FastQCFastQC Report
Fri 27 May 2016
SRR2139698_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139698_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9506646
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156900.053.2872541
GTATCAA277800.037.0725481
ATCAACG359550.028.6196063
TCAACGC361250.028.4967924
TATCAAC366850.028.23712
CAACGCA371100.027.7288575
AACGCAG384550.026.8541166
GTGGTAT67600.025.7508161
TGGTATC65650.025.2748132
ACGCAGA436700.023.5393077
CGCAGAG441500.023.2820428
CTTATAC112300.022.678711
GCAGAGT508350.020.2372199
TACATGG383200.019.9273762
GTACATG388600.019.8380321
TATACAC147900.019.7419783
GAGTACT278000.019.44303112-13
CAGAGTA494750.018.81029110-11
ACATGGG395100.018.8062933
AGAGTAC471050.018.7015510-11
Produced by FastQC (version 0.11.5)