##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139699_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9648946 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.339013504687454 25.0 18.0 32.0 18.0 33.0 2 29.549715170962713 32.0 27.0 33.0 25.0 33.0 3 30.214220910760616 32.0 30.0 33.0 25.0 33.0 4 30.169996805868745 32.0 31.0 33.0 25.0 33.0 5 30.323206907780392 32.0 31.0 33.0 25.0 33.0 6 32.906881331909204 36.0 31.0 38.0 16.0 38.0 7 34.0277432374479 37.0 34.0 38.0 26.0 38.0 8 34.19228887797693 37.0 34.0 38.0 26.0 38.0 9 35.71283765086881 38.0 36.0 38.0 31.0 38.0 10-11 36.04699373382336 38.0 37.0 38.0 32.0 38.0 12-13 36.22656552332245 38.0 37.0 38.0 33.0 38.0 14-15 36.20585973846262 38.0 37.0 38.0 33.0 38.0 16-17 36.28112453940565 38.0 37.0 38.0 33.0 38.0 18-19 36.387684261058155 38.0 37.0 38.0 33.5 38.0 20-21 36.457970450748036 38.0 38.0 38.0 34.0 38.0 22-23 36.50384167320862 38.0 38.0 38.0 34.0 38.0 24-25 36.4978530983727 38.0 38.0 38.0 34.0 38.0 26-27 36.466902670656566 38.0 38.0 38.0 34.0 38.0 28-29 36.46007570797735 38.0 38.0 38.0 34.0 38.0 30-31 36.46571128405941 38.0 38.0 38.0 34.0 38.0 32-33 36.45101679254921 38.0 38.0 38.0 34.0 38.0 34-35 36.40514045703266 38.0 38.0 38.0 34.0 38.0 36-37 36.38514870046821 38.0 38.0 38.0 34.0 38.0 38-39 36.37628907587242 38.0 38.0 38.0 33.5 38.0 40-41 36.373080295045135 38.0 38.0 38.0 33.5 38.0 42-43 36.33554147635105 38.0 38.0 38.0 33.0 38.0 44-45 36.286823576978115 38.0 37.5 38.0 33.0 38.0 46-47 36.250185840650055 38.0 37.0 38.0 33.0 38.0 48-49 36.20464776860317 38.0 37.0 38.0 33.0 38.0 50-51 36.18291172339021 38.0 37.0 38.0 33.0 38.0 52-53 36.15832217712608 38.0 37.0 38.0 33.0 38.0 54-55 36.112792326325376 38.0 37.0 38.0 33.0 38.0 56-57 36.10831124199579 38.0 37.0 38.0 33.0 38.0 58-59 36.08187462770313 38.0 37.0 38.0 33.0 38.0 60-61 36.06641422160335 38.0 37.0 38.0 33.0 38.0 62-63 36.05984859242217 38.0 37.0 38.0 33.0 38.0 64-65 36.044254830531514 38.0 37.0 38.0 32.0 38.0 66-67 36.04239666728525 38.0 37.0 38.0 32.0 38.0 68-69 36.02842837369278 38.0 37.0 38.0 31.5 38.0 70-71 36.001554345436475 38.0 37.0 38.0 31.0 38.0 72-73 35.96992671090026 38.0 37.0 38.0 31.0 38.0 74-75 35.98173829319674 38.0 37.0 38.0 31.0 38.0 76-77 35.95634955914012 38.0 37.0 38.0 31.0 38.0 78-79 35.93711007634103 38.0 37.0 38.0 31.0 38.0 80-81 35.89514105023816 38.0 37.0 38.0 31.0 38.0 82-83 35.893651076240154 38.0 37.0 38.0 31.0 38.0 84-85 35.88458847567871 38.0 37.0 38.0 31.0 38.0 86-87 35.877953798146784 38.0 37.0 38.0 31.0 38.0 88-89 35.84865966908687 38.0 37.0 38.0 31.0 38.0 90-91 35.83456340943832 38.0 37.0 38.0 31.0 38.0 92-93 35.78361041688453 38.0 37.0 38.0 31.0 38.0 94-95 35.770467564871666 38.0 37.0 38.0 31.0 38.0 96-97 35.79176023733335 38.0 37.0 38.0 31.0 38.0 98-99 35.81924984533417 38.0 37.0 38.0 31.0 38.0 100-101 35.285218649083546 38.0 35.5 38.0 29.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 15.0 19 37.0 20 143.0 21 871.0 22 3662.0 23 10112.0 24 21531.0 25 39182.0 26 62578.0 27 92325.0 28 128443.0 29 168815.0 30 216211.0 31 272961.0 32 349413.0 33 458383.0 34 637426.0 35 998840.0 36 2134571.0 37 4053426.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.0674268464141 19.58448104072714 18.621536486990394 24.72655562586836 2 13.75156416047929 22.146004340784994 40.98688084688214 23.11555065185358 3 18.014130253748213 25.19817520755993 31.991834305679163 24.79586023301269 4 12.243492708944585 18.313502842693907 38.88452686956689 30.55847757879462 5 13.471779801765681 36.8771427913766 35.47864254056127 14.172434866296454 6 29.19680553710219 38.7049424880189 18.61004300366071 13.488208971218205 7 25.6915211257271 32.788037159706356 23.297041977434635 18.22339973713191 8 23.591581919931983 37.52098933914647 21.179815909426793 17.707612831494757 9 24.819995883488208 17.267222761947263 21.555224788282576 36.35755656628196 10-11 23.85273479611141 27.200934692763333 28.88179185581513 20.064538655310123 12-13 24.61133578734921 24.6205129555083 29.12558532299797 21.642565934144518 14-15 21.739919662723768 26.023610559309308 26.866339953935896 25.37012982403103 16-17 21.299539934182445 29.08553064166442 27.611255551189508 22.00367387296363 18-19 21.329314103322787 28.092907764226272 29.537790967013393 21.03998716543755 20-21 22.089730129727187 27.264193866330316 29.456435108942024 21.189640895000473 22-23 21.78873335712753 27.181362708782014 29.355494652965536 21.674409281124923 24-25 21.596588209768996 27.36387871480057 29.45315552345274 21.58637755197769 26-27 21.574279290649173 27.50285783989036 29.35068830511937 21.572174564341097 28-29 21.440129500187524 27.511005690474054 29.40536703474691 21.64349777459151 30-31 21.82614755202951 27.39374983518374 29.343570317832196 21.436532294954556 32-33 21.638489251577138 27.498829434282662 29.27948344356323 21.58319787057697 34-35 21.72012559802217 27.550193333052007 29.27490494786457 21.454776121061244 36-37 21.6955344660498 27.406684697748947 29.322237121562644 21.575543714638616 38-39 21.462972935298872 27.557053360995827 29.369088061821476 21.61088564188383 40-41 21.737240923997234 27.397098770739785 29.276830104709184 21.5888302005538 42-43 21.726363717782533 27.42877595594933 29.11792551168405 21.726934814584084 44-45 21.682660448791523 27.646668197604633 28.943675400402334 21.72699595320151 46-47 21.84005344055618 27.534261556379093 28.65386010494366 21.971824898121067 48-49 21.73299289613846 27.6990006030919 28.644383312236805 21.92362318853284 50-51 21.68506171556721 27.80608528910368 28.57027485380989 21.938578141519216 52-53 21.870292947104705 27.718282873781945 28.499287051744425 21.912137127368922 54-55 21.809581509942465 27.67536380402146 28.48161748554512 22.03343720049096 56-57 21.896977970105347 27.76982716202418 28.406508633149446 21.92668623472103 58-59 21.712336648245557 27.848538867162404 28.571311565062768 21.86781291952927 60-61 21.84043325772777 27.86483698913184 28.486162117139287 21.808567636001097 62-63 21.71769410377788 28.048811879269497 28.423647129632553 21.80984688732007 64-65 21.656021933740803 28.003248405915386 28.41805607292345 21.92267358742036 66-67 21.716852943156365 28.13885843224324 28.161138228437583 21.98315039616281 68-69 21.74613304124052 28.06836513830554 28.237164864218666 21.948336956235277 70-71 21.721876882781412 27.721661621864733 28.355202158862557 22.201259336491294 72-73 21.79022057205901 27.712142973328312 28.319037142399345 22.178599312213336 74-75 21.73525035846593 27.815411242523925 28.269172800594117 22.180165598416025 76-77 21.897296694997618 27.79273010901122 28.179667333155876 22.130305862835282 78-79 21.933994954155068 27.833646738814977 28.055196809969196 22.17716149706076 80-81 21.945556756469685 27.804033153659862 28.029441117606723 22.22096897226373 82-83 21.89983921397247 27.828445309423312 28.101656291649675 22.17005918495455 84-85 22.033695360707295 27.85481472640764 28.058335645410537 22.053154267474525 86-87 21.915866320795573 27.864315181889786 28.08644453470272 22.133373962611923 88-89 21.959119778781456 27.92618047194187 28.03828571209769 22.076414037178985 90-91 21.985359714724606 27.913610372057036 28.086903762844855 22.014126150373503 92-93 22.055558001130194 27.956900905608684 28.008778643736598 21.97876244952452 94-95 22.006881097437624 28.027050652815653 28.027283413737397 21.93878483600932 96-97 21.998928625860252 27.972030399807252 28.137159497613712 21.89188147671879 98-99 22.203227075073332 28.529924137323864 28.222631830494787 21.044216957108016 100-101 22.710976628532883 31.03805671444797 25.625948262166602 20.62501839485254 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2572.0 1 2638.5 2 3236.0 3 3860.5 4 4741.5 5 6603.5 6 8276.0 7 11311.0 8 13892.5 9 14043.5 10 13500.5 11 12855.5 12 13838.5 13 17479.5 14 25811.5 15 36431.5 16 43756.5 17 46497.5 18 45493.0 19 41957.0 20 37803.0 21 35039.5 22 35360.5 23 39993.0 24 48907.0 25 62550.5 26 81335.5 27 102657.0 28 124240.0 29 146670.0 30 168148.5 31 190076.0 32 214165.5 33 239739.5 34 265120.5 35 287503.5 36 312454.0 37 334281.5 38 348552.0 39 360445.0 40 373788.5 41 385782.5 42 391028.0 43 396791.5 44 403032.0 45 408927.0 46 409804.0 47 404792.5 48 399905.0 49 390128.0 50 376728.0 51 362231.0 52 343665.0 53 322680.0 54 301910.0 55 278764.5 56 253615.5 57 227839.0 58 202193.5 59 176132.0 60 147215.0 61 118128.0 62 92534.5 63 71374.5 64 53702.5 65 39748.5 66 29134.0 67 21528.5 68 16153.0 69 11852.0 70 8676.0 71 6296.0 72 4389.5 73 3012.5 74 2063.5 75 1299.5 76 776.5 77 475.5 78 315.5 79 203.5 80 111.5 81 64.5 82 44.0 83 31.5 84 24.0 85 21.0 86 15.5 87 9.0 88 5.0 89 4.5 90 4.5 91 3.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.00297441813852 4 0.0 5 0.04560083557312892 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.181913133310105E-6 16-17 5.181913133310105E-6 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.449938159931098E-4 28-29 2.0743883976967651E-4 30-31 0.0 32-33 0.0 34-35 8.345792471333638E-5 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 2.1522084887407215E-5 46-47 1.1403129572621021E-4 48-49 0.0 50-51 2.7619771204444087E-5 52-53 2.782562084107836E-5 54-55 9.525921770216613E-4 56-57 0.0023255786386796448 58-59 0.008906316301510541 60-61 0.007967204713969608 62-63 0.004407590611415533 64-65 0.005133711302828004 66-67 0.006760633584436691 68-69 0.003605023832717376 70-71 0.0055796364433954295 72-73 9.050062333063552E-4 74-75 0.0015326026578640509 76-77 0.0013494676845202066 78-79 8.694049336040457E-4 80-81 5.690201170107679E-5 82-83 0.0 84-85 0.0 86-87 2.4152951108747747E-4 88-89 8.252187374298555E-4 90-91 0.0 92-93 0.0 94-95 1.2322621848652053E-4 96-97 4.027184047796287E-4 98-99 1.43503987078901E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 144.0 20-21 541.0 22-23 1174.0 24-25 1495.0 26-27 2942.0 28-29 6244.0 30-31 19090.0 32-33 24365.0 34-35 33216.0 36-37 52907.0 38-39 59436.0 40-41 62650.0 42-43 71457.0 44-45 83879.0 46-47 83404.0 48-49 76726.0 50-51 69770.0 52-53 60832.0 54-55 64127.0 56-57 64435.0 58-59 74390.0 60-61 73698.0 62-63 72170.0 64-65 79172.0 66-67 84777.0 68-69 85737.0 70-71 89185.0 72-73 75825.0 74-75 77766.0 76-77 82218.0 78-79 85076.0 80-81 86063.0 82-83 81889.0 84-85 81671.0 86-87 84988.0 88-89 88640.0 90-91 90431.0 92-93 89539.0 94-95 96509.0 96-97 152152.0 98-99 800068.0 100-101 6278148.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.87217172604505 #Duplication Level Percentage of deduplicated Percentage of total 1 90.27259972571655 76.6163158607754 2 6.694178215586905 11.362988861560831 3 1.6094061821583598 4.097813936073087 4 0.603924559141004 2.0502555557196533 5 0.27948633259571815 1.1860306007573167 6 0.1674013841236415 0.85246314123116 7 0.09299849341148336 0.5525088872158019 8 0.06888793580875056 0.46773349742504366 9 0.0464417322232847 0.3547449605258658 >10 0.1603503000552511 2.1171625592910472 >50 0.003487468936805454 0.18775055952805944 >100 7.740336061987864E-4 0.11365270732603679 >500 4.2425269911270026E-5 0.01929986181017492 >1k 2.1211366233011722E-5 0.021279010760521452 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 7.254678386634146E-5 0.0 1.0363826266620209E-5 0.0 3 0.0 7.254678386634146E-5 0.0 1.0363826266620209E-5 0.0 4 0.0 8.291061013296167E-5 0.0 1.0363826266620209E-5 0.0 5 0.0 1.0363826266620209E-4 0.0 1.0363826266620209E-5 0.0 6 4.1455305066480834E-5 1.0363826266620209E-4 0.0 1.0363826266620209E-5 0.0 7 4.1455305066480834E-5 1.140020889328223E-4 0.0 1.0363826266620209E-5 0.0 8 4.1455305066480834E-5 1.140020889328223E-4 0.0 1.0363826266620209E-5 0.0 9 4.1455305066480834E-5 1.7618504653254356E-4 0.0 1.0363826266620209E-5 1.0363826266620209E-5 10-11 4.1455305066480834E-5 1.7618504653254356E-4 0.0 1.0363826266620209E-5 1.0363826266620209E-5 12-13 4.1455305066480834E-5 1.7618504653254356E-4 0.0 1.0363826266620209E-5 1.5545739399930312E-5 14-15 4.1455305066480834E-5 2.5391374353219516E-4 0.0 1.0363826266620209E-5 2.5909565666550526E-5 16-17 8.291061013296167E-5 2.746413960654355E-4 0.0 1.0363826266620209E-5 3.109147879986063E-5 18-19 8.291061013296167E-5 3.264605273985366E-4 0.0 1.0363826266620209E-5 4.663721819979094E-5 20-21 8.291061013296167E-5 3.627339193317073E-4 0.0 2.072765253324042E-5 5.1819131333101044E-5 22-23 8.291061013296167E-5 4.1973496379811847E-4 0.0 4.1455305066480834E-5 7.254678386634146E-5 24-25 9.327443639958188E-5 4.560083557312892E-4 0.0 5.700104446641115E-5 9.327443639958188E-5 26-27 9.327443639958188E-5 4.8709983453114987E-4 0.0 9.327443639958188E-5 1.140020889328223E-4 28-29 9.327443639958188E-5 4.974636607977701E-4 0.0 5.337370527309408E-4 1.140020889328223E-4 30-31 9.327443639958188E-5 5.596466183974913E-4 0.0 0.0019121259461914284 1.140020889328223E-4 32-33 9.327443639958188E-5 5.596466183974913E-4 0.0 0.00539437157177582 1.140020889328223E-4 34-35 9.327443639958188E-5 6.529210547970732E-4 0.0 0.010016638086688432 1.140020889328223E-4 36-37 9.327443639958188E-5 7.565593174632753E-4 0.0 0.01718322395005631 1.140020889328223E-4 38-39 9.327443639958188E-5 7.669231437298955E-4 0.0 0.03124175428072662 1.1918400206613241E-4 40-41 9.327443639958188E-5 8.031965356630661E-4 0.0 0.07624148793039157 1.2436591519944252E-4 42-43 9.327443639958188E-5 8.187422750629965E-4 0.0 0.09034147356612837 1.2436591519944252E-4 44-45 1.0363826266620209E-4 8.964709720626482E-4 0.0 0.11004828921210669 1.2436591519944252E-4 46-47 1.0882017579951219E-4 9.586539296623693E-4 0.0 0.1318952349821421 1.2436591519944252E-4 48-49 1.140020889328223E-4 9.638358427956794E-4 0.0 0.15188187393731917 1.2436591519944252E-4 50-51 1.140020889328223E-4 0.0010156549741287804 0.0 0.17228824785629437 1.2436591519944252E-4 52-53 1.140020889328223E-4 0.0010260188003954006 0.0 0.2088207354461306 1.2954782833275263E-4 54-55 1.140020889328223E-4 0.0010571102791952613 0.0 0.3001882278126543 1.3472974146606272E-4 56-57 1.140020889328223E-4 0.0011037474973950522 0.0 0.400344244853272 1.3472974146606272E-4 58-59 1.140020889328223E-4 0.0011244751499282927 0.0 0.508475226206054 1.3472974146606272E-4 60-61 1.2436591519944252E-4 0.0011659304549947735 0.0 0.535612905285199 1.3472974146606272E-4 62-63 1.6582122026592334E-4 0.001233295325727805 0.0 0.552138026267325 1.4509356773268292E-4 64-65 1.6582122026592334E-4 0.0013783888934604878 0.0 0.5648751687490012 1.4509356773268292E-4 66-67 1.6582122026592334E-4 0.001383570806593798 0.0 0.572290486442768 1.4509356773268292E-4 68-69 1.6582122026592334E-4 0.0014094803722603485 0.0 0.5791720670838039 1.5027548086599303E-4 70-71 1.6582122026592334E-4 0.001425026111660279 0.0 0.585043174663844 1.5545739399930314E-4 72-73 1.6582122026592334E-4 0.0014457537641935192 0.0 0.5891990689967588 1.6582122026592334E-4 74-75 1.6582122026592334E-4 0.00149239098239331 0.0 0.5929766836709418 1.6582122026592334E-4 76-77 1.6582122026592334E-4 0.0015649377662596516 0.0 0.5960858315509279 1.6582122026592334E-4 78-79 1.6582122026592334E-4 0.0016063930713261325 0.0 0.598827063598449 1.6582122026592334E-4 80-81 1.6582122026592334E-4 0.001637484550125993 0.0 0.6001018142292432 1.8136695966585364E-4 82-83 1.6582122026592334E-4 0.0016478483763926132 0.0 0.6006251874557076 1.9691269906578398E-4 84-85 1.6582122026592334E-4 0.0016582122026592334 0.0 0.6011744702478384 1.9691269906578398E-4 86-87 1.6582122026592334E-4 0.0017203951602589548 0.0 0.6012988361630379 1.9691269906578398E-4 88-89 1.7618504653254356E-4 0.001787760030991986 0.0 0.601350655294371 1.9691269906578398E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12150 0.0 48.37729 1 GTATCAA 21900 0.0 34.241413 1 TCAACGC 28160 0.0 28.41512 4 ATCAACG 28405 0.0 28.125496 3 CAACGCA 28755 0.0 27.797249 5 TATCAAC 29650 0.0 27.176538 2 AACGCAG 29865 0.0 26.796873 6 ACGCAGA 34000 0.0 23.436771 7 CGCAGAG 34595 0.0 23.133072 8 TGGTATC 5925 0.0 20.247208 2 GCAGAGT 39925 0.0 20.055565 9 GTGGTAT 5970 0.0 19.230307 1 AGAGTAC 36835 0.0 19.194296 10-11 GAGTACT 24780 0.0 18.411352 12-13 CAGAGTA 39385 0.0 18.027943 10-11 AGTACTT 27055 0.0 17.28416 12-13 GTACTTT 27515 0.0 16.58173 14-15 TACATGG 26250 0.0 15.905203 2 GTACATG 26485 0.0 15.780312 1 ACATGGG 26335 0.0 15.28241 3 >>END_MODULE