FastQCFastQC Report
Fri 27 May 2016
SRR2139707_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139707_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9359195
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152050.053.099291
GTATCAA283700.039.0987971
TCAACGC348400.031.1994954
ATCAACG349150.031.1458683
TATCAAC355650.030.828742
CAACGCA358750.030.3112875
AACGCAG367600.029.598666
TGGTATC61600.028.0710012
GTGGTAT66150.027.882871
ACGCAGA411000.026.4316317
CGCAGAG421200.025.872598
GCAGAGT477200.022.8185279
GTACATG338900.021.3037281
GAGTACT289100.021.28997412-13
TACATGG337550.020.819742
ACATGGG335000.020.5321433
CAGAGTA471550.020.00637210-11
GTACTTT320600.019.1188214-15
AGAGTAC443150.018.9584310-11
CATGGGG213750.017.7966774
Produced by FastQC (version 0.11.5)