FastQCFastQC Report
Fri 27 May 2016
SRR2139695_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139695_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14556142
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA195850.052.0851481
GTATCAA356450.037.2977941
CTTATAC124850.031.5207351
TCAACGC452950.029.122944
ATCAACG454450.029.0458053
CAACGCA464550.028.3585725
TATCAAC475150.027.9983732
AACGCAG480100.027.4954286
TATACAC186100.024.717293
ACGCAGA549250.024.0416347
CGCAGAG555650.023.7243678
TGGTATC88750.023.4352022
GTGGTAT94100.023.4326441
TTATACA187450.022.5365432
GCAGAGT643300.020.5120189
ACACATC203750.019.8745296
ACATCTC203650.019.7135948
GAGTACT404600.019.50942212-13
AGAGTAC589300.019.2048410-11
TACACAT238700.019.0898065
Produced by FastQC (version 0.11.5)