FastQCFastQC Report
Fri 27 May 2016
SRR2139708_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139708_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9646717
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142600.051.897331
GTATCAA263500.038.552711
CTTATAC94250.033.216731
TCAACGC311900.031.9378854
ATCAACG312900.031.8364753
CAACGCA318000.031.3252415
TATCAAC321600.031.18832
AACGCAG328200.030.3986536
ACGCAGA365700.027.2580747
TATACAC130850.027.0023213
CGCAGAG372350.026.8172388
TTATACA140700.023.8030972
TGGTATC61950.023.4357722
GCAGAGT434900.022.9700589
GTGGTAT66700.022.7941471
ACATCTC147150.021.9893388
GAGTACT278850.021.28905712-13
ACACATC150300.020.9019956
CAGAGTA423050.020.56820110-11
TACACAT172200.019.8463925
Produced by FastQC (version 0.11.5)