FastQCFastQC Report
Fri 27 May 2016
SRR2139699_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139699_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9648946
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121500.048.377291
GTATCAA219000.034.2414131
TCAACGC281600.028.415124
ATCAACG284050.028.1254963
CAACGCA287550.027.7972495
TATCAAC296500.027.1765382
AACGCAG298650.026.7968736
ACGCAGA340000.023.4367717
CGCAGAG345950.023.1330728
TGGTATC59250.020.2472082
GCAGAGT399250.020.0555659
GTGGTAT59700.019.2303071
AGAGTAC368350.019.19429610-11
GAGTACT247800.018.41135212-13
CAGAGTA393850.018.02794310-11
AGTACTT270550.017.2841612-13
GTACTTT275150.016.5817314-15
TACATGG262500.015.9052032
GTACATG264850.015.7803121
ACATGGG263350.015.282413
Produced by FastQC (version 0.11.5)