##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139706_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9768594
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.35929960852094	32.0	25.0	33.0	18.0	33.0
2	30.188625507416933	32.0	29.0	33.0	25.0	33.0
3	31.61441114248376	33.0	32.0	33.0	27.0	34.0
4	31.76359801625495	33.0	32.0	33.0	30.0	34.0
5	32.139675474280125	33.0	33.0	34.0	31.0	34.0
6	35.61387278455835	38.0	36.0	38.0	30.0	38.0
7	35.96447503090005	38.0	37.0	38.0	31.0	38.0
8	36.31503100650923	38.0	37.0	38.0	33.0	38.0
9	36.44547997388365	38.0	37.0	38.0	34.0	38.0
10-11	36.552934076285695	38.0	38.0	38.0	34.0	38.0
12-13	36.576016108357045	38.0	38.0	38.0	34.0	38.0
14-15	36.59883766282026	38.0	38.0	38.0	34.0	38.0
16-17	36.60161641480852	38.0	38.0	38.0	34.0	38.0
18-19	36.61426229813625	38.0	38.0	38.0	34.0	38.0
20-21	36.610108274582885	38.0	38.0	38.0	34.0	38.0
22-23	36.60747507024652	38.0	38.0	38.0	34.0	38.0
24-25	36.60847867753037	38.0	38.0	38.0	34.0	38.0
26-27	36.55550162976354	38.0	38.0	38.0	34.0	38.0
28-29	36.52719780232515	38.0	38.0	38.0	34.0	38.0
30-31	36.526430371771696	38.0	38.0	38.0	34.0	38.0
32-33	36.5037668010087	38.0	38.0	38.0	34.0	38.0
34-35	36.46245262790934	38.0	38.0	38.0	34.0	38.0
36-37	36.44326072468789	38.0	38.0	38.0	34.0	38.0
38-39	36.41504774851464	38.0	38.0	38.0	34.0	38.0
40-41	36.43325554791053	38.0	38.0	38.0	34.0	38.0
42-43	36.39049398621015	38.0	38.0	38.0	34.0	38.0
44-45	36.34012274407438	38.0	38.0	38.0	33.0	38.0
46-47	36.3013994306753	38.0	38.0	38.0	33.0	38.0
48-49	36.25427892557764	38.0	38.0	38.0	33.0	38.0
50-51	36.22694336606304	38.0	37.0	38.0	33.0	38.0
52-53	36.2068254702169	38.0	37.0	38.0	33.0	38.0
54-55	36.16833133129233	38.0	37.0	38.0	33.0	38.0
56-57	36.16685944628779	38.0	37.0	38.0	33.0	38.0
58-59	36.141230175750735	38.0	37.0	38.0	33.0	38.0
60-61	36.12645728899335	38.0	37.0	38.0	33.0	38.0
62-63	36.123843693746636	38.0	37.0	38.0	33.0	38.0
64-65	36.111042741283	38.0	37.0	38.0	33.0	38.0
66-67	36.11402930920195	38.0	37.0	38.0	33.0	38.0
68-69	36.096701548908925	38.0	37.0	38.0	33.0	38.0
70-71	36.066506189991514	38.0	37.0	38.0	32.5	38.0
72-73	36.03977000871687	38.0	37.0	38.0	31.5	38.0
74-75	36.058459896655876	38.0	37.0	38.0	32.5	38.0
76-77	36.03834331249584	38.0	37.0	38.0	31.5	38.0
78-79	36.02362015982011	38.0	37.0	38.0	31.0	38.0
80-81	35.982344526480375	38.0	37.0	38.0	31.0	38.0
82-83	35.985853640871014	38.0	37.0	38.0	31.0	38.0
84-85	35.98393826056292	38.0	37.0	38.0	31.0	38.0
86-87	35.98292552312479	38.0	37.0	38.0	31.0	38.0
88-89	35.956086023212976	38.0	37.0	38.0	31.0	38.0
90-91	35.937920627768634	38.0	37.0	38.0	31.0	38.0
92-93	35.88921553849296	38.0	37.0	38.0	31.0	38.0
94-95	35.88220184893828	38.0	37.0	38.0	31.0	38.0
96-97	35.90764312043251	38.0	37.0	38.0	31.0	38.0
98-99	35.939569547583424	38.0	37.0	38.0	31.0	38.0
100-101	35.413833884496796	38.0	36.0	38.0	29.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	16.0
19	51.0
20	119.0
21	529.0
22	2359.0
23	7296.0
24	17238.0
25	33120.0
26	54509.0
27	83691.0
28	117782.0
29	157288.0
30	201855.0
31	254592.0
32	319024.0
33	407891.0
34	547119.0
35	817722.0
36	1621631.0
37	5124761.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.115608039396456	23.06333951436614	14.545276423608147	24.275776022629255
2	15.035111501204778	21.284762167411195	41.27095465324898	22.40917167813505
3	18.138214237709143	26.82327803438449	30.22820042670684	24.810307301199522
4	13.037045044558102	18.899065720204973	38.39492152094764	29.668967714289284
5	13.553302154348879	37.21995195533038	35.76300257266567	13.463743317655075
6	29.257137721150045	38.55521070893109	18.636172206563195	13.551479363355668
7	25.8682672245361	33.08958279973556	23.010926649218916	18.031223326509423
8	23.60662138277013	37.72366831910508	21.05913092508502	17.610579373039766
9	24.922972538320252	17.429908541597698	21.451306093794052	36.195812826288
10-11	23.797959051220676	27.30125747881425	28.796078534945767	20.104704935019306
12-13	24.727320021694013	24.675255210729404	29.05933545810175	21.538089309474834
14-15	21.87460364046286	26.176364395568534	26.65804072295775	25.290991241010857
16-17	21.319726257432748	29.41071151078651	27.459509526140607	21.81005270564014
18-19	21.305220587527746	28.206423565151752	29.403479149609453	21.084876697711053
20-21	22.115976695778684	27.24477570900087	29.393678098849563	21.245569496370884
22-23	21.829839531881778	27.418793987757716	29.2365088682309	21.514857612129607
24-25	21.625052768866784	27.595898040361643	29.346211786662955	21.432837404108614
26-27	21.59223526664837	27.759879241202178	29.249205318630363	21.398680173519086
28-29	21.400313148978924	27.58216267847689	29.342944436340034	21.67457973620415
30-31	21.917360631030938	27.513232097626624	29.283079628834763	21.286327642507676
32-33	21.695622004673346	27.616428876120153	29.29818330433382	21.389765814872682
34-35	21.731560404427537	27.675669562142446	29.352047701160405	21.24072233226961
36-37	21.665513355958385	27.54136990564738	29.289900953142155	21.50321578525208
38-39	21.4747425187784	27.506896811473325	29.444923319288357	21.573437350459916
40-41	21.678964454905426	27.37418742460951	29.40560759900614	21.54124052147893
42-43	21.68345404079732	27.412941091217647	29.265150697466414	21.638454170518617
44-45	21.56731314171688	27.640111896928243	29.088397215854428	21.704177745500452
46-47	21.86952809388332	27.487716344731677	28.667083120390252	21.97567244099475
48-49	21.741109908196936	27.61557260977911	28.671255246188093	21.972062235835864
50-51	21.618140865401713	27.777183198808032	28.69699323952835	21.907682696261908
52-53	21.765463422840313	27.765531655278984	28.712757806768472	21.75624711511223
54-55	21.712551777168628	27.73020830353754	28.567609985682036	21.989629933611802
56-57	21.863072051116777	27.909260393292644	28.340899499248472	21.886768056342106
58-59	21.617545223977277	28.099896452671093	28.605994923028177	21.676563400323452
60-61	21.82762943466694	28.225666546389995	28.329040233844005	21.61766378509906
62-63	21.619472958653414	28.459501924350388	28.296369564833196	21.624655552163002
64-65	21.596343565482083	28.428606465691132	28.24114314005941	21.733906828767374
66-67	21.634184958147717	28.461213011844578	28.1284439263061	21.776158103701608
68-69	21.646741068790096	28.299497259829604	28.204916704046223	21.848844967334077
70-71	21.717889911917403	27.92620764742041	28.316615946748847	22.039286493913345
72-73	21.74519660180911	27.860160425332282	28.287206794948403	22.107436177910206
74-75	21.730248351066727	27.91468207390325	28.239316941774085	22.115752633255934
76-77	21.907491274439884	27.836304958896168	28.189888475935902	22.066315290728042
78-79	21.871348657085864	27.86748214540725	28.081764898277573	22.179404299229308
80-81	21.9058384687338	27.932503162665256	28.077330392766285	22.084327975834654
82-83	21.896508297315503	27.933383206828093	28.147499387240728	22.022609108615683
84-85	22.020317400965624	27.96608505664925	28.059804866716853	21.953792675668275
86-87	21.917043245802155	27.968426663472712	28.07766228476542	22.036867805959712
88-89	21.971369221136708	27.994188689621417	28.028191037581195	22.006251051660676
90-91	21.970914151881864	28.043847216890093	28.039556805762757	21.94568182546529
92-93	22.0252261647019	28.00883749490501	28.06051526909497	21.905421071298125
94-95	21.984074209662435	28.06769907037069	28.07979713283284	21.868429587134035
96-97	21.981231049861428	28.055800608575982	28.089384936522972	21.873583405039618
98-99	22.183119931656158	28.5501309262677	28.236059223388242	21.030689918687898
100-101	22.686754833938135	31.11562982258857	25.669159152881587	20.52845619059171
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2428.0
1	2331.0
2	2835.5
3	3577.5
4	4475.0
5	6297.0
6	7934.5
7	9983.0
8	12140.5
9	13246.5
10	13183.5
11	12807.5
12	13883.0
13	17444.0
14	25463.0
15	35559.5
16	43122.5
17	47100.0
18	47522.0
19	44814.5
20	40332.5
21	36270.5
22	34955.5
23	37977.5
24	45862.5
25	58536.0
26	74884.0
27	93876.0
28	115858.0
29	140034.5
30	165052.5
31	190823.0
32	217381.0
33	243962.5
34	269777.0
35	293918.0
36	319091.5
37	340924.5
38	357351.0
39	371517.0
40	383327.0
41	394501.5
42	401273.0
43	406555.0
44	413170.5
45	425891.0
46	431410.0
47	422882.5
48	414471.0
49	400481.0
50	384106.0
51	367178.5
52	345286.0
53	322601.5
54	299569.0
55	274606.0
56	248231.5
57	221226.0
58	194278.0
59	165363.5
60	135737.5
61	109490.0
62	85857.0
63	65164.0
64	48512.0
65	35739.0
66	26386.5
67	20029.5
68	15183.5
69	11258.0
70	8306.0
71	5885.0
72	4113.0
73	2847.0
74	1884.0
75	1196.0
76	741.5
77	431.5
78	282.5
79	186.5
80	108.0
81	63.5
82	45.5
83	38.5
84	29.5
85	23.0
86	18.0
87	12.0
88	6.5
89	3.0
90	2.0
91	2.0
92	2.5
93	2.5
94	2.0
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0030198818785999297
4	0.0
5	0.05302707841066995
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.0236887724067558E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.602147013969478E-4
28-29	2.458635506466621E-4
30-31	0.0
32-33	0.0
34-35	9.270513108994813E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	1.0698405841244002E-4
48-49	0.0
50-51	1.6314664191188285E-5
52-53	1.0952236148173022E-5
54-55	0.0011959510059105331
56-57	0.0022641103602859948
58-59	0.009021286547249575
60-61	0.008126751168347286
62-63	0.004415238334224337
64-65	0.005374798201539806
66-67	0.006941970162370947
68-69	0.003910743390814441
70-71	0.005697944738329276
72-73	0.0010630805098940247
74-75	0.0016630052432868212
76-77	0.0013379084125065975
78-79	0.0012038557041355086
80-81	1.0550481291234928E-4
82-83	0.0
84-85	0.0
86-87	2.6872159348833837E-4
88-89	9.635414662656697E-4
90-91	0.0
92-93	6.616872057683773E-6
94-95	1.1381672815336442E-4
96-97	4.139493886476484E-4
98-99	1.4012923699140006E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	139.0
20-21	512.0
22-23	1042.0
24-25	1367.0
26-27	2812.0
28-29	6080.0
30-31	17859.0
32-33	23437.0
34-35	32203.0
36-37	50128.0
38-39	56583.0
40-41	59365.0
42-43	67998.0
44-45	81121.0
46-47	81912.0
48-49	74944.0
50-51	66403.0
52-53	57394.0
54-55	61054.0
56-57	62895.0
58-59	72527.0
60-61	73850.0
62-63	71790.0
64-65	81558.0
66-67	88908.0
68-69	87670.0
70-71	96438.0
72-73	74033.0
74-75	74924.0
76-77	80163.0
78-79	84102.0
80-81	82994.0
82-83	79835.0
84-85	79500.0
86-87	81753.0
88-89	86322.0
90-91	88883.0
92-93	87499.0
94-95	93953.0
96-97	149864.0
98-99	811263.0
100-101	6435517.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.37977567358342
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.44453297076446	78.12578468916655
2	6.889663460486589	11.902551683666323
3	1.541063313902408	3.9935011006103722
4	0.5218086770060195	1.8029486585723724
5	0.2321216490680224	1.0025307987739014
6	0.12871483978737344	0.6671015392016728
7	0.06465068945443392	0.3909158436551556
8	0.04120308399743662	0.28472905222067124
9	0.02942136785402309	0.2287270039716444
>10	0.10402660669162912	1.372293740317624
>50	0.00229217325041856	0.1314510787066634
>100	4.4627616872769876E-4	0.06746694009399123
>500	5.489156851042613E-5	0.029997871043067538
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0236887724067558E-5
2	0.0	3.0710663172202675E-5	0.0	0.0	1.0236887724067558E-5
3	0.0	5.118443862033779E-5	0.0	0.0	1.0236887724067558E-5
4	0.0	1.1260576496474313E-4	0.0	0.0	2.0473775448135116E-5
5	0.0	1.1260576496474313E-4	0.0	1.0236887724067558E-5	2.0473775448135116E-5
6	1.0236887724067558E-5	1.1260576496474313E-4	0.0	1.0236887724067558E-5	5.118443862033779E-5
7	1.0236887724067558E-5	1.228426526888107E-4	0.0	1.0236887724067558E-5	5.118443862033779E-5
8	1.0236887724067558E-5	1.228426526888107E-4	0.0	1.0236887724067558E-5	6.142132634440535E-5
9	1.0236887724067558E-5	1.5355331586101335E-4	0.0	8.189510179254046E-5	6.142132634440535E-5
10-11	1.0236887724067558E-5	1.5355331586101335E-4	0.0	9.725043337864179E-5	6.142132634440535E-5
12-13	1.0236887724067558E-5	1.6379020358508092E-4	0.0	1.0748732110270936E-4	6.142132634440535E-5
14-15	1.0236887724067558E-5	2.508037492396552E-4	0.0	1.1260576496474313E-4	7.677665793050668E-5
16-17	2.5592219310168894E-5	2.610406369637227E-4	0.0	1.689086474471147E-4	8.189510179254046E-5
18-19	4.094755089627023E-5	2.815144124118578E-4	0.0	1.9961931061931738E-4	8.701354565457425E-5
20-21	4.094755089627023E-5	3.071066317220267E-4	0.0	2.0985619834338492E-4	9.213198951660802E-5
22-23	4.094755089627023E-5	3.429357387562632E-4	0.0	2.3544841765355383E-4	1.0236887724067557E-4
24-25	5.118443862033779E-5	3.9412017737660096E-4	0.0	2.7639596854982403E-4	1.1260576496474313E-4
26-27	5.118443862033779E-5	4.1971239668676984E-4	0.0	3.7364640192846587E-4	1.1260576496474313E-4
28-29	5.118443862033779E-5	4.1971239668676984E-4	0.0	6.858714775125264E-4	1.1260576496474313E-4
30-31	6.653977020643913E-5	4.40186172134905E-4	0.0	0.0020013115500552075	1.1260576496474313E-4
32-33	7.16582140684729E-5	4.606599475830401E-4	0.0	0.004668020802174806	1.3307954041287824E-4
34-35	8.189510179254046E-5	5.016074984793104E-4	0.0	0.00861434101980285	1.3307954041287824E-4
36-37	9.213198951660802E-5	5.425550493755806E-4	0.0	0.014782065873553553	1.433164281369458E-4
38-39	9.213198951660802E-5	5.527919370996482E-4	0.0	0.026887185607263442	1.433164281369458E-4
40-41	1.0236887724067557E-4	6.346870388921886E-4	0.0	0.06815208002297976	1.433164281369458E-4
42-43	1.0236887724067557E-4	6.602792582023574E-4	0.0	0.07954573605986695	1.4843487199897958E-4
44-45	1.0236887724067557E-4	7.472928038569317E-4	0.0	0.0969023791960235	1.5355331586101335E-4
46-47	1.0236887724067557E-4	7.984772424772695E-4	0.0	0.11665445405961186	1.6379020358508092E-4
48-49	1.0236887724067557E-4	7.984772424772695E-4	0.0	0.13454341535741993	1.740270913091485E-4
50-51	1.0236887724067557E-4	8.957276758559113E-4	0.0	0.15226858645164287	1.945008667572836E-4
52-53	1.0236887724067557E-4	9.00846119717945E-4	0.0	0.18437658479818078	2.1497464220541872E-4
54-55	1.0236887724067557E-4	9.26438339028114E-4	0.0	0.26819622148284594	2.3544841765355383E-4
56-57	1.0236887724067557E-4	9.622674460623504E-4	0.0	0.3621810876775102	2.456853053776214E-4
58-59	1.0236887724067557E-4	9.72504333786418E-4	0.0	0.46265102224537125	2.5592219310168894E-4
60-61	1.0236887724067557E-4	0.0010032149969586206	0.0	0.48519776745763005	2.610406369637227E-4
62-63	1.0236887724067557E-4	0.0010339256601308235	0.0	0.4991148163184999	2.763959685498241E-4
64-65	1.0236887724067557E-4	0.0011772420882677692	0.0	0.5096895213374617	2.763959685498241E-4
66-67	1.0236887724067557E-4	0.0011925974198538705	0.0	0.5158418908596263	2.815144124118579E-4
68-69	1.0236887724067557E-4	0.0012130711953020055	0.0	0.5220608001519973	2.866328562738916E-4
70-71	1.1260576496474313E-4	0.0012284265268881068	0.0	0.5278292863845093	2.9686974399795917E-4
72-73	1.1260576496474313E-4	0.0012335449707501407	0.0	0.5317756066021374	3.071066317220267E-4
74-75	1.1260576496474313E-4	0.0012591371900603095	0.0	0.5353124513108027	3.071066317220267E-4
76-77	1.1260576496474313E-4	0.0013256769602667487	0.0	0.5383630438525748	3.173435194460943E-4
78-79	1.1260576496474313E-4	0.0013615060673009853	0.0	0.541249846190762	3.2758040717016185E-4
80-81	1.1260576496474313E-4	0.0013615060673009853	0.0	0.5425141018246843	3.2758040717016185E-4
82-83	1.1260576496474313E-4	0.0013615060673009853	0.0	0.5431743810828866	3.5317262648033073E-4
84-85	1.1260576496474313E-4	0.0013717429550250528	0.0	0.5435889750357115	3.5829107034236453E-4
86-87	1.1260576496474313E-4	0.0013717429550250528	0.0	0.5437374099077104	3.6340951420439833E-4
88-89	1.1260576496474313E-4	0.0014229273936453904	0.0	0.5437732390147446	3.685279580664321E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14135	0.0	51.60582	1
GTATCAA	24725	0.0	35.263294	1
TCAACGC	30275	0.0	29.25461	4
ATCAACG	30325	0.0	29.178358	3
TATCAAC	31265	0.0	28.49437	2
CAACGCA	31315	0.0	28.296824	5
AACGCAG	32345	0.0	27.46075	6
GTGGTAT	6440	0.0	24.731375	1
TGGTATC	6360	0.0	24.160208	2
ACGCAGA	37385	0.0	23.747133	7
CGCAGAG	37760	0.0	23.48845	8
CTTATAC	12995	0.0	22.054619	1
GAGTACT	26125	0.0	20.334866	12-13
GCAGAGT	43970	0.0	20.269222	9
CAGAGTA	43320	0.0	19.019896	10-11
TATACAC	17260	0.0	19.005558	3
GTACTTT	28665	0.0	18.42803	14-15
TACATGG	29995	0.0	17.829132	2
GTACATG	29595	0.0	17.77842	1
TTATACA	17110	0.0	17.60813	2
>>END_MODULE