FastQCFastQC Report
Fri 27 May 2016
SRR2139706_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139706_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9768594
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141350.051.605821
GTATCAA247250.035.2632941
TCAACGC302750.029.254614
ATCAACG303250.029.1783583
TATCAAC312650.028.494372
CAACGCA313150.028.2968245
AACGCAG323450.027.460756
GTGGTAT64400.024.7313751
TGGTATC63600.024.1602082
ACGCAGA373850.023.7471337
CGCAGAG377600.023.488458
CTTATAC129950.022.0546191
GAGTACT261250.020.33486612-13
GCAGAGT439700.020.2692229
CAGAGTA433200.019.01989610-11
TATACAC172600.019.0055583
GTACTTT286650.018.4280314-15
TACATGG299950.017.8291322
GTACATG295950.017.778421
TTATACA171100.017.608132
Produced by FastQC (version 0.11.5)