##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139701_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11334944
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.203494962127735	32.0	25.0	33.0	18.0	33.0
2	30.118858284610845	32.0	29.0	33.0	25.0	33.0
3	31.570339826998705	33.0	32.0	33.0	27.0	34.0
4	31.727015678242434	33.0	32.0	33.0	30.0	34.0
5	32.10221982570007	33.0	33.0	34.0	31.0	34.0
6	35.56887868171206	38.0	36.0	38.0	30.0	38.0
7	35.935325397284714	38.0	37.0	38.0	31.0	38.0
8	36.283930207330535	38.0	37.0	38.0	33.0	38.0
9	36.423000766479305	38.0	37.0	38.0	33.0	38.0
10-11	36.52988117982762	38.0	38.0	38.0	34.0	38.0
12-13	36.55434376208652	38.0	38.0	38.0	34.0	38.0
14-15	36.580880858343896	38.0	38.0	38.0	34.0	38.0
16-17	36.587272994026264	38.0	38.0	38.0	34.0	38.0
18-19	36.598921485628864	38.0	38.0	38.0	34.0	38.0
20-21	36.59178879374065	38.0	38.0	38.0	34.0	38.0
22-23	36.59007949764338	38.0	38.0	38.0	34.0	38.0
24-25	36.591057765811286	38.0	38.0	38.0	34.0	38.0
26-27	36.53861420877466	38.0	38.0	38.0	34.0	38.0
28-29	36.51331425232996	38.0	38.0	38.0	34.0	38.0
30-31	36.51255021364014	38.0	38.0	38.0	34.0	38.0
32-33	36.49575303874521	38.0	38.0	38.0	34.0	38.0
34-35	36.46487562944101	38.0	38.0	38.0	34.0	38.0
36-37	36.445076526940895	38.0	38.0	38.0	34.0	38.0
38-39	36.430060382948575	38.0	38.0	38.0	34.0	38.0
40-41	36.42591437877404	38.0	38.0	38.0	34.0	38.0
42-43	36.371273205579605	38.0	38.0	38.0	33.5	38.0
44-45	36.31759782213374	38.0	38.0	38.0	33.0	38.0
46-47	36.27402607273545	38.0	38.0	38.0	33.0	38.0
48-49	36.2206643530013	38.0	37.5	38.0	33.0	38.0
50-51	36.1937775688729	38.0	37.0	38.0	33.0	38.0
52-53	36.177218066912104	38.0	37.0	38.0	33.0	38.0
54-55	36.14489239432359	38.0	37.0	38.0	33.0	38.0
56-57	36.14762919947018	38.0	37.0	38.0	33.0	38.0
58-59	36.13113530983502	38.0	37.0	38.0	33.0	38.0
60-61	36.12036589647549	38.0	37.0	38.0	33.0	38.0
62-63	36.11791485910818	38.0	37.0	38.0	33.0	38.0
64-65	36.109587359662626	38.0	37.0	38.0	33.0	38.0
66-67	36.11534717300119	38.0	37.0	38.0	33.0	38.0
68-69	36.1038990367211	38.0	37.0	38.0	33.0	38.0
70-71	36.07744997549866	38.0	37.0	38.0	33.0	38.0
72-73	36.05188415892043	38.0	37.0	38.0	32.0	38.0
74-75	36.073134887558396	38.0	37.0	38.0	33.0	38.0
76-77	36.056345587210146	38.0	37.0	38.0	32.5	38.0
78-79	36.04346602435006	38.0	37.0	38.0	32.0	38.0
80-81	36.00241857722182	38.0	37.0	38.0	31.0	38.0
82-83	36.00453390839334	38.0	37.0	38.0	31.0	38.0
84-85	36.00067835705197	38.0	37.0	38.0	31.0	38.0
86-87	35.9981987611549	38.0	37.0	38.0	31.0	38.0
88-89	35.972584883514045	38.0	37.0	38.0	31.0	38.0
90-91	35.95187862213349	38.0	37.0	38.0	31.0	38.0
92-93	35.90100592111187	38.0	37.0	38.0	31.0	38.0
94-95	35.894713639893894	38.0	37.0	38.0	31.0	38.0
96-97	35.92468339650507	38.0	37.0	38.0	31.0	38.0
98-99	35.9569198360949	38.0	37.0	38.0	31.0	38.0
100-101	35.438688490390916	38.0	36.0	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	20.0
19	40.0
20	114.0
21	603.0
22	2829.0
23	8503.0
24	20202.0
25	37905.0
26	63546.0
27	96950.0
28	137323.0
29	184690.0
30	237869.0
31	300034.0
32	376174.0
33	477741.0
34	642206.0
35	958426.0
36	1913323.0
37	5876444.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.23956748264482	22.603667031791247	14.494981183850578	23.661784301713357
2	15.657536552452312	21.0812333964773	40.73799570602201	22.523234345048373
3	18.57298461490684	26.606239933485128	29.85600384239137	24.96477160921666
4	13.347600129299272	18.386354621602013	38.02623109562782	30.239814153470896
5	13.777061239729493	37.047540573378534	35.249526333141056	13.925871853750916
6	29.849243189909007	38.07707386997236	18.15248491743762	13.921198022681011
7	26.319415428960212	33.17572632030648	22.451976824940644	18.052881425792663
8	24.3192996807042	37.05603662444208	20.474940149682258	18.14972354517146
9	25.364448205478563	17.23325673245496	21.20382773836377	36.1984673237027
10-11	24.21249721216091	27.13078247232629	28.129900774101753	20.526819541411058
12-13	25.29196880019875	24.604051859453385	28.578707578969954	21.525271761377912
14-15	22.43978002890883	25.84710167072727	26.597872914061156	25.11524538630275
16-17	21.402752408834132	29.09686629241397	27.314215226824235	22.18616607192766
18-19	21.406890056095556	27.778434547184354	29.629537649237612	21.185137747482475
20-21	22.5673886316074	26.69331387558614	29.615985784469395	21.123311708337063
22-23	22.211407022313615	26.642110712138855	29.57521998496009	21.57126228058744
24-25	21.807951871156845	26.926327130021587	29.640766316833894	21.624954681987674
26-27	21.737139570802917	26.983160174568148	29.633196032856617	21.646504221772318
28-29	21.572994923786148	26.926547929097953	29.754087170722947	21.74636997639295
30-31	21.952875312154355	26.927404789771934	29.597453380956463	21.52226651711725
32-33	21.65167741731991	27.10137438959432	29.602044201185883	21.644903991899884
34-35	21.755835716715257	27.040039572373836	29.621147122579462	21.582977588331445
36-37	21.87369197616297	27.05288862076925	29.421958195167797	21.65146120789998
38-39	21.681192635328276	27.11596290059749	29.573319167568297	21.629525296505935
40-41	21.874809895305813	26.94997813880759	29.482970370649582	21.692241595237014
42-43	21.81196412951767	27.062396586198478	29.239279585759352	21.886359698524497
44-45	21.88323078635203	27.22115172321965	28.991240565935694	21.904376924492624
46-47	22.071470656648327	27.263717817132722	28.544234483711968	22.120577042506987
48-49	21.970045537199656	27.404764216362903	28.43788972671583	22.18730051972161
50-51	22.024641906747206	27.51914350032117	28.2816602514608	22.17455434147083
52-53	22.173105338636965	27.487326975807264	28.19342511168414	22.14614257387163
54-55	22.111447106828162	27.544832623957106	28.140951920700374	22.202768348514358
56-57	22.12805348388695	27.61162973736464	28.065033157606962	22.19528362114145
58-59	22.008874039033053	27.59422551506361	28.25665247179166	22.14024797411168
60-61	22.17048263149818	27.7075133404809	28.092363428910684	22.029640599110238
62-63	22.104391019193656	27.793600986271468	28.066598219851702	22.035409774683178
64-65	22.042992092960763	27.68018744843849	28.08772205193758	22.18909840666316
66-67	22.100815314480688	27.73063112153939	27.91029481965529	22.25825874432463
68-69	22.120999064051038	27.667229164890934	28.01805123460028	22.193720536457754
70-71	22.157600639794047	27.41217534531608	28.115392787075894	22.31483122781398
72-73	22.143616440181503	27.495179244559264	28.06588305628861	22.29532125897062
74-75	22.030431598588056	27.612106079815796	28.024307986900933	22.333154334695216
76-77	22.17364309232227	27.542559484763995	27.93864924122645	22.345148181687286
78-79	22.18109117911153	27.577771825640102	27.883047533757132	22.358089461491236
80-81	22.165414344601782	27.601690239948066	27.88478201318056	22.348113402269593
82-83	22.195882772109236	27.562734465857496	27.978971068536072	22.262411693497192
84-85	22.269008276602506	27.524950213080007	27.916429639086004	22.289611871231486
86-87	22.16683767327986	27.62503023966006	27.902577178191816	22.305554908868263
88-89	22.228105035321523	27.571891325043307	27.867145354517525	22.332858285117645
90-91	22.241767300127457	27.579225183927836	27.89167979327066	22.287327722674046
92-93	22.294935339378842	27.630604574885453	27.792969737584993	22.281490348150708
94-95	22.138329852663194	27.65244960772512	27.854349507005143	22.35487103260654
96-97	22.191301284675376	27.683939139447993	27.852513285915826	22.272246289960805
98-99	22.473356861344534	28.22657430329202	27.9213473849354	21.378721450428042
100-101	23.043484438548845	30.784756836677218	25.215512989959166	20.956245734814768
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3578.0
1	3589.0
2	4266.0
3	5133.0
4	6689.5
5	10242.5
6	13198.0
7	15970.0
8	18402.5
9	18990.5
10	18129.5
11	17158.5
12	18812.5
13	25079.0
14	40240.0
15	59453.0
16	73412.5
17	79902.0
18	79126.5
19	72462.5
20	62242.5
21	51639.5
22	45436.5
23	46364.5
24	53956.0
25	68269.0
26	87788.0
27	109004.0
28	131170.5
29	155063.0
30	178576.0
31	202666.5
32	229698.5
33	258420.0
34	284809.0
35	310225.5
36	340081.0
37	365781.5
38	384495.0
39	400007.0
40	415218.0
41	431311.0
42	442568.0
43	451884.5
44	466227.0
45	476461.5
46	477006.0
47	474950.0
48	468990.0
49	461524.0
50	454856.0
51	443084.5
52	424836.5
53	404409.0
54	382980.0
55	359459.5
56	333183.5
57	303368.5
58	270666.5
59	234315.5
60	195157.0
61	156784.0
62	121876.0
63	92807.5
64	68944.5
65	50150.0
66	36534.5
67	27189.0
68	20267.5
69	14573.5
70	10249.0
71	7119.5
72	4877.0
73	3208.0
74	2051.0
75	1295.5
76	825.0
77	504.5
78	314.5
79	216.5
80	147.5
81	98.0
82	63.5
83	36.5
84	23.0
85	18.0
86	13.5
87	10.0
88	8.0
89	7.5
90	8.5
91	6.0
92	3.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0029642846051996376
4	0.0
5	0.05098393075431162
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.64951541310549E-4
28-29	2.295885208565277E-4
30-31	0.0
32-33	0.0
34-35	8.885472128673009E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	4.592992434698441E-6
46-47	1.2997230522269107E-4
48-49	0.0
50-51	4.7331300359320304E-6
52-53	2.385240475412774E-5
54-55	0.001115361503807301
56-57	0.002235136343316942
58-59	0.0086727159145511
60-61	0.007645182207543648
62-63	0.004077763497583888
64-65	0.005007415193371608
66-67	0.006576401791478778
68-69	0.0036469535849348853
70-71	0.00562707951518905
72-73	0.0010040688320561526
74-75	0.0016101171526519342
76-77	0.0013806790019407337
78-79	0.0012336287631805017
80-81	9.258297626711648E-5
82-83	0.0
84-85	0.0
86-87	3.317775963185508E-4
88-89	7.734164922877865E-4
90-91	0.0
92-93	0.0
94-95	1.7100137461518794E-4
96-97	4.2448267817720206E-4
98-99	1.8524044858567992E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	187.0
20-21	622.0
22-23	1385.0
24-25	1881.0
26-27	4222.0
28-29	9840.0
30-31	23191.0
32-33	30058.0
34-35	41475.0
36-37	63731.0
38-39	76167.0
40-41	77866.0
42-43	90997.0
44-45	112536.0
46-47	114050.0
48-49	100855.0
50-51	85261.0
52-53	79181.0
54-55	86313.0
56-57	90826.0
58-59	90689.0
60-61	89655.0
62-63	86108.0
64-65	92863.0
66-67	98793.0
68-69	100289.0
70-71	102236.0
72-73	89968.0
74-75	90559.0
76-77	96610.0
78-79	100256.0
80-81	101033.0
82-83	95472.0
84-85	94297.0
86-87	97728.0
88-89	102847.0
90-91	106023.0
92-93	103292.0
94-95	109364.0
96-97	172570.0
98-99	924863.0
100-101	7298785.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.68550391843924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.04958550768568	77.1594411187263
2	7.04502850706015	12.073136354944374
3	1.6736492829838732	4.302224465856231
4	0.572277844791772	1.9614366204936537
5	0.26100390333974827	1.1182125491172967
6	0.13328210331594875	0.6852206511561929
7	0.0761295183347284	0.45662372991054834
8	0.049859236042846235	0.34177710122557503
9	0.031667599601089357	0.2442108806736036
>10	0.10424653133762711	1.390620711239697
>50	0.002543461107938428	0.1445407535602339
>100	6.93126941388293E-4	0.09793078870812308
>500	3.3377457207870036E-5	0.0246242743880569
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.7644551221426413E-5	0.0	0.0	0.0
5	0.0	1.7644551221426413E-5	0.0	0.0	8.822275610713206E-6
6	3.5289102442852826E-5	1.7644551221426413E-5	0.0	0.0	8.822275610713206E-6
7	3.5289102442852826E-5	1.7644551221426413E-5	0.0	1.7644551221426413E-5	8.822275610713206E-6
8	3.5289102442852826E-5	1.7644551221426413E-5	0.0	1.7644551221426413E-5	8.822275610713206E-6
9	3.5289102442852826E-5	7.940048049641886E-5	0.0	3.5289102442852826E-5	8.822275610713206E-6
10-11	3.5289102442852826E-5	7.940048049641886E-5	0.0	6.175592927499245E-5	8.822275610713206E-6
12-13	3.5289102442852826E-5	8.822275610713207E-5	0.0	1.0586730732855848E-4	8.822275610713206E-6
14-15	3.970024024820943E-5	1.1027844513391508E-4	0.0	1.235118585499849E-4	2.646682683213962E-5
16-17	7.498934269106226E-5	1.146895829392717E-4	0.0	1.323341341606981E-4	3.5289102442852826E-5
18-19	7.940048049641886E-5	1.323341341606981E-4	0.0	1.8526778782497735E-4	3.5289102442852826E-5
20-21	7.940048049641886E-5	1.5880096099283773E-4	0.0	2.293791658785434E-4	3.5289102442852826E-5
22-23	8.822275610713207E-5	1.7203437440890752E-4	0.0	2.7349054393210946E-4	3.970024024820943E-5
24-25	8.822275610713207E-5	1.9409006343569057E-4	0.0	3.1760192198567545E-4	4.852251585892264E-5
26-27	8.822275610713207E-5	2.0291233904640377E-4	0.0	3.793578512606679E-4	6.175592927499245E-5
28-29	8.822275610713207E-5	2.0291233904640377E-4	0.0	7.013709110517E-4	6.175592927499245E-5
30-31	8.822275610713207E-5	2.470237170999698E-4	0.0	0.0016497655392033695	6.175592927499245E-5
32-33	8.822275610713207E-5	2.470237170999698E-4	0.0	0.003281886527185313	7.057820488570565E-5
34-35	1.0145616952320189E-4	2.9113509515353586E-4	0.0	0.005297776504233281	7.940048049641886E-5
36-37	1.0586730732855848E-4	3.3524647320710185E-4	0.0	0.008910498366820339	8.822275610713207E-5
38-39	1.0586730732855848E-4	3.528910244285283E-4	0.0	0.014406776072294666	9.263389391248867E-5
40-41	1.0586730732855848E-4	3.661244378445981E-4	0.0	0.02037504552294215	9.704503171784527E-5
42-43	1.0586730732855848E-4	3.9259126467673774E-4	0.0	0.025778689334503987	9.704503171784527E-5
44-45	1.0586730732855848E-4	4.675806073678E-4	0.0	0.03208661639616393	1.0145616952320189E-4
46-47	1.0586730732855848E-4	5.205142610320792E-4	0.0	0.03859745579687028	1.0586730732855848E-4
48-49	1.0586730732855848E-4	5.205142610320792E-4	0.0	0.04506418381952306	1.146895829392717E-4
50-51	1.1910072074462831E-4	5.77859052501715E-4	0.0	0.05218376023736862	1.146895829392717E-4
52-53	1.235118585499849E-4	5.822701903070716E-4	0.0	0.05874753329173925	1.1910072074462831E-4
54-55	1.235118585499849E-4	5.866813281124282E-4	0.0	0.06550539640954556	1.279229963553415E-4
56-57	1.235118585499849E-4	5.999147415284981E-4	0.0	0.07254998348470006	1.323341341606981E-4
58-59	1.235118585499849E-4	6.043258793338547E-4	0.0	0.07883144371952786	1.367452719660547E-4
60-61	1.323341341606981E-4	6.175592927499245E-4	0.0	0.08534669425803956	1.411564097714113E-4
62-63	1.411564097714113E-4	6.175592927499245E-4	0.0	0.0916766770087263	1.411564097714113E-4
64-65	1.411564097714113E-4	6.793152220249169E-4	0.0	0.09759642394351485	1.411564097714113E-4
66-67	1.411564097714113E-4	6.969597732463433E-4	0.0	0.10386023962712124	1.411564097714113E-4
68-69	1.411564097714113E-4	7.057820488570566E-4	0.0	0.10974028632166158	1.4556754757676793E-4
70-71	1.5438982318748113E-4	7.057820488570566E-4	0.0	0.11557181050034301	1.9409006343569055E-4
72-73	1.6762323660355092E-4	7.101931866624132E-4	0.0	0.1201064601642496	2.0291233904640377E-4
74-75	1.6762323660355092E-4	7.23426600078483E-4	0.0	0.12399267257076876	2.0291233904640377E-4
76-77	1.7203437440890752E-4	7.322488756891962E-4	0.0	0.12707605789671303	2.0732347685176037E-4
78-79	1.7644551221426415E-4	7.322488756891962E-4	0.0	0.1297933187848127	2.1173461465711697E-4
80-81	1.7644551221426415E-4	7.410711512999094E-4	0.0	0.13078141365321258	2.1614575246247357E-4
82-83	1.7644551221426415E-4	7.498934269106226E-4	0.0	0.13119164946911074	2.382014414892566E-4
84-85	1.7644551221426415E-4	7.631268403266924E-4	0.0	0.13167687462769997	2.382014414892566E-4
86-87	1.7644551221426415E-4	7.851825293534754E-4	0.0	0.13188860924235707	2.382014414892566E-4
88-89	1.7644551221426415E-4	8.292939074070415E-4	0.0	0.131937131758216	2.4261257929461322E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19895	0.0	54.12898	1
GTATCAA	36885	0.0	37.26932	1
TGGTATC	8145	0.0	32.095245	2
GTGGTAT	8415	0.0	30.55701	1
TCAACGC	46620	0.0	30.064137	4
ATCAACG	46805	0.0	29.955374	3
TATCAAC	47240	0.0	29.770103	2
CAACGCA	47625	0.0	29.429712	5
AACGCAG	49070	0.0	28.591022	6
ACGCAGA	56340	0.0	24.939642	7
CGCAGAG	57260	0.0	24.59121	8
GCAGAGT	65175	0.0	21.565434	9
GAGTACT	37465	0.0	20.578299	12-13
CAGAGTA	64330	0.0	19.572104	10-11
GTACTTT	40730	0.0	18.876543	14-15
AGAGTAC	60735	0.0	18.287573	10-11
TACATGG	44655	0.0	18.108492	2
GTACATG	44430	0.0	17.95945	1
ACATGGG	45215	0.0	17.373234	3
AGTACTT	40110	0.0	17.014523	12-13
>>END_MODULE