FastQCFastQC Report
Fri 27 May 2016
SRR2139705_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139705_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11108435
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153750.050.07951
GTATCAA271250.035.5939751
TCAACGC329900.029.6574524
ATCAACG332400.029.448443
TATCAAC343050.028.6726062
CAACGCA343000.028.5373445
AACGCAG355950.027.5579646
ACGCAGA409000.024.0046047
CGCAGAG414800.023.7625528
GTGGTAT73850.023.0263981
TGGTATC73900.022.4267862
GCAGAGT484500.020.3440829
GAGTACT279700.019.84097112-13
CAGAGTA477050.018.69959810-11
GTACTTT309400.017.90879214-15
AGAGTAC440300.017.89927710-11
TACATGG334050.016.8220832
GTACATG331850.016.8165511
ACATGGG339400.015.9210883
AGTACTT305000.015.84738612-13
Produced by FastQC (version 0.11.5)