##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139702_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15480598 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.96630110800629 31.0 18.0 33.0 18.0 33.0 2 30.065606315725013 32.0 28.0 33.0 25.0 33.0 3 31.558880025177324 33.0 32.0 33.0 27.0 34.0 4 31.74185751739048 33.0 32.0 33.0 30.0 34.0 5 32.13254739900874 33.0 33.0 34.0 31.0 34.0 6 35.616373798996655 38.0 36.0 38.0 30.0 38.0 7 35.98389532497388 38.0 37.0 38.0 31.0 38.0 8 36.32593385604355 38.0 37.0 38.0 33.0 38.0 9 36.45763781218271 38.0 37.0 38.0 34.0 38.0 10-11 36.56339574220583 38.0 38.0 38.0 34.0 38.0 12-13 36.58612567809073 38.0 38.0 38.0 34.0 38.0 14-15 36.60609351137469 38.0 38.0 38.0 34.0 38.0 16-17 36.60833583431338 38.0 38.0 38.0 34.0 38.0 18-19 36.61877974610542 38.0 38.0 38.0 34.0 38.0 20-21 36.61216581833948 38.0 38.0 38.0 34.0 38.0 22-23 36.60783990940335 38.0 38.0 38.0 34.0 38.0 24-25 36.605671415397694 38.0 38.0 38.0 34.0 38.0 26-27 36.552488723802874 38.0 38.0 38.0 34.0 38.0 28-29 36.52803210973787 38.0 38.0 38.0 34.0 38.0 30-31 36.528707903288165 38.0 38.0 38.0 34.0 38.0 32-33 36.50820403258606 38.0 38.0 38.0 34.0 38.0 34-35 36.47048475215864 38.0 38.0 38.0 34.0 38.0 36-37 36.45302047774079 38.0 38.0 38.0 34.0 38.0 38-39 36.4326299043935 38.0 38.0 38.0 34.0 38.0 40-41 36.433554206442466 38.0 38.0 38.0 34.0 38.0 42-43 36.38095691159674 38.0 38.0 38.0 34.0 38.0 44-45 36.326974548242376 38.0 38.0 38.0 33.0 38.0 46-47 36.28419114863483 38.0 38.0 38.0 33.0 38.0 48-49 36.234588298979034 38.0 37.5 38.0 33.0 38.0 50-51 36.20633923527735 38.0 37.0 38.0 33.0 38.0 52-53 36.19140501812562 38.0 37.0 38.0 33.0 38.0 54-55 36.158190603576806 38.0 37.0 38.0 33.0 38.0 56-57 36.157876476848486 38.0 37.0 38.0 33.0 38.0 58-59 36.13905817641718 38.0 37.0 38.0 33.0 38.0 60-61 36.12716012775968 38.0 37.0 38.0 33.0 38.0 62-63 36.12617323849988 38.0 37.0 38.0 33.0 38.0 64-65 36.11599461425017 38.0 37.0 38.0 33.0 38.0 66-67 36.11862409502248 38.0 37.0 38.0 33.0 38.0 68-69 36.104296336944415 38.0 37.0 38.0 33.0 38.0 70-71 36.0781556343946 38.0 37.0 38.0 33.0 38.0 72-73 36.05147633584835 38.0 37.0 38.0 32.0 38.0 74-75 36.07069889272118 38.0 37.0 38.0 33.0 38.0 76-77 36.053866653782066 38.0 37.0 38.0 32.5 38.0 78-79 36.04009528055708 38.0 37.0 38.0 32.0 38.0 80-81 36.00078344491415 38.0 37.0 38.0 31.0 38.0 82-83 36.003292744892896 38.0 37.0 38.0 31.0 38.0 84-85 35.99740466750052 38.0 37.0 38.0 31.0 38.0 86-87 35.995961066017834 38.0 37.0 38.0 31.0 38.0 88-89 35.96847873333209 38.0 37.0 38.0 31.0 38.0 90-91 35.952355623846714 38.0 37.0 38.0 31.0 38.0 92-93 35.90228844135207 38.0 37.0 38.0 31.0 38.0 94-95 35.89614934354408 38.0 37.0 38.0 31.0 38.0 96-97 35.92767517842232 38.0 37.0 38.0 31.0 38.0 98-99 35.957786020808996 38.0 37.0 38.0 31.0 38.0 100-101 35.43534925049753 38.0 36.0 38.0 30.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 12.0 18 31.0 19 70.0 20 181.0 21 899.0 22 3777.0 23 11755.0 24 27258.0 25 52384.0 26 87224.0 27 131661.0 28 186641.0 29 250352.0 30 321922.0 31 404746.0 32 507519.0 33 647804.0 34 871491.0 35 1299651.0 36 2588181.0 37 8087039.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.02347299503546 23.421304525832916 14.622794287404142 23.932428191727478 2 15.17697184566126 21.51727601220573 40.880494409841276 22.425257732291737 3 17.797208421376308 26.894567422883398 30.79932145272682 24.508902703013476 4 12.904792179216852 19.044076979455184 38.8058135738684 29.245317267459566 5 13.377437217887667 37.51697198136572 35.91450283598835 13.191087964758255 6 29.007111289054293 39.07816345842476 18.569251560517984 13.345473692002962 7 25.664131321025195 33.541327021087945 23.097156841098773 17.697384816788087 8 23.926194582405667 37.69800753175039 20.92168532507594 17.454112560768 9 24.975010655273135 17.21139583884292 21.399780551113075 36.41381295477087 10-11 23.741363221239904 27.33164442355521 29.015154970111617 19.911837385093264 12-13 24.694068665822858 24.81235544001595 29.186592145858963 21.30698374830223 14-15 21.886317494290353 26.22201605732699 26.873912772030454 25.0177536763522 16-17 21.207007675252846 29.406733170344364 27.620871868802222 21.76538728560057 18-19 21.25135282241681 28.26759663935463 29.61099112579501 20.87005941243355 20-21 22.071716562632556 27.248803548803558 29.671637610467705 21.007842278096177 22-23 21.81551828544559 27.258260258727567 29.53055960842169 21.39566184740515 24-25 21.565989308938242 27.456536972498764 29.582279466120802 21.39519425244219 26-27 21.47874693135879 27.539472052513126 29.597905171950856 21.38387584417723 28-29 21.356601589000785 27.486372044219067 29.74823339757675 21.4087929692034 30-31 21.735081431914935 27.334173602347526 29.491038764209947 21.439706201527592 32-33 21.614894869860994 27.497717062755427 29.529489898357085 21.357898169026495 34-35 21.658890834064803 27.626586625438033 29.484843377617747 21.22967916287942 36-37 21.55213486308832 27.476011785924655 29.488871527687277 21.482981823299745 38-39 21.45977343414129 27.546597515423286 29.60032319228717 21.393305858148253 40-41 21.702381160784785 27.447548374588298 29.479308065925984 21.37076239870093 42-43 21.673082271340785 27.515520623548134 29.273375899617115 21.538021205493965 44-45 21.658526711271236 27.6797372578177 29.10759366190187 21.554142369009195 46-47 21.83884463706837 27.579657055154456 28.77743680506987 21.804061502707306 48-49 21.727111879049794 27.719420147618766 28.754163810000442 21.799304163330998 50-51 21.6525530789671 27.86055968572873 28.68556949732896 21.801317737975207 52-53 21.859429495532087 27.794131472907956 28.66447589432462 21.68196313723533 54-55 21.797008039011875 27.768026958887145 28.586925271468694 21.848039730632284 56-57 21.87338736275451 27.90352328332726 28.453008240168405 21.77008111374983 58-59 21.734423916947527 28.074229572245002 28.566596706521302 21.624749804286168 60-61 21.88924830383701 28.143855621842757 28.42281103893154 21.54408503538869 62-63 21.769089344425392 28.26149806893684 28.38349145410365 21.585921132534118 64-65 21.724946097386898 28.208911508510866 28.39835297700738 21.66778941709486 66-67 21.82290986331387 28.213825072156745 28.236124674045822 21.727140390483562 68-69 21.75201967167766 28.092516598992884 28.39090368131543 21.764560048014026 70-71 21.820131298190567 27.77933818048638 28.471312235301323 21.929218286021733 72-73 21.882869906588624 27.78494646915739 28.413032240836205 21.919151383417784 74-75 21.718164645187695 27.910151948081506 28.39868883343456 21.97299457329624 76-77 21.86864311879591 27.848596427961358 28.334871979805264 21.947888473437473 78-79 21.875390860393665 27.89567682866317 28.263884329050605 21.965047981892557 80-81 21.90517471929967 27.886412027479352 28.26014085108107 21.948272402139914 82-83 21.923473543125294 27.855482561575368 28.34747200436712 21.87357189093222 84-85 22.00976349724126 27.874393812039145 28.235726746473137 21.880115944246455 86-87 21.8582874438403 27.905081852590886 28.277673465078045 21.95895723849077 88-89 21.925265921409622 27.90881742235453 28.188851714540714 21.977064941695133 90-91 21.97285510441313 27.904381425532097 28.231900331661564 21.890863138393208 92-93 22.004045400429103 27.900758139872366 28.167187638926684 21.928008820771847 94-95 21.877634249679605 27.98265805999541 28.17109963057195 21.968608059753038 96-97 21.910362944766266 27.97334474210629 28.1946544375599 21.921637875567544 98-99 22.147180222643918 28.56012522627303 28.260675277430416 21.032019273652637 100-101 22.712769989334415 31.055683942088415 25.670979107924907 20.560566960652263 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4394.0 1 4139.5 2 5048.5 3 6513.5 4 7891.0 5 10994.5 6 14181.5 7 18873.5 8 23894.0 9 26497.0 10 25797.5 11 23920.0 12 25442.0 13 31146.0 14 44395.5 15 61297.0 16 73232.5 17 78302.5 18 78599.0 19 75010.0 20 68671.5 21 62986.0 22 62792.5 23 70914.0 24 86789.0 25 111064.5 26 142067.0 27 174125.5 28 205313.5 29 238129.5 30 269092.0 31 300344.5 32 338206.0 33 378083.0 34 415009.5 35 450570.5 36 488697.0 37 521831.5 38 546605.5 39 564600.5 40 583409.0 41 601890.5 42 611434.5 43 620359.5 44 630541.0 45 646091.5 46 653668.5 47 646307.5 48 635381.5 49 616132.0 50 595332.0 51 573448.0 52 541809.5 53 507006.5 54 472686.5 55 433829.5 56 393518.0 57 353015.0 58 311565.5 59 269689.0 60 224096.0 61 179557.0 62 139875.5 63 106174.0 64 79300.0 65 58302.0 66 42428.5 67 31745.0 68 23882.5 69 17314.5 70 12507.5 71 8834.5 72 6075.5 73 3950.0 74 2596.0 75 1713.5 76 1091.5 77 672.0 78 420.5 79 273.5 80 174.0 81 110.5 82 75.0 83 53.5 84 35.5 85 24.5 86 15.5 87 8.5 88 5.0 89 2.5 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.003023139028608585 4 0.0 5 0.05192305878623035 6 6.459698779078173E-6 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.689548168617259E-6 16-17 3.2298493895390864E-6 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.95002246067911E-4 28-29 2.0363226959929886E-4 30-31 0.0 32-33 0.0 34-35 7.793687074501219E-5 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 9.994350859749042E-6 46-47 1.4105335353172576E-4 48-49 0.0 50-51 3.068788008431256E-5 52-53 1.029745503204362E-5 54-55 0.0011161330185907332 56-57 0.00210242140816357 58-59 0.008853125007563212 60-61 0.007901044281199403 62-63 0.0041681651576661915 64-65 0.005109297679459683 66-67 0.0065489018599967945 68-69 0.003682617067361247 70-71 0.0055779797318380704 72-73 0.0010554635661249603 74-75 0.0016025363108959567 76-77 0.0014082972088650145 78-79 0.0011652606812719572 80-81 8.519617348357394E-5 82-83 0.0 84-85 0.0 86-87 3.752448722289214E-4 88-89 9.845630997209892E-4 90-91 0.0 92-93 8.25945832902981E-6 94-95 1.5043877977434183E-4 96-97 2.7107198168231086E-4 98-99 8.744850703973599E-6 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 181.0 20-21 924.0 22-23 1768.0 24-25 2102.0 26-27 4566.0 28-29 9856.0 30-31 22911.0 32-33 31414.0 34-35 43922.0 36-37 67629.0 38-39 80469.0 40-41 81631.0 42-43 96186.0 44-45 118192.0 46-47 120438.0 48-49 109712.0 50-51 98636.0 52-53 95021.0 54-55 103490.0 56-57 108967.0 58-59 110657.0 60-61 109928.0 62-63 108038.0 64-65 119603.0 66-67 130066.0 68-69 130386.0 70-71 138652.0 72-73 115077.0 74-75 117349.0 76-77 125688.0 78-79 132009.0 80-81 133940.0 82-83 126860.0 84-85 126712.0 86-87 131960.0 88-89 139358.0 90-91 143821.0 92-93 141420.0 94-95 150280.0 96-97 240076.0 98-99 1291847.0 100-101 1.0318856E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.58504262714851 #Duplication Level Percentage of deduplicated Percentage of total 1 88.15271684313963 72.80095878189638 2 8.099039708179545 13.377190790779522 3 2.0226868255060464 5.0113103311736555 4 0.7871111410956708 2.6001442855875787 5 0.3682524727365469 1.5206073079250282 6 0.1958041405751584 0.970229597758298 7 0.10370394084428737 0.5995076062660132 8 0.0687245848188488 0.45404982134381694 9 0.04352746025197257 0.32352454443246004 >10 0.15386431018948712 1.9165361282553768 >50 0.0034797957758684428 0.197714655941566 >100 0.0010185774537622143 0.15882142444818345 >500 2.8082926603562288E-5 0.01695356720496731 >1k 4.211650661672581E-5 0.05245115698701257 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.459698779078173E-6 0.0 2 0.0 4.521789145354721E-5 0.0 1.2919397558156346E-5 0.0 3 6.459698779078173E-6 4.521789145354721E-5 0.0 1.2919397558156346E-5 0.0 4 6.459698779078173E-6 6.459698779078172E-5 0.0 1.9379096337234517E-5 0.0 5 6.459698779078173E-6 7.10566865698599E-5 0.0 1.9379096337234517E-5 0.0 6 6.459698779078173E-6 7.10566865698599E-5 0.0 3.8758192674469035E-5 0.0 7 6.459698779078173E-6 7.10566865698599E-5 0.0 3.8758192674469035E-5 0.0 8 6.459698779078173E-6 7.751638534893807E-5 0.0 4.521789145354721E-5 0.0 9 6.459698779078173E-6 1.2273427680248528E-4 0.0 6.459698779078172E-5 0.0 10-11 6.459698779078173E-6 1.2273427680248528E-4 0.0 8.720593351755533E-5 0.0 12-13 6.459698779078173E-6 1.2273427680248528E-4 0.0 1.2596412619202436E-4 0.0 14-15 6.459698779078173E-6 1.5826262008741522E-4 0.0 1.356536743606416E-4 0.0 16-17 6.459698779078173E-6 1.614924694769543E-4 0.0 1.5180292130833706E-4 0.0 18-19 6.459698779078173E-6 1.7118201764557156E-4 0.0 1.679521682560325E-4 6.459698779078173E-6 20-21 6.459698779078173E-6 1.9056111398280608E-4 0.0 1.7764171642464975E-4 1.2919397558156346E-5 22-23 6.459698779078173E-6 2.390088548258924E-4 0.0 1.9379096337234517E-4 1.614924694769543E-5 24-25 6.459698779078173E-6 2.7453719811082233E-4 0.0 2.2608945726773606E-4 1.9379096337234517E-5 26-27 6.459698779078173E-6 2.842267462794396E-4 0.0 2.7776704750036144E-4 1.9379096337234517E-5 28-29 6.459698779078173E-6 2.842267462794396E-4 0.0 5.23235601105332E-4 1.9379096337234517E-5 30-31 6.459698779078173E-6 3.4236403529114317E-4 0.0 0.0011110681900014456 1.9379096337234517E-5 32-33 9.689548168617259E-6 3.4236403529114317E-4 0.0 0.0022608945726773602 1.9379096337234517E-5 34-35 1.2919397558156346E-5 3.811222279656122E-4 0.0 0.0038693595686678257 1.9379096337234517E-5 36-37 1.2919397558156346E-5 4.231102700296203E-4 0.0 0.006440319682740938 2.583879511631269E-5 38-39 1.2919397558156346E-5 4.457192157563939E-4 0.0 0.010345207594693692 2.583879511631269E-5 40-41 1.2919397558156346E-5 5.038565047680974E-4 0.0 0.014644137132170217 2.583879511631269E-5 42-43 1.2919397558156346E-5 5.103162035471757E-4 0.0 0.01851995639961712 2.583879511631269E-5 44-45 1.2919397558156346E-5 5.61993793779801E-4 0.0 0.023319512592472204 2.583879511631269E-5 46-47 1.2919397558156346E-5 6.072116852333482E-4 0.0 0.028438823874891654 2.583879511631269E-5 48-49 1.2919397558156346E-5 6.072116852333482E-4 0.0 0.03381652310847423 2.583879511631269E-5 50-51 1.2919397558156346E-5 7.170265644776772E-4 0.0 0.03891322544516691 2.583879511631269E-5 52-53 1.2919397558156346E-5 7.234862632567553E-4 0.0 0.04382582636665586 2.583879511631269E-5 54-55 1.2919397558156346E-5 7.267161126462944E-4 0.0 0.049116319666720885 2.583879511631269E-5 56-57 1.2919397558156346E-5 7.299459620358334E-4 0.0 0.05457476513504194 2.583879511631269E-5 58-59 1.2919397558156346E-5 7.364056608149117E-4 0.0 0.059904016627781435 2.9068644505851778E-5 60-61 1.2919397558156346E-5 7.46095208983529E-4 0.0 0.06507823534982304 3.5528343284929945E-5 62-63 1.2919397558156346E-5 7.557847571521462E-4 0.0 0.07034934955355084 3.8758192674469035E-5 64-65 1.2919397558156346E-5 8.333011425010842E-4 0.0 0.07564630255239493 3.8758192674469035E-5 66-67 1.2919397558156346E-5 8.429906906697015E-4 0.0 0.08110474802071599 3.8758192674469035E-5 68-69 1.2919397558156346E-5 8.882085821232487E-4 0.0 0.08646629800735088 3.8758192674469035E-5 70-71 1.2919397558156346E-5 9.205070760186396E-4 0.0 0.09148225410930508 3.8758192674469035E-5 72-73 1.2919397558156346E-5 9.36656322966335E-4 0.0 0.09539683156942645 3.8758192674469035E-5 74-75 1.2919397558156346E-5 9.78644365030343E-4 0.0 0.0984910272846049 3.8758192674469035E-5 76-77 1.2919397558156346E-5 0.0010206324070943513 0.0 0.10166919908391137 3.8758192674469035E-5 78-79 1.2919397558156346E-5 0.0010335518046525076 0.0 0.10488612907589229 3.8758192674469035E-5 80-81 1.2919397558156346E-5 0.0010367816540420467 0.0 0.10623943597010915 3.8758192674469035E-5 82-83 1.2919397558156346E-5 0.0010400115034315859 0.0 0.10673360292670865 3.8758192674469035E-5 84-85 1.2919397558156346E-5 0.0010400115034315859 0.0 0.10727621762415121 3.8758192674469035E-5 86-87 1.2919397558156346E-5 0.0010529309009897421 0.0 0.10746354888874449 3.8758192674469035E-5 88-89 1.2919397558156346E-5 0.0011046084912223674 0.0 0.1075152264789771 3.8758192674469035E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 23865 0.0 56.43131 1 GTATCAA 41325 0.0 34.551502 1 ATCAACG 52610 0.0 27.692467 3 TCAACGC 52665 0.0 27.670889 4 CAACGCA 53915 0.0 27.013258 5 TATCAAC 54925 0.0 26.714794 2 AACGCAG 55575 0.0 26.230656 6 CTTATAC 11420 0.0 24.383097 1 TGGTATC 9520 0.0 24.23788 2 GTGGTAT 10510 0.0 23.192818 1 ACGCAGA 63780 0.0 22.84941 7 CGCAGAG 64225 0.0 22.718088 8 GAGTACT 45225 0.0 20.770267 12-13 TATACAC 17815 0.0 20.377905 3 GCAGAGT 74625 0.0 19.621721 9 CAGAGTA 73300 0.0 19.456005 10-11 GTACTTT 49920 0.0 18.712946 14-15 AGAGTAC 68425 0.0 18.111778 10-11 ACCGAAC 1925 0.0 17.33891 8 TACATGG 46745 0.0 17.137665 2 >>END_MODULE