##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139705_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11108435
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.114531885004503	32.0	25.0	33.0	18.0	33.0
2	30.071300232661038	32.0	28.0	33.0	25.0	33.0
3	31.559015288832317	33.0	32.0	33.0	27.0	34.0
4	31.727887321661424	33.0	32.0	33.0	30.0	34.0
5	32.10986047989658	33.0	33.0	34.0	31.0	34.0
6	35.58627385405775	38.0	36.0	38.0	30.0	38.0
7	35.972026662621694	38.0	37.0	38.0	31.0	38.0
8	36.313395181229396	38.0	37.0	38.0	33.0	38.0
9	36.450642687291236	38.0	37.0	38.0	34.0	38.0
10-11	36.55876111261397	38.0	38.0	38.0	34.0	38.0
12-13	36.5790937247236	38.0	38.0	38.0	34.0	38.0
14-15	36.60099991582973	38.0	38.0	38.0	34.0	38.0
16-17	36.59938726742336	38.0	38.0	38.0	34.0	38.0
18-19	36.60925665946643	38.0	38.0	38.0	34.0	38.0
20-21	36.60096173538607	38.0	38.0	38.0	34.0	38.0
22-23	36.59738821318713	38.0	38.0	38.0	34.0	38.0
24-25	36.596989877672996	38.0	38.0	38.0	34.0	38.0
26-27	36.542113544782595	38.0	38.0	38.0	34.0	38.0
28-29	36.51573031164535	38.0	38.0	38.0	34.0	38.0
30-31	36.51814257440825	38.0	38.0	38.0	34.0	38.0
32-33	36.50267916202421	38.0	38.0	38.0	34.0	38.0
34-35	36.46932125234075	38.0	38.0	38.0	34.0	38.0
36-37	36.45009936260476	38.0	38.0	38.0	34.0	38.0
38-39	36.43660304413402	38.0	38.0	38.0	34.0	38.0
40-41	36.43077458095823	38.0	38.0	38.0	34.0	38.0
42-43	36.379214910886986	38.0	38.0	38.0	33.5	38.0
44-45	36.33211799495786	38.0	38.0	38.0	33.0	38.0
46-47	36.295712700458665	38.0	38.0	38.0	33.0	38.0
48-49	36.250813659605186	38.0	37.5	38.0	33.0	38.0
50-51	36.22344605930273	38.0	37.0	38.0	33.0	38.0
52-53	36.2028865776181	38.0	37.0	38.0	33.0	38.0
54-55	36.166037614532705	38.0	37.0	38.0	33.0	38.0
56-57	36.16234601247592	38.0	37.0	38.0	33.0	38.0
58-59	36.13807205871461	38.0	37.0	38.0	33.0	38.0
60-61	36.121766654564325	38.0	37.0	38.0	33.0	38.0
62-63	36.11878115769785	38.0	37.0	38.0	33.0	38.0
64-65	36.107190441030276	38.0	37.0	38.0	33.0	38.0
66-67	36.10987286143617	38.0	37.0	38.0	33.0	38.0
68-69	36.094012635987646	38.0	37.0	38.0	33.0	38.0
70-71	36.062687177344756	38.0	37.0	38.0	32.5	38.0
72-73	36.03376240261689	38.0	37.0	38.0	31.5	38.0
74-75	36.05173081502974	38.0	37.0	38.0	32.0	38.0
76-77	36.030501112051695	38.0	37.0	38.0	31.5	38.0
78-79	36.01301938798801	38.0	37.0	38.0	31.0	38.0
80-81	35.97249916448601	38.0	37.0	38.0	31.0	38.0
82-83	35.971926413556005	38.0	37.0	38.0	31.0	38.0
84-85	35.96689630238887	38.0	37.0	38.0	31.0	38.0
86-87	35.960411554977014	38.0	37.0	38.0	31.0	38.0
88-89	35.93374141718084	38.0	37.0	38.0	31.0	38.0
90-91	35.916208865174426	38.0	37.0	38.0	31.0	38.0
92-93	35.86592632801268	38.0	37.0	38.0	31.0	38.0
94-95	35.85776305412956	38.0	37.0	38.0	31.0	38.0
96-97	35.8866107418037	38.0	37.0	38.0	31.0	38.0
98-99	35.91344319990674	38.0	37.0	38.0	31.0	38.0
100-101	35.39273348755556	38.0	35.5	38.0	29.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	10.0
19	26.0
20	105.0
21	659.0
22	2787.0
23	8712.0
24	20127.0
25	37572.0
26	63003.0
27	94791.0
28	133847.0
29	178393.0
30	228845.0
31	288359.0
32	363631.0
33	466243.0
34	626945.0
35	936236.0
36	1871123.0
37	5787020.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.1385316653516	23.03792568440109	14.536917216511597	24.286625433735715
2	15.291352922351347	21.20598446135752	40.82877561060582	22.67388700568532
3	18.331285579866528	26.5730401612948	29.929111240185996	25.16656301865268
4	13.286200981506397	18.61246881311364	38.17835725734543	29.92297294803453
5	13.898651101807799	37.00859896364974	35.44918283680325	13.64356709773921
6	29.796108074279417	38.293093337908836	18.39312363920963	13.517674948602115
7	26.278544187367526	32.94271425272777	22.88911084234638	17.889630717558326
8	23.96765160889	37.5405716466811	20.771521821030596	17.7202549233983
9	25.097423714501637	17.524268720121242	21.32127522913894	36.05703233623818
10-11	24.143792532431434	27.155193328313125	28.56230423097403	20.13870990828141
12-13	24.942410879660365	24.549560761709458	28.846907777738267	21.661120580891907
14-15	22.12260171517433	25.94757847719933	26.51808646106582	25.41173334656052
16-17	21.60541516424231	29.079388770785442	27.300483821528417	22.014712243443835
18-19	21.595143690357823	28.07359902632549	29.152877970659237	21.17837931265745
20-21	22.430720539161257	27.151442358005895	29.1079535585213	21.309883544311546
22-23	22.1462089666052	27.11724991036456	28.96846880607842	21.768072316951823
24-25	21.918300412901754	27.26555728770963	29.051313698905684	21.764828600482936
26-27	21.788854700639185	27.41832886924897	28.988647935148038	21.804168494963804
28-29	21.708153811650728	27.357849974773536	29.09191535673813	21.842080856837605
30-31	21.992484465718228	27.20598793740021	29.01197400829261	21.789553588588948
32-33	21.81826534370667	27.372380944035047	28.94364513127046	21.865708580987814
34-35	21.953110080046184	27.42099371791895	28.965571304724342	21.660324897310527
36-37	21.940386662848766	27.310474694418357	28.905846592149125	21.843292050583752
38-39	21.797066370072244	27.385871271413336	29.018295556002098	21.798766802512326
40-41	22.01724273810289	27.27937600161997	28.904792501263763	21.798588759013374
42-43	21.9853839320608	27.342457216643055	28.741347569666193	21.93081128162995
44-45	21.99953613756435	27.44274037805954	28.59811214981591	21.9596113345602
46-47	22.1308296392598	27.407773241064255	28.30045908832372	22.160938031352227
48-49	22.019197295983975	27.53184607583402	28.317393915214684	22.13156271296732
50-51	21.98089335686377	27.66145209990794	28.24724373969536	22.11041080353293
52-53	22.153562362755288	27.60837753610611	28.16698294740922	22.071077153729384
54-55	22.09118220152178	27.550980599973933	28.160881522513844	22.19695567599044
56-57	22.11871320912219	27.650677610518915	28.08189473457059	22.148714445788304
58-59	22.02193373004329	27.70094148383023	28.196811708301432	22.080313077825043
60-61	22.13789961522805	27.728239272732107	28.1316977061409	22.002163405898937
62-63	22.020557968864555	27.85058654935296	28.09980632797307	22.029049153809414
64-65	22.007167125161814	27.810463329160797	28.067798394430056	22.114571151247333
66-67	22.055796999146573	27.880973185134877	27.89933392093793	22.163895894780623
68-69	22.004295682461592	27.800487816529206	27.993641999580344	22.201574501428855
70-71	22.071439585930623	27.530340950575543	28.043449972116857	22.35476949137698
72-73	22.083098849116627	27.554285486190484	27.985024021503452	22.377591643189433
74-75	21.970025657859267	27.61363408107968	28.001266506608985	22.415073754452067
76-77	22.16195969711575	27.534896706942796	27.939564884586964	22.36357871135449
78-79	22.129531611352554	27.597646957607914	27.845860500388063	22.426960930651468
80-81	22.14534713319403	27.647084679661095	27.807895080418543	22.39967310672633
82-83	22.15826150940555	27.613238001591405	27.88477119897558	22.343729290027465
84-85	22.25672943468745	27.61631101087208	27.818922489724017	22.308037064716455
86-87	22.172380083753445	27.650318209269898	27.85097341061534	22.32632829636132
88-89	22.169386601046277	27.63267579455797	27.835882002591983	22.362055601803764
90-91	22.162371320769374	27.649768049425326	27.905723021944244	22.282137607861056
92-93	22.200893392111364	27.59587028109809	27.889440647602587	22.313795679187958
94-95	22.089217791354187	27.669158854638166	27.910549785143594	22.331073568864053
96-97	22.119733309838367	27.700508110506444	27.90388593375767	22.275872645897522
98-99	22.380330046068217	28.316245445896932	27.929085832017574	21.374338676017278
100-101	22.944399100629088	30.777895784186793	25.307303542228553	20.970401572955566
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3311.0
1	3089.0
2	3287.5
3	3875.5
4	4691.0
5	6488.5
6	8324.0
7	10746.5
8	13314.0
9	14602.0
10	14391.5
11	13860.0
12	14988.5
13	18396.0
14	26261.5
15	36198.5
16	43673.5
17	47776.0
18	48284.5
19	45516.0
20	41556.0
21	38303.0
22	37560.5
23	41592.0
24	50670.0
25	65133.0
26	84167.0
27	105523.5
28	128409.5
29	153576.0
30	179018.5
31	204623.5
32	232864.0
33	261904.0
34	289867.0
35	315763.0
36	343765.5
37	369743.5
38	388480.5
39	403944.0
40	420036.0
41	435561.5
42	445511.5
43	453823.5
44	466324.0
45	474604.0
46	472877.5
47	468881.0
48	463177.0
49	454474.0
50	444430.0
51	430900.5
52	411345.0
53	388843.0
54	365890.0
55	339109.0
56	309530.5
57	278377.0
58	246683.5
59	213769.5
60	178110.5
61	144200.5
62	113617.5
63	86985.0
64	65315.5
65	48398.5
66	35611.0
67	26694.5
68	20094.5
69	14648.5
70	10530.5
71	7566.5
72	5372.0
73	3667.5
74	2407.0
75	1577.0
76	1026.0
77	606.5
78	385.0
79	270.0
80	170.5
81	105.5
82	69.5
83	45.0
84	27.0
85	17.5
86	14.0
87	11.5
88	7.5
89	4.5
90	3.5
91	2.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.002943708992310798
4	0.0
5	0.05237461442588447
6	9.002168172204276E-6
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.3503252258306413E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.960358025288382E-4
28-29	2.567457698882255E-4
30-31	0.0
32-33	0.0
34-35	6.340180290272475E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	9.323867183936171E-6
46-47	1.0815122117540723E-4
48-49	0.0
50-51	4.301315566924623E-5
52-53	3.3692960765557974E-5
54-55	0.001095232448306361
56-57	0.0022750623628280194
58-59	0.008963225832508793
60-61	0.007911490902355883
62-63	0.0042754138600616534
64-65	0.005107014128481485
66-67	0.006832026464513296
68-69	0.003744392271779805
70-71	0.005515100245896685
72-73	0.0010214790324927765
74-75	0.0016174655836624558
76-77	0.0011899980186266174
78-79	0.0011427848178233895
80-81	5.9914420420577444E-5
82-83	0.0
84-85	0.0
86-87	3.5958577292147204E-4
88-89	9.656646570427769E-4
90-91	0.0
92-93	1.162862024385798E-5
94-95	1.2356677254181557E-4
96-97	3.042247149295117E-4
98-99	1.2324623686102681E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	123.0
20-21	545.0
22-23	1209.0
24-25	1506.0
26-27	3166.0
28-29	6950.0
30-31	19976.0
32-33	26181.0
34-35	36353.0
36-37	57201.0
38-39	64659.0
40-41	66842.0
42-43	76484.0
44-45	90797.0
46-47	91066.0
48-49	84071.0
50-51	76409.0
52-53	69270.0
54-55	74752.0
56-57	76754.0
58-59	82516.0
60-61	82994.0
62-63	79894.0
64-65	87372.0
66-67	93332.0
68-69	95032.0
70-71	96710.0
72-73	85466.0
74-75	87870.0
76-77	93582.0
78-79	95178.0
80-81	96961.0
82-83	92574.0
84-85	91955.0
86-87	95897.0
88-89	99612.0
90-91	102785.0
92-93	101098.0
94-95	108448.0
96-97	173094.0
98-99	931697.0
100-101	7310054.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.19816196841253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.86224424058378	76.56098039654303
2	7.088331651423685	12.078256562476405
3	1.6801848843326341	4.294459917367507
4	0.6443983586406915	2.1960622292659533
5	0.29448236794875576	1.2544678240669875
6	0.1449735051380404	0.741088570312755
7	0.079211564379382	0.4724075784236147
8	0.05830759720130964	0.39741600882769085
9	0.032422716489547844	0.24861202658391754
>10	0.11233334745179788	1.4808470174150032
>50	0.002302555907963425	0.13216486433072946
>100	7.557797487704321E-4	0.09971114123849324
>500	1.714380296398367E-5	0.010706348277314632
>1k	3.428695070280995E-5	0.03281951487057552
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.002168172204276E-6
2	0.0	1.800433634440855E-5	0.0	0.0	9.002168172204276E-6
3	0.0	1.800433634440855E-5	0.0	0.0	9.002168172204276E-6
4	0.0	2.7006504516612825E-5	0.0	9.002168172204276E-6	9.002168172204276E-6
5	0.0	4.501084086102138E-5	0.0	9.002168172204276E-6	9.002168172204276E-6
6	0.0	4.501084086102138E-5	0.0	9.002168172204276E-6	9.002168172204276E-6
7	0.0	4.501084086102138E-5	0.0	9.002168172204276E-6	1.800433634440855E-5
8	0.0	4.501084086102138E-5	0.0	9.002168172204276E-6	1.800433634440855E-5
9	0.0	8.101951354983848E-5	0.0	4.501084086102138E-5	1.800433634440855E-5
10-11	0.0	8.101951354983848E-5	0.0	5.851409311932779E-5	1.800433634440855E-5
12-13	0.0	8.101951354983848E-5	0.0	7.20173453776342E-5	3.1507588602714964E-5
14-15	0.0	1.2152927032475772E-4	0.0	8.101951354983848E-5	4.501084086102138E-5
16-17	0.0	1.2603035441085986E-4	0.0	9.902384989424702E-5	4.501084086102138E-5
18-19	0.0	1.4853577484137055E-4	0.0	1.3503252258306413E-4	5.401300903322565E-5
20-21	0.0	1.8004336344408552E-4	0.0	1.6654011118577908E-4	5.401300903322565E-5
22-23	0.0	2.2055312021900474E-4	0.0	2.025487838745962E-4	6.751626129153207E-5
24-25	0.0	2.385574565634133E-4	0.0	2.520607088217197E-4	8.101951354983848E-5
26-27	0.0	2.4305854064951543E-4	0.0	3.510845587159667E-4	8.101951354983848E-5
28-29	0.0	2.520607088217197E-4	0.0	6.931669492597292E-4	8.101951354983848E-5
30-31	0.0	2.880693815105368E-4	0.0	0.001993980250143247	8.552059763594062E-5
32-33	0.0	2.970715496827411E-4	0.0	0.0051672445308452546	9.902384989424702E-5
34-35	0.0	3.2857913828545607E-4	0.0	0.00960081235565586	1.1702818623865558E-4
36-37	0.0	3.5108455871596676E-4	0.0	0.015969846337490386	1.1702818623865558E-4
38-39	0.0	3.6008672688817104E-4	0.0	0.027447610757050836	1.1702818623865558E-4
40-41	0.0	4.050975677491924E-4	0.0	0.05815850747652572	1.1702818623865558E-4
42-43	0.0	4.095986518352945E-4	0.0	0.06874505724703794	1.1702818623865558E-4
44-45	0.0	4.5911057678241804E-4	0.0	0.08390020736494384	1.1702818623865558E-4
46-47	0.0	4.996203335573372E-4	0.0	0.10046419680179972	1.2603035441085986E-4
48-49	0.0	5.041214176434394E-4	0.0	0.11623599543950161	1.2603035441085986E-4
50-51	0.0	5.581344266766651E-4	0.0	0.13213832551570046	1.3503252258306413E-4
52-53	0.0	5.581344266766651E-4	0.0	0.15675475438259304	1.3503252258306413E-4
54-55	0.0	5.761387630210736E-4	0.0	0.21213609297799374	1.3503252258306413E-4
56-57	0.0	5.941430993654822E-4	0.0	0.27338684522167167	1.3953360666916627E-4
58-59	0.0	6.16648519795993E-4	0.0	0.3408986054291176	1.4853577484137055E-4
60-61	0.0	6.256506879681971E-4	0.0	0.36414670473383515	1.530368589274727E-4
62-63	0.0	6.391539402265036E-4	0.0	0.3756649789101705	1.530368589274727E-4
64-65	0.0	6.661604447431164E-4	0.0	0.38664312299617365	1.530368589274727E-4
66-67	0.0	6.751626129153207E-4	0.0	0.3932552155186577	1.530368589274727E-4
68-69	0.0	6.931669492597292E-4	0.0	0.39948021480973694	1.530368589274727E-4
70-71	0.0	7.111712856041378E-4	0.0	0.40537663496253074	1.530368589274727E-4
72-73	0.0	7.201734537763421E-4	0.0	0.4097471876101359	1.7104119527188124E-4
74-75	0.0	7.336767060346484E-4	0.0	0.41335705704718984	1.7104119527188124E-4
76-77	0.0	7.561821264651591E-4	0.0	0.4165933365050972	1.7104119527188124E-4
78-79	0.0	7.741864628095676E-4	0.0	0.4196450715154745	1.7104119527188124E-4
80-81	0.0	7.831886309817719E-4	0.0	0.42104940975033833	1.7554227935798338E-4
82-83	0.0	7.966918832400783E-4	0.0	0.4215805376724984	1.8904553161628977E-4
84-85	0.0	8.011929673261805E-4	0.0	0.4220261449970225	1.8904553161628977E-4
86-87	0.0	8.101951354983847E-4	0.0	0.4221836829400361	1.8904553161628977E-4
88-89	0.0	8.552059763594061E-4	0.0	0.4222466981172415	1.8904553161628977E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15375	0.0	50.0795	1
GTATCAA	27125	0.0	35.593975	1
TCAACGC	32990	0.0	29.657452	4
ATCAACG	33240	0.0	29.44844	3
TATCAAC	34305	0.0	28.672606	2
CAACGCA	34300	0.0	28.537344	5
AACGCAG	35595	0.0	27.557964	6
ACGCAGA	40900	0.0	24.004604	7
CGCAGAG	41480	0.0	23.762552	8
GTGGTAT	7385	0.0	23.026398	1
TGGTATC	7390	0.0	22.426786	2
GCAGAGT	48450	0.0	20.344082	9
GAGTACT	27970	0.0	19.840971	12-13
CAGAGTA	47705	0.0	18.699598	10-11
GTACTTT	30940	0.0	17.908792	14-15
AGAGTAC	44030	0.0	17.899277	10-11
TACATGG	33405	0.0	16.822083	2
GTACATG	33185	0.0	16.816551	1
ACATGGG	33940	0.0	15.921088	3
AGTACTT	30500	0.0	15.847386	12-13
>>END_MODULE