FastQCFastQC Report
Fri 27 May 2016
SRR2139701_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139701_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11334944
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA198950.054.128981
GTATCAA368850.037.269321
TGGTATC81450.032.0952452
GTGGTAT84150.030.557011
TCAACGC466200.030.0641374
ATCAACG468050.029.9553743
TATCAAC472400.029.7701032
CAACGCA476250.029.4297125
AACGCAG490700.028.5910226
ACGCAGA563400.024.9396427
CGCAGAG572600.024.591218
GCAGAGT651750.021.5654349
GAGTACT374650.020.57829912-13
CAGAGTA643300.019.57210410-11
GTACTTT407300.018.87654314-15
AGAGTAC607350.018.28757310-11
TACATGG446550.018.1084922
GTACATG444300.017.959451
ACATGGG452150.017.3732343
AGTACTT401100.017.01452312-13
Produced by FastQC (version 0.11.5)