FastQCFastQC Report
Fri 27 May 2016
SRR2139709_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139709_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8409729
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139550.050.7852441
GTATCAA262400.037.883161
CTTATAC85500.035.3544461
TCAACGC312400.031.1684974
ATCAACG312450.031.1373353
CAACGCA319100.030.4609955
TATCAAC327400.030.0517232
AACGCAG329100.029.5844216
TATACAC121950.028.050563
TGGTATC63350.026.4643062
ACGCAGA371450.026.188647
GTGGTAT68900.026.1144941
CGCAGAG375250.026.0249238
TTATACA128150.025.206812
GCAGAGT430150.022.683699
ACATCTC142350.021.9070728
ACACATC142050.021.7448276
GAGTACT252950.021.26943812-13
TACACAT162000.020.4880245
GTACATG297000.019.984971
Produced by FastQC (version 0.11.5)