##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139708_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9646717
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.241021997431872	32.0	28.0	33.0	18.0	33.0
2	30.792175099570144	33.0	31.0	33.0	25.0	34.0
3	31.641980478954654	33.0	32.0	33.0	27.0	34.0
4	30.975617300683744	33.0	31.0	33.0	27.0	34.0
5	31.848236659166016	33.0	32.0	33.0	30.0	34.0
6	35.28042783881812	37.0	35.0	38.0	29.0	38.0
7	35.86296384562748	38.0	36.0	38.0	31.0	38.0
8	36.02136198252732	38.0	37.0	38.0	31.0	38.0
9	36.306337482482384	38.0	37.0	38.0	33.0	38.0
10-11	36.444086470039494	38.0	37.5	38.0	33.5	38.0
12-13	36.48006705286369	38.0	38.0	38.0	34.0	38.0
14-15	36.49005962339312	38.0	38.0	38.0	34.0	38.0
16-17	36.4792134463984	38.0	38.0	38.0	34.0	38.0
18-19	36.4974635412234	38.0	38.0	38.0	34.0	38.0
20-21	36.49161440187322	38.0	38.0	38.0	34.0	38.0
22-23	36.483481874767904	38.0	38.0	38.0	34.0	38.0
24-25	36.46702747560117	38.0	38.0	38.0	34.0	38.0
26-27	36.40888356578889	38.0	38.0	38.0	34.0	38.0
28-29	36.38502454890299	38.0	38.0	38.0	33.5	38.0
30-31	36.38683326031284	38.0	38.0	38.0	33.5	38.0
32-33	36.36719494474569	38.0	38.0	38.0	33.0	38.0
34-35	36.32118660108601	38.0	37.5	38.0	33.0	38.0
36-37	36.29138390777121	38.0	37.0	38.0	33.0	38.0
38-39	36.256989178698205	38.0	37.0	38.0	33.0	38.0
40-41	36.27927696975445	38.0	37.0	38.0	33.0	38.0
42-43	36.234263362574104	38.0	37.0	38.0	33.0	38.0
44-45	36.178538866212776	38.0	37.0	38.0	33.0	38.0
46-47	36.133732915650384	38.0	37.0	38.0	33.0	38.0
48-49	36.08324943928373	38.0	37.0	38.0	32.5	38.0
50-51	36.05281367048836	38.0	37.0	38.0	32.0	38.0
52-53	36.03009296592822	38.0	37.0	38.0	32.0	38.0
54-55	35.985484491267556	38.0	37.0	38.0	31.0	38.0
56-57	35.983821916670806	38.0	37.0	38.0	31.0	38.0
58-59	35.95710822114734	38.0	37.0	38.0	31.0	38.0
60-61	35.94125983519473	38.0	37.0	38.0	31.0	38.0
62-63	35.936347433662604	38.0	37.0	38.0	31.0	38.0
64-65	35.92141716077521	38.0	37.0	38.0	31.0	38.0
66-67	35.92164591815258	38.0	37.0	38.0	31.0	38.0
68-69	35.90186876047351	38.0	37.0	38.0	31.0	38.0
70-71	35.87400880383838	38.0	37.0	38.0	31.0	38.0
72-73	35.844096304379846	38.0	37.0	38.0	31.0	38.0
74-75	35.863156559761634	38.0	37.0	38.0	31.0	38.0
76-77	35.84246021453666	38.0	37.0	38.0	31.0	38.0
78-79	35.82475838234363	38.0	37.0	38.0	31.0	38.0
80-81	35.78409224675132	38.0	37.0	38.0	31.0	38.0
82-83	35.7858580274016	38.0	37.0	38.0	31.0	38.0
84-85	35.78075380047862	38.0	37.0	38.0	31.0	38.0
86-87	35.774830340279294	38.0	37.0	38.0	31.0	38.0
88-89	35.74741858887391	38.0	37.0	38.0	31.0	38.0
90-91	35.72740726650174	38.0	37.0	38.0	31.0	38.0
92-93	35.679854140654456	38.0	36.0	38.0	30.0	38.0
94-95	35.67143147587336	38.0	36.0	38.0	30.0	38.0
96-97	35.704142559706966	38.0	36.0	38.0	31.0	38.0
98-99	35.740201101792955	38.0	36.0	38.0	31.0	38.0
100-101	35.203033617470936	38.0	35.0	38.0	28.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	22.0
20	131.0
21	764.0
22	3287.0
23	9644.0
24	21503.0
25	39680.0
26	64072.0
27	94557.0
28	131916.0
29	174018.0
30	220828.0
31	275379.0
32	341716.0
33	434504.0
34	579204.0
35	854337.0
36	1643204.0
37	4757947.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.84520661277821	18.505363016246875	14.377533828348026	23.271896542626884
2	14.335675028095048	20.397716653240682	43.055798153921174	22.210810164743094
3	18.12251249860441	26.534371045512263	30.427253390020002	24.91586306586332
4	12.172068487134016	19.421114976214188	38.28433030636226	30.122486230289535
5	14.075022187336938	37.145942530622776	35.102332447361015	13.676702834679267
6	29.328651395080836	38.316393027804175	18.71275999907533	13.64219557803966
7	26.023589165101452	33.265959807880755	22.702034277568213	18.00841674944958
8	23.970217017872507	37.1186902238347	20.93952792437054	17.971564833922255
9	25.065190572087893	17.422590504106218	21.163303536322253	36.348915387483636
10-11	23.881259292669206	27.06662276917629	28.77974962881154	20.272368309342962
12-13	24.938111069289167	24.5195800809747	28.856273071968424	21.686035777767714
14-15	22.040255873591807	25.88692743276948	26.653540611460908	25.419276082177806
16-17	21.499889547915625	29.107700578341834	27.3223522572498	22.07005761649274
18-19	21.45922286307352	27.9482594959508	29.33208779733043	21.260429843645255
20-21	22.254868671069705	26.996257119137084	29.371359702248906	21.37751450754431
22-23	21.920130719916447	27.108919751585713	29.219049891966968	21.751899636530872
24-25	21.80451006979191	27.24037588857433	29.34470426444134	21.61040977719242
26-27	21.746264899875143	27.379637093993214	29.29238213499098	21.581715871140666
28-29	21.451604660869332	27.268041491168976	29.45558080710477	21.824773040856925
30-31	21.974928424467798	27.021050838871002	29.53024439220904	21.473776344452162
32-33	21.582571789369894	27.400015052353773	29.30668816883952	21.71072498943681
34-35	21.690761051874002	27.44422684939223	29.479212939541092	21.385799159192675
36-37	21.898953953385856	27.25434223007995	29.23711134049857	21.60959247603563
38-39	21.575477179300254	27.27608624186319	29.406405801392992	21.742030777443567
40-41	21.84056030159312	27.160246438137452	29.35104935054958	21.64814390971985
42-43	21.9373604530901	27.140917534903654	29.181661480275	21.74006053173125
44-45	21.774349847264425	27.44566611962783	28.955875641334593	21.824108391773148
46-47	22.07247158604583	27.25216646020769	28.549936045465152	22.125425908281333
48-49	21.9411889503068	27.349610782685964	28.575228225959403	22.133972041047837
50-51	21.794272910562547	27.581315185778276	28.539503835493306	22.084908068165866
52-53	21.986583650726395	27.546221680172284	28.58527587254585	21.881918796555468
54-55	21.956952744241903	27.488951662882418	28.421261784242034	22.13283380863365
56-57	22.09323910946385	27.731813747533945	28.162710476021235	22.012236666980968
58-59	21.794821518586605	27.947465175090358	28.49424132351292	21.763471982810117
60-61	22.032796523353706	28.101329539050866	28.123099823097697	21.742774114497735
62-63	21.815714356052965	28.29254901038716	28.067944137918754	21.823792495641122
64-65	21.785416490126465	28.260520665730247	28.035770671617367	21.918292172525923
66-67	21.895253587139482	28.221822591546726	27.959840577774752	21.923083243539036
68-69	21.877073109393226	28.027137163186655	28.131541786866265	21.964247940553854
70-71	21.942730530649087	27.620496735696598	28.25045215423621	22.186320579418105
72-73	22.057255560318183	27.54542513463432	28.241853709352206	22.155465595695286
74-75	21.89655048435744	27.712912462571026	28.191163985269423	22.199373067802107
76-77	22.019165165073783	27.665511286975764	28.104553065037052	22.210770482913397
78-79	22.009242289232265	27.705008708155287	28.0535184491383	22.23223055347415
80-81	22.038493185978087	27.6609523824151	28.071877343381935	22.228677088224877
82-83	22.09461451662463	27.681332258875347	28.101675548720394	22.122377675779628
84-85	22.258410270279253	27.63411809606949	28.009241723120677	22.098229910530584
86-87	22.014321403064592	27.717387133811528	28.098033420135327	22.170258042988554
88-89	22.132858962464105	27.707314637353882	27.9916386243932	22.168187775788812
90-91	22.144183634181207	27.75649746969578	28.010415575414665	22.088903320708354
92-93	22.176241722257636	27.710808152501848	27.98724242420291	22.1257077010376
94-95	22.023772620451272	27.824326206570028	27.988390594040276	22.16351057893843
96-97	22.038111533212316	27.83279573786309	28.03760363895446	22.091489089970132
98-99	22.315969609662318	28.383874665263264	28.082149587781636	21.218006137292782
100-101	22.915689689209252	30.885991042263406	25.43344366808118	20.764875600446167
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2675.0
1	2477.0
2	3061.0
3	4060.5
4	5111.0
5	7768.5
6	10319.5
7	12745.5
8	15487.5
9	16681.5
10	15678.0
11	14243.5
12	14805.5
13	17992.5
14	25366.5
15	35172.0
16	43788.5
17	50085.5
18	52219.5
19	49471.5
20	44758.5
21	40483.5
22	38877.0
23	41995.5
24	50038.5
25	63484.5
26	81677.5
27	102034.0
28	122510.5
29	142607.0
30	161242.5
31	181032.0
32	203472.0
33	227101.0
34	250970.5
35	272902.0
36	295913.5
37	317325.5
38	332038.0
39	343341.0
40	358279.5
41	372462.5
42	381328.5
43	391541.5
44	414210.0
45	429691.0
46	422723.5
47	414672.0
48	406652.5
49	395091.0
50	383879.5
51	372179.5
52	356811.5
53	338078.5
54	316677.0
55	292204.0
56	267542.0
57	240476.0
58	211822.5
59	183858.5
60	154031.5
61	124165.5
62	96545.0
63	73547.5
64	55843.5
65	41541.0
66	30012.5
67	22303.0
68	16808.5
69	12035.0
70	8657.5
71	6219.0
72	4331.0
73	2980.0
74	2002.0
75	1302.0
76	840.0
77	528.0
78	346.5
79	210.5
80	129.5
81	95.0
82	65.0
83	41.5
84	28.0
85	20.0
86	11.5
87	7.5
88	3.0
89	1.0
90	1.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0024878930313805203
4	0.0
5	0.05137499109800775
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	5.183110482042751E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	9.230258654663873E-4
28-29	2.127109197381518E-4
30-31	0.0
32-33	0.0
34-35	4.697420364632558E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	5.385123574584716E-6
46-47	6.523472459611686E-5
48-49	0.0
50-51	5.532745888505875E-6
52-53	5.575880258640551E-6
54-55	0.0010226845480624932
56-57	0.0019549000589926505
58-59	0.008431193770725376
60-61	0.00737158772059606
62-63	0.004021448111243381
64-65	0.004832783914897905
66-67	0.006371167489154333
68-69	0.00341344613423026
70-71	0.0053798966015448195
72-73	9.946775540878945E-4
74-75	0.0015125093199088626
76-77	0.001308574191335586
78-79	0.0010695221685049262
80-81	4.479581141244969E-5
82-83	0.0
84-85	0.0
86-87	3.7707258442175556E-4
88-89	8.366928223536476E-4
90-91	0.0
92-93	1.3724610071818826E-5
94-95	7.64544326368682E-5
96-97	2.7503223518838583E-4
98-99	1.4580314752544721E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	160.0
20-21	580.0
22-23	1407.0
24-25	1718.0
26-27	3738.0
28-29	7831.0
30-31	19122.0
32-33	24856.0
34-35	33878.0
36-37	51778.0
38-39	62073.0
40-41	61929.0
42-43	71887.0
44-45	86084.0
46-47	85781.0
48-49	78762.0
50-51	71193.0
52-53	67916.0
54-55	73051.0
56-57	76362.0
58-59	77826.0
60-61	78297.0
62-63	75785.0
64-65	84609.0
66-67	92919.0
68-69	93203.0
70-71	101262.0
72-73	77326.0
74-75	78999.0
76-77	83958.0
78-79	86899.0
80-81	88300.0
82-83	83541.0
84-85	84045.0
86-87	87403.0
88-89	90393.0
90-91	93043.0
92-93	91375.0
94-95	97989.0
96-97	152472.0
98-99	790557.0
100-101	6176410.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.85273586059498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.79423261124738	76.19286301566997
2	7.09998951745201	12.049070702746972
3	1.7399783041052397	4.4292575832422365
4	0.6163434008142623	2.0919369515485364
5	0.27760328222857955	1.177769899048793
6	0.15013620114635903	0.7643680451391095
7	0.0905789936456744	0.5380112795634444
8	0.054483159845482375	0.36984361369754326
9	0.03980012210169163	0.30394343231228405
>10	0.1336517784440455	1.7577267556463532
>50	0.0025028223315744233	0.14507632975778972
>100	5.778231984863707E-4	0.08207848070705606
>500	6.0993313696428626E-5	0.029086025426963313
>1k	6.099012549854706E-5	0.06896788549292919
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0366220964085501E-5	0.0
5	0.0	1.0366220964085501E-5	0.0	2.0732441928171003E-5	0.0
6	0.0	1.0366220964085501E-5	0.0	2.0732441928171003E-5	0.0
7	0.0	1.0366220964085501E-5	0.0	3.109866289225651E-5	0.0
8	0.0	1.0366220964085501E-5	0.0	3.109866289225651E-5	0.0
9	0.0	6.219732578451302E-5	0.0	5.183110482042751E-5	0.0
10-11	0.0	6.219732578451302E-5	0.0	5.183110482042751E-5	0.0
12-13	0.0	6.219732578451302E-5	0.0	5.701421530247026E-5	0.0
14-15	0.0	7.774665723064126E-5	0.0	8.811287819472676E-5	0.0
16-17	0.0	8.292976771268401E-5	0.0	1.2957776205106876E-4	0.0
18-19	0.0	8.811287819472676E-5	0.0	1.6585953542536802E-4	0.0
20-21	0.0	9.329598867676952E-5	0.0	1.8659197735353903E-4	0.0
22-23	0.0	1.1402843060494053E-4	0.0	2.1769064024579555E-4	0.0
24-25	0.0	1.2957776205106876E-4	0.0	2.539724136200948E-4	0.0
26-27	0.0	1.45127093497197E-4	0.0	3.680008442250353E-4	0.0
28-29	0.0	1.45127093497197E-4	0.0	7.204523570039424E-4	0.0
30-31	0.0	1.45127093497197E-4	0.0	0.0017570744534124926	0.0
32-33	0.0	1.45127093497197E-4	0.0	0.0038510510881577644	0.0
34-35	0.0	1.5031020397923977E-4	0.0	0.006670663190389021	0.0
36-37	0.0	1.5549331446128252E-4	0.0	0.01092599689614612	0.0
38-39	0.0	1.5549331446128252E-4	0.0	0.01723384235279215	5.183110482042751E-6
40-41	0.0	1.8659197735353903E-4	0.0	0.02458867612681081	1.0366220964085501E-5
42-43	0.0	1.9695819831762454E-4	0.0	0.031456297515517456	1.554933144612825E-5
44-45	0.0	2.228737507278383E-4	0.0	0.04000324670040595	2.5915552410213755E-5
46-47	0.0	2.3842308217396656E-4	0.0	0.04917216914313958	3.109866289225651E-5
48-49	0.0	2.3842308217396656E-4	0.0	0.05807675295128902	3.109866289225651E-5
50-51	0.0	2.6952174506622307E-4	0.0	0.06718866117872019	3.109866289225651E-5
52-53	0.0	2.6952174506622307E-4	0.0	0.07636276673193584	3.109866289225651E-5
54-55	0.0	2.6952174506622307E-4	0.0	0.08531399853442367	3.109866289225651E-5
56-57	0.0	2.798879660303086E-4	0.0	0.0942652303369115	3.109866289225651E-5
58-59	0.0	2.798879660303086E-4	0.0	0.10239234757275455	3.109866289225651E-5
60-61	1.0366220964085501E-5	2.798879660303086E-4	0.0	0.11030177416835178	3.628177337429925E-5
62-63	1.0366220964085501E-5	2.798879660303086E-4	0.0	0.11778618570442151	4.1464883856342006E-5
64-65	1.0366220964085501E-5	3.1098662892256503E-4	0.0	0.12465380709312816	4.1464883856342006E-5
66-67	1.0366220964085501E-5	3.1098662892256503E-4	0.0	0.13144368182460417	4.1464883856342006E-5
68-69	1.0366220964085501E-5	3.1098662892256503E-4	0.0	0.1382853876609006	4.1464883856342006E-5
70-71	1.0366220964085501E-5	3.1098662892256503E-4	0.0	0.1442148660523575	4.1464883856342006E-5
72-73	1.0366220964085501E-5	3.1098662892256503E-4	0.0	0.1486153268516118	4.1464883856342006E-5
74-75	1.0366220964085501E-5	3.161697394046078E-4	0.0	0.15229533529386216	4.1464883856342006E-5
76-77	1.0366220964085501E-5	3.265359603686933E-4	0.0	0.1556850895491181	4.1464883856342006E-5
78-79	1.0366220964085501E-5	3.472684022968643E-4	0.0	0.15891416737943076	4.1464883856342006E-5
80-81	1.0366220964085501E-5	3.5245151277890705E-4	0.0	0.16011664901126466	4.1464883856342006E-5
82-83	1.0366220964085501E-5	3.5245151277890705E-4	0.0	0.16075935471103797	4.1464883856342006E-5
84-85	1.0366220964085501E-5	3.8355017567116357E-4	0.0	0.16144870840514963	4.1464883856342006E-5
86-87	1.0366220964085501E-5	3.8355017567116357E-4	0.0	0.1616871314873236	4.1464883856342006E-5
88-89	1.0366220964085501E-5	3.990995071172918E-4	0.0	0.16175451192359017	5.183110482042751E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14260	0.0	51.89733	1
GTATCAA	26350	0.0	38.55271	1
CTTATAC	9425	0.0	33.21673	1
TCAACGC	31190	0.0	31.937885	4
ATCAACG	31290	0.0	31.836475	3
CAACGCA	31800	0.0	31.325241	5
TATCAAC	32160	0.0	31.1883	2
AACGCAG	32820	0.0	30.398653	6
ACGCAGA	36570	0.0	27.258074	7
TATACAC	13085	0.0	27.002321	3
CGCAGAG	37235	0.0	26.817238	8
TTATACA	14070	0.0	23.803097	2
TGGTATC	6195	0.0	23.435772	2
GCAGAGT	43490	0.0	22.970058	9
GTGGTAT	6670	0.0	22.794147	1
ACATCTC	14715	0.0	21.989338	8
GAGTACT	27885	0.0	21.289057	12-13
ACACATC	15030	0.0	20.901995	6
CAGAGTA	42305	0.0	20.568201	10-11
TACACAT	17220	0.0	19.846392	5
>>END_MODULE