##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139711_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9802728
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.058514017730573	32.0	28.0	33.0	18.0	33.0
2	30.697103398156106	33.0	31.0	33.0	25.0	33.0
3	31.602373543364664	33.0	32.0	33.0	27.0	34.0
4	30.890987080331108	33.0	31.0	33.0	27.0	34.0
5	31.809039381690486	33.0	32.0	33.0	30.0	34.0
6	35.247627599174436	37.0	35.0	38.0	29.0	38.0
7	35.86165167492151	38.0	36.0	38.0	31.0	38.0
8	36.00845121888519	38.0	37.0	38.0	31.0	38.0
9	36.309514861577306	38.0	37.0	38.0	33.0	38.0
10-11	36.44572163993533	38.0	37.5	38.0	33.5	38.0
12-13	36.48734724660319	38.0	38.0	38.0	34.0	38.0
14-15	36.49451152781144	38.0	38.0	38.0	34.0	38.0
16-17	36.48604505806955	38.0	38.0	38.0	34.0	38.0
18-19	36.501240471019905	38.0	38.0	38.0	34.0	38.0
20-21	36.49682514241367	38.0	38.0	38.0	34.0	38.0
22-23	36.48477413898341	38.0	38.0	38.0	34.0	38.0
24-25	36.47096831663957	38.0	38.0	38.0	34.0	38.0
26-27	36.412968394477275	38.0	38.0	38.0	34.0	38.0
28-29	36.39557700971766	38.0	38.0	38.0	34.0	38.0
30-31	36.396771920781234	38.0	38.0	38.0	34.0	38.0
32-33	36.38079349800017	38.0	38.0	38.0	33.5	38.0
34-35	36.34070031008889	38.0	37.5	38.0	33.0	38.0
36-37	36.31737256293529	38.0	37.0	38.0	33.0	38.0
38-39	36.30309024230101	38.0	37.0	38.0	33.0	38.0
40-41	36.29998214285379	38.0	37.0	38.0	33.0	38.0
42-43	36.24156530263653	38.0	37.0	38.0	33.0	38.0
44-45	36.18628449486211	38.0	37.0	38.0	33.0	38.0
46-47	36.14176237437405	38.0	37.0	38.0	33.0	38.0
48-49	36.08809911702514	38.0	37.0	38.0	33.0	38.0
50-51	36.055636503349604	38.0	37.0	38.0	32.5	38.0
52-53	36.033548660880456	38.0	37.0	38.0	32.0	38.0
54-55	35.98866522330562	38.0	37.0	38.0	31.0	38.0
56-57	35.98921207852139	38.0	37.0	38.0	31.0	38.0
58-59	35.96496247807245	38.0	37.0	38.0	31.0	38.0
60-61	35.94990084279233	38.0	37.0	38.0	31.0	38.0
62-63	35.9452285023119	38.0	37.0	38.0	31.0	38.0
64-65	35.92713813308047	38.0	37.0	38.0	31.0	38.0
66-67	35.93015409007816	38.0	37.0	38.0	31.0	38.0
68-69	35.91369730152284	38.0	37.0	38.0	31.0	38.0
70-71	35.8848152330237	38.0	37.0	38.0	31.0	38.0
72-73	35.85772916250781	38.0	37.0	38.0	31.0	38.0
74-75	35.871160908475076	38.0	37.0	38.0	31.0	38.0
76-77	35.849943648717996	38.0	37.0	38.0	31.0	38.0
78-79	35.836065735999064	38.0	37.0	38.0	31.0	38.0
80-81	35.791537506333086	38.0	37.0	38.0	31.0	38.0
82-83	35.79216134810039	38.0	37.0	38.0	31.0	38.0
84-85	35.785182250139556	38.0	37.0	38.0	31.0	38.0
86-87	35.77662044388336	38.0	37.0	38.0	31.0	38.0
88-89	35.74914586358374	38.0	37.0	38.0	31.0	38.0
90-91	35.7282768528379	38.0	36.5	38.0	31.0	38.0
92-93	35.67561825130609	38.0	36.0	38.0	30.0	38.0
94-95	35.66584333522469	38.0	36.0	38.0	30.0	38.0
96-97	35.69789836488039	38.0	36.0	38.0	31.0	38.0
98-99	35.73549740409395	38.0	36.0	38.0	31.0	38.0
100-101	35.206931674873594	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	15.0
20	110.0
21	804.0
22	3316.0
23	9613.0
24	21573.0
25	39408.0
26	63283.0
27	94665.0
28	131771.0
29	174227.0
30	221652.0
31	276999.0
32	345423.0
33	440750.0
34	590477.0
35	877757.0
36	1706292.0
37	4804590.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.52147402233337	18.217734899917655	14.326267137066335	22.934523940682634
2	14.582277504792543	20.498161328152733	42.643241758824686	22.276319408230037
3	18.212876124751453	26.580568893707614	30.210000531497123	24.99655445004381
4	12.344155626882639	19.290079251408386	38.139077203815106	30.226687917893873
5	14.347097302426452	37.111776185731166	34.7389410874824	13.802185424359987
6	29.588814460627695	38.1135740989651	18.673914036990517	13.623697403416681
7	26.407363338042227	33.26311818506032	22.708219589485704	17.621298887411747
8	24.3545470199724	36.78336275371509	20.8075139899832	18.054576236329314
9	25.54860238904925	17.326982856200846	21.140778362921015	35.98363639182889
10-11	24.074436218162944	26.980045758690846	28.59636623601104	20.349151787135174
12-13	25.126668821168963	24.550732204341486	28.80645061252337	21.516148361966177
14-15	22.305285834718664	25.80469436671098	26.652494081239425	25.237525717330932
16-17	21.584180444464028	29.03958469519913	27.279615429500847	22.096619430835986
18-19	21.60027290362438	27.852721201689977	29.397061715881538	21.149944178804102
20-21	22.447384821361442	26.900721829967555	29.455573695017172	21.196319653653827
22-23	22.209578062907312	26.8110029079866	29.31457705950247	21.664841969603618
24-25	21.943111824562735	27.013949856758668	29.365529062337632	21.677409256340965
26-27	21.823042195188787	27.158475243553504	29.357032012659527	21.66145054859818
28-29	21.782046640743967	27.103208017027846	29.399201284879332	21.715544057348854
30-31	22.03962805826399	27.015445098829925	29.30038849133395	21.644538351572134
32-33	21.761683029688633	27.157047365684665	29.29390077825147	21.787368826375232
34-35	21.944101276714072	27.147990161351355	29.21897238829041	21.688936173644166
36-37	21.92308106861755	27.084483051421298	29.27585152885495	21.716584351106203
38-39	21.772466728244623	27.21282500777333	29.30625929907163	21.708448964910424
40-41	22.032181330471992	27.08903871071689	29.134626318425767	21.74415364038536
42-43	21.966490623351863	27.160332572904988	28.951032528259034	21.92214427548412
44-45	21.958671464224093	27.326888306923557	28.823632531322733	21.890807697529613
46-47	22.14930925102694	27.256024254793676	28.486847282894605	22.107819211284777
48-49	22.020745826182605	27.402356963353085	28.43418921399115	22.142707996473156
50-51	22.03750490697722	27.56390070069647	28.32638056840834	22.07221382391797
52-53	22.232626448390477	27.478002771988773	28.239948467525718	22.04942231209503
54-55	22.158170645678055	27.41278856739274	28.22715000872467	22.201890778204532
56-57	22.145283326794505	27.56195868524846	28.169171593283256	22.12358639467378
58-59	22.079595248375096	27.593167987578443	28.265827744341017	22.061409019705444
60-61	22.195149947789997	27.581505680428	28.21180083470155	22.011543537080456
62-63	22.119908944701244	27.674497462059144	28.1870843294463	22.018509263793312
64-65	22.11631042843596	27.638935277960652	28.15116496459603	22.093589329007354
66-67	22.173362051243302	27.649996216111017	28.02270053532992	22.15394119731576
68-69	22.073056572037864	27.614193407423095	28.154498374691656	22.158251645847386
70-71	22.14179506277564	27.35680807786939	28.216564922325478	22.28483193702949
72-73	22.19530478074965	27.410699194955985	28.10961937137048	22.28437665292388
74-75	22.05281184600715	27.551523618952857	28.109785693698917	22.285878841341077
76-77	22.227955692834573	27.43808633482666	28.03320505169719	22.300752920641578
78-79	22.170845946202554	27.499101987674983	27.965157369687816	22.36489469643465
80-81	22.209441520152133	27.470598449551005	27.981110768477453	22.338849261819416
82-83	22.257943979242082	27.47927119673913	28.014592742927608	22.248192081091183
84-85	22.289176537930068	27.448423924503956	27.981864171791372	22.280535365774604
86-87	22.156735064189146	27.515379371208425	28.0376581671388	22.290227397463628
88-89	22.22724697369216	27.53051864827909	27.934476337964043	22.307758040064705
90-91	22.267870943774234	27.540196562967484	27.93343527452808	22.258497218730202
92-93	22.321079159354067	27.557033912077618	27.860546976535698	22.261339952032614
94-95	22.200837436204587	27.581501824398064	27.900055294687476	22.31760544470987
96-97	22.2521854048216	27.573711995297334	27.921924099372113	22.25217850050895
98-99	22.440275632366628	28.176160738621412	27.966708009903527	21.41685561910843
100-101	23.078389213366776	30.6486356678293	25.33545002043831	20.937525098365615
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2766.0
1	2538.0
2	3147.0
3	4132.5
4	4906.0
5	7241.0
6	9889.5
7	13074.5
8	16794.0
9	18442.0
10	17322.5
11	15508.0
12	15800.0
13	18973.5
14	26559.0
15	36501.0
16	44917.0
17	50989.0
18	52825.5
19	50118.0
20	45648.5
21	41438.0
22	39864.5
23	43217.5
24	51593.5
25	65167.5
26	83380.5
27	103369.0
28	123966.5
29	144819.0
30	163921.5
31	183165.5
32	204434.0
33	227229.0
34	249159.5
35	269149.0
36	292568.0
37	313412.0
38	328953.0
39	342602.5
40	356328.0
41	369448.5
42	377558.5
43	386004.5
44	395963.5
45	405153.0
46	408713.5
47	406585.5
48	404600.0
49	400676.0
50	393791.5
51	381752.5
52	365836.0
53	348595.5
54	328860.5
55	306831.0
56	281903.0
57	255747.5
58	229498.5
59	200217.0
60	167958.5
61	137075.0
62	108722.0
63	84135.0
64	64073.5
65	47913.0
66	35414.5
67	27049.5
68	20892.0
69	15468.0
70	11378.5
71	8348.0
72	6026.0
73	4143.5
74	2726.0
75	1793.5
76	1194.5
77	721.5
78	447.5
79	282.5
80	172.5
81	110.0
82	79.5
83	52.0
84	30.5
85	20.0
86	14.0
87	9.0
88	3.5
89	3.5
90	3.5
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0023360844042597125
4	0.0
5	0.050975605974173714
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	9.491492764828978E-4
28-29	2.552679780259198E-4
30-31	0.0
32-33	0.0
34-35	1.1299569090932717E-4
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	1.4980262701376864E-4
48-49	0.0
50-51	5.445389873034201E-6
52-53	1.6463345267246466E-5
54-55	9.62331417095508E-4
56-57	0.001874044362868979
58-59	0.00822026483769056
60-61	0.007555318583830176
62-63	0.0036243532490494662
64-65	0.004828144896786563
66-67	0.006249731092493806
68-69	0.003315583868430094
70-71	0.0050291311678945985
72-73	8.958816022114928E-4
74-75	0.001396136489702522
76-77	0.0012446435874184315
78-79	9.8538683715305E-4
80-81	3.759847746218799E-5
82-83	0.0
84-85	0.0
86-87	2.9145298575125215E-4
88-89	8.387864280686247E-4
90-91	0.0
92-93	0.0
94-95	1.224837749826073E-4
96-97	2.830760172336679E-4
98-99	1.4277620914316E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	177.0
20-21	613.0
22-23	1381.0
24-25	1656.0
26-27	3603.0
28-29	8003.0
30-31	19412.0
32-33	25221.0
34-35	34852.0
36-37	53479.0
38-39	62013.0
40-41	62699.0
42-43	73216.0
44-45	88265.0
46-47	87615.0
48-49	79992.0
50-51	72392.0
52-53	69244.0
54-55	74808.0
56-57	77953.0
58-59	78203.0
60-61	76815.0
62-63	74325.0
64-65	79699.0
66-67	85273.0
68-69	87095.0
70-71	89127.0
72-73	78813.0
74-75	80683.0
76-77	86124.0
78-79	88546.0
80-81	89124.0
82-83	85518.0
84-85	84968.0
86-87	88791.0
88-89	92285.0
90-91	95299.0
92-93	93443.0
94-95	99933.0
96-97	156057.0
98-99	811918.0
100-101	6304095.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.64814316880582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.42313948442363	77.44573996334813
2	6.63654714966488	11.368158808420555
3	1.628308582113664	4.183848196915989
4	0.5888442281389328	2.0173365902307303
5	0.2642470231464116	1.13161334351873
6	0.1480972003187643	0.7610550129480504
7	0.09038201039903437	0.5418735956578694
8	0.056540820390618925	0.3874093023757971
9	0.03442572102016403	0.2653649174361939
>10	0.1269908405459491	1.6779300015816425
>50	0.0018994167967148396	0.11048611652714199
>100	5.386707764304834E-4	0.07464341361488262
>500	1.94261412941115E-5	0.013693180420282466
>1k	1.942612357228683E-5	0.020847557004064723
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.0201241939998744E-5	0.0
3	0.0	0.0	0.0	3.0603725819996226E-5	0.0
4	0.0	1.0201241939998744E-5	0.0	5.100620969999372E-5	2.0402483879997488E-5
5	0.0	1.0201241939998744E-5	0.0	5.100620969999372E-5	2.0402483879997488E-5
6	8.160993551998995E-5	1.0201241939998744E-5	0.0	5.100620969999372E-5	2.0402483879997488E-5
7	8.160993551998995E-5	1.0201241939998744E-5	0.0	5.100620969999372E-5	2.0402483879997488E-5
8	8.160993551998995E-5	2.0402483879997488E-5	0.0	5.100620969999372E-5	2.0402483879997488E-5
9	8.160993551998995E-5	1.3261614521998367E-4	0.0	5.100620969999372E-5	5.100620969999372E-5
10-11	1.1221366133998617E-4	1.3261614521998367E-4	0.0	6.120745163999245E-5	5.100620969999372E-5
12-13	1.3261614521998367E-4	1.3771676618998304E-4	0.0	6.120745163999245E-5	5.100620969999372E-5
14-15	1.428173871599824E-4	1.78521733949978E-4	0.0	6.630807260999183E-5	8.160993551998994E-5
16-17	1.8362235491997737E-4	1.8362235491997737E-4	0.0	7.14086935799912E-5	1.0201241939998743E-4
18-19	1.8362235491997737E-4	1.9892421782997547E-4	0.0	7.14086935799912E-5	1.224149032799849E-4
20-21	1.8362235491997737E-4	2.2442732267997234E-4	0.0	8.160993551998995E-5	1.224149032799849E-4
22-23	1.8872297588997674E-4	2.6013166946996794E-4	0.0	8.671055648998932E-5	1.2751552424998427E-4
24-25	1.989242178299755E-4	3.3154036304995914E-4	0.0	1.0201241939998743E-4	1.3771676618998304E-4
26-27	2.0402483879997487E-4	3.57043467899956E-4	0.0	1.6832049200997927E-4	1.428173871599824E-4
28-29	2.0402483879997487E-4	3.57043467899956E-4	0.0	4.0804967759994974E-4	1.428173871599824E-4
30-31	2.142260807399736E-4	4.0804967759994974E-4	0.0	0.0011374384763098598	1.428173871599824E-4
32-33	2.142260807399736E-4	4.0804967759994974E-4	0.0	0.002647222283429674	1.5301862909998114E-4
34-35	2.142260807399736E-4	4.947602340899391E-4	0.0	0.004855791163439402	1.7342111297997864E-4
36-37	2.448298065599698E-4	5.865714115499277E-4	0.0	0.008150792310058996	1.7342111297997864E-4
38-39	2.448298065599698E-4	6.018732744599259E-4	0.0	0.013174903965508376	1.78521733949978E-4
40-41	2.550310484999686E-4	6.630807260999183E-4	0.0	0.018688675234077697	1.8362235491997737E-4
42-43	2.703329114099667E-4	6.987850728899139E-4	0.0	0.023881107381537058	1.8872297588997674E-4
44-45	2.7543353237996607E-4	7.599925245299064E-4	0.0	0.029884538263226318	1.9382359685997613E-4
46-47	2.7543353237996607E-4	8.058981132599007E-4	0.0	0.036438836209675515	1.9382359685997613E-4
48-49	2.7543353237996607E-4	8.109987342299E-4	0.0	0.04272790186568474	1.9382359685997613E-4
50-51	3.1623850013996107E-4	9.793192262398795E-4	0.0	0.049083275594303956	1.9382359685997613E-4
52-53	3.417416049899579E-4	9.895204681798781E-4	0.0	0.05527032883091319	2.0912545976997424E-4
54-55	3.57043467899956E-4	0.0010252248149698736	0.0	0.061737916220872394	2.2442732267997234E-4
56-57	3.7234533080995414E-4	0.0010405266778798718	0.0	0.06854724521582156	2.2442732267997234E-4
58-59	3.8254657274995287E-4	0.0010762310246698673	0.0	0.07485671335571079	2.2442732267997234E-4
60-61	4.0804967759994974E-4	0.001111935371459863	0.0	0.08132940136663999	2.2442732267997234E-4
62-63	4.641565082699428E-4	0.001152740339219858	0.0	0.08756746081294921	2.448298065599698E-4
64-65	4.692571292399422E-4	0.001387368903839829	0.0	0.09353518734784848	2.448298065599698E-4
66-67	4.7945837117994094E-4	0.0014128720086898261	0.0	0.0997936492780377	2.448298065599698E-4
68-69	4.7945837117994094E-4	0.0014536769764498208	0.0	0.10590929382106695	2.448298065599698E-4
70-71	4.7945837117994094E-4	0.0014638782183898197	0.0	0.11165769365425623	2.703329114099667E-4
72-73	4.7945837117994094E-4	0.0014740794603298184	0.0	0.11607483141427569	3.060372581999623E-4
74-75	4.7945837117994094E-4	0.0015199850490598128	0.0	0.11965546733521526	3.060372581999623E-4
76-77	4.845589921499403E-4	0.0015811925006998053	0.0	0.12264953184460489	3.1623850013996107E-4
78-79	4.896596131199396E-4	0.0016219974684598003	0.0	0.1257252062895145	3.264397420799598E-4
80-81	4.896596131199396E-4	0.0016219974684598003	0.0	0.12711767581432434	3.264397420799598E-4
82-83	4.896596131199396E-4	0.001632198710399799	0.0	0.12761753666938427	3.57043467899956E-4
84-85	4.998608550599384E-4	0.0016423999523397976	0.0	0.1282041080809342	3.57043467899956E-4
86-87	4.998608550599384E-4	0.0016423999523397976	0.0	0.12842343478264417	3.57043467899956E-4
88-89	4.998608550599384E-4	0.001673003678159794	0.0	0.12845403850846418	3.6724470983995474E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14795	0.0	54.84689	1
GTATCAA	28055	0.0	38.99301	1
TCAACGC	33700	0.0	31.926504	4
ATCAACG	33940	0.0	31.726646	3
TATCAAC	34785	0.0	31.165438	2
CAACGCA	34715	0.0	31.01773	5
AACGCAG	35630	0.0	30.264957	6
ACGCAGA	39955	0.0	26.999596	7
CGCAGAG	40960	0.0	26.389425	8
GTGGTAT	6325	0.0	23.85605	1
TGGTATC	6050	0.0	23.523346	2
GCAGAGT	47025	0.0	23.04054	9
GTACATG	31905	0.0	21.752264	1
TACATGG	31830	0.0	21.372566	2
GAGTACT	28745	0.0	21.350351	12-13
ACATGGG	32155	0.0	20.50332	3
CAGAGTA	46250	0.0	20.499456	10-11
GTACTTT	31860	0.0	19.29018	14-15
AGAGTAC	43240	0.0	19.082851	10-11
CATGGGG	22085	0.0	18.361208	4
>>END_MODULE