FastQCFastQC Report
Fri 27 May 2016
SRR2139702_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139702_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15480598
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA238650.056.431311
GTATCAA413250.034.5515021
ATCAACG526100.027.6924673
TCAACGC526650.027.6708894
CAACGCA539150.027.0132585
TATCAAC549250.026.7147942
AACGCAG555750.026.2306566
CTTATAC114200.024.3830971
TGGTATC95200.024.237882
GTGGTAT105100.023.1928181
ACGCAGA637800.022.849417
CGCAGAG642250.022.7180888
GAGTACT452250.020.77026712-13
TATACAC178150.020.3779053
GCAGAGT746250.019.6217219
CAGAGTA733000.019.45600510-11
GTACTTT499200.018.71294614-15
AGAGTAC684250.018.11177810-11
ACCGAAC19250.017.338918
TACATGG467450.017.1376652
Produced by FastQC (version 0.11.5)