##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139710_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12147735
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.977783018809678	32.0	27.0	33.0	18.0	33.0
2	30.695922737860187	33.0	31.0	33.0	25.0	33.0
3	31.612780489531588	33.0	32.0	33.0	27.0	34.0
4	30.8989279071366	33.0	31.0	33.0	27.0	34.0
5	31.82878709487818	33.0	32.0	33.0	30.0	34.0
6	35.27368879877607	37.0	35.0	38.0	29.0	38.0
7	35.915336809701564	38.0	36.0	38.0	31.0	38.0
8	36.0378156915672	38.0	37.0	38.0	31.0	38.0
9	36.33270556198337	38.0	37.0	38.0	33.0	38.0
10-11	36.46294560261646	38.0	37.5	38.0	34.0	38.0
12-13	36.50086505838331	38.0	38.0	38.0	34.0	38.0
14-15	36.509809729962	38.0	38.0	38.0	34.0	38.0
16-17	36.50252837257316	38.0	38.0	38.0	34.0	38.0
18-19	36.52331446973449	38.0	38.0	38.0	34.0	38.0
20-21	36.518781738811924	38.0	38.0	38.0	34.0	38.0
22-23	36.5061845983765	38.0	38.0	38.0	34.0	38.0
24-25	36.488734310607846	38.0	38.0	38.0	34.0	38.0
26-27	36.43282191884222	38.0	38.0	38.0	34.0	38.0
28-29	36.41143876101116	38.0	38.0	38.0	34.0	38.0
30-31	36.41724075368754	38.0	38.0	38.0	34.0	38.0
32-33	36.39452152716981	38.0	38.0	38.0	34.0	38.0
34-35	36.345540834215726	38.0	38.0	38.0	33.0	38.0
36-37	36.32166624458028	38.0	37.5	38.0	33.0	38.0
38-39	36.287739784384065	38.0	37.0	38.0	33.0	38.0
40-41	36.304666646643156	38.0	37.0	38.0	33.0	38.0
42-43	36.252414536429285	38.0	37.0	38.0	33.0	38.0
44-45	36.194861214520614	38.0	37.0	38.0	33.0	38.0
46-47	36.15334613588165	38.0	37.0	38.0	33.0	38.0
48-49	36.105445915244324	38.0	37.0	38.0	33.0	38.0
50-51	36.076354659044334	38.0	37.0	38.0	33.0	38.0
52-53	36.065596717040165	38.0	37.0	38.0	32.5	38.0
54-55	36.02132571462051	38.0	37.0	38.0	31.0	38.0
56-57	36.01980520499497	38.0	37.0	38.0	31.0	38.0
58-59	35.9928701957831	38.0	37.0	38.0	31.0	38.0
60-61	35.97770332281928	38.0	37.0	38.0	31.0	38.0
62-63	35.97117388412762	38.0	37.0	38.0	31.0	38.0
64-65	35.95823113650327	38.0	37.0	38.0	31.0	38.0
66-67	35.95818316619825	38.0	37.0	38.0	31.0	38.0
68-69	35.93916951016364	38.0	37.0	38.0	31.0	38.0
70-71	35.9107255482197	38.0	37.0	38.0	31.0	38.0
72-73	35.88379495815541	38.0	37.0	38.0	31.0	38.0
74-75	35.901617011638805	38.0	37.0	38.0	31.0	38.0
76-77	35.88533902463104	38.0	37.0	38.0	31.0	38.0
78-79	35.87388465217588	38.0	37.0	38.0	31.0	38.0
80-81	35.83316011369981	38.0	37.0	38.0	31.0	38.0
82-83	35.832734282210275	38.0	37.0	38.0	31.0	38.0
84-85	35.83089347600094	38.0	37.0	38.0	31.0	38.0
86-87	35.824521421595826	38.0	37.0	38.0	31.0	38.0
88-89	35.79651522293335	38.0	37.0	38.0	31.0	38.0
90-91	35.77737385933259	38.0	37.0	38.0	31.0	38.0
92-93	35.727183859189935	38.0	37.0	38.0	31.0	38.0
94-95	35.7173772579975	38.0	36.0	38.0	31.0	38.0
96-97	35.743343207286074	38.0	36.0	38.0	31.0	38.0
98-99	35.773846414643884	38.0	36.5	38.0	31.0	38.0
100-101	35.23879856426545	38.0	35.5	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	9.0
19	28.0
20	200.0
21	1014.0
22	4183.0
23	12014.0
24	26848.0
25	48749.0
26	78800.0
27	116877.0
28	162139.0
29	213542.0
30	270132.0
31	337884.0
32	421475.0
33	538818.0
34	725360.0
35	1082014.0
36	2117176.0
37	5990470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.70110971304527	19.249769607256003	14.750412319662884	22.298708360035842
2	13.864732808214864	21.01879074576454	44.062963177909296	21.0535132681113
3	17.476724729371007	28.06422948963247	31.054949675824755	23.404096105171774
4	11.671723164853367	20.24027524472669	39.330294906828314	28.757706683591632
5	13.213107849190864	38.16338795829418	35.563219066741155	13.060285125773802
6	27.66255602381843	39.59893757972165	19.436248815108332	13.302257581351585
7	24.98912760280003	34.290285390651015	23.584445989314055	17.1361410172349
8	23.357366620197098	37.71382072460422	21.546773945924897	17.382038709273786
9	25.10120610961632	17.683238891859265	21.88596474980727	35.329590248717146
10-11	23.155353652347536	27.877995362921563	29.293357979903252	19.67329300482765
12-13	24.374321632798214	25.40800404355215	29.56921598964745	20.648458334002182
14-15	21.569621826620352	26.762631058382492	27.48281058156109	24.18493653343607
16-17	20.555791699267054	30.11529845338651	28.132315535872145	21.196594311474293
18-19	20.582787655476515	28.752442327726115	30.440929934675065	20.223840082122305
20-21	21.468496260267543	27.707590877316377	30.47197795449857	20.35193490791751
22-23	21.226136421528146	27.77842871584955	30.326939812728344	20.668495049893956
24-25	20.973195641513065	27.95385058781965	30.418163185536923	20.654790585130364
26-27	20.803646702362265	28.054203687938728	30.455597531439434	20.68655207825958
28-29	20.529322775932812	28.013898890176357	30.619346941371113	20.83743139251972
30-31	20.987355026694583	27.821031029304223	30.332188413352522	20.859425530648668
32-33	20.890973070321248	27.958926324057547	30.420829411001137	20.72927119462007
34-35	21.00757941774261	28.239744802629485	30.32003080077145	20.43264497885646
36-37	20.80346344085465	28.08869619349128	30.268020414082663	20.83981995157141
38-39	20.742285670687554	28.103869549746026	30.35611678409294	20.797727995473476
40-41	20.94875364744419	27.95362588807751	30.297543831820974	20.800076632657323
42-43	21.044051369190495	28.024203622855833	30.024678032860706	20.90706697509296
44-45	20.900579516705456	28.333398304796773	29.832350338090226	20.93367184040755
46-47	21.169520033681604	28.21543144785892	29.36732846224441	21.24772005621507
48-49	21.121192464183558	28.33481860153093	29.275365550718362	21.268623383567146
50-51	21.007466821174106	28.477389714308092	29.27112272883633	21.244020735681474
52-53	21.19781407828603	28.486516892951425	29.149176603994988	21.166492424767565
54-55	21.117903962672102	28.575786122141555	28.93999829878312	21.36631161640322
56-57	21.30031438080358	28.704766043808906	28.66786128894891	21.327058286438604
58-59	21.03083828765432	28.92567329883702	28.969075982083027	21.07441243142564
60-61	21.271470342425186	29.13074522601654	28.63910628126763	20.95867815029064
62-63	21.053876641017382	29.263748475318568	28.627798299715657	21.05457658394839
64-65	21.00941575139569	29.165061224957796	28.650963157531393	21.17455986611512
66-67	21.14193498912855	29.16356588207092	28.50804137476286	21.186457754037672
68-69	21.084563738687223	28.999817747927036	28.687146580816563	21.228471932569178
70-71	21.178649700984614	28.63451681288177	28.81865061525768	21.368182870875934
72-73	21.222159334060382	28.651728288001564	28.779469622717045	21.34664275522101
74-75	21.161446285678185	28.727072428203428	28.713757291546944	21.397723994571443
76-77	21.241910765215028	28.616821420815103	28.698817102724817	21.442450711245055
78-79	21.19991594247731	28.64670920304182	28.68480620804071	21.46856864644016
80-81	21.272319575921877	28.694107502567018	28.59156886793455	21.442004053576554
82-83	21.305744788758858	28.638840783868076	28.724242064961857	21.33117236241121
84-85	21.3780723972354	28.663317026056873	28.59962523395926	21.35898534274846
86-87	21.163992988722395	28.7991348407311	28.628299603681455	21.408572566865047
88-89	21.31718503932112	28.693395836678143	28.574107444111892	21.415311679888845
90-91	21.358812508225476	28.68075661885745	28.60291819300781	21.357512679909266
92-93	21.35036717231768	28.720323649684254	28.517977245164545	21.41133193283352
94-95	21.241578484073536	28.783450783975688	28.544546045842463	21.430424686108314
96-97	21.267464450816316	28.827649365088654	28.542313769091226	21.362572415003804
98-99	21.38236000763704	29.38681551238244	28.664343713268053	20.566480766712466
100-101	24.960669245086446	36.24702589561288	29.752538105610622	9.03976675369006
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4861.0
1	4162.0
2	3902.5
3	4991.5
4	6931.0
5	10586.0
6	13616.5
7	14499.5
8	14025.5
9	13049.5
10	12532.5
11	13258.5
12	15851.0
13	23839.5
14	38571.0
15	57226.5
16	74578.0
17	85821.5
18	90848.5
19	86423.0
20	78580.0
21	72831.0
22	70430.5
23	76469.0
24	90095.5
25	111397.5
26	139770.0
27	170911.0
28	202317.0
29	234763.5
30	266868.0
31	297684.0
32	329351.5
33	361549.0
34	391541.5
35	420548.0
36	450895.0
37	474670.0
38	489521.0
39	498461.0
40	506514.5
41	512038.5
42	510627.0
43	510755.5
44	514229.5
45	525212.5
46	524265.5
47	506651.0
48	490771.0
49	468430.5
50	441397.5
51	414613.0
52	385262.0
53	354011.0
54	323121.5
55	292354.5
56	261185.5
57	230230.5
58	201581.0
59	173610.0
60	145643.0
61	118822.0
62	92868.0
63	70980.5
64	54492.0
65	41002.5
66	29573.0
67	21889.5
68	16695.0
69	12450.0
70	9344.0
71	6938.0
72	5147.0
73	3860.5
74	2721.5
75	1951.0
76	1338.0
77	847.5
78	609.0
79	417.0
80	258.5
81	165.5
82	126.5
83	93.0
84	70.5
85	55.0
86	40.0
87	29.5
88	18.5
89	11.5
90	8.5
91	4.5
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0024119722730204436
4	0.0
5	0.05117003293206512
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	4.115993639966628E-6
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.819212530864669E-4
28-29	2.268198187074553E-4
30-31	0.0
32-33	0.0
34-35	7.893792429653637E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	1.1772501381415834E-4
48-49	0.0
50-51	8.900197281772949E-6
52-53	3.1528833117886304E-5
54-55	9.996366775057574E-4
56-57	0.002238547821000762
58-59	0.008697389596902635
60-61	0.007720182387319163
62-63	0.004114360105159261
64-65	0.005126003177453153
66-67	0.006619508386989602
68-69	0.0036791548042056066
70-71	0.005527972432918674
72-73	9.2867243514478E-4
74-75	0.001674512773868467
76-77	0.001365036529095959
78-79	0.0010387623520529735
80-81	6.311684221389057E-5
82-83	0.0
84-85	0.0
86-87	2.6189102561654333E-4
88-89	9.284777557454673E-4
90-91	0.0
92-93	5.682770045800854E-6
94-95	1.9018288620280217E-4
96-97	3.74508516089588E-4
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1541.0
20-21	4801.0
22-23	3456.0
24-25	3973.0
26-27	6905.0
28-29	13295.0
30-31	29468.0
32-33	38132.0
34-35	50762.0
36-37	77503.0
38-39	93269.0
40-41	92430.0
42-43	106902.0
44-45	125704.0
46-47	125163.0
48-49	113034.0
50-51	137216.0
52-53	95159.0
54-55	102049.0
56-57	108749.0
58-59	110465.0
60-61	108581.0
62-63	105413.0
64-65	116395.0
66-67	126872.0
68-69	126196.0
70-71	136772.0
72-73	107244.0
74-75	108703.0
76-77	115032.0
78-79	119605.0
80-81	120908.0
82-83	112462.0
84-85	111291.0
86-87	115375.0
88-89	120944.0
90-91	127475.0
92-93	121342.0
94-95	126604.0
96-97	185152.0
98-99	936907.0
100-101	7458486.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.8104020482741
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.13291135751234	74.70265136606285
2	7.416597185103846	12.431759838273026
3	1.9167330504484936	4.8192650273190925
4	0.7044604944682301	2.3616446907403335
5	0.3124533738674041	1.309342144258342
6	0.1646737002613555	0.828082141540871
7	0.09995460058389594	0.5864064683057679
8	0.06489744729329605	0.43512649196462555
9	0.042633401202933345	0.3215810245952895
>10	0.14025861621803073	1.8005495170744412
>50	0.003156848936611437	0.1777549092945722
>100	0.0011845205946501706	0.1697838361067283
>500	6.83286290326514E-5	0.034872966745442546
>1k	1.707487989055463E-5	0.021179577718589936
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	8.231987279933255E-6	0.0	0.0	0.0
4	0.0	4.115993639966627E-5	0.0	0.0	8.231987279933255E-6
5	8.231987279933255E-6	4.115993639966627E-5	0.0	0.0	8.231987279933255E-6
6	8.231987279933255E-6	4.115993639966627E-5	0.0	0.0	8.231987279933255E-6
7	8.231987279933255E-6	4.115993639966627E-5	0.0	0.0	8.231987279933255E-6
8	8.231987279933255E-6	4.115993639966627E-5	0.0	0.0	8.231987279933255E-6
9	8.231987279933255E-6	1.0701583463913232E-4	0.0	0.0	8.231987279933255E-6
10-11	1.646397455986651E-5	1.0701583463913232E-4	0.0	0.0	8.231987279933255E-6
12-13	2.4695961839799766E-5	1.0701583463913232E-4	0.0	0.0	1.646397455986651E-5
14-15	4.115993639966627E-5	1.3994378375886533E-4	0.0	0.0	4.115993639966627E-5
16-17	6.173990459949942E-5	1.5229176467876522E-4	0.0	0.0	4.115993639966627E-5
18-19	6.997189187943266E-5	1.6052375195869847E-4	0.0	0.0	4.115993639966627E-5
20-21	7.40878855193993E-5	1.646397455986651E-4	0.0	0.0	5.762391095953278E-5
22-23	7.40878855193993E-5	1.811037201585316E-4	0.0	4.115993639966628E-6	6.585589823946604E-5
24-25	9.05518600792658E-5	2.222636565581979E-4	0.0	1.646397455986651E-5	7.40878855193993E-5
26-27	9.05518600792658E-5	2.3049564383813113E-4	0.0	7.820387915936592E-5	7.40878855193993E-5
28-29	9.05518600792658E-5	2.387276311180644E-4	0.0	3.0458352935753045E-4	7.820387915936592E-5
30-31	1.0701583463913232E-4	2.716555802377974E-4	0.0	0.0012471460729098882	8.643586643929918E-5
32-33	1.2759580283896546E-4	2.716555802377974E-4	0.0	0.002914123497096372	9.878384735919906E-5
34-35	1.4405977739883198E-4	3.2927949119733024E-4	0.0	0.005375487693796416	9.878384735919906E-5
36-37	1.5640775831873185E-4	3.86903402156863E-4	0.0	0.008754718472209017	9.878384735919906E-5
38-39	1.646397455986651E-4	3.992513830767629E-4	0.0	0.013982030394966634	1.0701583463913232E-4
40-41	1.646397455986651E-4	4.280633385565293E-4	0.0	0.01979381341459951	1.0701583463913232E-4
42-43	1.646397455986651E-4	4.445273131163958E-4	0.0	0.025268084955755128	1.1113182827909894E-4
44-45	1.7287173287859836E-4	5.144992049958284E-4	0.0	0.03132271160014603	1.1524782191906557E-4
46-47	1.811037201585316E-4	5.597751350354613E-4	0.0	0.038402220660888636	1.1524782191906557E-4
48-49	1.811037201585316E-4	5.638911286754279E-4	0.0	0.045638137479949964	1.1524782191906557E-4
50-51	1.811037201585316E-4	6.585589823946605E-4	0.0	0.05297283814637049	1.193638155590322E-4
52-53	1.811037201585316E-4	6.667909696745937E-4	0.0	0.05985477951239469	1.2347980919899884E-4
54-55	1.811037201585316E-4	6.667909696745937E-4	0.0	0.06684785270669799	1.2347980919899884E-4
56-57	1.811037201585316E-4	6.832549442344602E-4	0.0	0.07440070103603677	1.3994378375886533E-4
58-59	1.811037201585316E-4	7.038349124342932E-4	0.0	0.08180948958797668	1.481757710387986E-4
60-61	1.811037201585316E-4	7.202988869941598E-4	0.0	0.08925532208267631	1.481757710387986E-4
62-63	1.811037201585316E-4	7.28530874274093E-4	0.0	0.09600555165222159	1.5640775831873185E-4
64-65	1.811037201585316E-4	7.73806804313726E-4	0.0	0.10261172144436803	1.5640775831873185E-4
66-67	1.811037201585316E-4	7.779227979536926E-4	0.0	0.10939076296939307	1.5640775831873185E-4
68-69	1.811037201585316E-4	8.026187597934924E-4	0.0	0.11622742840537763	1.687557392386317E-4
70-71	1.811037201585316E-4	8.149667407133922E-4	0.0	0.12188691966033174	2.057996819983314E-4
72-73	1.811037201585316E-4	8.273147216332922E-4	0.0	0.12642274465157496	2.222636565581979E-4
74-75	1.811037201585316E-4	8.602426707530252E-4	0.0	0.13022180678126416	2.222636565581979E-4
76-77	1.811037201585316E-4	9.096345944326247E-4	0.0	0.13386446115263462	2.263796501981645E-4
78-79	1.811037201585316E-4	9.219825753525245E-4	0.0	0.13698438433172933	2.3049564383813113E-4
80-81	1.811037201585316E-4	9.219825753525245E-4	0.0	0.1383179662710785	2.469596183979977E-4
82-83	1.8521971379849823E-4	9.260985689924911E-4	0.0	0.13894359730435346	2.6753958659783076E-4
84-85	1.934517010784315E-4	9.384465499123911E-4	0.0	0.1395280684012287	2.716555802377974E-4
86-87	1.9756769471839813E-4	9.466785371923243E-4	0.0	0.13975856404506684	2.716555802377974E-4
88-89	1.9756769471839813E-4	9.796064863120573E-4	0.0	0.13986969587334594	2.7988756751773066E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21195	0.0	54.71745	1
GTATCAA	41375	0.0	38.398903	1
CTTATAC	10940	0.0	32.647636	1
TCAACGC	49655	0.0	31.605558	4
ATCAACG	49595	0.0	31.602474	3
TATCAAC	50985	0.0	30.922213	2
CAACGCA	50750	0.0	30.890507	5
AACGCAG	52085	0.0	30.146648	6
TGGTATC	8545	0.0	27.930998	2
GTGGTAT	9275	0.0	27.680723	1
ACGCAGA	58165	0.0	26.99541	7
CGCAGAG	59315	0.0	26.486187	8
TATACAC	17165	0.0	24.871391	3
GCAGAGT	67385	0.0	23.320444	9
TTATACA	17635	0.0	21.539873	2
GAGTACT	39360	0.0	21.482895	12-13
ACATCTC	17420	0.0	20.925112	8
ACACATC	17620	0.0	20.8306	6
GTACATG	48710	0.0	20.599958	1
TACATGG	47820	0.0	20.377052	2
>>END_MODULE