FastQCFastQC Report
Fri 27 May 2016
SRR2139704_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139704_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10831532
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA182250.054.297521
CTTATAC98300.035.535231
GTATCAA329400.035.1354561
ATCAACG410600.029.1486053
TCAACGC410300.029.147794
TATCAAC419950.028.5609362
CAACGCA419350.028.5187515
TATACAC144750.028.01573
AACGCAG434550.027.513926
TGGTATC74850.026.6020092
GTGGTAT77600.026.4334851
ACGCAGA491700.024.307277
CGCAGAG496600.024.1451518
TTATACA160800.022.8175132
ACATCTC170100.021.5304458
GCAGAGT562250.021.3487729
ACACATC172250.021.2617056
GAGTACT363800.020.5816912-13
TACACAT203950.019.9669935
CAGAGTA561150.019.72461310-11
Produced by FastQC (version 0.11.5)