FastQCFastQC Report
Fri 27 May 2016
SRR2139700_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139700_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12868741
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA170100.050.7129941
GTATCAA306750.035.144881
TCAACGC371650.029.6575054
ATCAACG376450.029.2685683
CAACGCA384300.028.6812735
TATCAAC388350.028.4606462
AACGCAG396900.027.8315356
ACGCAGA456700.024.1872927
CGCAGAG464350.023.8631558
TGGTATC78500.022.4947782
GTGGTAT81550.022.129951
GCAGAGT541550.020.4374689
GAGTACT330500.019.89066512-13
CAGAGTA533100.018.73383910-11
CTTATAC89450.018.6309531
AGAGTAC496800.017.95300710-11
GTACTTT363400.017.92993714-15
TACATGG360300.017.4951112
GTACATG355250.016.9781511
ACATGGG361850.016.8235873
Produced by FastQC (version 0.11.5)