##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139700_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12868741
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.801600871445	31.0	18.0	33.0	18.0	33.0
2	29.965589563112662	32.0	28.0	33.0	25.0	33.0
3	31.520914205981768	33.0	32.0	33.0	27.0	34.0
4	31.708647722415115	33.0	32.0	33.0	29.0	34.0
5	32.10552850508064	33.0	33.0	34.0	31.0	34.0
6	35.588948988871564	38.0	36.0	38.0	30.0	38.0
7	35.96447305917494	38.0	37.0	38.0	31.0	38.0
8	36.31384678578891	38.0	37.0	38.0	33.0	38.0
9	36.44890195552152	38.0	37.0	38.0	34.0	38.0
10-11	36.55684887900067	38.0	38.0	38.0	34.0	38.0
12-13	36.57885942377735	38.0	38.0	38.0	34.0	38.0
14-15	36.60149085291249	38.0	38.0	38.0	34.0	38.0
16-17	36.603495866456555	38.0	38.0	38.0	34.0	38.0
18-19	36.610573598458465	38.0	38.0	38.0	34.0	38.0
20-21	36.604000720781556	38.0	38.0	38.0	34.0	38.0
22-23	36.602519481901396	38.0	38.0	38.0	34.0	38.0
24-25	36.603660796461625	38.0	38.0	38.0	34.0	38.0
26-27	36.550543416921506	38.0	38.0	38.0	34.0	38.0
28-29	36.524843202741	38.0	38.0	38.0	34.0	38.0
30-31	36.52343570476606	38.0	38.0	38.0	34.0	38.0
32-33	36.50647686592595	38.0	38.0	38.0	34.0	38.0
34-35	36.47345701252171	38.0	38.0	38.0	34.0	38.0
36-37	36.454680122689574	38.0	38.0	38.0	34.0	38.0
38-39	36.43727335750846	38.0	38.0	38.0	34.0	38.0
40-41	36.43756960483127	38.0	38.0	38.0	34.0	38.0
42-43	36.38754815242862	38.0	38.0	38.0	34.0	38.0
44-45	36.34104740171001	38.0	38.0	38.0	33.0	38.0
46-47	36.30656742713474	38.0	38.0	38.0	33.0	38.0
48-49	36.260503516620204	38.0	38.0	38.0	33.0	38.0
50-51	36.23455904245375	38.0	37.0	38.0	33.0	38.0
52-53	36.21846853273006	38.0	37.0	38.0	33.0	38.0
54-55	36.18489511308637	38.0	37.0	38.0	33.0	38.0
56-57	36.18462270273655	38.0	37.0	38.0	33.0	38.0
58-59	36.16573350461114	38.0	37.0	38.0	33.0	38.0
60-61	36.15264477770671	38.0	37.0	38.0	33.0	38.0
62-63	36.14798747083094	38.0	37.0	38.0	33.0	38.0
64-65	36.13889822459345	38.0	37.0	38.0	33.0	38.0
66-67	36.14072149869031	38.0	37.0	38.0	33.0	38.0
68-69	36.127214934062145	38.0	37.0	38.0	33.0	38.0
70-71	36.09713345892828	38.0	37.0	38.0	33.0	38.0
72-73	36.07173476088147	38.0	37.0	38.0	32.5	38.0
74-75	36.089863444997334	38.0	37.0	38.0	33.0	38.0
76-77	36.07092699187264	38.0	37.0	38.0	32.5	38.0
78-79	36.05665677090026	38.0	37.0	38.0	32.5	38.0
80-81	36.01401391567581	38.0	37.0	38.0	31.5	38.0
82-83	36.01509217688394	38.0	37.0	38.0	31.0	38.0
84-85	36.01209752269244	38.0	37.0	38.0	31.0	38.0
86-87	36.00722382495053	38.0	37.0	38.0	31.0	38.0
88-89	35.977468873202994	38.0	37.0	38.0	31.0	38.0
90-91	35.95895723463788	38.0	37.0	38.0	31.0	38.0
92-93	35.906919820699734	38.0	37.0	38.0	31.0	38.0
94-95	35.90190216196553	38.0	37.0	38.0	31.0	38.0
96-97	35.93088566501574	38.0	37.0	38.0	31.0	38.0
98-99	35.96356887545062	38.0	37.0	38.0	31.0	38.0
100-101	35.4385418409759	38.0	36.0	38.0	30.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	20.0
19	41.0
20	144.0
21	693.0
22	3126.0
23	9738.0
24	22673.0
25	42866.0
26	71122.0
27	107680.0
28	152880.0
29	204464.0
30	263577.0
31	333375.0
32	418886.0
33	540736.0
34	727468.0
35	1087063.0
36	2180190.0
37	6701997.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	37.87333974628909	23.43188816994607	14.320320845683352	24.374451238081488
2	15.350530405421944	21.406585150792917	40.6748103796634	22.568074064121735
3	18.289083436778316	26.678967644191324	29.974509560430185	25.057439358600174
4	13.274196753202197	18.690849400108373	38.38191319570423	29.653040650985208
5	13.805168770925711	37.0539010219739	35.6162273731769	13.524702833923488
6	29.55683077311137	38.4529302439143	18.534563715284968	13.455675267689356
7	26.070312550388575	32.89641154484343	23.111398387767693	17.921877517000304
8	23.739851474204045	37.62899571916165	20.946174921074252	17.684977885560055
9	24.969800853090447	17.397871322454932	21.428724068655978	36.203603755798646
10-11	23.957110489674164	27.204658171300515	28.756247406020524	20.08198393300479
12-13	24.84161426514062	24.581693733676044	29.021883337305493	21.554808663877843
14-15	22.014113269830613	26.001061486983186	26.646291711542858	25.33853353164335
16-17	21.536311187563385	29.10858292620012	27.446232986048635	21.908872900187863
18-19	21.536947553766137	28.12388950869397	29.244243861928688	21.094919075611205
20-21	22.2480294499245	27.225366336744454	29.268621146478125	21.25798306685292
22-23	22.00065023141423	27.182758352845006	29.11294780783536	21.703643607905406
24-25	21.832600831560757	27.35001874649086	29.170868823684128	21.646511598264258
26-27	21.75725105990237	27.49429437314945	29.128393521142605	21.620061045805574
28-29	21.665036565017143	27.456048007611418	29.231927596658615	21.646987830712828
30-31	22.00003907235383	27.391101574966108	29.037942913734394	21.570916438945673
32-33	21.868695578230167	27.406946671319893	29.040275740541166	21.684082009908778
34-35	21.901479883437897	27.462096132310087	29.055268659887588	21.581155324364428
36-37	21.838727001054846	27.444301000355797	29.007705575843023	21.709266422746335
38-39	21.712032156858633	27.49296959169698	29.10609743250826	21.68890081893613
40-41	21.965934213871158	27.352645714054685	29.002292237075867	21.67912783499829
42-43	21.90712598913074	27.432153360297818	28.80593363466103	21.85478701591041
44-45	21.902940864076566	27.55662871477922	28.69923120150519	21.841199219639023
46-47	22.043113620017877	27.517783302478126	28.396322069848352	22.04278100765564
48-49	21.954735112621883	27.640926243578978	28.37521654278925	22.02912210100989
50-51	21.914907465969833	27.758424123109332	28.302732005762714	22.023936405158118
52-53	22.09446204017057	27.706469656538097	28.260067586355625	21.939000716935706
54-55	22.031597180385827	27.637552046802362	28.25211197338991	22.078738799421906
56-57	22.106772080524557	27.73450774320943	28.12607090279598	22.032649273470035
58-59	21.98917490817756	27.881388547861256	28.231014064458794	21.89842247950239
60-61	22.13799522322381	27.89544803899391	28.14069862859052	21.82585810919176
62-63	22.026691438972094	28.011264197717562	28.088493300135564	21.87355106317478
64-65	21.95388200272289	27.956870271422428	28.10274646889385	21.986501256960832
66-67	22.056201720514302	27.955739640610965	27.97087459601472	22.017184042860013
68-69	22.026581960037404	27.89450267966027	28.055786502082427	22.023128858219902
70-71	22.036580200236557	27.610281274648056	28.168136973062612	22.185001552052775
72-73	22.110816272038345	27.60248298624023	28.09798556613493	22.188715175586495
74-75	21.96999501791281	27.764737502198823	28.05429419875755	22.210973281130816
76-77	22.10708792724242	27.710577341675258	27.986785919033714	22.195548812048607
78-79	22.08529098333249	27.707443220776725	27.962325012959933	22.24494078293085
80-81	22.095987287835523	27.753149867413008	27.953026726554125	22.197836118197344
82-83	22.152332261637962	27.704320676715287	28.00622058159119	22.137126480055556
84-85	22.241937806519267	27.706282166180458	27.936972274821485	22.11480775247879
86-87	22.095756757186955	27.76486977121398	27.968781118815688	22.170592352783373
88-89	22.154449687453763	27.691234654778384	27.95047318562464	22.203842472143215
90-91	22.17493722871209	27.73583045209272	27.961582289527176	22.12765002966802
92-93	22.243457486913616	27.739921099876142	27.87519696035749	22.14142445285275
94-95	22.077093114171433	27.84628929756796	27.901345646880355	22.175271941380252
96-97	22.11396461054217	27.835501457932377	27.93025097034572	22.120282961179736
98-99	22.37647767313634	28.36812331370981	27.990501716179033	21.264897296974823
100-101	22.979606355932958	30.890833044817796	25.34982038794304	20.779740211306205
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2853.0
1	2834.0
2	3662.5
3	4774.0
4	5948.0
5	8339.5
6	10602.0
7	13401.0
8	16253.0
9	17660.0
10	17502.5
11	16869.0
12	18145.0
13	22561.0
14	32181.0
15	44178.5
16	53155.5
17	57675.0
18	57801.0
19	54449.5
20	49987.5
21	46049.5
22	45404.0
23	51181.0
24	62682.0
25	80598.0
26	104600.5
27	131076.5
28	158799.5
29	187092.0
30	214680.0
31	243674.0
32	274468.5
33	307222.5
34	339504.0
35	368035.5
36	399804.0
37	428293.5
38	449092.5
39	466930.0
40	484132.5
41	500935.5
42	511990.5
43	520863.5
44	535595.0
45	546105.0
46	543813.0
47	541252.0
48	539010.0
49	530652.5
50	516942.0
51	498230.5
52	475120.0
53	448983.5
54	420443.0
55	388410.0
56	354731.5
57	317520.5
58	279277.0
59	241053.5
60	199837.5
61	160769.0
62	126331.0
63	96763.5
64	71991.0
65	52625.0
66	38193.0
67	28226.5
68	20922.5
69	15143.0
70	10923.5
71	7742.5
72	5314.0
73	3438.5
74	2132.5
75	1318.0
76	847.5
77	502.0
78	305.0
79	212.0
80	148.5
81	98.5
82	67.5
83	49.5
84	40.5
85	35.0
86	28.0
87	19.5
88	11.0
89	6.5
90	3.5
91	1.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.003007287193051752
4	0.0
5	0.05107725767423557
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	7.770767940702203E-6
16-17	3.885383970351101E-6
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	7.92887241756334E-4
28-29	1.8663025199399844E-4
30-31	0.0
32-33	0.0
34-35	7.035370638568543E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	9.734986490880935E-5
48-49	0.0
50-51	2.0611160481710853E-5
52-53	2.0760281363262903E-5
54-55	0.001137542096899098
56-57	0.002094868545734247
58-59	0.008465882980332504
60-61	0.007654119510547771
62-63	0.004298962373061795
64-65	0.005070364152769375
66-67	0.006509848904692647
68-69	0.003661574149339765
70-71	0.005375736641810049
72-73	9.91438992209055E-4
74-75	0.0016398092705406666
76-77	0.0014984549546228649
78-79	0.0011362514505833068
80-81	7.058126020487388E-5
82-83	0.0
84-85	0.0
86-87	2.7661092988781583E-4
88-89	9.909856608299574E-4
90-91	0.0
92-93	5.0208163043980345E-6
94-95	1.320903563066236E-4
96-97	3.4501062117772E-4
98-99	1.0634231410127267E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	145.0
20-21	566.0
22-23	1311.0
24-25	1681.0
26-27	3723.0
28-29	8110.0
30-31	22493.0
32-33	29398.0
34-35	40493.0
36-37	64157.0
38-39	76046.0
40-41	76203.0
42-43	86827.0
44-45	104080.0
46-47	104911.0
48-49	96862.0
50-51	88648.0
52-53	85008.0
54-55	91691.0
56-57	97193.0
58-59	97555.0
60-61	96034.0
62-63	92827.0
64-65	101612.0
66-67	108386.0
68-69	110295.0
70-71	113400.0
72-73	98180.0
74-75	100258.0
76-77	106186.0
78-79	110273.0
80-81	111541.0
82-83	107040.0
84-85	105375.0
86-87	109860.0
88-89	115214.0
90-91	118018.0
92-93	115657.0
94-95	123845.0
96-97	198450.0
98-99	1073514.0
100-101	8475675.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.27437382822363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.36065340365894	75.30813110474278
2	7.407710410653522	12.485603127172777
3	1.746677805148797	4.416005349257126
4	0.6756272489082397	2.2775225337210916
5	0.3043837376681186	1.2825874447737493
6	0.164338375762099	0.830970822797893
7	0.0918446712171709	0.5418106509400306
8	0.06206249522879814	0.4184222338899264
9	0.04503334005507458	0.3415640881081385
>10	0.13819164566398584	1.7979827854206487
>50	0.002565967517260519	0.1434158004034884
>100	8.636359281487987E-4	0.11324620911139104
>500	1.575423506819848E-5	0.012174809969873183
>1k	3.150835477177037E-5	0.030563039691139028
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.3312303822106606E-5	0.0	0.0	0.0
3	0.0	2.3312303822106606E-5	0.0	0.0	0.0
4	0.0	8.547844734772422E-5	0.0	1.5541535881404405E-5	0.0
5	0.0	8.547844734772422E-5	0.0	1.5541535881404405E-5	0.0
6	7.770767940702203E-6	8.547844734772422E-5	0.0	3.108307176280881E-5	0.0
7	7.770767940702203E-6	8.547844734772422E-5	0.0	3.885383970351101E-5	0.0
8	7.770767940702203E-6	8.547844734772422E-5	0.0	3.885383970351101E-5	0.0
9	7.770767940702203E-6	1.3210305499193744E-4	0.0	7.770767940702202E-5	0.0
10-11	1.5541535881404405E-5	1.3210305499193744E-4	0.0	9.713459925877752E-5	0.0
12-13	2.3312303822106606E-5	1.3210305499193744E-4	0.0	1.2044690308088414E-4	3.885383970351101E-6
14-15	3.108307176280881E-5	1.5541535881404404E-4	0.0	1.2433228705123524E-4	7.770767940702203E-6
16-17	3.885383970351101E-5	1.6707151072509733E-4	0.0	1.4375920690299076E-4	7.770767940702203E-6
18-19	3.885383970351101E-5	1.8261304660650173E-4	0.0	1.7872766263615065E-4	1.1656151911053305E-5
20-21	3.885383970351101E-5	2.0203996645825725E-4	0.0	2.1758150233966168E-4	1.5541535881404405E-5
22-23	6.605152749596872E-5	2.0981073439895945E-4	0.0	2.603207260135238E-4	1.5541535881404405E-5
24-25	7.770767940702202E-5	2.1758150233966168E-4	0.0	2.991745657170348E-4	1.5541535881404405E-5
26-27	7.770767940702202E-5	2.2535227028036388E-4	0.0	4.196214687979189E-4	1.5541535881404405E-5
28-29	9.324921528842642E-5	2.2535227028036388E-4	0.0	7.071398826039004E-4	1.5541535881404405E-5
30-31	1.1656151911053304E-4	2.642061099838749E-4	0.0	0.001647402803428867	1.5541535881404405E-5
32-33	1.2433228705123524E-4	2.642061099838749E-4	0.0	0.0036600317000707377	1.5541535881404405E-5
34-35	1.2433228705123524E-4	3.186014855687903E-4	0.0	0.00663235043738933	1.5541535881404405E-5
36-37	1.2433228705123524E-4	3.84653013064759E-4	0.0	0.011446341176654343	1.5541535881404405E-5
38-39	1.2433228705123524E-4	4.0796531688686564E-4	0.0	0.018280731580501933	1.5541535881404405E-5
40-41	1.2821767102158635E-4	4.6236069247178107E-4	0.0	0.025655190356228322	1.5541535881404405E-5
42-43	1.3598843896228853E-4	4.8178761232353656E-4	0.0	0.032718818414326625	1.5541535881404405E-5
44-45	1.3987382293263964E-4	5.40068371878803E-4	0.0	0.04102188395896693	1.5541535881404405E-5
46-47	1.3987382293263964E-4	5.75036827611963E-4	0.0	0.049845590955634275	1.5541535881404405E-5
48-49	1.4375920690299076E-4	5.828075955526652E-4	0.0	0.05894516021419655	1.5541535881404405E-5
50-51	1.5930074278439516E-4	6.100052833451229E-4	0.0	0.0681107810002548	3.108307176280881E-5
52-53	1.6318612675474625E-4	6.13890667315474E-4	0.0	0.07716761103514322	3.108307176280881E-5
54-55	1.6707151072509733E-4	6.13890667315474E-4	0.0	0.08625552414179445	3.108307176280881E-5
56-57	1.7095689469544845E-4	6.255468192265273E-4	0.0	0.09282182305168782	3.885383970351101E-5
58-59	1.9815458248790614E-4	6.294322031968784E-4	0.0	0.09815256985900953	4.662460764421321E-5
60-61	2.1369611836931056E-4	6.372029711375806E-4	0.0	0.10361930510529352	4.662460764421321E-5
62-63	2.1758150233966168E-4	6.605152749596872E-4	0.0	0.1090161034401112	4.662460764421321E-5
64-65	2.1758150233966168E-4	7.226814184853049E-4	0.0	0.11436627716728466	4.662460764421321E-5
66-67	2.2146688631001276E-4	7.343375703963581E-4	0.0	0.11963874321505111	4.662460764421321E-5
68-69	2.2535227028036388E-4	7.459937223074114E-4	0.0	0.12515987383692	4.662460764421321E-5
70-71	2.2535227028036388E-4	7.459937223074114E-4	0.0	0.13012927993499906	4.662460764421321E-5
72-73	2.4089380616176828E-4	7.498791062777626E-4	0.0	0.13421670387180845	4.662460764421321E-5
74-75	2.4089380616176828E-4	7.69306026129518E-4	0.0	0.13757756100616214	5.439537558491542E-5
76-77	2.4089380616176828E-4	7.809621780405713E-4	0.0	0.14077134662979074	5.439537558491542E-5
78-79	2.4089380616176828E-4	7.926183299516246E-4	0.0	0.14363487461593952	5.439537558491542E-5
80-81	2.4089380616176828E-4	7.926183299516246E-4	0.0	0.14476163596734132	5.828075955526652E-5
82-83	2.4089380616176828E-4	7.926183299516246E-4	0.0	0.14533278741098293	6.216614352561762E-5
84-85	2.4089380616176828E-4	7.926183299516246E-4	0.0	0.1458223457912472	6.216614352561762E-5
86-87	2.4089380616176828E-4	7.926183299516246E-4	0.0	0.14603215652564616	6.605152749596872E-5
88-89	2.486645741024705E-4	8.431283215661889E-4	0.0	0.1461137495890235	6.993691146631982E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17010	0.0	50.712994	1
GTATCAA	30675	0.0	35.14488	1
TCAACGC	37165	0.0	29.657505	4
ATCAACG	37645	0.0	29.268568	3
CAACGCA	38430	0.0	28.681273	5
TATCAAC	38835	0.0	28.460646	2
AACGCAG	39690	0.0	27.831535	6
ACGCAGA	45670	0.0	24.187292	7
CGCAGAG	46435	0.0	23.863155	8
TGGTATC	7850	0.0	22.494778	2
GTGGTAT	8155	0.0	22.12995	1
GCAGAGT	54155	0.0	20.437468	9
GAGTACT	33050	0.0	19.890665	12-13
CAGAGTA	53310	0.0	18.733839	10-11
CTTATAC	8945	0.0	18.630953	1
AGAGTAC	49680	0.0	17.953007	10-11
GTACTTT	36340	0.0	17.929937	14-15
TACATGG	36030	0.0	17.495111	2
GTACATG	35525	0.0	16.978151	1
ACATGGG	36185	0.0	16.823587	3
>>END_MODULE