##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139703_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9295452
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.063533220331834	32.0	18.0	33.0	18.0	33.0
2	30.05755933116539	32.0	28.0	33.0	25.0	33.0
3	31.566794062300573	33.0	32.0	33.0	27.0	34.0
4	31.739275292906683	33.0	32.0	33.0	30.0	34.0
5	32.127271272015605	33.0	33.0	34.0	31.0	34.0
6	35.60717574573028	38.0	36.0	38.0	30.0	38.0
7	35.978478722713	38.0	37.0	38.0	31.0	38.0
8	36.323054220494065	38.0	37.0	38.0	33.0	38.0
9	36.45904728462909	38.0	37.0	38.0	34.0	38.0
10-11	36.567376497667894	38.0	38.0	38.0	34.0	38.0
12-13	36.5883678921692	38.0	38.0	38.0	34.0	38.0
14-15	36.6088915310412	38.0	38.0	38.0	34.0	38.0
16-17	36.612486514910735	38.0	38.0	38.0	34.0	38.0
18-19	36.621338908532906	38.0	38.0	38.0	34.0	38.0
20-21	36.61453664506068	38.0	38.0	38.0	34.0	38.0
22-23	36.61175966104763	38.0	38.0	38.0	34.0	38.0
24-25	36.61003123089023	38.0	38.0	38.0	34.0	38.0
26-27	36.556389456792786	38.0	38.0	38.0	34.0	38.0
28-29	36.53036494513193	38.0	38.0	38.0	34.0	38.0
30-31	36.52861461759673	38.0	38.0	38.0	34.0	38.0
32-33	36.50856793159933	38.0	38.0	38.0	34.0	38.0
34-35	36.475433678426825	38.0	38.0	38.0	34.0	38.0
36-37	36.455802134688874	38.0	38.0	38.0	34.0	38.0
38-39	36.4325475611694	38.0	38.0	38.0	34.0	38.0
40-41	36.44247658003138	38.0	38.0	38.0	34.0	38.0
42-43	36.39263200563825	38.0	38.0	38.0	34.0	38.0
44-45	36.34303404671205	38.0	38.0	38.0	33.0	38.0
46-47	36.30249308865174	38.0	38.0	38.0	33.0	38.0
48-49	36.257672272801656	38.0	38.0	38.0	33.0	38.0
50-51	36.22781718398592	38.0	37.0	38.0	33.0	38.0
52-53	36.20967283895136	38.0	37.0	38.0	33.0	38.0
54-55	36.1751443635305	38.0	37.0	38.0	33.0	38.0
56-57	36.17475517331846	38.0	37.0	38.0	33.0	38.0
58-59	36.15489245423417	38.0	37.0	38.0	33.0	38.0
60-61	36.14170436768699	38.0	37.0	38.0	33.0	38.0
62-63	36.1408775623329	38.0	37.0	38.0	33.0	38.0
64-65	36.13072928702077	38.0	37.0	38.0	33.0	38.0
66-67	36.13011882381742	38.0	37.0	38.0	33.0	38.0
68-69	36.11691213645659	38.0	37.0	38.0	33.0	38.0
70-71	36.08683981465118	38.0	37.0	38.0	33.0	38.0
72-73	36.058269958093646	38.0	37.0	38.0	32.5	38.0
74-75	36.0777388969057	38.0	37.0	38.0	33.0	38.0
76-77	36.06281175516369	38.0	37.0	38.0	32.5	38.0
78-79	36.04982998102493	38.0	37.0	38.0	32.0	38.0
80-81	36.008543243318954	38.0	37.0	38.0	31.0	38.0
82-83	36.01072870758832	38.0	37.0	38.0	31.0	38.0
84-85	36.003349441760584	38.0	37.0	38.0	31.0	38.0
86-87	36.0049777428803	38.0	37.0	38.0	31.0	38.0
88-89	35.976516150806006	38.0	37.0	38.0	31.0	38.0
90-91	35.958277387475746	38.0	37.0	38.0	31.0	38.0
92-93	35.910567852896136	38.0	37.0	38.0	31.0	38.0
94-95	35.90489107404737	38.0	37.0	38.0	31.0	38.0
96-97	35.936476551954165	38.0	37.0	38.0	31.0	38.0
98-99	35.97099496032966	38.0	37.0	38.0	31.0	38.0
100-101	35.44842017462331	38.0	36.0	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	28.0
19	55.0
20	124.0
21	566.0
22	2232.0
23	6753.0
24	15935.0
25	30853.0
26	50848.0
27	77950.0
28	110634.0
29	148939.0
30	191702.0
31	241561.0
32	304646.0
33	389137.0
34	523412.0
35	780888.0
36	1556442.0
37	4862744.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.03369647866505	23.208909044982427	14.49714333418106	24.260251142171462
2	15.21169707508575	21.239795547327876	40.92081805166656	22.627689325919814
3	18.140892679631122	26.68784614788772	30.14677531102208	25.024485861459084
4	13.157122429334258	18.86029856321134	38.290262808091526	29.69231619936287
5	13.73508808809035	37.11016557196311	35.65714450549871	13.497601834447842
6	29.461504400216366	38.511091230421066	18.484383545845862	13.543020823516706
7	26.114308373600338	33.00081588286401	22.874336826224265	18.010538917311393
8	23.88658453617963	37.48223324696852	20.895713301515624	17.73546891533623
9	24.902177968322572	17.46238913395497	21.284978933783965	36.35045396393849
10-11	23.887504340832486	27.21143092342363	28.74302938684423	20.158035348899656
12-13	24.890709994522055	24.535536303129746	28.95265878410216	21.621094918246044
14-15	22.016791977968147	25.99838490883255	26.579905944311143	25.404917168888158
16-17	21.479245979646823	29.216233917403912	27.31317960654307	21.9913404964062
18-19	21.49014916111664	28.106250239364368	29.255317546688424	21.148283052830568
20-21	22.239549473069523	27.122710752557573	29.281829889656535	21.355909884716375
22-23	21.974553301880093	27.22402816073429	29.132024852162168	21.669393685223447
24-25	21.851062582079024	27.373863940212846	29.210976114297583	21.56409736341055
26-27	21.83844872393744	27.476903485300134	29.12964057786877	21.55500721289366
28-29	21.646421290438717	27.356359174090283	29.23785600410664	21.759363531364365
30-31	21.929101672455904	27.29954065441228	29.22490389348838	21.546453779643436
32-33	21.59165458466192	27.293102956819627	29.336716294661848	21.7785261638566
34-35	21.685801844006615	27.369151666137064	29.184001823753512	21.761044666102805
36-37	21.709892365006997	27.345423614161575	29.289474539104127	21.6552094817273
38-39	21.59422288653031	27.412476605306775	29.308172527959613	21.685127980203305
40-41	21.880163655438185	27.268584845798333	29.21679740531406	21.634454093449424
42-43	21.888307029680902	27.345950659688338	29.023289905945386	21.742452404685373
44-45	21.85959879001933	27.503295136122823	28.83438528859599	21.802720785261855
46-47	22.042983715701425	27.40653377875908	28.480797967995535	22.069684537543957
48-49	21.927338024322278	27.524725153303315	28.47505839181959	22.072878430554816
50-51	21.81487731933838	27.64265557741585	28.48398266489891	22.05848443834686
52-53	22.011127953871465	27.59568196559039	28.53878836333572	21.854401717202425
54-55	21.96017755651624	27.52368109470712	28.420319723594005	22.095821625182637
56-57	22.100882432636105	27.762281961667583	28.176670515715312	21.960165089980997
58-59	21.861908158507692	27.953832507638804	28.40807385603003	21.77618547782347
60-61	22.04807892079313	28.09397319116415	28.155773928311618	21.7021739597311
62-63	21.8824496919299	28.290033923830933	28.06886642928118	21.758649954957992
64-65	21.827293548415494	28.23060672467989	28.08596155634101	21.856138170563604
66-67	21.93404744043593	28.2008442354136	27.951741352697425	21.91336697145304
68-69	21.90525058170693	28.061916106692863	28.09975695172596	21.933076359874242
70-71	21.975749689904145	27.69851759327083	28.21080502114767	22.114927695677352
72-73	22.069998384067382	27.64820531660665	28.16239708474204	22.11939921458392
74-75	21.913136304888287	27.759518765968892	28.169700556146925	22.157644372995893
76-77	22.045068432093196	27.71628556378297	28.087369235219445	22.15127676890439
78-79	22.028897155635303	27.71378008657517	28.080414112063686	22.17690864572584
80-81	22.056564266753178	27.744722249677338	28.04943929403806	22.149274189531422
82-83	22.10772719578518	27.744617032249398	28.098508754423644	22.049147017541777
84-85	22.190073315825302	27.72979424714687	28.01681297859459	22.063319458433238
86-87	22.052074059098306	27.743055422576564	28.07699339320042	22.127877125124712
88-89	22.105843615865712	27.74985628393202	27.993723690961385	22.150576409240884
90-91	22.171395334384236	27.76687921664956	28.023436236550243	22.03828921241596
92-93	22.172165365214624	27.75946498999079	27.977479623859292	22.090890020935294
94-95	22.05530306975277	27.808599153202184	27.98957795950384	22.146519817541204
96-97	22.105329356508467	27.796755171864156	28.012905640908325	22.08500983071905
98-99	22.354839425923213	28.37228500095505	28.05839787209921	21.214477701022528
100-101	22.878221513518866	30.906355373422294	25.47185583949379	20.74356727356505
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2008.0
1	1958.5
2	2555.5
3	3347.5
4	4146.5
5	5864.0
6	7601.0
7	9919.0
8	12357.0
9	13569.5
10	13347.5
11	12686.5
12	13606.5
13	16934.0
14	24291.5
15	33666.0
16	40833.0
17	44445.5
18	44469.5
19	41850.0
20	38245.0
21	35234.5
22	34771.5
23	38544.0
24	47336.5
25	61140.5
26	78820.5
27	97692.5
28	117069.0
29	137947.5
30	157956.5
31	178000.5
32	200553.5
33	223551.0
34	244834.0
35	265673.0
36	289099.5
37	308576.0
38	321441.0
39	332626.0
40	344675.0
41	357038.5
42	364632.5
43	371095.0
44	380290.0
45	394971.0
46	402854.0
47	397843.5
48	390775.0
49	379754.5
50	367983.0
51	354600.5
52	336524.5
53	318008.0
54	298833.5
55	276421.0
56	251479.0
57	224922.0
58	199255.5
59	172764.5
60	144177.5
61	117437.5
62	92478.5
63	70813.0
64	53276.5
65	39051.0
66	28339.5
67	21364.0
68	16211.0
69	11719.0
70	8417.5
71	6015.0
72	4217.0
73	2825.5
74	1857.0
75	1240.0
76	782.0
77	456.0
78	282.0
79	197.5
80	133.5
81	76.0
82	43.0
83	28.5
84	22.0
85	19.0
86	14.5
87	10.5
88	8.5
89	7.5
90	5.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.003141321153613617
4	0.0
5	0.05173497749221878
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.6136923734316526E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.555636388682023E-4
28-29	2.1532243026675435E-4
30-31	0.0
32-33	0.0
34-35	6.494844473642378E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	5.570813084124124E-6
46-47	1.3485245371624988E-4
48-49	0.0
50-51	1.7130897818363034E-5
52-53	2.875498891868992E-5
54-55	0.0011236454859106441
56-57	0.0021305320358762916
58-59	0.008179088377638984
60-61	0.007433096642892029
62-63	0.0039706193305057425
64-65	0.004925772467536477
66-67	0.006201321678743421
68-69	0.003504228404869697
70-71	0.005263801273665898
72-73	0.0010672323751595512
74-75	0.001513586401179812
76-77	0.0013037801315411894
78-79	0.0011035879256896812
80-81	5.2157453965179164E-5
82-83	0.0
84-85	0.0
86-87	4.367291059389246E-4
88-89	9.099954099559881E-4
90-91	0.0
92-93	6.946016463031459E-6
94-95	1.4757695505733577E-4
96-97	3.204962250174083E-4
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	117.0
20-21	452.0
22-23	1024.0
24-25	1249.0
26-27	2838.0
28-29	6545.0
30-31	16530.0
32-33	21991.0
34-35	29884.0
36-37	46851.0
38-39	55579.0
40-41	54944.0
42-43	63651.0
44-45	75772.0
46-47	76060.0
48-49	69778.0
50-51	63094.0
52-53	60451.0
54-55	65735.0
56-57	68332.0
58-59	68785.0
60-61	67750.0
62-63	66910.0
64-65	76238.0
66-67	82034.0
68-69	81538.0
70-71	89322.0
72-73	69475.0
74-75	70743.0
76-77	74938.0
78-79	77960.0
80-81	78640.0
82-83	74878.0
84-85	74422.0
86-87	78105.0
88-89	81084.0
90-91	82967.0
92-93	82712.0
94-95	88812.0
96-97	142087.0
98-99	772168.0
100-101	6133007.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.54064181539468
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.03048083522899	77.01265113594079
2	7.039295961166711	12.04291788893432
3	1.6326505321049072	4.189739231294981
4	0.5837840522631251	1.997490500487185
5	0.2837699307045238	1.2136931000187519
6	0.1421097244295949	0.7293694221549853
7	0.08616337816614267	0.5159329468520085
8	0.05151968522286985	0.3525621552073113
9	0.03346734259010486	0.2576536168511937
>10	0.11426122747763136	1.490973669073677
>50	0.00207594612276766	0.12183457971589522
>100	3.808134219438116E-4	0.05028107395857817
>500	4.057110061891388E-5	0.02490067951033917
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.0757949156211015E-5	0.0	1.0757949156211015E-5	0.0
3	0.0	1.0757949156211015E-5	0.0	1.0757949156211015E-5	0.0
4	0.0	6.454769493726609E-5	0.0	1.0757949156211015E-5	0.0
5	0.0	6.454769493726609E-5	0.0	1.0757949156211015E-5	0.0
6	1.0757949156211015E-5	6.454769493726609E-5	0.0	1.0757949156211015E-5	0.0
7	1.0757949156211015E-5	6.454769493726609E-5	0.0	1.0757949156211015E-5	0.0
8	1.0757949156211015E-5	8.606359324968812E-5	0.0	1.0757949156211015E-5	0.0
9	1.0757949156211015E-5	1.0757949156211016E-4	0.0	5.378974578105508E-5	0.0
10-11	1.0757949156211015E-5	1.0757949156211016E-4	0.0	7.53056440934771E-5	0.0
12-13	1.0757949156211015E-5	1.2909538987453218E-4	0.0	7.53056440934771E-5	0.0
14-15	1.0757949156211015E-5	1.9902205938990378E-4	0.0	7.53056440934771E-5	0.0
16-17	1.0757949156211015E-5	2.044010339680093E-4	0.0	7.53056440934771E-5	1.6136923734316522E-5
18-19	1.0757949156211015E-5	2.689487289052754E-4	0.0	7.53056440934771E-5	2.151589831242203E-5
20-21	1.0757949156211015E-5	3.334964238425415E-4	0.0	8.606359324968812E-5	2.151589831242203E-5
22-23	1.0757949156211015E-5	3.7114924588928005E-4	0.0	9.682154240589914E-5	2.151589831242203E-5
24-25	1.0757949156211015E-5	4.4107591540465163E-4	0.0	1.1833744071832117E-4	2.151589831242203E-5
26-27	1.0757949156211015E-5	4.841077120294957E-4	0.0	1.7212718649937627E-4	3.2273847468633044E-5
28-29	1.0757949156211015E-5	4.841077120294957E-4	0.0	3.442543729987525E-4	3.2273847468633044E-5
30-31	1.0757949156211015E-5	5.594133561229728E-4	0.0	9.090467036998308E-4	3.2273847468633044E-5
32-33	1.0757949156211015E-5	5.594133561229728E-4	0.0	0.0022107585516013637	3.2273847468633044E-5
34-35	1.0757949156211015E-5	6.185820764821335E-4	0.0	0.004152568374297452	3.2273847468633044E-5
36-37	1.0757949156211015E-5	6.77750796841294E-4	0.0	0.0071163833668335865	3.2273847468633044E-5
38-39	1.0757949156211015E-5	7.207825934661381E-4	0.0	0.011360394308958834	3.765282204673855E-5
40-41	1.0757949156211015E-5	7.960882375596151E-4	0.0	0.01605623911564494	4.303179662484406E-5
42-43	1.0757949156211015E-5	8.283620850282481E-4	0.0	0.020337902879816926	4.303179662484406E-5
44-45	1.0757949156211015E-5	9.251836274341474E-4	0.0	0.02541027590697042	4.841077120294957E-5
46-47	1.0757949156211015E-5	9.897313223714134E-4	0.0	0.031020546391934467	5.378974578105508E-5
48-49	1.0757949156211015E-5	0.00100586824610573	0.0	0.03674915431761683	5.378974578105508E-5
50-51	4.303179662484406E-5	0.0010596579918867853	0.0	0.04202054940416023	5.378974578105508E-5
52-53	4.303179662484406E-5	0.0010757949156211017	0.0	0.0476577147620148	5.378974578105508E-5
54-55	4.303179662484406E-5	0.0010757949156211017	0.0	0.053397080636853375	5.378974578105508E-5
56-57	4.303179662484406E-5	0.0010865528647773127	0.0	0.058905150604833416	5.378974578105508E-5
58-59	4.303179662484406E-5	0.0010973108139335237	0.0	0.06440784159823534	5.378974578105508E-5
60-61	4.303179662484406E-5	0.0010973108139335237	0.0	0.06980295310007517	5.378974578105508E-5
62-63	4.303179662484406E-5	0.0010973108139335237	0.0	0.07497752664421267	6.454769493726609E-5
64-65	4.303179662484406E-5	0.001237164152964267	0.0	0.0799853519764289	6.454769493726609E-5
66-67	5.378974578105508E-5	0.0012694380004329	0.0	0.08510613577478535	6.454769493726609E-5
68-69	5.378974578105508E-5	0.001301711847901533	0.0	0.09038290983590686	6.454769493726609E-5
70-71	5.378974578105508E-5	0.0013178487716358493	0.0	0.09517557618499886	6.454769493726609E-5
72-73	5.378974578105508E-5	0.0013286067207920606	0.0	0.09900002711003188	6.454769493726609E-5
74-75	5.378974578105508E-5	0.00137701749199501	0.0	0.10231885442472297	6.454769493726609E-5
76-77	5.378974578105508E-5	0.0014469441615103816	0.0	0.10512667915449406	6.454769493726609E-5
78-79	5.378974578105508E-5	0.0014845969835571202	0.0	0.10770858695198468	6.454769493726609E-5
80-81	5.378974578105508E-5	0.0014845969835571202	0.0	0.10890271930832413	6.454769493726609E-5
82-83	5.378974578105508E-5	0.0014953549327133312	0.0	0.10926848957963528	7.53056440934771E-5
84-85	5.378974578105508E-5	0.0015007339072914366	0.0	0.10961274395263404	7.53056440934771E-5
86-87	5.378974578105508E-5	0.0015061128818695422	0.0	0.10978487113913342	7.53056440934771E-5
88-89	5.378974578105508E-5	0.0016029344242754415	0.0	0.10982790293575827	7.53056440934771E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12700	0.0	50.430733	1
GTATCAA	23305	0.0	36.3652	1
CTTATAC	7745	0.0	33.13356	1
TCAACGC	28390	0.0	30.169632	4
ATCAACG	28610	0.0	29.878468	3
CAACGCA	29285	0.0	29.173962	5
TATCAAC	29685	0.0	29.08704	2
AACGCAG	30450	0.0	28.114225	6
TATACAC	11775	0.0	24.906948	3
GTGGTAT	6275	0.0	24.331118	1
ACGCAGA	35425	0.0	24.190268	7
TGGTATC	6040	0.0	23.921059	2
CGCAGAG	35900	0.0	23.858194	8
TTATACA	12330	0.0	22.21177	2
GCAGAGT	41730	0.0	20.535353	9
ACATCTC	13475	0.0	20.345228	8
ACACATC	13155	0.0	19.955408	6
GAGTACT	24975	0.0	19.805298	12-13
TATGCCG	7665	0.0	19.673067	42-43
CTCGTAT	7685	0.0	18.883173	38-39
>>END_MODULE