FastQCFastQC Report
Fri 27 May 2016
SRR2139711_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139711_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9802728
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147950.054.846891
GTATCAA280550.038.993011
TCAACGC337000.031.9265044
ATCAACG339400.031.7266463
TATCAAC347850.031.1654382
CAACGCA347150.031.017735
AACGCAG356300.030.2649576
ACGCAGA399550.026.9995967
CGCAGAG409600.026.3894258
GTGGTAT63250.023.856051
TGGTATC60500.023.5233462
GCAGAGT470250.023.040549
GTACATG319050.021.7522641
TACATGG318300.021.3725662
GAGTACT287450.021.35035112-13
ACATGGG321550.020.503323
CAGAGTA462500.020.49945610-11
GTACTTT318600.019.2901814-15
AGAGTAC432400.019.08285110-11
CATGGGG220850.018.3612084
Produced by FastQC (version 0.11.5)