FastQCFastQC Report
Fri 27 May 2016
SRR2139710_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139710_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12147735
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA211950.054.717451
GTATCAA413750.038.3989031
CTTATAC109400.032.6476361
TCAACGC496550.031.6055584
ATCAACG495950.031.6024743
TATCAAC509850.030.9222132
CAACGCA507500.030.8905075
AACGCAG520850.030.1466486
TGGTATC85450.027.9309982
GTGGTAT92750.027.6807231
ACGCAGA581650.026.995417
CGCAGAG593150.026.4861878
TATACAC171650.024.8713913
GCAGAGT673850.023.3204449
TTATACA176350.021.5398732
GAGTACT393600.021.48289512-13
ACATCTC174200.020.9251128
ACACATC176200.020.83066
GTACATG487100.020.5999581
TACATGG478200.020.3770522
Produced by FastQC (version 0.11.5)