##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139698_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9506646
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.467243126545366	31.0	25.0	33.0	18.0	33.0
2	31.013107777443274	33.0	31.0	33.0	27.0	33.0
3	31.115200881572743	33.0	31.0	33.0	27.0	34.0
4	31.888904456945173	33.0	32.0	33.0	29.0	34.0
5	32.332757420440394	33.0	33.0	34.0	31.0	34.0
6	35.90494944273722	38.0	36.0	38.0	31.0	38.0
7	36.207519770905535	38.0	37.0	38.0	33.0	38.0
8	36.46246962388207	38.0	37.0	38.0	34.0	38.0
9	36.6783105208714	38.0	38.0	38.0	34.0	38.0
10-11	36.703871533661825	38.0	38.0	38.0	34.0	38.0
12-13	36.747623715030514	38.0	38.0	38.0	34.0	38.0
14-15	36.76288561707252	38.0	38.0	38.0	34.0	38.0
16-17	36.75166257374052	38.0	38.0	38.0	34.0	38.0
18-19	36.758438833212054	38.0	38.0	38.0	34.5	38.0
20-21	36.78622887274929	38.0	38.0	38.0	35.0	38.0
22-23	36.76612754537729	38.0	38.0	38.0	34.5	38.0
24-25	36.769934184902965	38.0	38.0	38.0	34.5	38.0
26-27	36.738705159466974	38.0	38.0	38.0	34.5	38.0
28-29	36.658719339699175	38.0	38.0	38.0	34.0	38.0
30-31	36.687196024881544	38.0	38.0	38.0	34.0	38.0
32-33	36.68429469649949	38.0	38.0	38.0	34.0	38.0
34-35	36.66918234891644	38.0	38.0	38.0	34.0	38.0
36-37	36.641935843785674	38.0	38.0	38.0	34.0	38.0
38-39	36.62226577836417	38.0	38.0	38.0	34.0	38.0
40-41	36.63140160415877	38.0	38.0	38.0	34.0	38.0
42-43	36.607757225825225	38.0	38.0	38.0	34.0	38.0
44-45	36.54205592603164	38.0	38.0	38.0	34.0	38.0
46-47	36.41907888773565	38.0	38.0	38.0	34.0	38.0
48-49	36.36391916262427	38.0	38.0	38.0	33.5	38.0
50-51	36.369799434721685	38.0	38.0	38.0	34.0	38.0
52-53	36.37385097569757	38.0	38.0	38.0	34.0	38.0
54-55	36.297601560978265	38.0	38.0	38.0	33.0	38.0
56-57	36.31354958616578	38.0	38.0	38.0	33.0	38.0
58-59	36.25018628870341	38.0	38.0	38.0	33.0	38.0
60-61	36.2618196249094	38.0	38.0	38.0	33.0	38.0
62-63	36.278503097715	38.0	38.0	38.0	33.0	38.0
64-65	36.26556587323438	38.0	38.0	38.0	33.0	38.0
66-67	36.246934574505886	38.0	37.0	38.0	33.0	38.0
68-69	36.22458793664002	38.0	37.0	38.0	33.0	38.0
70-71	36.224582098989735	38.0	37.0	38.0	33.0	38.0
72-73	36.21497908839541	38.0	37.0	38.0	33.0	38.0
74-75	36.17560753776504	38.0	37.0	38.0	33.0	38.0
76-77	36.16822210805461	38.0	37.0	38.0	33.0	38.0
78-79	36.15629984840574	38.0	37.0	38.0	33.0	38.0
80-81	36.16484601435917	38.0	37.0	38.0	33.0	38.0
82-83	36.13508901259485	38.0	37.0	38.0	33.0	38.0
84-85	36.10046388107264	38.0	37.0	38.0	33.0	38.0
86-87	36.10091044309344	38.0	37.0	38.0	33.0	38.0
88-89	36.10814707366417	38.0	37.0	38.0	33.0	38.0
90-91	36.05779090647259	38.0	37.0	38.0	33.0	38.0
92-93	36.08076007955016	38.0	37.0	38.0	33.0	38.0
94-95	36.03616013228968	38.0	37.0	38.0	32.5	38.0
96-97	36.0611030700143	38.0	37.0	38.0	33.0	38.0
98-99	36.1055073536273	38.0	37.0	38.0	33.0	38.0
100-101	35.568402740049265	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	7.0
18	17.0
19	36.0
20	131.0
21	529.0
22	2139.0
23	6574.0
24	15056.0
25	28232.0
26	46315.0
27	69670.0
28	99363.0
29	133376.0
30	170457.0
31	215558.0
32	270721.0
33	351577.0
34	482760.0
35	751935.0
36	1582800.0
37	5279393.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.041703456718594	20.574995639892347	15.806058203913345	23.577242699475715
2	14.442748788584323	21.783013693788536	41.54936451825386	22.22487299937328
3	17.97984273317845	26.763035038855975	31.068412561065173	24.188709666900397
4	12.654704929582946	18.926075505493735	37.76133033669288	30.657889228230438
5	13.460404581629405	37.69940114138675	35.11982961084189	13.720364666141954
6	29.43351419627911	38.18696941066281	18.610927555312358	13.768588837745721
7	26.17217504766838	33.09520358179237	22.6366467213013	18.09597464923795
8	24.321711358559053	37.02910574349776	20.788025556016283	17.8611573419269
9	25.42626495190838	17.221615278406286	21.065547197192362	36.28657257249297
10-11	24.19416374607827	27.16852505079078	28.308921989942615	20.32838921318833
12-13	25.151200810001807	24.600312486670507	28.63116122295714	21.617325480370546
14-15	22.22693997441369	25.85852570927749	26.616079950804945	25.29845436550388
16-17	21.531132010174776	29.081660345825437	27.253165837878047	22.134041806121736
18-19	21.55154404613362	27.823850809212843	29.38362278347169	21.24098236118185
20-21	22.53441691827328	26.846145612840605	29.298791367822176	21.32064610106394
22-23	22.20607510206297	26.89458091823485	29.19031531875092	21.70902866095126
24-25	21.84771658522292	27.08892563166032	29.40583501630541	21.65752276681135
26-27	21.72324670067823	27.20690755942873	29.374096346967406	21.695749392925638
28-29	21.511638495948866	27.093459068526588	29.446303823062813	21.948598612461737
30-31	21.79591040467682	26.916470505345536	29.50114150264148	21.786477587336165
32-33	21.617397292684164	27.130495538505738	29.430647212411067	21.821459956399032
34-35	21.729155990562727	27.306647504582738	29.467630405939456	21.49656609891508
36-37	21.78465419540266	27.107316482211385	29.259270565178447	21.84875875720751
38-39	21.653165401934118	27.16070976263378	29.41566584736296	21.77045898806914
40-41	21.86640266037004	27.010491676003394	29.3807104215132	21.742395242113364
42-43	21.93078373418892	27.014168302134046	29.225171604088857	21.829876359588177
44-45	21.854220399891396	27.333170555783255	28.961872047549274	21.850736996776075
46-47	22.086270439192454	27.16371506737376	28.544781949739534	22.205232543694255
48-49	21.982581164626783	27.2774240263246	28.57222475403869	22.167770055009928
50-51	21.8324288360451	27.41914050572401	28.57655114014776	22.171879518083127
52-53	21.98690404675095	27.40747538242319	28.588898150484788	22.01672242034107
54-55	21.97644674207586	27.442173566826284	28.372855794009244	22.20852389708861
56-57	22.08525553976419	27.605356080564903	28.183459934197646	22.125928445473264
58-59	21.880015352903744	27.787868829251817	28.406657400277574	21.925458417566865
60-61	22.025163378340405	27.90739179720999	28.16195529466871	21.905489529780894
62-63	21.839518606431948	28.07099787090624	28.154768298759524	21.93471522390229
64-65	21.80052867993121	28.024636106341273	28.143652463905184	22.03118274982233
66-67	21.91830310810852	28.03658334546677	27.986749627118435	22.058363919306274
68-69	21.951971419419795	27.902821460165733	28.066552220935893	22.078654899478583
70-71	21.95553541166145	27.504894972410437	28.231832085598878	22.307737530329234
72-73	22.05565114439304	27.442260647755162	28.22269274655509	22.2793954612967
74-75	21.926215575597784	27.53697759270835	28.18811284013166	22.348693991562214
76-77	22.077108521855834	27.4875830666442	28.11223069121862	22.323077720281344
78-79	22.074773418918415	27.544195008798344	28.02268342162363	22.35834815065961
80-81	22.03961619609126	27.59317984907359	27.99677111480085	22.370432840034297
82-83	22.109750745204177	27.536776903764814	28.041229841606558	22.312242509424447
84-85	22.226757112542874	27.574158269549205	27.933684520812147	22.265400097095778
86-87	22.061801385736736	27.61646519163196	28.00327485152368	22.31845857110762
88-89	22.171893622065276	27.5734539547586	27.975033166576722	22.2796192565994
90-91	22.1521232480676	27.54293689127657	28.059065052108757	22.245874808547075
92-93	22.15237142386503	27.57718033790082	27.981699765865585	22.288748472368564
94-95	22.046514234688054	27.60290255712076	28.01639751757668	22.334185690614504
96-97	22.06458830702172	27.652976454696464	28.075391167048263	22.20704407123355
98-99	22.32145920953752	28.263320772989065	28.071078071462193	21.344141946011224
100-101	22.928848735543955	30.750891378867035	25.40995745344422	20.91030243214479
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3942.0
1	3691.5
2	3683.5
3	4174.0
4	5171.0
5	7338.5
6	9542.5
7	12660.0
8	16061.5
9	17306.0
10	16268.5
11	15018.5
12	16047.0
13	19868.0
14	28568.5
15	39593.0
16	47572.5
17	50983.0
18	49997.0
19	45646.5
20	40135.5
21	35184.5
22	33232.5
23	36113.5
24	43421.0
25	55675.5
26	72750.5
27	91324.0
28	110027.0
29	129962.5
30	149744.0
31	171289.5
32	195972.0
33	221505.5
34	246479.5
35	269645.0
36	294181.0
37	315442.5
38	329687.0
39	342465.5
40	357674.0
41	370356.0
42	376992.0
43	386452.5
44	406663.0
45	418449.0
46	413868.5
47	408768.5
48	401101.0
49	391802.5
50	381980.5
51	369267.0
52	352323.0
53	332210.5
54	312049.0
55	289864.5
56	265294.5
57	238358.0
58	211464.0
59	185542.0
60	157286.0
61	127737.0
62	99492.0
63	75532.5
64	56953.0
65	42480.5
66	30757.0
67	22614.0
68	16858.0
69	11897.0
70	8474.0
71	6046.5
72	4157.0
73	2771.0
74	1860.0
75	1193.0
76	745.0
77	458.0
78	316.0
79	217.0
80	133.0
81	85.0
82	61.5
83	47.5
84	38.0
85	26.0
86	13.0
87	4.0
88	1.5
89	1.5
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.05861162811784514
6	0.0
7	0.0036185211903335835
8	0.0
9	0.0
10-11	0.0
12-13	5.259478474322069E-6
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.209306724551649E-5
28-29	5.263881817009887E-6
30-31	1.580918857442539E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	2.7342246176460293E-5
46-47	5.521307803468607E-6
48-49	0.0
50-51	0.0
52-53	8.213580276984585E-4
54-55	1.8829059583760243E-4
56-57	0.0028751404146700014
58-59	0.007587760947388471
60-61	0.003379875228806735
62-63	0.00578059810668896
64-65	0.0068545295677451545
66-67	0.005281746632150443
68-69	0.0039171409899289395
70-71	0.005381062456382527
72-73	0.003408670540257517
74-75	0.0028135610383581734
76-77	0.0024358430573031766
78-79	5.753056934744417E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	7.335533993531393E-5
88-89	0.0
90-91	1.500786753346652E-4
92-93	0.0
94-95	1.32776640479852E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	139.0
20-21	596.0
22-23	1158.0
24-25	1472.0
26-27	3117.0
28-29	7424.0
30-31	19408.0
32-33	24966.0
34-35	34155.0
36-37	53234.0
38-39	61571.0
40-41	62704.0
42-43	72221.0
44-45	86554.0
46-47	86423.0
48-49	78667.0
50-51	70658.0
52-53	62674.0
54-55	66928.0
56-57	68604.0
58-59	73762.0
60-61	75554.0
62-63	72382.0
64-65	80929.0
66-67	87255.0
68-69	86998.0
70-71	95582.0
72-73	73955.0
74-75	75214.0
76-77	79711.0
78-79	83023.0
80-81	84067.0
82-83	78919.0
84-85	78832.0
86-87	81151.0
88-89	85507.0
90-91	87366.0
92-93	86376.0
94-95	92727.0
96-97	144316.0
98-99	782379.0
100-101	6157968.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.00597115767381
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.64682166646212	76.20515136956406
2	7.217599635979674	12.270781329674502
3	1.7818246440662309	4.543972029045793
4	0.6363770157753874	2.1638338499363647
5	0.2944665569820699	1.2515707824858675
6	0.1398052890418637	0.713057062078975
7	0.07852584307276968	0.4672615885963115
8	0.05410585558525318	0.36794566394730377
9	0.03182705849901247	0.2434941015120586
>10	0.11563941971414252	1.507863686581481
>50	0.0024450894176616823	0.13734991682716027
>100	4.811414453079477E-4	0.06964091819864271
>500	6.0588834084430535E-5	0.03598369334771231
>1k	2.0195124304257313E-5	0.022094008203696013
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.1556870845932415E-5	0.0	0.0	1.0518956948644138E-5
5	0.0	3.1556870845932415E-5	0.0	0.0	1.0518956948644138E-5
6	0.0	3.1556870845932415E-5	0.0	0.0	2.1037913897288275E-5
7	0.0	4.207582779457655E-5	0.0	0.0	2.1037913897288275E-5
8	0.0	4.207582779457655E-5	0.0	0.0	3.1556870845932415E-5
9	0.0	1.5778435422966207E-4	0.0	1.0518956948644138E-5	3.1556870845932415E-5
10-11	0.0	1.5778435422966207E-4	0.0	1.5778435422966207E-5	3.1556870845932415E-5
12-13	0.0	1.5778435422966207E-4	0.0	2.1037913897288275E-5	3.1556870845932415E-5
14-15	0.0	2.0511966049856068E-4	0.0	2.6297392371610344E-5	3.681634932025448E-5
16-17	0.0	2.3141705287017103E-4	0.0	3.1556870845932415E-5	4.207582779457655E-5
18-19	0.0	2.5771444524178136E-4	0.0	3.681634932025448E-5	4.207582779457655E-5
20-21	0.0	2.840118376133917E-4	0.0	4.207582779457655E-5	4.207582779457655E-5
22-23	0.0	3.260876654079683E-4	0.0	5.785426321754276E-5	5.7854263217542765E-5
24-25	0.0	3.4712557930525654E-4	0.0	6.311374169186483E-5	8.41516555891531E-5
26-27	0.0	3.5764453625390067E-4	0.0	1.1044904796076345E-4	8.41516555891531E-5
28-29	0.0	3.5764453625390067E-4	0.0	5.469857613294952E-4	8.41516555891531E-5
30-31	0.0	3.9972036404847724E-4	0.0	0.002130088782100438	8.41516555891531E-5
32-33	0.0	3.9972036404847724E-4	0.0	0.0056276419675246134	8.41516555891531E-5
34-35	1.0518956948644138E-5	4.365367133687317E-4	0.0	0.010240204589505069	9.467061253779724E-5
36-37	1.0518956948644138E-5	4.733530626889862E-4	0.0	0.01728264626662232	9.467061253779724E-5
38-39	1.0518956948644138E-5	4.733530626889862E-4	0.0	0.02966871807365079	9.993009101211931E-5
40-41	1.0518956948644138E-5	5.049099335349186E-4	0.0	0.06256675593053533	1.1570852643508552E-4
42-43	1.0518956948644138E-5	5.31207325906529E-4	0.0	0.07455836685198966	1.209680049094076E-4
44-45	1.0518956948644138E-5	5.838021106497497E-4	0.0	0.090420953930545	1.3148696185805172E-4
46-47	1.0518956948644138E-5	6.1009950302136E-4	0.0	0.1080770231688442	1.367464403323738E-4
48-49	1.0518956948644138E-5	6.311374169186483E-4	0.0	0.1245234123580493	1.367464403323738E-4
50-51	1.0518956948644138E-5	6.995106370848352E-4	0.0	0.14126959182029075	1.367464403323738E-4
52-53	1.0518956948644138E-5	7.047701155591573E-4	0.0	0.16463219520322941	1.4200591880669588E-4
54-55	1.0518956948644138E-5	7.152890725078014E-4	0.0	0.2188994941012845	1.4726539728101795E-4
56-57	1.0518956948644138E-5	7.258080294564455E-4	0.0	0.2775689764823472	1.4726539728101795E-4
58-59	1.0518956948644138E-5	7.258080294564455E-4	0.0	0.3423236754582005	1.4726539728101795E-4
60-61	2.1037913897288275E-5	7.468459433537338E-4	0.0	0.3667697314068495	1.4726539728101795E-4
62-63	2.1037913897288275E-5	7.57364900302378E-4	0.0	0.3793819607882738	1.4726539728101795E-4
64-65	2.1037913897288275E-5	8.520355128401752E-4	0.0	0.3913946096236254	1.4726539728101795E-4
66-67	2.1037913897288275E-5	8.730734267374634E-4	0.0	0.397790135448401	1.4726539728101795E-4
68-69	2.1037913897288275E-5	9.046302975833959E-4	0.0	0.4041804017947024	1.4726539728101795E-4
70-71	2.1037913897288275E-5	9.256682114806842E-4	0.0	0.4100710176859431	1.4726539728101795E-4
72-73	2.1037913897288275E-5	9.414466469036503E-4	0.0	0.41465202343707763	1.683033111783062E-4
74-75	2.1037913897288275E-5	9.83522474698227E-4	0.0	0.41835469628300037	1.683033111783062E-4
76-77	2.1037913897288275E-5	0.0010203388240184815	0.0	0.4215629781523368	1.683033111783062E-4
78-79	2.1037913897288275E-5	0.0010361172594414475	0.0	0.42470814627998144	1.7356278965262827E-4
80-81	2.1037913897288275E-5	0.0010413767379157696	0.0	0.42607561068330513	1.840817466012724E-4
82-83	2.1037913897288275E-5	0.0010571551733387359	0.0	0.4265016284397252	1.893412250755945E-4
84-85	2.1037913897288275E-5	0.0010729336087617022	0.0	0.42691712723919667	1.9460070354991655E-4
86-87	2.1037913897288275E-5	0.0010729336087617022	0.0	0.42715380377054113	1.9986018202423862E-4
88-89	2.1037913897288275E-5	0.0011360473504535668	0.0	0.4271958795983357	2.1037913897288275E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15690	0.0	53.287254	1
GTATCAA	27780	0.0	37.072548	1
ATCAACG	35955	0.0	28.619606	3
TCAACGC	36125	0.0	28.496792	4
TATCAAC	36685	0.0	28.2371	2
CAACGCA	37110	0.0	27.728857	5
AACGCAG	38455	0.0	26.854116	6
GTGGTAT	6760	0.0	25.750816	1
TGGTATC	6565	0.0	25.274813	2
ACGCAGA	43670	0.0	23.539307	7
CGCAGAG	44150	0.0	23.282042	8
CTTATAC	11230	0.0	22.67871	1
GCAGAGT	50835	0.0	20.237219	9
TACATGG	38320	0.0	19.927376	2
GTACATG	38860	0.0	19.838032	1
TATACAC	14790	0.0	19.741978	3
GAGTACT	27800	0.0	19.443031	12-13
CAGAGTA	49475	0.0	18.810291	10-11
ACATGGG	39510	0.0	18.806293	3
AGAGTAC	47105	0.0	18.70155	10-11
>>END_MODULE