##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139695_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14556142
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.38331434249542	31.0	25.0	33.0	18.0	33.0
2	31.009413826823067	33.0	31.0	33.0	27.0	33.0
3	31.128894180889414	33.0	31.0	33.0	27.0	34.0
4	31.89762891843182	33.0	32.0	33.0	29.0	34.0
5	32.345981716858766	33.0	33.0	34.0	31.0	34.0
6	35.93389883116007	38.0	36.0	38.0	31.0	38.0
7	36.23491671076031	38.0	37.0	38.0	33.0	38.0
8	36.488089426442805	38.0	37.0	38.0	34.0	38.0
9	36.693812343957624	38.0	38.0	38.0	34.0	38.0
10-11	36.72034303457606	38.0	38.0	38.0	34.0	38.0
12-13	36.76874689735783	38.0	38.0	38.0	34.0	38.0
14-15	36.779896280209414	38.0	38.0	38.0	35.0	38.0
16-17	36.76724275566974	38.0	38.0	38.0	34.5	38.0
18-19	36.77269605503986	38.0	38.0	38.0	34.5	38.0
20-21	36.80045083743752	38.0	38.0	38.0	35.0	38.0
22-23	36.77819194699572	38.0	38.0	38.0	35.0	38.0
24-25	36.78242856188836	38.0	38.0	38.0	35.0	38.0
26-27	36.75065678270417	38.0	38.0	38.0	34.5	38.0
28-29	36.674343675257674	38.0	38.0	38.0	34.0	38.0
30-31	36.69933806763639	38.0	38.0	38.0	34.0	38.0
32-33	36.693205788017345	38.0	38.0	38.0	34.0	38.0
34-35	36.674993207810864	38.0	38.0	38.0	34.0	38.0
36-37	36.649905754845214	38.0	38.0	38.0	34.0	38.0
38-39	36.6274286660438	38.0	38.0	38.0	34.0	38.0
40-41	36.639575562060486	38.0	38.0	38.0	34.0	38.0
42-43	36.618820007657746	38.0	38.0	38.0	34.0	38.0
44-45	36.55939894121627	38.0	38.0	38.0	34.0	38.0
46-47	36.44047897622099	38.0	38.0	38.0	34.0	38.0
48-49	36.39336285028773	38.0	38.0	38.0	34.0	38.0
50-51	36.39952829731973	38.0	38.0	38.0	34.0	38.0
52-53	36.4025870068355	38.0	38.0	38.0	34.0	38.0
54-55	36.327034072749186	38.0	38.0	38.0	33.0	38.0
56-57	36.346912477870944	38.0	38.0	38.0	34.0	38.0
58-59	36.290331089725356	38.0	38.0	38.0	33.5	38.0
60-61	36.302054111918274	38.0	38.0	38.0	33.0	38.0
62-63	36.31831640986546	38.0	38.0	38.0	33.0	38.0
64-65	36.30668731146778	38.0	38.0	38.0	33.0	38.0
66-67	36.286510483721955	38.0	38.0	38.0	33.0	38.0
68-69	36.26755280402195	38.0	37.5	38.0	33.0	38.0
70-71	36.26660635246573	38.0	37.0	38.0	33.0	38.0
72-73	36.254861666967706	38.0	37.0	38.0	33.0	38.0
74-75	36.21809067971756	38.0	37.0	38.0	33.0	38.0
76-77	36.21101443961709	38.0	37.0	38.0	33.0	38.0
78-79	36.19979031710728	38.0	37.0	38.0	33.0	38.0
80-81	36.2076325255199	38.0	37.0	38.0	33.0	38.0
82-83	36.17809952224512	38.0	37.0	38.0	33.0	38.0
84-85	36.14639802896659	38.0	37.0	38.0	33.0	38.0
86-87	36.14740601163456	38.0	37.0	38.0	33.0	38.0
88-89	36.15436031564478	38.0	37.0	38.0	33.0	38.0
90-91	36.1009939783642	38.0	37.0	38.0	33.0	38.0
92-93	36.12276590792651	38.0	37.0	38.0	33.0	38.0
94-95	36.0776555959996	38.0	37.0	38.0	33.0	38.0
96-97	36.10543167583385	38.0	37.0	38.0	33.0	38.0
98-99	36.15335677723381	38.0	37.0	38.0	33.0	38.0
100-101	35.61083109332175	38.0	36.0	38.0	30.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	8.0
18	35.0
19	60.0
20	165.0
21	872.0
22	3394.0
23	9630.0
24	22299.0
25	41612.0
26	69088.0
27	105284.0
28	149725.0
29	199966.0
30	257761.0
31	326193.0
32	412736.0
33	534112.0
34	733899.0
35	1140986.0
36	2389512.0
37	8158805.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.65262224015127	20.746740448121486	15.799777166229898	23.800860145497342
2	14.34825931211718	21.97361086474699	41.42572255752932	22.252407265606504
3	17.79500364863162	26.545303006799465	31.310130115520995	24.349563229047916
4	12.61622069913855	19.091130053554025	38.04560988756499	30.247039359742438
5	13.463748709410886	37.45187872655458	35.55989227103815	13.52448029299638
6	29.399026198013186	38.489924047182285	18.65716891192735	13.453880842877187
7	25.977773168647694	32.941817579990065	22.98026381643803	18.100145434924208
8	23.942401771018723	37.340780269936914	21.052886128755823	17.663931830288547
9	24.930424558925026	17.298876309395716	21.411799912366888	36.35889921931237
10-11	23.830229191223886	27.20422073376311	28.8611158093951	20.10443426561791
12-13	24.758809717574888	24.595088451321786	29.079673034242177	21.566428796861146
14-15	21.860462751737376	26.044346091155195	26.754747240031048	25.340443917076378
16-17	21.378810401822133	29.254503013229744	27.448457839996337	21.918228744951787
18-19	21.38459490158862	28.109223584106285	29.43546098959463	21.070720524710463
20-21	22.09226394984386	27.171494767799505	29.474775254939157	21.261466027417477
22-23	21.82684601339432	27.259636508544503	29.322671237935577	21.590846240125593
24-25	21.63371070988857	27.397043163098445	29.451107022955764	21.51813910405722
26-27	21.61118040515072	27.467301649548652	29.423379778037084	21.498138167263544
28-29	21.418435727455545	27.364301339708074	29.583127521991102	21.634135410845275
30-31	21.7529663165607	27.228580980781942	29.32983623121	21.688616471447357
32-33	21.69311806268693	27.36862746085114	29.460302011084426	21.477952465377502
34-35	21.772816901992723	27.543301746035915	29.361121213084967	21.322760138886395
36-37	21.588230648268482	27.4053402313334	29.31545233355084	21.690976786847273
38-39	21.47961723675956	27.439349480986724	29.495766681154013	21.5852666010997
40-41	21.73374939013047	27.305489352292234	29.399130211971343	21.561631045605957
42-43	21.739753635050914	27.353504487234666	29.221422743575847	21.68531913413857
44-45	21.687798382254776	27.57773139217003	29.03098814120656	21.703482084368638
46-47	21.96295819354823	27.439193143310675	28.635425383322232	21.962423279818864
48-49	21.822729505161124	27.564929887554463	28.62429282506294	21.988047782221475
50-51	21.68975147798396	27.701988890665298	28.64415223199198	21.964107399358763
52-53	21.89328391411065	27.63589771638259	28.68984585825099	21.780972511255765
54-55	21.87319119513127	27.640893056921602	28.50495430658552	21.980961441361604
56-57	21.92309302556235	27.85483188600501	28.344087392097777	21.877987696334863
58-59	21.76855237093185	28.04007476445375	28.519966581414764	21.671406283199637
60-61	21.968693593035525	28.174233919720805	28.244668840755367	21.612403646488307
62-63	21.787749424748217	28.366183221779373	28.22508960842115	21.620977745051256
64-65	21.790515994711424	28.276817232803097	28.184745612872707	21.747921159612773
66-67	21.80735655110571	28.24149528768186	28.138192199854196	21.81295596135823
68-69	21.79066184682796	28.120733013162003	28.231664352943888	21.85694078706615
70-71	21.860835513899797	27.767898387913746	28.35816803890617	22.013098059280285
72-73	21.946021567148236	27.708261836219663	28.32830460545694	22.017411991175166
74-75	21.843697321341786	27.850815800989277	28.289404248667697	22.01608262900124
76-77	21.99062517924326	27.74083471512353	28.238802106781346	22.02973799885186
78-79	21.903835790301247	27.772262699054824	28.208670028236277	22.11523148240765
80-81	21.925575097485467	27.805172978502302	28.210618523407412	22.058633400604823
82-83	21.97928201302193	27.801483458638266	28.24712790731733	21.972106621022476
84-85	22.049391426204874	27.795350681743823	28.137640905317824	22.017616986733483
86-87	21.93946502693656	27.827162892102976	28.21334867271815	22.020023408242313
88-89	22.001564369330815	27.79616271956522	28.137925590753266	22.064347320350702
90-91	22.03576776500275	27.852079207330327	28.137688802184968	21.97446422548196
92-93	22.06167110576586	27.778645493804333	28.139738053235487	22.01994534719432
94-95	21.97270721115646	27.888094488163546	28.113342484336883	22.02585581634311
96-97	22.02962001383384	27.895037317785555	28.130860619630795	21.944482048749812
98-99	22.2466531707567	28.4448922765818	28.207836388353098	21.1006181643084
100-101	22.816385339954238	30.969275331595263	25.591848150110465	20.622491178340034
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4343.0
1	4074.5
2	4503.0
3	5557.5
4	7079.5
5	10297.5
6	13439.5
7	17698.5
8	22178.5
9	23823.0
10	22645.0
11	21056.5
12	22597.5
13	28248.5
14	40709.5
15	56244.5
16	67165.5
17	71731.0
18	70816.5
19	66136.5
20	60590.5
21	56431.0
22	56645.0
23	63553.5
24	77071.5
25	97935.0
26	125053.0
27	154122.0
28	185054.5
29	218307.5
30	250252.0
31	284042.0
32	320163.0
33	356381.0
34	391443.0
35	423373.0
36	457752.0
37	488389.5
38	512154.5
39	532919.0
40	551479.5
41	568216.0
42	578816.0
43	587892.0
44	599281.5
45	619654.0
46	627423.5
47	616734.5
48	606433.5
49	589367.5
50	570038.5
51	548422.0
52	518903.5
53	487905.5
54	457327.5
55	420824.0
56	382295.0
57	344852.0
58	306540.0
59	264380.5
60	218027.0
61	174986.5
62	137377.0
63	105186.0
64	78676.0
65	57923.0
66	42598.0
67	31992.5
68	24273.0
69	18017.0
70	13075.0
71	9269.5
72	6457.0
73	4217.0
74	2755.0
75	1809.0
76	1107.5
77	656.0
78	419.0
79	268.5
80	171.0
81	111.0
82	81.0
83	59.0
84	39.0
85	26.5
86	20.0
87	14.5
88	8.5
89	4.0
90	3.5
91	3.0
92	3.0
93	3.0
94	3.0
95	2.5
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.05854573279101015
6	0.0
7	0.0033731465384165666
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	7.216334151734353E-5
28-29	3.094044833465959E-5
30-31	3.44135103311939E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	1.067644686140221E-5
46-47	0.0
48-49	0.0
50-51	3.649320449091213E-6
52-53	8.527580842936663E-4
54-55	1.5549635175940421E-4
56-57	0.002999966642161964
58-59	0.0077415935810828855
60-61	0.003269408557544151
62-63	0.005647708414588447
64-65	0.006580792782362273
66-67	0.0050738432911593455
68-69	0.003749717592024123
70-71	0.005204403338398291
72-73	0.0031383831523565824
74-75	0.00280546879383545
76-77	0.002524661819915137
78-79	5.774708254520596E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	4.7213052159864085E-5
88-89	0.0
90-91	1.5347786768024036E-4
92-93	0.0
94-95	8.97097772084596E-5
96-97	9.08865303935237E-6
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	169.0
20-21	890.0
22-23	1789.0
24-25	2162.0
26-27	4874.0
28-29	10769.0
30-31	26638.0
32-33	34558.0
34-35	48241.0
36-37	73564.0
38-39	86267.0
40-41	87301.0
42-43	100257.0
44-45	119971.0
46-47	121572.0
48-49	110567.0
50-51	100079.0
52-53	95873.0
54-55	103727.0
56-57	107967.0
58-59	108330.0
60-61	107736.0
62-63	105610.0
64-65	117381.0
66-67	128213.0
68-69	125579.0
70-71	140321.0
72-73	108046.0
74-75	109955.0
76-77	115698.0
78-79	121279.0
80-81	120641.0
82-83	115617.0
84-85	115916.0
86-87	119040.0
88-89	125233.0
90-91	128220.0
92-93	125986.0
94-95	136327.0
96-97	217480.0
98-99	1200402.0
100-101	9625897.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.11848758416927
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.44691987374411	75.24139618841727
2	7.270002993226401	12.23083313045177
3	1.7949437274031639	4.529638549435368
4	0.6691480754278794	2.2515089629940332
5	0.3047760577502896	1.28186505149099
6	0.15750810935913892	0.7949606364919622
7	0.10030227192574803	0.5906092790955003
8	0.061488408541851475	0.41378495443984487
9	0.041760052347418065	0.3161513200410491
>10	0.14828266607501187	1.9649660047512019
>50	0.003804222698158889	0.207409818618906
>100	0.0010197960128282694	0.13621450459611545
>500	1.4582413715414627E-5	0.009592407590866481
>1k	2.9163074283885316E-5	0.031069191585119175
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.869952216734352E-6
2	0.0	2.0609856650203057E-5	0.0	6.869952216734352E-6	1.3739904433468704E-5
3	0.0	2.0609856650203057E-5	0.0	1.3739904433468704E-5	1.3739904433468704E-5
4	0.0	6.182956995060916E-5	0.0	1.3739904433468704E-5	1.3739904433468704E-5
5	0.0	6.182956995060916E-5	0.0	2.7479808866937408E-5	1.3739904433468704E-5
6	0.0	6.182956995060916E-5	0.0	3.434976108367176E-5	2.0609856650203057E-5
7	6.869952216734352E-6	6.182956995060916E-5	0.0	4.121971330040611E-5	3.434976108367176E-5
8	6.869952216734352E-6	6.182956995060916E-5	0.0	4.121971330040611E-5	4.121971330040611E-5
9	6.869952216734352E-6	1.0304928325101528E-4	0.0	4.121971330040611E-5	4.808966551714046E-5
10-11	6.869952216734352E-6	1.0304928325101528E-4	0.0	6.182956995060916E-5	4.808966551714046E-5
12-13	6.869952216734352E-6	1.0991923546774963E-4	0.0	7.556947438407787E-5	4.808966551714046E-5
14-15	6.869952216734352E-6	2.0953354261039772E-4	0.0	9.617933103428092E-5	6.869952216734352E-5
16-17	6.869952216734352E-6	2.1983847093549926E-4	0.0	1.0304928325101528E-4	8.243942660081223E-5
18-19	6.869952216734352E-6	2.404483275857023E-4	0.0	1.1678918768448398E-4	9.617933103428092E-5
20-21	6.869952216734352E-6	2.610581842359054E-4	0.0	1.2365913990121833E-4	1.0648425935938245E-4
22-23	6.869952216734352E-6	3.297577064032489E-4	0.0	1.3739904433468703E-4	1.3396406822631986E-4
24-25	6.869952216734352E-6	3.641074674869206E-4	0.0	1.6831382930999162E-4	1.6487885320162445E-4
26-27	6.869952216734352E-6	3.70977419703655E-4	0.0	2.267084231522336E-4	1.717488054183588E-4
28-29	6.869952216734352E-6	3.70977419703655E-4	0.0	4.259370374375298E-4	1.717488054183588E-4
30-31	1.3739904433468704E-5	4.4998187019610003E-4	0.0	0.0011644569007364725	1.717488054183588E-4
32-33	1.3739904433468704E-5	4.568518224128344E-4	0.0	0.002593406961817218	1.7861875763509313E-4
34-35	1.3739904433468704E-5	5.324212967969123E-4	0.0	0.004595998032995281	1.9235866206856184E-4
36-37	1.3739904433468704E-5	6.079907711809901E-4	0.0	0.007649691793333701	1.9579363817692904E-4
38-39	1.3739904433468704E-5	6.182956995060917E-4	0.0	0.012197600160811842	1.992286142852962E-4
40-41	1.3739904433468704E-5	6.869952216734352E-4	0.0	0.01719892537459445	2.0609856650203055E-4
42-43	1.3739904433468704E-5	7.076050783236382E-4	0.0	0.021997586997983393	2.0609856650203055E-4
44-45	1.3739904433468704E-5	8.037844093579192E-4	0.0	0.027280580252652113	2.0609856650203055E-4
46-47	1.3739904433468704E-5	8.79353883741997E-4	0.0	0.03311660466076795	2.0609856650203055E-4
48-49	1.3739904433468704E-5	8.79353883741997E-4	0.0	0.03929612667972049	2.1983847093549926E-4
50-51	1.3739904433468704E-5	9.824031669930123E-4	0.0	0.04551686841197344	2.1983847093549926E-4
52-53	1.3739904433468704E-5	9.892731192097466E-4	0.0	0.05168265052649253	2.3357837536896797E-4
54-55	1.3739904433468704E-5	9.995780475348481E-4	0.0	0.05782438780825304	2.507532559108038E-4
56-57	1.3739904433468704E-5	0.0010304928325101529	0.0	0.06416535370429885	2.54188232019171E-4
58-59	1.3739904433468704E-5	0.0010407977608352544	0.0	0.07073989797571362	2.54188232019171E-4
60-61	1.3739904433468704E-5	0.0010648425935938246	0.0	0.07666179678653863	2.54188232019171E-4
62-63	1.3739904433468704E-5	0.0010854524502440276	0.0	0.08270391976115649	2.54188232019171E-4
64-65	1.3739904433468704E-5	0.0011781968051699414	0.0	0.08856055402592253	2.54188232019171E-4
66-67	1.3739904433468704E-5	0.0011816317812783086	0.0	0.09466794154659935	2.6449316034427256E-4
68-69	1.3739904433468704E-5	0.001209111590145246	0.0	0.10076502413895111	2.6792813645263973E-4
70-71	1.3739904433468704E-5	0.001229721446795449	0.0	0.10608580213081187	2.816680408861084E-4
72-73	1.3739904433468704E-5	0.0012365913990121833	0.0	0.11025586312636962	3.0914784975304583E-4
74-75	1.3739904433468704E-5	0.0012743761362042222	0.0	0.11374579885247066	3.0914784975304583E-4
76-77	1.3739904433468704E-5	0.0013568155628050345	0.0	0.11658652409409032	3.0914784975304583E-4
78-79	1.3739904433468704E-5	0.0013946002999970734	0.0	0.11953373359506936	3.0914784975304583E-4
80-81	1.3739904433468704E-5	0.0014014702522138077	0.0	0.12085619939679071	3.1601780196978017E-4
82-83	1.3739904433468704E-5	0.001408340204430542	0.0	0.12136114088472069	3.3662765861998325E-4
84-85	1.3739904433468704E-5	0.001408340204430542	0.0	0.1218729523248674	3.3662765861998325E-4
86-87	1.3739904433468704E-5	0.0014186451327556436	0.0	0.12209966074801964	3.400626347283504E-4
88-89	1.3739904433468704E-5	0.0014770397265978857	0.0	0.12216836027018697	3.538025391618191E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19585	0.0	52.085148	1
GTATCAA	35645	0.0	37.297794	1
CTTATAC	12485	0.0	31.520735	1
TCAACGC	45295	0.0	29.12294	4
ATCAACG	45445	0.0	29.045805	3
CAACGCA	46455	0.0	28.358572	5
TATCAAC	47515	0.0	27.998373	2
AACGCAG	48010	0.0	27.495428	6
TATACAC	18610	0.0	24.71729	3
ACGCAGA	54925	0.0	24.041634	7
CGCAGAG	55565	0.0	23.724367	8
TGGTATC	8875	0.0	23.435202	2
GTGGTAT	9410	0.0	23.432644	1
TTATACA	18745	0.0	22.536543	2
GCAGAGT	64330	0.0	20.512018	9
ACACATC	20375	0.0	19.874529	6
ACATCTC	20365	0.0	19.713594	8
GAGTACT	40460	0.0	19.509422	12-13
AGAGTAC	58930	0.0	19.20484	10-11
TACACAT	23870	0.0	19.089806	5
>>END_MODULE