FastQCFastQC Report
Fri 27 May 2016
SRR2139703_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139703_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9295452
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127000.050.4307331
GTATCAA233050.036.36521
CTTATAC77450.033.133561
TCAACGC283900.030.1696324
ATCAACG286100.029.8784683
CAACGCA292850.029.1739625
TATCAAC296850.029.087042
AACGCAG304500.028.1142256
TATACAC117750.024.9069483
GTGGTAT62750.024.3311181
ACGCAGA354250.024.1902687
TGGTATC60400.023.9210592
CGCAGAG359000.023.8581948
TTATACA123300.022.211772
GCAGAGT417300.020.5353539
ACATCTC134750.020.3452288
ACACATC131550.019.9554086
GAGTACT249750.019.80529812-13
TATGCCG76650.019.67306742-43
CTCGTAT76850.018.88317338-39
Produced by FastQC (version 0.11.5)