FastQCFastQC Report
Fri 27 May 2016
SRR2139694_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139694_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13559157
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA195850.052.703261
GTATCAA349550.036.2420731
TCAACGC436650.028.637374
ATCAACG438400.028.5230563
CAACGCA449200.027.8372825
TATCAAC452950.027.8258422
AACGCAG461200.027.1709586
ACGCAGA523450.023.923257
GTGGTAT87950.023.6390251
CGCAGAG533450.023.458028
TGGTATC83450.023.2080352
GCAGAGT617300.020.2506859
GAGTACT380750.019.86330212-13
CTTATAC93700.019.1041131
CAGAGTA604650.018.82791910-11
AGAGTAC563200.018.80913710-11
GTACATG395850.018.3279441
TACATGG394500.018.0407812
GTACTTT423650.017.74038914-15
AGTACTT415450.017.40012212-13
Produced by FastQC (version 0.11.5)