155863521241752	29.371779696231066	21.46306191346173
24-25	21.752292585593384	27.423305959125376	29.544356138414546	21.28004531686669
26-27	21.686580679268147	27.461508682761494	29.559839296794287	21.29207134117607
28-29	21.47372243957144	27.240802039382615	29.767476666329216	21.517998854716726
30-31	21.97264917758295	27.30901717277543	29.397133562691963	21.321200086949656
32-33	21.7657879544118	27.30546526892296	29.406487171603697	21.522259605061546
34-35	21.555347644274416	27.542928309288605	29.68213795234007	21.219586094096908
36-37	21.817198250606985	27.31902070088525	29.418371944418087	21.44540910408968
38-39	21.456567654227168	27.35008423276885	29.579507368676722	21.613840744327263
40-41	21.7206008058223	27.221510764329267	29.51647634825444	21.54141208159399
42-43	21.870309168127378	27.256706493465416	29.269435367654474	21.603548970752733
44-45	21.663855310249456	27.621199247769585	29.044023510648344	21.670921931332618
46-47	22.078661498933545	27.31250549116744	28.532658729139754	22.07617428075926
48-49	21.883901397498146	27.47049224435601	28.53162532333234	22.11398103481351
50-51	21.64589247727236	27.640272866678533	28.667372120295763	22.046462535753346
52-53	21.98578890505741	27.656742776949034	28.491581667127026	21.865886650866535
54-55	21.936338288655293	27.600355641851102	28.255117649957157	22.20818841953645
56-57	22.130592151104263	27.908403031445804	27.938865846967452	22.02213897048248
58-59	21.76198248793865	28.1849498279424	28.30984868529653	21.74321899882242
60-61	22.03357365353698	28.487148853713514	27.808382195321858	21.670895297427652
62-63	21.792045972833535	28.766373340569835	27.73998269355725	21.701597993039385
64-65	21.738218900462503	28.674366196900277	27.79795309498123	21.78946180765599
66-67	21.87857052529643	28.648916241770124	27.619697602383635	21.85281563054981
68-69	21.816608479716155	28.406705423704746	27.888056208947525	21.888629887631573
70-71	21.957937594963372	27.86773838472004	28.037442631298106	22.13688138901848
72-73	22.080060333833586	27.793414633261992	27.967883045184124	22.158641987720298
74-75	21.837436410195306	28.00781949050366	27.979374202328117	22.17536989697291
76-77	22.041806473428885	27.91927692386803	27.915056808009364	22.123859794693722
78-79	22.00826680114109	27.99667797897138	27.816862224268952	22.178192995618577
80-81	22.037292943721052	27.98742140530534	27.7831087737833	22.192176877190313
82-83	22.114770594639108	27.868325775425713	27.921096043770056	22.09580758616513
84-85	22.20890540099538	27.86277706471228	27.812754404031136	22.115563130261208
86-87	21.974737730702156	27.957215560622462	27.908875589455327	22.15917111922006
88-89	22.072246836429642	27.974655352983902	27.804980422178883	22.148117388407574
90-91	22.177246573498692	27.939981659624834	27.867931752397773	22.014840014478704
92-93	22.159386451083783	27.96977015777952	27.746435233872507	22.124408157264188
94-95	21.97794167403754	28.042099947344273	27.795186571084745	22.18477180753344
96-97	22.087639682422807	28.024307481747513	27.83260167892544	22.055451156904237
98-99	22.24732201815488	28.648106068632302	27.88126695486708	21.223304958345743
100-101	22.821390876350645	31.126896320325066	25.31915952273911	20.732553280585176
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3597.0
1	3039.0
2	2771.5
3	3532.5
4	4903.0
5	7529.0
6	9768.5
7	10267.5
8	9743.5
9	9130.5
10	8740.5
11	9239.5
12	11197.0
13	17330.5
14	27780.0
15	40453.5
16	52272.0
17	59503.5
18	62753.5
19	58922.5
20	52312.5
21	47357.0
22	44696.0
23	47495.5
24	55396.0
25	69622.5
26	88896.5
27	108693.5
28	128409.0
29	148977.0
30	167480.0
31	186327.0
32	208490.5
33	231718.0
34	253649.5
35	273165.5
36	296318.5
37	317691.5
38	329443.0
39	336450.0
40	346403.5
41	356042.0
42	360141.0
43	368909.0
44	393456.0
45	405802.0
46	390045.0
47	375673.5
48	368675.5
49	360333.5
50	350690.5
51	339979.0
52	324723.0
53	306181.0
54	288663.0
55	270065.5
56	249006.5
57	225710.5
58	203201.5
59	180049.5
60	152915.5
61	125459.5
62	98818.5
63	75652.5
64	57434.0
65	42742.0
66	31221.0
67	23628.5
68	18156.5
69	13292.5
70	9626.0
71	6970.0
72	4977.5
73	3432.0
74	2316.5
75	1576.0
76	1065.5
77	688.0
78	451.5
79	290.5
80	188.5
81	123.5
82	84.5
83	61.5
84	45.5
85	30.5
86	18.5
87	12.5
88	9.5
89	5.5
90	3.5
91	2.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.002334984006996223
4	0.0
5	0.05022397843085801
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0011580267944458194
28-29	2.8970903756088607E-4
30-31	0.0
32-33	0.0
34-35	1.0459164476802042E-4
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	1.709666098791574E-5
46-47	9.211910678089897E-5
48-49	0.0
50-51	3.520616849625301E-5
52-53	1.1875036144641265E-5
54-55	0.0010057778946661983
56-57	0.002102089422521605
58-59	0.008963229357637058
60-61	0.007794160290046192
62-63	0.004220147506275061
64-65	0.005076341843746182
66-67	0.0065985028631474945
68-69	0.0035845602582887655
70-71	0.005631965080514312
72-73	0.001021814756191703
74-75	0.0015983985112516265
76-77	0.0013595722704870378
78-79	0.0010889968372128733
80-81	6.887092658600275E-5
82-83	0.0
84-85	0.0
86-87	2.210229784757129E-4
88-89	9.453960188146797E-4
90-91	0.0
92-93	0.0
94-95	1.5020866612376247E-4
96-97	2.1342097821505364E-4
98-99	2.363115733435504E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1122.0
20-21	3803.0
22-23	2502.0
24-25	3029.0
26-27	5155.0
28-29	10198.0
30-31	21172.0
32-33	26872.0
34-35	36107.0
36-37	54822.0
38-39	65180.0
40-41	64729.0
42-43	74979.0
44-45	88211.0
46-47	87848.0
48-49	79990.0
50-51	101441.0
52-53	68258.0
54-55	72917.0
56-57	76876.0
58-59	78348.0
60-61	77741.0
62-63	75139.0
64-65	86289.0
66-67	95859.0
68-69	95096.0
70-71	107341.0
72-73	77325.0
74-75	77696.0
76-77	81444.0
78-79	85532.0
80-81	86989.0
82-83	81175.0
84-85	80475.0
86-87	83311.0
88-89	88002.0
90-91	93166.0
92-93	88337.0
94-95	93443.0
96-97	140288.0
98-99	726985.0
100-101	5719753.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.29159721980443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.3047839460359	75.27642878180914
2	7.373839840905861	12.431054756659671
3	1.8435878943243402	4.66196904683084
4	0.6971319288980576	2.350494550389617
5	0.3098451914955195	1.3058673041016753
6	0.16291138938064872	0.8239236729715419
7	0.08572153085074354	0.505792332607514
8	0.05845286301581969	0.39416681485391003
9	0.038936580180317044	0.2953823880308314
>10	0.12117725709720673	1.5789274850391797
>50	0.0026153786093357197	0.15191427541269892
>100	9.311828369121625E-4	0.13889595998407245
>500	2.1672143163433272E-5	0.013244151827559905
>1k	4.334422626399021E-5	0.0719384794817093
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0911140219608519E-5	0.0	0.0	0.0
3	0.0	1.0911140219608519E-5	0.0	0.0	0.0
4	0.0	7.637798153725964E-5	0.0	0.0	0.0
5	0.0	7.637798153725964E-5	0.0	0.0	0.0
6	1.0911140219608519E-5	7.637798153725964E-5	0.0	0.0	0.0
7	1.0911140219608519E-5	7.637798153725964E-5	0.0	0.0	0.0
8	1.0911140219608519E-5	7.637798153725964E-5	0.0	1.0911140219608519E-5	1.0911140219608519E-5
9	1.0911140219608519E-5	1.745782435137363E-4	0.0	3.2733420658825556E-5	1.0911140219608519E-5
10-11	1.0911140219608519E-5	1.745782435137363E-4	0.0	6.001127120784685E-5	1.0911140219608519E-5
12-13	1.0911140219608519E-5	1.745782435137363E-4	0.0	8.728912175686815E-5	1.0911140219608519E-5
14-15	2.1822280439217037E-5	3.0551192614903855E-4	0.0	8.728912175686815E-5	1.6366710329412778E-5
16-17	3.2733420658825556E-5	3.0551192614903855E-4	0.0	1.0365583208628092E-4	2.1822280439217037E-5
18-19	3.2733420658825556E-5	3.218786364784513E-4	0.0	1.2002254241569371E-4	2.1822280439217037E-5
20-21	3.2733420658825556E-5	3.491564870274726E-4	0.0	1.2547811252549797E-4	2.7277850549021296E-5
22-23	3.2733420658825556E-5	3.818899076862982E-4	0.0	1.3093368263530222E-4	3.818899076862981E-5
24-25	3.2733420658825556E-5	3.928010479059067E-4	0.0	1.3093368263530222E-4	4.3644560878434074E-5
26-27	3.2733420658825556E-5	4.037121881255152E-4	0.0	1.8548938373334485E-4	4.3644560878434074E-5
28-29	5.455570109804259E-5	4.0916775823531947E-4	0.0	4.41901178894145E-4	4.3644560878434074E-5
30-31	5.455570109804259E-5	4.3644560878434074E-4	0.0	0.001200225424156937	4.3644560878434074E-5
32-33	6.001127120784685E-5	4.3644560878434074E-4	0.0	0.0030114747006119514	5.455570109804259E-5
34-35	7.092241142745537E-5	4.800901696627749E-4	0.0	0.005488303530463085	5.455570109804259E-5
36-37	7.637798153725964E-5	5.128235903216004E-4	0.0	0.008968957260518204	5.455570109804259E-5
38-39	7.637798153725964E-5	5.346458707608174E-4	0.0	0.013982626191428317	5.455570109804259E-5
40-41	7.637798153725964E-5	5.8920157185886E-4	0.0	0.019629141255075727	5.455570109804259E-5
42-43	7.637798153725964E-5	6.001127120784685E-4	0.0	0.025155633776307442	5.455570109804259E-5
44-45	8.728912175686815E-5	6.601239832863154E-4	0.0	0.03132588357049606	5.455570109804259E-5
46-47	1.0365583208628092E-4	7.092241142745537E-4	0.0	0.03799259024467687	5.455570109804259E-5
48-49	1.1456697230588945E-4	7.092241142745537E-4	0.0	0.044424707404136086	5.455570109804259E-5
50-51	1.2002254241569371E-4	8.456133670196603E-4	0.0	0.051680615650175746	5.455570109804259E-5
52-53	1.2002254241569371E-4	8.674356474588773E-4	0.0	0.05833095561402714	8.183355164706389E-5
54-55	1.2002254241569371E-4	9.056246382275071E-4	0.0	0.06509586255018443	1.1456697230588945E-4
56-57	1.2002254241569371E-4	9.383580588863327E-4	0.0	0.07208444786084368	1.2547811252549797E-4
58-59	1.2002254241569371E-4	9.492691991059411E-4	0.0	0.07904029975084412	1.3093368263530222E-4
60-61	1.2547811252549797E-4	9.87458189874571E-4	0.0	0.08570700642502492	1.3093368263530222E-4
62-63	1.4184482285491075E-4	9.929137599843753E-4	0.0	0.09218822371547239	1.4184482285491075E-4
64-65	1.4184482285491075E-4	0.001091114021960852	0.0	0.09824390653735511	1.4184482285491075E-4
66-67	1.4184482285491075E-4	0.0011020251621804604	0.0	0.10426685593857901	1.4730039296471502E-4
68-69	1.4184482285491075E-4	0.0011183918725098732	0.0	0.110398916741999	1.5275596307451927E-4
70-71	1.4184482285491075E-4	0.0011238474426196775	0.0	0.11617636548828171	1.5275596307451927E-4
72-73	1.745782435137363E-4	0.0011293030127294819	0.0	0.12066629968865061	1.636671032941278E-4
74-75	1.745782435137363E-4	0.0011674920034981115	0.0	0.1243597206529881	1.636671032941278E-4
76-77	1.8003381362354057E-4	0.0012165921344863499	0.0	0.12775308526128637	1.6912267340393205E-4
78-79	1.8548938373334482E-4	0.0012547811252549797	0.0	0.1307318265412395	1.745782435137363E-4
80-81	1.8548938373334482E-4	0.0012547811252549797	0.0	0.1320466189377023	1.8003381362354057E-4
82-83	1.9094495384314907E-4	0.0012547811252549797	0.0	0.13249397568670623	1.8548938373334482E-4
84-85	1.9640052395295335E-4	0.0012547811252549797	0.0	0.13288132116450235	1.8548938373334482E-4
86-87	1.9640052395295335E-4	0.0012766034056941968	0.0	0.13315955524010237	1.8548938373334482E-4
88-89	1.9640052395295335E-4	0.001303881256243218	0.0	0.13324684436185924	1.8548938373334482E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15510	0.0	53.823956	1
GTATCAA	29325	0.0	39.18434	1
CTTATAC	9260	0.0	39.061386	1
ATCAACG	36115	0.0	31.348164	3
TCAACGC	36160	0.0	31.284874	4
TATACAC	12760	0.0	31.035711	3
TATCAAC	36875	0.0	30.885847	2
CAACGCA	37050	0.0	30.579084	5
AACGCAG	37920	0.0	29.86463	6
TGGTATC	6390	0.0	27.772434	2
GTGGTAT	6695	0.0	27.709219	1
TTATACA	14280	0.0	26.753407	2
ACGCAGA	42855	0.0	26.395912	7
CGCAGAG	43210	0.0	26.208443	8
TACACAT	16700	0.0	23.712866	5
ACACATC	15940	0.0	23.425129	6
GCAGAGT	48945	0.0	23.11159	9
ACATCTC	16575	0.0	22.757568	8
GTACATG	36430	0.0	21.427275	1
GAGTACT	27870	0.0	21.334696	12-13
>>END_MODULE