FastQCFastQC Report
Fri 27 May 2016
SRR2139697_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139697_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10888869
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160350.046.399881
GTATCAA283500.036.264391
TCAACGC345150.029.537134
ATCAACG346550.029.4178073
CAACGCA352650.028.847835
AACGCAG365150.027.9032966
TATCAAC368750.027.8688642
CTTATAC83050.026.471321
ACGCAGA413300.024.6421037
CGCAGAG418350.024.3332258
GCAGAGT487700.020.9172539
GTGGTAT87200.020.3175071
TATACAC127150.019.8667263
TGGTATC84000.019.8598842
GAGTACT300400.019.64700512-13
AGAGTAC438350.019.37052210-11
CAGAGTA471500.018.58881610-11
TATGCCG69650.017.89582342-43
GTACATG310550.017.878431
AGTACTT325650.017.80613712-13
Produced by FastQC (version 0.11.5)