FastQCFastQC Report
Fri 27 May 2016
SRR2139696_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139696_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10917345
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148650.052.454381
GTATCAA262250.035.7711261
TCAACGC328950.028.3998854
ATCAACG331100.028.2024353
CAACGCA337900.027.6731935
TATCAAC345850.027.2242242
AACGCAG350300.026.7638956
CTTATAC78150.024.1290911
ACGCAGA398950.023.4785487
CGCAGAG403300.023.2031768
GTGGTAT72150.020.8128451
TGGTATC67100.020.6429162
GCAGAGT471050.019.939169
GAGTACT291650.019.70249412-13
TATACAC129750.019.122693
AGAGTAC429450.018.80291610-11
CAGAGTA461300.018.64050510-11
GTACATG312250.018.365851
TACATGG307850.018.221862
ACATGGG310300.017.632993
Produced by FastQC (version 0.11.5)