##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139697_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10888869
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.263808757364973	31.0	25.0	33.0	18.0	33.0
2	30.975660465747175	33.0	31.0	33.0	27.0	33.0
3	31.10591880570884	33.0	31.0	33.0	27.0	34.0
4	31.892158313227938	33.0	32.0	33.0	29.0	34.0
5	32.345704406949885	33.0	33.0	34.0	31.0	34.0
6	35.931782630500926	38.0	36.0	38.0	31.0	38.0
7	36.240303194023184	38.0	37.0	38.0	33.0	38.0
8	36.49358670767368	38.0	37.0	38.0	34.0	38.0
9	36.69578190352001	38.0	38.0	38.0	34.0	38.0
10-11	36.72413870531457	38.0	38.0	38.0	34.0	38.0
12-13	36.77300328436314	38.0	38.0	38.0	34.5	38.0
14-15	36.78467681078723	38.0	38.0	38.0	35.0	38.0
16-17	36.774636971020584	38.0	38.0	38.0	35.0	38.0
18-19	36.78090727328981	38.0	38.0	38.0	35.0	38.0
20-21	36.808222774930755	38.0	38.0	38.0	35.0	38.0
22-23	36.7859092585813	38.0	38.0	38.0	35.0	38.0
24-25	36.793358113201606	38.0	38.0	38.0	35.0	38.0
26-27	36.759992917001696	38.0	38.0	38.0	35.0	38.0
28-29	36.68497807974754	38.0	38.0	38.0	34.0	38.0
30-31	36.70858378799084	38.0	38.0	38.0	34.0	38.0
32-33	36.70356134382712	38.0	38.0	38.0	34.0	38.0
34-35	36.68771346785962	38.0	38.0	38.0	34.0	38.0
36-37	36.662038058365404	38.0	38.0	38.0	34.0	38.0
38-39	36.64002593543978	38.0	38.0	38.0	34.0	38.0
40-41	36.64998793817223	38.0	38.0	38.0	34.0	38.0
42-43	36.63001834537984	38.0	38.0	38.0	34.0	38.0
44-45	36.57221610858632	38.0	38.0	38.0	34.0	38.0
46-47	36.45496349461858	38.0	38.0	38.0	34.0	38.0
48-49	36.40822079461496	38.0	38.0	38.0	34.0	38.0
50-51	36.41735095407981	38.0	38.0	38.0	34.0	38.0
52-53	36.421748667540825	38.0	38.0	38.0	34.0	38.0
54-55	36.3454049122531	38.0	38.0	38.0	34.0	38.0
56-57	36.36668187341924	38.0	38.0	38.0	34.0	38.0
58-59	36.31056334747714	38.0	38.0	38.0	33.5	38.0
60-61	36.32568475101942	38.0	38.0	38.0	33.0	38.0
62-63	36.34473836516561	38.0	38.0	38.0	33.5	38.0
64-65	36.330663227264445	38.0	38.0	38.0	33.5	38.0
66-67	36.31292037894303	38.0	38.0	38.0	33.0	38.0
68-69	36.292552728758636	38.0	38.0	38.0	33.0	38.0
70-71	36.292670581272624	38.0	38.0	38.0	33.0	38.0
72-73	36.28313566602975	38.0	38.0	38.0	33.0	38.0
74-75	36.24785622352704	38.0	37.5	38.0	33.0	38.0
76-77	36.24264956453096	38.0	37.0	38.0	33.0	38.0
78-79	36.233004539643176	38.0	37.0	38.0	33.0	38.0
80-81	36.24128790529009	38.0	37.0	38.0	33.0	38.0
82-83	36.212564881556986	38.0	37.0	38.0	33.0	38.0
84-85	36.17975524346797	38.0	37.0	38.0	33.0	38.0
86-87	36.17949278793438	38.0	37.0	38.0	33.0	38.0
88-89	36.18653960929606	38.0	37.0	38.0	33.0	38.0
90-91	36.133896285601296	38.0	37.0	38.0	33.0	38.0
92-93	36.154973533926345	38.0	37.0	38.0	33.0	38.0
94-95	36.10945990644486	38.0	37.0	38.0	33.0	38.0
96-97	36.13622577958145	38.0	37.0	38.0	33.0	38.0
98-99	36.18431376077315	38.0	37.0	38.0	33.0	38.0
100-101	35.65246397212235	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	19.0
19	45.0
20	140.0
21	556.0
22	2319.0
23	7106.0
24	16124.0
25	30399.0
26	50835.0
27	77239.0
28	110226.0
29	148057.0
30	190831.0
31	240191.0
32	305394.0
33	395143.0
34	542762.0
35	845349.0
36	1781932.0
37	6144199.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.70315925372966	20.65928977564153	15.823810535327407	23.81374043530141
2	14.343133341029265	21.99384527447249	41.3926184620276	22.270402922470645
3	17.85045811461227	26.505516780484733	31.229460102789368	24.414565002113626
4	12.65937720437265	19.037597017651695	38.133391080377585	30.16963469759807
5	13.499122085118598	37.406635397189866	35.499490746883744	13.594751770807795
6	29.406043806026833	38.53994175886252	18.662189555625943	13.391824879484707
7	25.987872288916737	32.897578094663956	23.075125830558836	18.03942378586047
8	23.916156948898916	37.29486505898822	21.047254769985756	17.74172322212711
9	24.891648526582514	17.38834400524058	21.42713811691554	36.292869351261366
10-11	23.81612819476476	27.209933373245654	28.856316482455618	20.11762194953397
12-13	24.821381800201824	24.608788535226985	29.056982782783297	21.512846881787894
14-15	21.882750173594705	26.042116954478928	26.823855627246502	25.251277244679866
16-17	21.365152799615828	29.216514589348076	27.514441582500442	21.903891028535654
18-19	21.352690531955155	28.112873798004184	29.515834013615187	21.018601656425474
20-21	22.118586457973056	27.19656645202553	29.499382012059993	21.18546507794142
22-23	21.84026972676879	27.232476767974017	29.357075181923527	21.570178323333664
24-25	21.635155720302105	27.389601915890527	29.480199595506868	21.495042768300504
26-27	21.6729708246853	27.489617192084115	29.414753112499533	21.42265887073105
28-29	21.510618990532375	27.399672639626232	29.549984041009438	21.539724328831955
30-31	21.881848796065732	27.334284094294826	29.34821315031415	21.435653959325293
32-33	21.636468868727572	27.388360500095544	29.3758624910135	21.59930814016339
34-35	21.619496470151017	27.520461938044942	29.503630153734367	21.356411438069674
36-37	21.725777711782612	27.3865496696117	29.319724923899326	21.567947694706362
38-39	21.50646672738904	27.420691763743832	29.49127365931501	21.581567849552115
40-41	21.766239123031852	27.288476344478447	29.3800698687955	21.565214663694203
42-43	21.73429356085749	27.412119837536846	29.175604741109616	21.677981860496047
44-45	21.715993739572127	27.568996565013855	29.022768088925854	21.692241606488167
46-47	21.925449308891952	27.48073415639474	28.651533670042895	21.942282864670418
48-49	21.844102403414496	27.60366671879334	28.612598994223443	21.939631883568726
50-51	21.724455253943134	27.74070129717568	28.628671795662164	21.906171653219026
52-53	21.909645576649293	27.669487770730488	28.61180905759503	21.809057595025195
54-55	21.873024722973327	27.636909434169816	28.495647366645287	21.99441847621157
56-57	21.999745528564418	27.77877183286176	28.342283578668294	21.879199059905527
58-59	21.82291889310899	27.948038163204238	28.494366781655273	21.734676162031498
60-61	21.950136270569242	28.075828862605178	28.288124386228418	21.685910480597155
62-63	21.79071093260733	28.213552279327285	28.255318433749228	21.74041835431616
64-65	21.796272091833835	28.153734882495467	28.255787401778193	21.794205623892502
66-67	21.86745731281909	28.122440801253955	28.158885798740087	21.851216087186863
68-69	21.85140628779852	28.035889956311827	28.256832076090284	21.855871679799378
70-71	21.86780051434391	27.714986190450926	28.379683655135747	22.03752964006942
72-73	21.96640913064851	27.65776235516622	28.323449229020515	22.05237928516475
74-75	21.858621305732733	27.792287365199964	28.28992883051436	22.059162498552944
76-77	21.952719973159766	27.740454378526415	28.261296518742775	22.045529129571047
78-79	21.93824043009923	27.764447817196775	28.218006625642154	22.079305127061836
80-81	21.922444305851517	27.78830808627209	28.21036709336467	22.078880514511727
82-83	21.981112492645813	27.776310026387378	28.252487425067375	21.99009005589943
84-85	22.10019665475075	27.770507345566337	28.151646940826126	21.977649058856787
86-87	21.939481683614474	27.791846701949584	28.248454126502736	22.020217487933206
88-89	22.019581248016333	27.766843879730622	28.165964266027622	22.047610606225422
90-91	22.053561040919064	27.82965340224243	28.120995609878925	21.995789946959587
92-93	22.081856646757387	27.807188959951652	28.116310599049076	21.994643794241885
94-95	21.980840785298426	27.868023012419563	28.12814892146649	22.02298728081552
96-97	22.00006397825958	27.897677436848127	28.129589488067293	21.972669096825005
98-99	22.256761281772913	28.42792361017739	28.21707484631386	21.09824026173584
100-101	22.82352533881424	30.99818798226331	25.58417528804747	20.59411139087499
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2827.0
1	2769.0
2	3408.5
3	4381.5
4	5575.5
5	7947.5
6	10249.5
7	13504.5
8	16735.0
9	17970.0
10	17180.5
11	15883.5
12	16973.0
13	21230.5
14	30531.5
15	41969.5
16	50456.5
17	54302.0
18	53589.5
19	50067.5
20	45709.5
21	42185.5
22	42219.0
23	47660.5
24	58443.0
25	74671.5
26	95145.0
27	117847.0
28	141591.5
29	165573.0
30	187693.5
31	210653.5
32	237657.0
33	265936.0
34	291667.0
35	314981.0
36	339685.0
37	362359.5
38	380306.0
39	394305.5
40	408967.5
41	423415.0
42	432574.0
43	442767.5
44	462119.0
45	472574.0
46	465786.5
47	459258.5
48	452574.0
49	442076.0
50	429753.0
51	414294.5
52	393883.5
53	371229.5
54	346577.0
55	317928.5
56	288623.0
57	258682.5
58	227852.5
59	196693.0
60	164142.5
61	132921.0
62	104198.0
63	79894.5
64	60411.0
65	44542.0
66	32542.5
67	24316.0
68	18158.5
69	13102.5
70	9441.5
71	6792.0
72	4732.0
73	3134.0
74	2068.0
75	1326.5
76	831.5
77	526.0
78	336.0
79	207.5
80	134.5
81	98.0
82	68.5
83	48.5
84	37.5
85	24.5
86	17.0
87	15.0
88	10.0
89	6.0
90	4.5
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.05753581937664968
6	1.8367380487358236E-5
7	0.0038295988316141925
8	0.0
9	0.0
10-11	0.0
12-13	9.183690243679118E-6
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	6.89070587104678E-5
28-29	1.8383283087990803E-5
30-31	2.3003760148626373E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	9.525618937806805E-6
46-47	0.0
48-49	0.0
50-51	0.0
52-53	9.205588707981841E-4
54-55	1.5374435448250594E-4
56-57	0.0029595801835509633
58-59	0.007621413348764343
60-61	0.003364654950314669
62-63	0.005779597508419613
64-65	0.0069480156699708055
66-67	0.0051301913795997
68-69	0.003728042069164448
70-71	0.005395706135370179
72-73	0.0032946526068307227
74-75	0.002873699549290047
76-77	0.0026977405519982107
78-79	8.288815028793133E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	4.601967490666203E-5
88-89	0.0
90-91	2.0575802667940877E-4
92-93	0.0
94-95	1.0223882580753338E-4
96-97	6.093167207597741E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	176.0
20-21	754.0
22-23	1414.0
24-25	1667.0
26-27	3666.0
28-29	8557.0
30-31	20512.0
32-33	26776.0
34-35	37010.0
36-37	56741.0
38-39	67056.0
40-41	67078.0
42-43	77168.0
44-45	91764.0
46-47	92341.0
48-49	84608.0
50-51	76539.0
52-53	73902.0
54-55	79161.0
56-57	82164.0
58-59	81648.0
60-61	80179.0
62-63	78462.0
64-65	87055.0
66-67	94135.0
68-69	93230.0
70-71	101751.0
72-73	81397.0
74-75	82878.0
76-77	87945.0
78-79	91572.0
80-81	91029.0
82-83	87142.0
84-85	87133.0
86-87	90977.0
88-89	94320.0
90-91	96380.0
92-93	93865.0
94-95	102212.0
96-97	161728.0
98-99	896590.0
100-101	7178187.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.68353981847608
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.66436747373486	75.93096033260498
2	7.1225005240731925	12.06317113474938
3	1.7561310601305051	4.461461836710727
4	0.6773013137445345	2.2942509108636577
5	0.2985354096650664	1.264051762579836
6	0.15257063807247603	0.7752133022604497
7	0.0970358432459885	0.5752137086738749
8	0.05879125859114784	0.3982921510305434
9	0.0356135327412176	0.2714292016170752
>10	0.1340972096188844	1.7220319597885534
>50	0.0025546781374911074	0.14095348161890692
>100	4.467136253970272E-4	0.06658393515989917
>500	3.623122037799335E-5	0.020893824278566772
>1k	1.8113398923307623E-5	0.015492458063595617
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.673476097471647E-5	0.0	0.0	0.0
3	0.0	3.673476097471647E-5	0.0	9.183690243679118E-6	0.0
4	0.0	4.591845121839559E-5	0.0	1.8367380487358236E-5	0.0
5	1.8367380487358236E-5	4.591845121839559E-5	0.0	1.8367380487358236E-5	0.0
6	7.346952194943294E-5	4.591845121839559E-5	0.0	1.8367380487358236E-5	1.8367380487358236E-5
7	7.346952194943294E-5	4.591845121839559E-5	0.0	1.8367380487358236E-5	3.673476097471647E-5
8	7.346952194943294E-5	4.591845121839559E-5	0.0	1.8367380487358236E-5	3.673476097471647E-5
9	7.346952194943294E-5	1.010205926804703E-4	0.0	5.510214146207471E-5	5.510214146207471E-5
10-11	7.346952194943294E-5	1.010205926804703E-4	0.0	7.80613670712725E-5	5.510214146207471E-5
12-13	7.346952194943294E-5	1.010205926804703E-4	0.0	9.183690243679118E-5	6.428583170575383E-5
14-15	1.010205926804703E-4	1.9285749511726148E-4	0.0	1.0561243780230986E-4	8.265321219311206E-5
16-17	1.2857166341150765E-4	2.020411853609406E-4	0.0	1.2857166341150765E-4	8.265321219311206E-5
18-19	1.2857166341150765E-4	2.1122487560461972E-4	0.0	1.5153088902070545E-4	9.183690243679118E-5
20-21	1.2857166341150765E-4	2.2040856584829883E-4	0.0	1.8826564999542192E-4	1.010205926804703E-4
22-23	1.3775535365518677E-4	2.479596365793362E-4	0.0	1.9744934023910104E-4	1.1479612804598898E-4
24-25	1.3775535365518677E-4	2.892862426758922E-4	0.0	2.2040856584829883E-4	1.3775535365518677E-4
26-27	1.469390438988659E-4	3.0765362316325046E-4	0.0	2.801025524322131E-4	1.3775535365518677E-4
28-29	1.7449011462990324E-4	3.1224546828509E-4	0.0	5.051029634023515E-4	1.3775535365518677E-4
30-31	1.8367380487358236E-4	3.811231451126834E-4	0.0	0.0011387775902162106	1.3775535365518677E-4
32-33	1.9285749511726148E-4	3.8571499023452296E-4	0.0	0.002736739692616377	1.469390438988659E-4
34-35	2.020411853609406E-4	4.4081713169659767E-4	0.0	0.004821437377931537	1.469390438988659E-4
36-37	2.020411853609406E-4	4.959192731586724E-4	0.0	0.007907157299807722	1.469390438988659E-4
38-39	2.020411853609406E-4	5.188784987678702E-4	0.0	0.012471451350916242	1.469390438988659E-4
40-41	2.020411853609406E-4	6.153072463265009E-4	0.0	0.017338807180066175	1.469390438988659E-4
42-43	2.020411853609406E-4	6.382664719356987E-4	0.0	0.022160244557997713	1.469390438988659E-4
44-45	2.020411853609406E-4	7.209196841288108E-4	0.0	0.027739336381032778	1.469390438988659E-4
46-47	2.020411853609406E-4	7.897973609564042E-4	0.0	0.03371332688454604	1.6530642438622413E-4
48-49	2.0663303048278016E-4	7.897973609564042E-4	0.0	0.03993986886976049	1.8367380487358236E-4
50-51	2.250004109701384E-4	8.586750377839975E-4	0.0	0.04605620657205078	1.8367380487358236E-4
52-53	2.2959225609197795E-4	8.678587280276767E-4	0.0	0.05191540094751806	1.8826564999542192E-4
54-55	2.341841012138175E-4	8.816342633931953E-4	0.0	0.05786184038030029	1.9285749511726148E-4
56-57	2.3877594633565707E-4	9.000016438805536E-4	0.0	0.06419399480331703	1.9744934023910104E-4
58-59	2.3877594633565707E-4	9.091853341242327E-4	0.0	0.07027818958975446	2.020411853609406E-4
60-61	2.479596365793362E-4	9.551037853426283E-4	0.0	0.0764128946725321	2.020411853609406E-4
62-63	2.571433268230153E-4	9.87246701195505E-4	0.0	0.08232259934433961	2.2040856584829883E-4
64-65	2.571433268230153E-4	0.001166328660947248	0.0	0.08782822164542525	2.2040856584829883E-4
66-67	2.571433268230153E-4	0.0011755123511909271	0.0	0.09383894690991323	2.2040856584829883E-4
68-69	2.571433268230153E-4	0.001198471576800125	0.0	0.09960630438294371	2.2040856584829883E-4
70-71	2.70918862188534E-4	0.0012168389572874833	0.0	0.10511651852915119	2.2040856584829883E-4
72-73	2.938780877977318E-4	0.0012352063377748415	0.0	0.10916193408149184	2.3877594633565707E-4
74-75	2.938780877977318E-4	0.0012673492536277183	0.0	0.11223847031312435	2.3877594633565707E-4
76-77	2.938780877977318E-4	0.0013040840146024348	0.0	0.11512674089476144	2.3877594633565707E-4
78-79	2.938780877977318E-4	0.001322451395089793	0.0	0.1174685819068996	2.3877594633565707E-4
80-81	2.938780877977318E-4	0.001322451395089793	0.0	0.11858899211662846	2.479596365793362E-4
82-83	2.9846993291957134E-4	0.0013270432402116327	0.0	0.11896093157149747	2.571433268230153E-4
84-85	3.030617780414109E-4	0.0013408187755771512	0.0	0.11933287102636647	2.571433268230153E-4
86-87	3.030617780414109E-4	0.0013500024658208304	0.0	0.11952113667636188	2.571433268230153E-4
88-89	3.030617780414109E-4	0.0014234719877702633	0.0	0.11957164697270212	2.571433268230153E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16035	0.0	46.39988	1
GTATCAA	28350	0.0	36.26439	1
TCAACGC	34515	0.0	29.53713	4
ATCAACG	34655	0.0	29.417807	3
CAACGCA	35265	0.0	28.84783	5
AACGCAG	36515	0.0	27.903296	6
TATCAAC	36875	0.0	27.868864	2
CTTATAC	8305	0.0	26.47132	1
ACGCAGA	41330	0.0	24.642103	7
CGCAGAG	41835	0.0	24.333225	8
GCAGAGT	48770	0.0	20.917253	9
GTGGTAT	8720	0.0	20.317507	1
TATACAC	12715	0.0	19.866726	3
TGGTATC	8400	0.0	19.859884	2
GAGTACT	30040	0.0	19.647005	12-13
AGAGTAC	43835	0.0	19.370522	10-11
CAGAGTA	47150	0.0	18.588816	10-11
TATGCCG	6965	0.0	17.895823	42-43
GTACATG	31055	0.0	17.87843	1
AGTACTT	32565	0.0	17.806137	12-13
>>END_MODULE