##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139704_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10831532
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.88162800977738	31.0	18.0	33.0	18.0	33.0
2	30.031935740945972	32.0	28.0	33.0	25.0	33.0
3	31.564043018106766	33.0	32.0	33.0	27.0	34.0
4	31.747072897905856	33.0	32.0	33.0	30.0	34.0
5	32.150085971218104	33.0	33.0	34.0	31.0	34.0
6	35.63618895277233	38.0	36.0	38.0	30.0	38.0
7	35.99799557440259	38.0	37.0	38.0	31.0	38.0
8	36.3463244165276	38.0	37.0	38.0	33.0	38.0
9	36.473018405891246	38.0	38.0	38.0	34.0	38.0
10-11	36.578152241068025	38.0	38.0	38.0	34.0	38.0
12-13	36.596486304984374	38.0	38.0	38.0	34.0	38.0
14-15	36.619476496953524	38.0	38.0	38.0	34.0	38.0
16-17	36.62344781883117	38.0	38.0	38.0	34.0	38.0
18-19	36.638944010874916	38.0	38.0	38.0	34.0	38.0
20-21	36.63278862165362	38.0	38.0	38.0	34.0	38.0
22-23	36.629963364994154	38.0	38.0	38.0	34.0	38.0
24-25	36.62873939013127	38.0	38.0	38.0	34.0	38.0
26-27	36.57613296951425	38.0	38.0	38.0	34.0	38.0
28-29	36.55141582536814	38.0	38.0	38.0	34.0	38.0
30-31	36.55004210577356	38.0	38.0	38.0	34.0	38.0
32-33	36.5231175230711	38.0	38.0	38.0	34.0	38.0
34-35	36.47704474170315	38.0	38.0	38.0	34.0	38.0
36-37	36.453749897484556	38.0	38.0	38.0	34.0	38.0
38-39	36.41687883468982	38.0	38.0	38.0	34.0	38.0
40-41	36.44085795248541	38.0	38.0	38.0	34.0	38.0
42-43	36.3918099999332	38.0	38.0	38.0	34.0	38.0
44-45	36.33407640551617	38.0	38.0	38.0	33.0	38.0
46-47	36.28883965649011	38.0	38.0	38.0	33.0	38.0
48-49	36.236226094497226	38.0	38.0	38.0	33.0	38.0
50-51	36.21001035848899	38.0	37.0	38.0	33.0	38.0
52-53	36.19498823789105	38.0	37.5	38.0	33.0	38.0
54-55	36.16177585923734	38.0	37.0	38.0	33.0	38.0
56-57	36.1682783893302	38.0	37.0	38.0	33.0	38.0
58-59	36.14778781275429	38.0	37.0	38.0	33.0	38.0
60-61	36.13703473857753	38.0	37.0	38.0	33.0	38.0
62-63	36.1396032745956	38.0	37.0	38.0	33.0	38.0
64-65	36.132236897294746	38.0	37.0	38.0	33.0	38.0
66-67	36.13455419663977	38.0	37.0	38.0	33.0	38.0
68-69	36.11821998761407	38.0	37.0	38.0	33.0	38.0
70-71	36.092799049520764	38.0	37.0	38.0	33.0	38.0
72-73	36.06729353994488	38.0	37.0	38.0	32.5	38.0
74-75	36.08917514356108	38.0	37.0	38.0	33.0	38.0
76-77	36.07446067341628	38.0	37.0	38.0	33.0	38.0
78-79	36.06526159802729	38.0	37.0	38.0	33.0	38.0
80-81	36.02961781446426	38.0	37.0	38.0	31.5	38.0
82-83	36.03838605818575	38.0	37.0	38.0	32.0	38.0
84-85	36.036864695597	38.0	37.0	38.0	32.0	38.0
86-87	36.03547402865596	38.0	37.0	38.0	32.0	38.0
88-89	36.01327840420243	38.0	37.0	38.0	31.5	38.0
90-91	35.996327462621316	38.0	37.0	38.0	31.0	38.0
92-93	35.950342677580885	38.0	37.0	38.0	31.0	38.0
94-95	35.94580781285626	38.0	37.0	38.0	31.0	38.0
96-97	35.97801000485043	38.0	37.0	38.0	31.0	38.0
98-99	36.008807667864744	38.0	37.0	38.0	32.0	38.0
100-101	35.47745842371623	38.0	36.0	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	7.0
18	38.0
19	90.0
20	215.0
21	652.0
22	2762.0
23	8004.0
24	18493.0
25	35828.0
26	59763.0
27	91285.0
28	130329.0
29	174988.0
30	223495.0
31	280660.0
32	351074.0
33	449222.0
34	604002.0
35	901594.0
36	1815916.0
37	5683115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.51785989276494	24.00713952559989	14.83622076729312	23.638779814342055
2	14.72669794078991	21.733112176560066	41.77071165925559	21.769478223394437
3	17.628329679531834	27.63035623525958	30.89422810154198	23.84708598366661
4	12.62243420413659	19.478685009655145	39.26876641272906	28.63011437347921
5	12.935750817113606	37.79219709157067	36.27026526725878	13.00178682405695
6	28.12998198223483	39.618596889156585	18.931984875269723	13.319436253338862
7	24.979827414995405	33.88317552863251	23.490425915743035	17.646571140629046
8	23.237894694859413	38.066868103237844	21.389088819568645	17.306148382334097
9	24.596631390647232	17.60503500335871	21.842007206367484	35.95632639962657
10-11	23.240558214664368	27.819088749403132	29.267941967950613	19.672411067981887
12-13	24.387565858643082	25.19957010698025	29.53085491507573	20.882009119300946
14-15	21.51170305364006	26.659986786726016	27.34275723877287	24.485552920861057
16-17	20.657197892227988	29.95480233082449	28.054470964956757	21.333528811990767
18-19	20.632030630570082	28.609457092496243	30.304360454273688	20.454151822659988
20-21	21.46792255253531	27.59289126571762	30.3889748901303	20.550211291616776
22-23	21.167187378380184	27.676397729384846	30.273243690958267	20.883171201276706
24-25	20.974382062979206	27.930583886876253	30.37666929188138	20.718364758263156
26-27	20.984244263014766	28.032882156493706	30.306317063805917	20.67655651668561
28-29	20.758685756634332	27.882295268598213	30.513244635335905	20.845774339431554
30-31	21.265274034027676	27.841291442267146	30.26098807004054	20.632446453664638
32-33	21.016477372771387	27.827210684015064	30.27173753594841	20.884574407265134
34-35	20.88469742606638	28.105529902686	30.46822394601904	20.54154872522858
36-37	21.05302452433304	27.906768608782777	30.268714449478836	20.771492417405344
38-39	20.797838158712125	27.920792060620318	30.370144497596335	20.91122528307122
40-41	21.036183128963966	27.8221094577302	30.308073314472345	20.833634098833496
42-43	21.162038385014224	27.831798679141244	30.042169658563022	20.96399327728151
44-45	20.989139538207773	28.115637021418994	29.870151353514473	21.025072086858763
46-47	21.300521812126338	27.934014666031914	29.462405539769566	21.303057982072183
48-49	21.141915257579296	28.09490319618248	29.41068039489043	21.352501151347784
50-51	21.012799828770234	28.219722144640986	29.451682085701464	21.315795940887316
52-53	21.216183303506067	28.15520109982421	29.494713666181276	21.13390193048844
54-55	21.1415657736563	28.153058549553595	29.330700462793963	21.37467521399615
56-57	21.375864631889623	28.351049947414168	28.991641408701412	21.28144401199479
58-59	21.050628864914042	28.659559607250497	29.3154382871041	20.97437324073136
60-61	21.314169801052135	28.85447900300089	28.939210967416745	20.892140228530227
62-63	21.051844701631794	29.065346417133174	28.91771114563068	20.965097735604346
64-65	21.006970135632788	29.003974636494345	28.9373622191889	21.051693008683962
66-67	21.149623098653915	28.975069751000852	28.78848075479067	21.08682639555456
68-69	21.124379491816825	28.78696830443428	28.94575258311334	21.142899620635554
70-71	21.191170622161398	28.358352181231204	29.076478777142174	21.373998419465224
72-73	21.241203020723376	28.361677990330175	29.06133996364037	21.33577902530608
74-75	21.173584531813844	28.42280965240959	29.012986878756404	21.390618937020164
76-77	21.26095463287514	28.35796373849309	28.958967826446536	21.422113802185237
78-79	21.25562270109124	28.410944584625103	28.926035844542476	21.40739686974118
80-81	21.315068173283365	28.408321281283893	28.87104290098866	21.405567644444083
82-83	21.36357975046895	28.398637134085845	28.954289382824918	21.283493732620293
84-85	21.486439996163185	28.379679528550856	28.81049361272476	21.323386862561208
86-87	21.2772831882325	28.45359093850072	28.88487445199621	21.384251421270562
88-89	21.371213786082297	28.454191569920233	28.78199612708623	21.39259851691124
90-91	21.419663111818377	28.425035332532772	28.856554555136256	21.298747000512595
92-93	21.471791484920345	28.40542980965939	28.78628546536588	21.336493240054384
94-95	21.304928674385017	28.553277095430357	28.73799794185189	21.40379628833273
96-97	21.32165653976126	28.56036504334181	28.811254044827823	21.306724372069105
98-99	21.543052670107198	29.09779997961579	28.83463213231019	20.524515217966822
100-101	22.02197009869268	31.666369453286514	26.34600018938879	19.965660258632013
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3316.0
1	3194.5
2	4070.5
3	5292.0
4	6446.5
5	8942.0
6	11423.5
7	15156.5
8	19346.0
9	21147.5
10	20185.0
11	18763.0
12	19933.0
13	24612.5
14	35629.0
15	49411.5
16	59432.5
17	64530.0
18	65302.5
19	62127.0
20	56895.0
21	52394.0
22	51851.5
23	57666.0
24	69746.5
25	88464.5
26	112822.0
27	139035.5
28	165528.0
29	192990.5
30	218715.5
31	244271.5
32	272742.0
33	302697.0
34	330750.5
35	355521.0
36	382919.5
37	406124.5
38	419462.0
39	429478.0
40	439342.5
41	446817.5
42	448631.5
43	454321.0
44	475440.5
45	484161.5
46	466405.5
47	448263.5
48	431352.5
49	410404.0
50	390375.0
51	368371.0
52	342641.0
53	317372.5
54	291682.5
55	264528.5
56	235997.5
57	207545.0
58	181834.5
59	156138.5
60	129158.0
61	103767.0
62	80289.0
63	60431.5
64	45574.5
65	33729.5
66	24251.0
67	18204.0
68	13935.0
69	10106.0
70	7199.0
71	5159.0
72	3678.5
73	2542.0
74	1754.5
75	1240.5
76	810.0
77	487.5
78	330.0
79	212.5
80	125.0
81	75.5
82	60.0
83	50.5
84	35.5
85	24.0
86	14.5
87	7.5
88	4.5
89	3.5
90	2.5
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0032036096094255184
4	0.0
5	0.05188555044660349
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	7.435250856820298E-4
28-29	1.8943313336124932E-4
30-31	0.0
32-33	0.0
34-35	3.718105164373711E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	9.589683801753425E-6
46-47	1.11320312631966E-4
48-49	0.0
50-51	2.462461498183245E-5
52-53	4.962388814577117E-6
54-55	9.899665886276337E-4
56-57	0.0020664951778083013
58-59	0.008960579852692744
60-61	0.007786654883306545
62-63	0.00398048301972469
64-65	0.005088877559416035
66-67	0.0066036452121571105
68-69	0.003578229222748443
70-71	0.005339286610965677
72-73	9.478210357003875E-4
74-75	0.0015943200862560151
76-77	0.0013432063067199392
78-79	0.001031741933081779
80-81	4.534039585566112E-5
82-83	0.0
84-85	0.0
86-87	3.27601937437858E-4
88-89	9.228180425834386E-4
90-91	0.0
92-93	1.2113728039779544E-5
94-95	1.3486387484142E-4
96-97	3.293392764366385E-4
98-99	1.2820256415384511E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	165.0
20-21	700.0
22-23	1375.0
24-25	1784.0
26-27	3934.0
28-29	8577.0
30-31	20851.0
32-33	27596.0
34-35	37788.0
36-37	57915.0
38-39	69737.0
40-41	69301.0
42-43	80711.0
44-45	95778.0
46-47	95923.0
48-49	87122.0
50-51	78246.0
52-53	74238.0
54-55	78969.0
56-57	82378.0
58-59	83521.0
60-61	82546.0
62-63	81138.0
64-65	93265.0
66-67	103268.0
68-69	101292.0
70-71	113030.0
72-73	84699.0
74-75	85307.0
76-77	90065.0
78-79	93999.0
80-81	95694.0
82-83	90987.0
84-85	89795.0
86-87	93379.0
88-89	97134.0
90-91	99856.0
92-93	98186.0
94-95	103672.0
96-97	162477.0
98-99	865885.0
100-101	7049249.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.44072696646685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.48171816011423	75.55901331648545
2	7.280327939686211	12.295123675627668
3	1.7976253039829189	4.553783624449009
4	0.6755747155169823	2.281840803936721
5	0.2937692667405668	1.2403045221989686
6	0.15693200672044288	0.7950871639068395
7	0.08596963770591536	0.5081537093451891
8	0.05595882551106468	0.3780163125075178
9	0.036515137168297976	0.2775028254994201
>10	0.13100363529971157	1.7069621581068148
>50	0.003667549652573812	0.21503228964964743
>100	8.469970186432633E-4	0.12613862649181246
>500	5.4496669139879744E-5	0.028331295795539276
>1k	3.632821322182522E-5	0.034709675999367806
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.8464608699858894E-5	0.0	0.0	9.232304349929447E-6
3	0.0	2.769691304978834E-5	0.0	0.0	9.232304349929447E-6
4	0.0	6.462613044950612E-5	0.0	2.769691304978834E-5	9.232304349929447E-6
5	0.0	6.462613044950612E-5	0.0	2.769691304978834E-5	9.232304349929447E-6
6	0.0	6.462613044950612E-5	0.0	2.769691304978834E-5	9.232304349929447E-6
7	0.0	6.462613044950612E-5	0.0	2.769691304978834E-5	9.232304349929447E-6
8	0.0	8.309073914936502E-5	0.0	3.692921739971779E-5	9.232304349929447E-6
9	0.0	1.754137826486595E-4	0.0	5.539382609957668E-5	9.232304349929447E-6
10-11	0.0	1.754137826486595E-4	0.0	6.462613044950612E-5	9.232304349929447E-6
12-13	0.0	1.754137826486595E-4	0.0	6.924228262447085E-5	9.232304349929447E-6
14-15	0.0	2.446560652731303E-4	0.0	1.0617150002418864E-4	1.8464608699858894E-5
16-17	4.616152174964723E-6	2.4927221744809505E-4	0.0	1.2925226089901225E-4	2.769691304978834E-5
18-19	9.232304349929447E-6	2.585045217980245E-4	0.0	1.384845652489417E-4	2.769691304978834E-5
20-21	9.232304349929447E-6	2.6773682614795395E-4	0.0	1.6156532612376532E-4	2.769691304978834E-5
22-23	9.232304349929447E-6	3.369791087724248E-4	0.0	1.8926223917355365E-4	3.231306522475306E-5
24-25	9.232304349929447E-6	4.200698479217899E-4	0.0	2.16959152223342E-4	4.616152174964723E-5
26-27	9.232304349929447E-6	4.8007982619633125E-4	0.0	2.5388836962305977E-4	4.616152174964723E-5
28-29	9.232304349929447E-6	4.846959783712959E-4	0.0	4.385344566216487E-4	5.0777673924611956E-5
30-31	9.232304349929447E-6	5.354736522959079E-4	0.0	0.0011217249785164278	5.539382609957668E-5
32-33	9.232304349929447E-6	5.354736522959079E-4	0.0	0.0024604091092561976	5.539382609957668E-5
34-35	9.232304349929447E-6	6.370290001451318E-4	0.0	0.004634616783664582	5.539382609957668E-5
36-37	9.232304349929447E-6	7.293520436444262E-4	0.0	0.007565873414767181	5.539382609957668E-5
38-39	9.232304349929447E-6	7.893620219189677E-4	0.0	0.012209722502781694	5.539382609957668E-5
40-41	9.232304349929447E-6	8.770689132432974E-4	0.0	0.017079763047369478	5.539382609957668E-5
42-43	9.232304349929447E-6	8.955335219431563E-4	0.0	0.022005197418056836	5.539382609957668E-5
44-45	9.232304349929447E-6	9.555435002176977E-4	0.0	0.027087580962692995	5.539382609957668E-5
46-47	9.232304349929447E-6	9.878565654424507E-4	0.0	0.03250694361610158	6.00099782745414E-5
48-49	9.232304349929447E-6	9.924727176174155E-4	0.0	0.038069406986934076	8.309073914936502E-5
50-51	9.232304349929447E-6	0.0010940280654666394	0.0	0.04399193022741381	8.309073914936502E-5
52-53	9.232304349929447E-6	0.0010986442176416041	0.0	0.05001600881574278	8.309073914936502E-5
54-55	9.232304349929447E-6	0.0010986442176416041	0.0	0.05577235057792379	8.309073914936502E-5
56-57	9.232304349929447E-6	0.0010986442176416041	0.0	0.06179181301407779	8.309073914936502E-5
58-59	9.232304349929447E-6	0.0011032603698165689	0.0	0.06786205312415639	8.309073914936502E-5
60-61	1.8464608699858894E-5	0.0011494218915662161	0.0	0.0735537687558879	8.309073914936502E-5
62-63	1.8464608699858894E-5	0.0011955834133158634	0.0	0.07909776751802053	8.309073914936502E-5
64-65	1.8464608699858894E-5	0.0013479164350896993	0.0	0.08489103849760127	8.309073914936502E-5
66-67	1.8464608699858894E-5	0.0013571487394396287	0.0	0.09045350186843376	9.232304349929447E-5
68-69	1.8464608699858894E-5	0.001384845652489417	0.0	0.09622369208713966	1.0155534784922391E-4
70-71	1.8464608699858894E-5	0.0014310071742390643	0.0	0.10183693313189676	1.0155534784922391E-4
72-73	1.8464608699858894E-5	0.0014402394785889938	0.0	0.10594992471979034	1.2925226089901225E-4
74-75	1.8464608699858894E-5	0.001481784848163676	0.0	0.1092920188944648	1.2925226089901225E-4
76-77	1.8464608699858894E-5	0.0015371786742632528	0.0	0.11231098241689172	1.2925226089901225E-4
78-79	1.8464608699858894E-5	0.0015694917394880059	0.0	0.11486833072182218	1.3386841307397697E-4
80-81	1.8464608699858894E-5	0.0015741078916629706	0.0	0.11614238872211244	1.5233302177383587E-4
82-83	1.8464608699858894E-5	0.0015787240438379353	0.0	0.11666401391788345	1.6618147829873005E-4
84-85	1.8464608699858894E-5	0.0015787240438379353	0.0	0.11726411370062886	1.6618147829873005E-4
86-87	1.8464608699858894E-5	0.001601804804712759	0.0	0.11751338591807697	1.6618147829873005E-4
88-89	1.8464608699858894E-5	0.0016941278482120535	0.0	0.11758262820070144	1.6618147829873005E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18225	0.0	54.29752	1
CTTATAC	9830	0.0	35.53523	1
GTATCAA	32940	0.0	35.135456	1
ATCAACG	41060	0.0	29.148605	3
TCAACGC	41030	0.0	29.14779	4
TATCAAC	41995	0.0	28.560936	2
CAACGCA	41935	0.0	28.518751	5
TATACAC	14475	0.0	28.0157	3
AACGCAG	43455	0.0	27.51392	6
TGGTATC	7485	0.0	26.602009	2
GTGGTAT	7760	0.0	26.433485	1
ACGCAGA	49170	0.0	24.30727	7
CGCAGAG	49660	0.0	24.145151	8
TTATACA	16080	0.0	22.817513	2
ACATCTC	17010	0.0	21.530445	8
GCAGAGT	56225	0.0	21.348772	9
ACACATC	17225	0.0	21.261705	6
GAGTACT	36380	0.0	20.58169	12-13
TACACAT	20395	0.0	19.966993	5
CAGAGTA	56115	0.0	19.724613	10-11
>>END_MODULE